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1. 发明申请

US20100081159A1 Profiling reactive oxygen, nitrogen and halogen species 审中-公开
标题翻译：分析活性氧，氮和卤素
公开(公告)号：US20100081159A1
公开(公告)日：2010-04-01
申请号：US12286103
申请日：2008-09-26
申请人： Irina V. Lebedeva , Wayne Forrest Patton
发明人： Irina V. Lebedeva , Wayne Forrest Patton
IPC分类号： C12Q1/02
CPC分类号： G01N33/582 , C12Q1/02 , G01N15/14 , G01N33/5005
摘要： This invention provides methods, kits and systems which permit simultaneous profiling of multiple reactive oxygen species (ROS), reactive nitrogen species (RNS) and/or reactive halogen species (RHS) including reactive chlorine species (RCS) and/or reactive bromine species (RBS) through multiplexed fluorescence detection of three or more indicator probes in live cells or subcellular organelles.
摘要翻译：本发明提供方法，试剂盒和系统，其允许包括反应性氯物质（RCS）和/或反应性溴物质（RCS）和/或反应性溴物质的多种活性氧（ROS），活性氮物质（RNS）和/或反应性卤素物质（RHS） RBS）通过活细胞或亚细胞器中的三个或更多个指示物探针的多重荧光检测。

2. 发明授权

US08828678B2 Self-immolative probes for enzyme activity detection 有权
标题翻译：用于酶活性检测的自发性探针
公开(公告)号：US08828678B2
公开(公告)日：2014-09-09
申请号：US12927497
申请日：2010-11-16
申请人： Maciej Szczepanik , Irina V. Lebedeva , Yuejun Xiang , Praveen Pande , Wayne Forrest Patton
发明人： Maciej Szczepanik , Irina V. Lebedeva , Yuejun Xiang , Praveen Pande , Wayne Forrest Patton
IPC分类号： C12Q1/26 , C07D311/02 , C07D305/14 , C07D413/00 , G01N21/00
CPC分类号： C12Q1/26 , C07D311/08 , C07D491/052 , C07D491/20 , C07D493/10 , C09K11/06 , C09K2211/1022 , C09K2211/1088 , C12Q1/37 , C12Q1/44 , C12Q1/485 , G01N2333/902 , G01N2500/00 , Y10T436/18
摘要： A compound comprising the structure: (SIG)-(SI-MOD)m. is provided in which SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. When MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided are methods of determining whether a sample comprises an activator such as a nitroreductase, and methods of determining whether a mammalian cell is hypoxic using a compound of the formula (SIG)-(SI-MOD)m.
摘要翻译：包含以下结构的化合物：（SIG） - （SI-MOD）m。其中SIG是信号分子，SI是与SIG结合的自消除结构，使得SIG相对于没有SI的SIG的信号具有减少的信号，MOD是与SI结合的部分，其经受活化剂，m是1至约10的整数。当通过活化剂修饰MOD时，SI是不稳定的并且从SIG自分离，使得SIG产生增加的信号。还提供了确定样品是否包含诸如硝酸还原酶的活化剂的方法以及使用式（SIG） - （SI-MOD）m的化合物来确定哺乳动物细胞是否缺氧的方法。

3. 发明申请

US20120122136A1 Self-immolative probes for enzyme activity detection 有权
标题翻译：用于酶活性检测的自发性探针
公开(公告)号：US20120122136A1
公开(公告)日：2012-05-17
申请号：US12927497
申请日：2010-11-16
申请人： Maciej Szczepanik , Irina V. Lebedeva , Yuejun Xiang , Praveen Pande , Wayne Forrest Patton
发明人： Maciej Szczepanik , Irina V. Lebedeva , Yuejun Xiang , Praveen Pande , Wayne Forrest Patton
IPC分类号： C12Q1/26 , C07D491/22 , G01N21/75 , C07D493/10 , C07D413/14 , C07D237/32 , C07D311/08
CPC分类号： C12Q1/26 , C07D311/08 , C07D491/052 , C07D491/20 , C07D493/10 , C09K11/06 , C09K2211/1022 , C09K2211/1088 , C12Q1/37 , C12Q1/44 , C12Q1/485 , G01N2333/902 , G01N2500/00 , Y10T436/18
摘要： Provided is a compound comprising the structure: (SIG)-(SI-MOD)m. In this compound, SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. With this compound, when MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided is a method of determining whether a sample comprises an activator, using the above-described compound. Additionally provided is a method of determining whether a cell comprises a nitroreductase using the above-described compound where nitroreductase is the activator. Further provided is a method of determining whether a mammalian cell is hypoxic using the above-described compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase, using the above-described compound where nitroreductase is the activator, is also provided. Also provided is a method of identifying nitroreductase in a sample, using the above-described compound where nitroreductase is the activator.
摘要翻译：提供了包含以下结构的化合物：（SIG） - （SI-MOD）m。在该化合物中，SIG是信号分子，SI是与SIG结合的自消除结构，使得SIG相对于没有SI的SIG的信号具有降低的信号，MOD是与SI结合的部分，其经受活化剂，m为1至约10的整数。用该化合物，当MOD被活化剂修饰时，SI是不稳定的并且从SIG自分离，使得SIG产生增加的信号。还提供了使用上述化合物来确定样品是否包含活化剂的方法。另外提供了使用上述硝基还原酶是活化剂的化合物来确定细胞是否包含硝基还原酶的方法。还提供了使用上述硝基还原酶是活化剂的化合物来确定哺乳动物细胞是否缺氧的方法。还提供了使用上述硝基还原酶为活化剂的化合物检测包含硝基还原酶的微生物的方法。还提供了使用上述硝酸还原酶是活化剂的化合物来鉴定样品中的硝基还原酶的方法。

4. 发明授权

US08445271B2 Processes and kits for determining multi-drug resistance of cells 有权
公开(公告)号：US08445271B2
公开(公告)日：2013-05-21
申请号：US12799853
申请日：2010-05-03
申请人： Irina Lebedeva , Praveen Pande , Wayne Forrest Patton
发明人： Irina Lebedeva , Praveen Pande , Wayne Forrest Patton
IPC分类号： C12N15/00
CPC分类号： G01N21/6486 , G01N33/502 , G01N2800/44
摘要： This invention relates to multi-drug resistance (MDR) in cells, and the use of certain xanthene compounds for determining drug resistance in cells and the effect of test compounds on cell membrane transport by the membrane transporters MDR1, MRP and BCRP. Processes and kits for making these determinations and measuring these effects are described and provided.

5. 发明申请

US20100068752A1 Novel fluorescent dyes and compounds, methods and kits useful for identifying specific organelles and regions in cells of interest 有权
标题翻译：新型荧光染料和化合物，用于鉴定特定细胞器和感兴趣的细胞中的区域的方法和试剂盒
公开(公告)号：US20100068752A1
公开(公告)日：2010-03-18
申请号：US12586386
申请日：2009-09-21
申请人： Praveen Pande , Hilary J. Cox , Yuejun Xiang , Wayne Forrest Patton
发明人： Praveen Pande , Hilary J. Cox , Yuejun Xiang , Wayne Forrest Patton
IPC分类号： C12Q1/02 , C07F9/60 , C07F9/6533 , C07F9/6509 , C07D401/10 , C07D215/04
CPC分类号： G01N21/6486 , B82Y30/00 , C07D215/12 , C07D215/14 , C07D401/14 , C07D405/14 , C07D417/06 , C07D417/14 , C09B23/145 , G01N33/533 , G01N33/582 , Y10T436/16
摘要： The present invention provides dyes and labeled reagents that may be used in the detection or quantification of desirable target molecules, such as proteins, nucleic acids and cellular organelles. Dyes are provided that may be used free in solution where the binding of the dye to the target molecule provides signal generation. Dyes provided in this invention can comprise reactive groups that may be used to attach the dyes to probes that will bind to desirable target molecules. The novel dyes of the present invention have been substituted with specific groups to provide beneficial properties.
摘要翻译：本发明提供可用于检测或定量所需靶分子如蛋白质，核酸和细胞器的染料和标记试剂。提供可以在染料与靶分子的结合提供信号产生的溶液中游离的染料。在本发明中提供的染料可以包含反应性基团，其可以用于将染料连接到将与所需靶分子结合的探针上。本发明的新型染料已经被特定的基团取代以提供有益的性质。

6. 发明授权

US09250249B2 Autophagy and phospholipidosis pathway assays 有权
标题翻译：自噬和磷脂通路测定
公开(公告)号：US09250249B2
公开(公告)日：2016-02-02
申请号：US12584958
申请日：2009-09-14
申请人： Wayne Forrest Patton , Jack Coleman , Yuejun Xiang , Praveen Pande , Zaiguo Li
发明人： Wayne Forrest Patton , Jack Coleman , Yuejun Xiang , Praveen Pande , Zaiguo Li
IPC分类号： G01N33/68 , G01N33/50 , G01N33/58 , G01N33/94
CPC分类号： G01N33/6893 , G01N33/5076 , G01N33/582 , G01N33/94 , G01N2500/10 , G01N2800/02 , G01N2800/04 , G01N2800/52
摘要： Provided are assays useful for detecting and monitoring autophagy and phospholipidosis, including the progression of lysosomal storage diseases. Drugs and treatments for lysosomal storage diseases can be monitored for effectiveness in lysosomal storage disease conditions. Drug candidates and suspected toxic agents can also be screened for toxicity to cells, tissues and organs. Also provided are methods for distinguishing between phospholipidosis activators and autophagy pathway perturbation agents.
摘要翻译：提供用于检测和监测自噬和磷脂病的测定，包括溶酶体贮积病的进展。可以监测溶酶体储存疾病的药物和治疗溶酶体贮积病条件的有效性。药物候选人和疑似毒素也可以筛选对细胞，组织和器官的毒性。还提供了区分磷脂肪激活剂和自噬途径扰动剂的方法。

7. 发明申请

US20130137112A1 DYES FOR ANALYSIS OF PROTEIN AGGREGATION 审中-公开
标题翻译：用于分析蛋白质聚集的染料
公开(公告)号：US20130137112A1
公开(公告)日：2013-05-30
申请号：US13510976
申请日：2010-11-30
申请人： Wayne Forrest Patton , Sergiy M. Yarmoluk , Praveen Pande , Vladyslava Kovalska , Lijun Dai , Kateryna Volkova , Jack Coleman , Mykhaylo Losytskyy , Anthony Ludlam , Anatoliy Balanda , Dee Shen
发明人： Wayne Forrest Patton , Sergiy M. Yarmoluk , Praveen Pande , Vladyslava Kovalska , Lijun Dai , Kateryna Volkova , Jack Coleman , Mykhaylo Losytskyy , Anthony Ludlam , Anatoliy Balanda , Dee Shen
IPC分类号： C07D401/12
摘要： Provided are dyes and compositions which are useful in a number of applications, such as the detection and monitoring protein aggregation, kinetic studies of protein aggregation, neurofibrillary plaques analysis, evaluation of protein formulation stability, and analysis of molecular chaperone activity.
摘要翻译：提供可用于许多应用的染料和组合物，例如检测和监测蛋白质聚集，蛋白质聚集的动力学研究，神经原纤维斑块分析，蛋白质制剂稳定性的评估和分子伴侣活性的分析。

8. 发明授权

US08362250B2 Fluorescent dyes and compounds, methods and kits useful for identifying specific organelles and regions in cells of interest 有权
标题翻译：荧光染料和化合物，用于鉴定特定细胞器和感兴趣的细胞中的区域的方法和试剂盒
公开(公告)号：US08362250B2
公开(公告)日：2013-01-29
申请号：US12586386
申请日：2009-09-21
申请人： Praveen Pande , Hilary J. Cox , Yuejun Xiang , Wayne Forrest Patton
发明人： Praveen Pande , Hilary J. Cox , Yuejun Xiang , Wayne Forrest Patton
IPC分类号： C07F9/28 , C12Q1/00
CPC分类号： G01N21/6486 , B82Y30/00 , C07D215/12 , C07D215/14 , C07D401/14 , C07D405/14 , C07D417/06 , C07D417/14 , C09B23/145 , G01N33/533 , G01N33/582 , Y10T436/16
摘要： The present invention provides dyes and labeled reagents that may be used in the detection or quantification of desirable target molecules, such as proteins, nucleic acids and cellular organelles. Dyes are provided that may be used free in solution where the binding of the dye to the target molecule provides signal generation. Dyes provided in this invention can comprise reactive groups that may be used to attach the dyes to probes that will bind to desirable target molecules. The novel dyes of the present invention have been substituted with specific groups to provide beneficial properties.
摘要翻译：本发明提供可用于检测或定量所需靶分子如蛋白质，核酸和细胞器的染料和标记试剂。提供可以在染料与靶分子的结合提供信号产生的溶液中游离的染料。在本发明中提供的染料可以包含反应性基团，其可以用于将染料连接到将与所需靶分子结合的探针上。本发明的新型染料已经被特定的基团取代以提供有益的性质。

9. 发明申请

US20120183948A1 Compounds and methods for detection of enzymes that remove formyl, succinyl, methyl succinyl or myristoyl groups from epsilon-amino lysine moieties 有权
标题翻译：用于检测从ε-氨基赖氨酸部分去除甲酰基，琥珀酰基，甲基琥珀酰基或肉豆蔻酰基的酶的化合物和方法
公开(公告)号：US20120183948A1
公开(公告)日：2012-07-19
申请号：US12930693
申请日：2011-01-13
申请人： Konrad T. Howitz , Zhongda Zhang , Anne Kisielewski , Elizabeth Dale , Wayne Forrest Patton
发明人： Konrad T. Howitz , Zhongda Zhang , Anne Kisielewski , Elizabeth Dale , Wayne Forrest Patton
IPC分类号： C12Q1/00 , C07D493/10 , C07D311/16
CPC分类号： C12Q1/34 , C07D311/16 , C07D493/10 , C12Y305/01098 , G01N2333/98
摘要： Provided is a compound that comprises the structure: where SIG is a signaling molecule and R3 is a formyl, a succinyl, a methyl succinyl, or a myristoyl. Also provided is a kit is provided that comprises the above compound, with instructions for determining the presence of the enzyme. Additionally, a method is provided for determining whether a sample has an enzyme that removes a succinyl, a methyl succinyl, a formyl, or a myristoyl moiety from an ε-amino of a lysine. Also provided is a method of determining whether a molecule inhibits an enzyme that removes a succinyl, a methyl succinyl, a formyl, or a myristoyl moiety from an ε-amino of a lysine.
摘要翻译：提供了包含以下结构的化合物：其中SIG是信号分子，R3是甲酰基，琥珀酰基，甲基琥珀酰基或肉豆蔻酰基。还提供了包含上述化合物的试剂盒，其具有用于确定酶的存在的说明书。此外，提供了一种用于确定样品是否具有从赖氨酸的“ - ” - 氨基除去琥珀酰基，甲基琥珀酰基，甲酰基或肉豆蔻酰部分的酶的方法。还提供了确定分子是否抑制从赖氨酸的“ - ” - 氨基除去琥珀酰基，甲基琥珀酰基，甲酰基或肉豆蔻酰部分的酶的方法。

10. 发明申请

US20120115169A1 HYDROXYCHOLESTEROL IMMUNOASSAY 有权
标题翻译：羟吗啉酮免疫
公开(公告)号：US20120115169A1
公开(公告)日：2012-05-10
申请号：US12927206
申请日：2010-11-09
申请人： Michael C. Mullenix , Robert Elliot Zipkin , Jeffrey Kroll Adams , Wayne Forrest Patton , James J. Donegan
发明人： Michael C. Mullenix , Robert Elliot Zipkin , Jeffrey Kroll Adams , Wayne Forrest Patton , James J. Donegan
IPC分类号： G01N33/53 , C07J9/00 , C07K14/00 , C07J43/00 , G01N33/566 , C07K16/44
CPC分类号： G01N33/92 , C07J9/00 , C07J43/003 , C07K16/18 , C07K16/44 , C07K2317/21 , G01N33/5308 , G01N33/56966 , G01N2800/2835 , G01N2800/285 , G01N2800/52
摘要： Provided is a derivative of 22-hydroxycholesterol, 24S-hydroxycholesterol, 25-hydroxycholesterol, 26-hydroxycholesterol or 27-hydroxycholesterol. Also provided is a protein conjugated to the above derivative. Further provided is an antibody composition comprising antibodies that specifically bind to 22-hydroxycholesterol, 24S-hydroxycholesterol, 25-hydroxycholesterol, 26-hydroxycholesterol or 27-hydroxycholesterol. Additionally, a method of making antibodies that specifically bind to 22-hydroxycholesterol, 24S-hydroxycholesterol, 25-hydroxycholesterol, 26-hydroxycholesterol or 27-hydroxycholesterol is provided. Also, a method of assaying for 22-hydroxycholesterol, 24S-hydroxycholesterol, 25-hydroxycholesterol, 26-hydroxycholesterol or 27-hydroxycholesterol is provided. Additionally provided is a kit for detecting 22-hydroxycholesterol, 24S-hydroxycholesterol, 25-hydroxycholesterol, 26-hydroxycholesterol or 27-hydroxycholesterol. A method of detecting an enzyme or enzymes utilized in phase II drug metabolism is also provided. Also, a method of detecting an enzyme that synthesizes 22-hydroxycholesterol, 24S-hydroxycholesterol, 25-hydroxycholesterol, 26-hydroxycholesterol or 27-hydroxycholesterol is provided. Further provided is a method of evaluating progression of multiple sclerosis in a patient. Also provided is a method of determining whether a treatment for multiple sclerosis in a patient is effective. Further, a method of evaluating progression of Huntington's disease in a patient is provided. Additionally provided is a method of determining whether a treatment for Huntington's disease in a patient is effective.
摘要翻译：提供了22-羟基胆固醇，24S-羟基胆固醇，25-羟基胆固醇，26-羟基胆固醇或27-羟基胆固醇的衍生物。还提供了与上述衍生物结合的蛋白质。进一步提供了包含特异性结合22-羟基胆固醇，24S-羟基胆固醇，25-羟基胆固醇，26-羟基胆固醇或27-羟基胆固醇的抗体的抗体组合物。此外，提供了制备特异性结合22-羟基胆固醇，24S-羟基胆固醇，25-羟基胆固醇，26-羟基胆固醇或27-羟基胆固醇的抗体的方法。此外，提供了测定22-羟基胆固醇，24S-羟基胆固醇，25-羟基胆固醇，26-羟基胆固醇或27-羟基胆固醇的方法。另外提供了用于检测22-羟基胆固醇，24S-羟基胆固醇，25-羟基胆固醇，26-羟基胆固醇或27-羟基胆固醇的试剂盒。还提供了检测在II期药物代谢中使用的酶或酶的方法。此外，提供了检测合成22-羟基胆固醇，24S-羟基胆固醇，25-羟基胆固醇，26-羟基胆固醇或27-羟基胆固醇的酶的方法。进一步提供评估患者多发性硬化症进展的方法。还提供了确定患者多发性硬化症的治疗是否有效的方法。此外，提供了评价患者中亨廷顿病进展的方法。另外提供了一种确定患者亨廷顿氏病的治疗是否有效的方法。

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