专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US4950613A Proteted chemiluminescent labels 失效
标题翻译：防护化学发光标签
公开(公告)号：US4950613A
公开(公告)日：1990-08-21
申请号：US160611
申请日：1988-02-26
申请人： Lyle J. Arnold, Jr. , Alexander A. Waldrop, III , Philip W. Hammond
发明人： Lyle J. Arnold, Jr. , Alexander A. Waldrop, III , Philip W. Hammond
IPC分类号： G01N33/532 , C07D215/48 , C07D215/50 , C07D219/04 , C07D221/12 , C12Q1/68 , G01N21/76 , G01N33/00 , G01N33/52 , G01N33/533 , G01N33/58
CPC分类号： G01N33/533 , G01N33/582
摘要： A method of preparing a labelled specific binding partner, such as a biological probe in the form of an antibody or oligonucleotide probe, using a protected label (the corresponding unprotected label being susceptible to inactivation, such as by hydrolysis to yield a non-chemiluminescent form of the label). The specific binding partner is linked to the label, and an adduct of the label is prepared using a protective adduct former which produces a protected label which is less susceptible to inactivation. Particularly preferred labels are the acridiniums and acridans, most preferably the former having the general structure: ##STR1## wherein the phenyl rings are optionally additionally substituted, R.sub.1 is preferably an alkyl, and R.sub.5 is an optionally substituted hydrocarbon, most preferably a phenyl moiety which is either linked to the specific binding partner, or capable of being linked thereto. Formation of the protected label is preferably an equilibrium reaction which is readily reversible, such as by dilution or oxidation of the protective adduct former. Labels useful in such a method are also disclosed.

2. 发明授权

US5599667A Polycationic supports and nucleic acid purification separation and hybridization 失效
标题翻译：用于核酸纯化，分离和杂交的聚阳离子载体
公开(公告)号：US5599667A
公开(公告)日：1997-02-04
申请号：US311289
申请日：1994-09-23
申请人： Lyle J. Arnold, Jr. , Norman C. Nelson , Mark A. Reynolds , Alexander A. Waldrop, III
发明人： Lyle J. Arnold, Jr. , Norman C. Nelson , Mark A. Reynolds , Alexander A. Waldrop, III
IPC分类号： C07H1/06 , C07H21/00 , C12Q1/68 , C12N15/10
CPC分类号： C07H21/00 , C07H1/06 , C12Q1/6806 , C12Q1/6834 , C12Q1/689
摘要： Described herein is the use of polycationic solid supports in the purification of nucleic acids from solutions containing contaminants. The nucleic acids non-covalently bind to the support without significant binding of contaminants permitting their separation from the contaminants. The bound nucleic acids can be recovered from the support. Also described is the use of the supports as a means to separate polynucleotides and hybrids thereof with a nucleotide probe from unhybridized probe. Assays for target nucleotide sequences are described which employ this separation procedure.
摘要翻译：本文描述的是使用聚阳离子固体载体从含有污染物的溶液中纯化核酸。核酸与载体非共价结合，而不会使污染物与污染物分离而明显结合污染物。结合的核酸可以从载体中回收。还描述了使用载体作为用来自未杂交的探针的核苷酸探针分离多核苷酸和其杂交体的手段。描述了使用这种分离方法的靶核苷酸序列的测定。

3. 发明授权

US4711955A Modified nucleotides and methods of preparing and using same 失效
标题翻译：修饰的核苷酸及其制备和使用方法
公开(公告)号：US4711955A
公开(公告)日：1987-12-08
申请号：US496915
申请日：1983-05-23
申请人： David C. Ward , Pennina R. Langer , Alexander A. Waldrop, III
发明人： David C. Ward , Pennina R. Langer , Alexander A. Waldrop, III
IPC分类号： C07H19/167 , C07H19/173 , C07H19/20 , C07H21/00 , C07H21/02 , C12P19/34 , C12Q1/18 , C12Q1/68 , C07H17/02 , C07H19/02 , C07H19/06
CPC分类号： C07H19/20 , C07H21/00 , C12P19/34 , C12Q1/18 , C12Q1/6804 , C12Q1/6816
摘要： Compounds having the structure: ##STR1## wherein B represents a purine, 7-deazapurine, or pyrimidine moiety covalently bonded to the C.sup.1' -position of the sugar moiety, provided that when B is purine or 7-deazapurine, it is attached at the N.sup.9 -position of the purine or 7-deazapurine and when B is pyrimidine, it is attached at the N.sup.1 -position;wherein A represents a moiety consisting of at least three carbon atoms which is capable of forming a detectable complex with a polypeptide when the compound is incorporated into a double-stranded ribonucleic acid, deoxyribonucleic acid duplex, or DNA-RNA hybrid;wherein the dotted line represents a chemical linkage joining B and A, provided that if B is purine, the linkage is attached to the 8-position of the purine, if B is 7-deazapurine, the linkage is attached to the 7-position of the deazapurine, and if B is pyrimidine, the linkage is attached to the 5-position of the pyrimidine andwherein each of x, y and z represents ##STR2## either directly, or when incorporated into oligo- and polynucleotides, provide probes which are widely useful.Applications include detection and localization of polynucleotide sequences in chromosomes, fixed cells, tissue sections, and cell extracts. Specific applications include chromosomal karyotyping, clinical diagnosis of nucleic acid-containing etiological agents, e.g. bacteria, viruses, or fungi, and diagnosis of genetic disorders.
摘要翻译：具有以下结构的化合物：其中B表示与糖部分的C1'位共价结合的嘌呤，7-脱氮嘌呤或嘧啶部分，条件是当B是嘌呤或7-脱氮嘌呤时，其连接在嘌呤或7-脱氮嘌呤的N9位，当B为嘧啶时，其连接在N1位; 其中A表示由至少三个碳原子组成的部分，当化合物掺入双链核糖核酸，脱氧核糖核酸双链体或DNA-RNA杂交体时，能够与多肽形成可检测的复合物; 其中虚线表示连接B和A的化学连接，条件是如果B是嘌呤，则如果B是7-脱氮嘌呤，则连接键连接到嘌呤的8-位，连接键连接到嘌呤的7-位脱氮嘌呤，如果B是嘧啶，则该连接键连接到嘧啶的5-位，并且x，y和z中的每一个直接或当并入寡核苷酸和多核苷酸时提供广泛有用的探针。应用包括染色体，固定细胞，组织切片和细胞提取物中多核苷酸序列的检测和定位。具体应用包括染色体核型分析，含核酸病原体的临床诊断。细菌，病毒或真菌，以及遗传疾病的诊断。

4. 发明授权

US5476928A Modified nucleotides and polynucleotides and complexes form therefrom 失效
标题翻译：由此形成修饰的核苷酸和多核苷酸和复合物
公开(公告)号：US5476928A
公开(公告)日：1995-12-19
申请号：US841910
申请日：1992-02-26
申请人： David C. Ward , Pennina R. Langer , Alexander A. Waldrop, III
发明人： David C. Ward , Pennina R. Langer , Alexander A. Waldrop, III
IPC分类号： C07H19/167 , C07H19/173 , C07H19/20 , C07H21/00 , C07H21/02 , C12P19/34 , C12Q1/18 , C12Q1/68
CPC分类号： C07H19/20 , C07H21/00 , C12P19/34 , C12Q1/18 , C12Q1/6804 , C12Q1/6816
摘要： Compounds having the structure: ##STR1## wherein B represents a purine, 7-deazapurine, or pyrimidine moiety covalently bonded to the C.sup.1' -position of the sugar moiety, provided that when B is purine or 7-deazapurine, it is attached at the N.sup.9 -position of the purine or 7-deazapurine and when B is pyrimidine, it is attached at the N.sup.1 -position;wherein A represents a moiety consisting of at least three carbon atoms which is capable of forming a detectable complex with a polypeptide when the compound is incorporated into a double-stranded ribonucleic acid, deoxyribonucleic acid duplex, or DNA-RNA hybrid;wherein the dotted line represents a chemical linkage joining B and A, provided that if B is purine, the linkage is attached to the 8-position of the purine, if B is 7-deazapurine, the linkage is attached to the 7-position of the deazapurine, and if B is pyrimidine, the linkage is attached to the 5-position of the pyrimidine; andwherein each of x, y and z represents ##STR2## either directly, or when incorporated into oligo- and polynucleotides, provide probes which are widely useful.Applications include detection and localization of polynucleotide sequences in chromosomes, fixed cells, tissue sections, and cell extracts. Specific applications include chromosomal karyotyping, clinical diagnosis of nucleic acid-containing etiological agents, e.g. bacteria, viruses, or fungi, and diagnosis of genetic disorders.
摘要翻译：具有以下结构的化合物：其中B表示与糖部分的C1'位共价结合的嘌呤，7-脱氮嘌呤或嘧啶部分，条件是当B是嘌呤或7-脱氮嘌呤时，其连接在嘌呤或7-脱氮嘌呤的N9位，当B为嘧啶时，其连接在N1位; 其中A表示由至少三个碳原子组成的部分，当化合物掺入双链核糖核酸，脱氧核糖核酸双链体或DNA-RNA杂交体时，能够与多肽形成可检测的复合物; 其中虚线表示连接B和A的化学连接，条件是如果B是嘌呤，则如果B是7-脱氮嘌呤，则连接键连接到嘌呤的8-位，连接键连接到嘌呤的7-位脱氮嘌呤，如果B是嘧啶，则连接键连接到嘧啶的5-位; 并且其中x，y和z中的每一个直接地或当并入寡核苷酸和多核苷酸时提供广泛有用的探针。应用包括染色体，固定细胞，组织切片和细胞提取物中多核苷酸序列的检测和定位。具体应用包括染色体核型分析，含核酸病原体的临床诊断。细菌，病毒或真菌，以及遗传疾病的诊断。

5. 发明授权

US5449767A Modified polynucleotides and methods of preparing same 失效
标题翻译：改性多核苷酸及其制备方法
公开(公告)号：US5449767A
公开(公告)日：1995-09-12
申请号：US886660
申请日：1992-05-20
申请人： David C. Ward , Pennina R. Langer , Alexander A. Waldrop, III
发明人： David C. Ward , Pennina R. Langer , Alexander A. Waldrop, III
IPC分类号： C07H19/167 , C07H19/173 , C07H19/20 , C07H21/00 , C07H21/02 , C12P19/34 , C12Q1/18 , C12Q1/68
CPC分类号： C07H19/20 , C07H21/00 , C12P19/34 , C12Q1/18 , C12Q1/6804 , C12Q1/6816
摘要： Compounds having the structure: ##STR1## wherein B represents 7-deazapurine, or pyrimidine moiety covalently bonded to the C.sup.1 '-position of the sugar moiety, provided that when B is 7-deazapurine, it is attached at the N.sup.9 -position of the 7-deazapurine and when B is pyrimidine, it is attached at the N.sup.1 -position;wherein A represents a moiety consisting of at least three carbon atoms which is capable of forming a detectable complex with a polypeptide when the compound is incorporated into a double-stranded ribonucleic acid, deoxyribonucleic acid duplex, or DNA-RNA hybrid;wherein the dotted line represents a chemical linkage joining B and A, provided that if B is 7-deazapurine, the linkage is attached to the 7-position of the deazapurine, and if B is pyrimidine, the linkage is attached to the 5-position of the pyrimidine; andwherein each of x, y and z represents ##STR2## either directly, or when incorporated into oligo- and polynucleotides, provide probes which are widely useful.
摘要翻译：具有以下结构的化合物：其中B代表7-脱氮嘌呤或与糖部分的C1'位共价结合的嘧啶部分，条件是当B为7-脱氮嘌呤时，其连接在 7-脱氮嘌呤，当B为嘧啶时，其连接在N1位; 其中A表示由至少三个碳原子组成的部分，当化合物掺入双链核糖核酸，脱氧核糖核酸双链体或DNA-RNA杂交体时，能够与多肽形成可检测的复合物; 其中虚线表示连接B和A的化学键，条件是如果B是7-脱氮嘌呤，则连接键连接到脱氮嘌呤的7-位，如果B是嘧啶，则连接键连接到5-位的嘧啶; 并且其中x，y和z中的每一个直接表示“IMAGE”或“IMAGE”，或者当并入寡核苷酸和多核苷酸时，提供广泛有用的探针。

6. 发明授权

US5328824A Methods of using labeled nucleotides 失效
标题翻译：使用标记核苷酸的方法
公开(公告)号：US5328824A
公开(公告)日：1994-07-12
申请号：US130070
申请日：1987-12-08
申请人： David C. Ward , Pennina R. Langer , Alexander A. Waldrop, III
发明人： David C. Ward , Pennina R. Langer , Alexander A. Waldrop, III
IPC分类号： C07H19/167 , C07H19/173 , C07H19/20 , C07H21/00 , C07H21/02 , C12P19/34 , C12Q1/18 , C12Q1/68
CPC分类号： C07H19/20 , C07H21/00 , C12P19/34 , C12Q1/18 , C12Q1/6804 , C12Q1/6816
摘要： Compounds having the structure: ##STR1## wherein B represents a purine, 7-deazapurine, or pyrimidine moiety covalently bonded to the C.sup.1' -position of the sugar moiety, provided that when B is purine or 7-deazapurine, it is attached at the N.sup.9 -position of the purine or 7-deazapurine and when B is pyrimidine, it is attached at the N.sup.1 -position;wherein A represents a moiety consisting of at least three carbon atoms which is capable of forming a detectable complex with a polypeptide when the compound is incorporated into a double-stranded ribonucleic acid, deoxyribonucleic acid duplex, or DNA-RNA hybrid;wherein the dotted line represents a chemical linkage joining B and A, provided that if B is purine, the linkage is attached to the 8-position of the purine, if B is 7-deazapurine, the linkage is attached to the 7-position of the deazapurine, and if B is pyrimidine, the linkage is attached to the 5-position of the pyrimidine; andwherein each of x, y and z represents ##STR2## either directly, or when incorporated into oligo- and polynucleotides, provide probes which are widely useful.Applications include detection and localization of polynucleotide sequences in chromosomes, fixed cells, tissue sections, and cell extracts. Specific applications include chromosomal karyotyping, clinical diagnosis of nucleic acid-containing etiological agents, e.g. bacteria, viruses, or fungi, and diagnosis of genetic disorders.
摘要翻译：具有以下结构的化合物：其中B表示与糖部分的C1'位共价结合的嘌呤，7-脱氮嘌呤或嘧啶部分，条件是当B是嘌呤或7-脱氮嘌呤时，其连接在嘌呤或7-脱氮嘌呤的N9位，当B为嘧啶时，其连接在N1位; 其中A表示由至少三个碳原子组成的部分，当化合物掺入双链核糖核酸，脱氧核糖核酸双链体或DNA-RNA杂交体时，能够与多肽形成可检测的复合物; 其中虚线表示连接B和A的化学连接，条件是如果B是嘌呤，则如果B是7-脱氮嘌呤，则连接键连接到嘌呤的8-位，连接键连接到嘌呤的7-位脱氮嘌呤，如果B是嘧啶，则连接键连接到嘧啶的5-位; 并且其中x，y和z中的每一个直接地或当并入寡核苷酸和多核苷酸时提供广泛有用的探针。应用包括染色体，固定细胞，组织切片和细胞提取物中多核苷酸序列的检测和定位。具体应用包括染色体核型分析，含核酸病原体的临床诊断。细菌，病毒或真菌，以及遗传疾病的诊断。

7. 发明授权

US07718576B2 Peroxide-based chemiluminescent assays and chemiluminescent compounds used therein 失效
标题翻译：其中使用过氧化物的化学发光测定和化学发光化合物
公开(公告)号：US07718576B2
公开(公告)日：2010-05-18
申请号：US10457385
申请日：2003-06-10
申请人： Alexander A. Waldrop, III , Calvin P. H. Vary
发明人： Alexander A. Waldrop, III , Calvin P. H. Vary
IPC分类号： C40B30/00
CPC分类号： C12Q1/28 , C07D219/04
摘要： A compound is provided having the formula: where C* is an sp2 coordinated carbon atom; A is defined by the formula wherein R12, R13, R14, R15, R16, R17, R18 and R19 are independently selected from the group consisting of hydrogen, alkyl, alkoxy, aryl, alkylaryl, heteroaryl, heteroalkoxy, aldehyde, keto, amino, nitro, halo, sulfate, sulfonyl, carboxy, carboxyester, phosphate or phosphoester, each of which may be substituted or unsubstituted; Z is a moiety that is covalently bonded to C* selected from the group consisting of O, S, N—R1, and +N—R1R2 where R1 and R2 are selected from hydrogen, alkyl, alkoxy, aryl, alkylaryl, heteroaryl, or heteroalkoxy moieties, each of which may be substituted or unsubstituted; and Q is a suitable leaving group yields a compound which is capable of exhibiting a chemiluminescent reaction in the presence of a peroxide or peroxide-like compound under aqueous or mixed aqueous-organic conditions.
摘要翻译：提供具有下式的化合物：其中C *是sp2配位碳原子; A由下式定义：其中R12，R13，R14，R15，R16，R17，R18和R19独立地选自氢，烷基，烷氧基，芳基，烷基芳基，杂芳基，杂烷氧基，醛，酮，氨基，硝基，卤素，硫酸根，磺酰基，羧基，羧酸酯，磷酸酯或磷酯，它们各自可以是取代或未取代的; Z是与C *共价连接的部分，其选自O，S，N-R 1和+ N-R 1 R 2，其中R 1和R 2选自氢，烷基，烷氧基，芳基，烷基芳基，杂芳基或杂烷氧基部分，其各自可以是取代或未取代的; 并且Q是合适的离去基团，得到在水溶液或混合水 - 有机条件下能够在过氧化物或过氧化物类化合物存在下显示化学发光反应的化合物。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式