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1. 发明授权

US11466297B2 Methods of oil production in microorganisms 有权
公开(公告)号：US11466297B2
公开(公告)日：2022-10-11
申请号：US17395459
申请日：2021-08-06
申请人： MARA RENEWABLES CORPORATION
发明人： Zhiyong Sun , Roberto E. Armenta , Mercia Valentine
IPC分类号： C12P7/6427 , C12P7/6463 , C12P7/6472
摘要： Provided herein are methods for producing one or more polyunsaturated fatty acids. The methods include the steps of providing a microorganism capable of producing polyunsaturated fatty acids, providing a medium comprising a high concentration of one or more carbon sources, low pH, or both, and culturing the microorganism in the medium under sufficient conditions to produce the one or more polyunsaturated fatty acids. Also provided are methods of culturing one or more microorganisms. The methods include culturing the microorganisms in a medium comprising a first amount of one or more carbon sources at a first concentration level, monitoring a carbon source concentration until the carbon source concentration is reduced below the first concentration level, and adding to the medium a second amount of one or more carbon sources to increase the carbon source concentration to a second concentration level.

2. 发明申请

US20210388312A1 ADAPTATION AND PROCESS OPTIMIZATION OF MICROORGANISMS FOR GROWTH IN HEMICELLULOSIC DERIVED CARBOHYDRATES 有权
公开(公告)号：US20210388312A1
公开(公告)日：2021-12-16
申请号：US17286449
申请日：2019-10-21
申请人： MARA RENEWABLES CORPORATION
发明人： Kimberly Hyson , Nathalia Florez , Denise Muise , Alexandra Merkx-Jacques , Dorothy Dennis , David Woodhall , Zachary Sun , Roberto E. Armenta
IPC分类号： C12N1/22 , C12N1/36 , C12N9/12 , C12N9/90
摘要： Provided herein are methods of making microorganisms modified for increased xylose consumption as compared to unmodified microorganisms. The methods include providing xylose-consuming microorganisms comprising two or more copies of a nucleic acid sequence encoding xylose isomerase and two or more copies of a nucleic acid sequence encoding a xylose kinase, culturing the microorganisms in medium containing xylose and harvesting a portion of the microorganisms. These steps are repeated multiple times. The microorganisms are then isolated. The isolated microorganisms have increased xylose consumption rates compared to control xylose-consuming microorganisms. Also provided are a population of microorganisms made by the provided methods. Methods of culturing the population of microorganisms and methods of reducing xylitol production in cultures comprising the population of microorganisms are provided.

3. 发明授权

US10851395B2 Method of making lipids with improved cold flow properties 有权
公开(公告)号：US10851395B2
公开(公告)日：2020-12-01
申请号：US15178890
申请日：2016-06-10
申请人： MARA RENEWABLES CORPORATION
发明人： Zhiyong Sun , Alan Sothern , Kevin Berryman , Mercia Valentine , Michael Milway , Laura Purdue , Roberto E. Armenta
IPC分类号： C12P7/64
摘要： Provided herein are methods of producing oils with reduced saturated fatty acids. The methods include culturing oil-producing microorganisms in a fermentation medium in the presence of one or more antifoaming agents under a controlled carbon consumption rate, wherein the culturing produces oils comprising fatty acids and wherein less than 35% of the fatty acids in the oil are saturated fatty acids.

4. 发明授权

US10745642B2 Methods of recovering oil from microorganisms 有权
公开(公告)号：US10745642B2
公开(公告)日：2020-08-18
申请号：US15659300
申请日：2017-07-25
申请人： MARA Renewables Corporation
发明人： Dorothy A. Dennis , Roberto E. Armenta
IPC分类号： C11B1/10 , C12P7/64
摘要： Provided herein are methods of recovering oil from microorganisms. The methods are useful, for example, in obtaining nutritional oils and/or lipid biofuels. The methods of recovering oil described herein include contacting a population of microorganisms with one or more enzymes under conditions that cause disruption of the microorganisms, concentrating the disrupted microorganisms, and extracting lipids from the disrupted microorganisms at high temperature in the presence of a salt and in the absence of solvent.

5. 发明申请

US20150337345A1 METHODS OF OIL PRODUCTION IN MICROORGANISMS 审中-公开
标题翻译：微生物油生产方法
公开(公告)号：US20150337345A1
公开(公告)日：2015-11-26
申请号：US14719183
申请日：2015-05-21
申请人： MARA Renewables Corporation
发明人： Zhiyong Sun , Roberto E. Armenta , Mercia Valentine
IPC分类号： C12P7/64
CPC分类号： C12P7/6427 , C12P7/6463 , C12P7/6472
摘要： Provided herein are methods for producing one or more polyunsaturated fatty acids. The methods include the steps of providing a microorganism capable of producing polyunsaturated fatty acids, providing a medium comprising a high concentration of one or more carbon sources, low pH, or both, and culturing the microorganism in the medium under sufficient conditions to produce the one or more polyunsaturated fatty acids. Also provided are methods of culturing one or more microorganisms. The methods include culturing the microorganisms in a medium comprising a first amount of one or more carbon sources at a first concentration level, monitoring a carbon source concentration until the carbon source concentration is reduced below the first concentration level, and adding to the medium a second amount of one or more carbon sources to increase the carbon source concentration to a second concentration level.
摘要翻译：本文提供了制备一种或多种多不饱和脂肪酸的方法。所述方法包括提供能够产生多不饱和脂肪酸的微生物，提供包含高浓度一种或多种碳源，低pH或两者的培养基的步骤，以及在足够条件下在培养基中培养微生物以产生一种或更多的多不饱和脂肪酸。还提供培养一种或多种微生物的方法。所述方法包括在包含第一量的一种或多种碳源的培养基中培养微生物，所述培养基以第一浓度水平监测碳源浓度，直到碳源浓度降低到低于第一浓度水平，并向培养基中加入第二一个或多个碳源的量将碳源浓度增加到第二浓度水平。

6. 发明申请

US20200332338A1 SEMI-CONTINUOUS CULTURE METHODS 审中-公开
公开(公告)号：US20200332338A1
公开(公告)日：2020-10-22
申请号：US16864128
申请日：2020-04-30
申请人： Mara Renewables Corporation
发明人： Zhiyong Sun , Michael Milway , Kevin Berryman , Mercia Valentine , Roberto E. Armenta , Laura Purdue
IPC分类号： C12Q1/06 , C12P7/64 , C12N1/12 , C12N1/14 , C12N1/04
摘要： Provided herein are methods of culturing a microorganism. The methods include providing a container comprising one or more microorganisms in a medium, which has a first carbon to nitrogen ratio; culturing the microorganisms until the culture reaches a threshold indicator; harvesting a portion of the culture while maintaining the majority of the culture in the container; and adding fresh medium comprising a second carbon to nitrogen ratio to the container with the majority of the culture comprising the microorganisms.

7. 发明申请

US20200325465A1 ENHANCING MICROBIAL METABOLISM OF C5 ORGANIC CARBON 审中-公开
公开(公告)号：US20200325465A1
公开(公告)日：2020-10-15
申请号：US16864098
申请日：2020-04-30
申请人： MARA Renewables Corporation
发明人： Alexandra Merkx-Jacques , David Woodhall , Mark Scaife , Roberto E. Armenta , Denise Muise , Holly Rasmussen , Jeremy Benjamin
IPC分类号： C12N9/92 , C12N9/12 , C07K14/40 , C12P7/64 , C07K14/38 , C12N1/12 , C12N1/10
摘要： Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganisms include at least one nucleic acid sequence encoding a xylulose kinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganisms are capable of growing on xylose as a carbon source.

8. 发明授权

US10676709B2 Flowable microbial oil 有权
公开(公告)号：US10676709B2
公开(公告)日：2020-06-09
申请号：US15655287
申请日：2017-07-20
申请人： Mara Renewables Corporation
发明人： Xuan Jiang , Dorothy Dennis , Roberto E. Armenta
IPC分类号： C12N1/26 , C11B1/02 , C12N1/12 , C07C7/10 , C11B1/10
摘要： Provided herein is a method for obtaining flowable oil comprising the steps of providing a population of oil-producing microorganisms; recovering oil from the microorganisms, wherein the oil is at a first temperature; reducing the first temperature over a first period of time to a second temperature; and applying mechanical energy to the oil during the first period of time thereby producing the flowable oil.

9. 发明申请

US20180208954A1 METHOD OF MAKING LIPIDS WITH IMPROVED COLD FLOW PROPERTIES 审中-公开
公开(公告)号：US20180208954A1
公开(公告)日：2018-07-26
申请号：US15926680
申请日：2018-03-20
申请人： MARA RENEWABLES CORPORATION
发明人： Zhiyong Sun , Alan Sothern , Kevin Berryman , Mercia Valentine , Michael Milway , Laura Purdue , Roberto E. Armenta
IPC分类号： C12P7/64
CPC分类号： C12P7/6427 , C12P7/6463
摘要： Provided herein are methods of producing oils with reduced saturated fatty acids. The methods include culturing oil-producing microorganisms in a fermentation medium in the presence of one or more antifoaming agents under a controlled carbon consumption rate, wherein the culturing produces oils comprising fatty acids and wherein less than 35% of the fatty acids in the oil are saturated fatty acids.

10. 发明申请

US20180179560A1 METHODS FOR PRODUCING BIOMASS RICH IN DHA, PALMITIC ACID AND PROTEIN USING A EUKARYOTIC MICROORGANISM 审中-公开
公开(公告)号：US20180179560A1
公开(公告)日：2018-06-28
申请号：US15845697
申请日：2017-12-18
申请人： MARA RENEWABLES CORPORATION
发明人： Violeta Ugalde , Zachary Sun , Anthony Windust , Roberto E. Armenta
IPC分类号： C12P7/64 , C12P23/00 , C12P21/00 , C12R1/89 , C12N1/12 , A23L33/135 , A23K50/00 , A23K10/18 , A23K20/147
CPC分类号： C12P7/6427 , A23K10/18 , A23K20/147 , A23K20/158 , A23K50/00 , A23L33/115 , A23L33/135 , A23V2002/00 , C12N1/10 , C12N1/12 , C12N1/14 , C12P7/6409 , C12P7/6472 , C12P21/00 , C12P23/00 , C12R1/645 , C12R1/89
摘要： Provided herein are eukaryotic microorganisms having a simple lipid profile comprising long chain fatty acids (LCFAs). Also provided are compositions and cultures comprising the eukaryotic microorganisms as well as methods of using the eukaryotic microorganisms.

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