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1. 发明授权

US07718576B2 Peroxide-based chemiluminescent assays and chemiluminescent compounds used therein 失效
标题翻译：其中使用过氧化物的化学发光测定和化学发光化合物
公开(公告)号：US07718576B2
公开(公告)日：2010-05-18
申请号：US10457385
申请日：2003-06-10
申请人： Alexander A. Waldrop, III , Calvin P. H. Vary
发明人： Alexander A. Waldrop, III , Calvin P. H. Vary
IPC分类号： C40B30/00
CPC分类号： C12Q1/28 , C07D219/04
摘要： A compound is provided having the formula: where C* is an sp2 coordinated carbon atom; A is defined by the formula wherein R12, R13, R14, R15, R16, R17, R18 and R19 are independently selected from the group consisting of hydrogen, alkyl, alkoxy, aryl, alkylaryl, heteroaryl, heteroalkoxy, aldehyde, keto, amino, nitro, halo, sulfate, sulfonyl, carboxy, carboxyester, phosphate or phosphoester, each of which may be substituted or unsubstituted; Z is a moiety that is covalently bonded to C* selected from the group consisting of O, S, N—R1, and +N—R1R2 where R1 and R2 are selected from hydrogen, alkyl, alkoxy, aryl, alkylaryl, heteroaryl, or heteroalkoxy moieties, each of which may be substituted or unsubstituted; and Q is a suitable leaving group yields a compound which is capable of exhibiting a chemiluminescent reaction in the presence of a peroxide or peroxide-like compound under aqueous or mixed aqueous-organic conditions.
摘要翻译：提供具有下式的化合物：其中C *是sp2配位碳原子; A由下式定义：其中R12，R13，R14，R15，R16，R17，R18和R19独立地选自氢，烷基，烷氧基，芳基，烷基芳基，杂芳基，杂烷氧基，醛，酮，氨基，硝基，卤素，硫酸根，磺酰基，羧基，羧酸酯，磷酸酯或磷酯，它们各自可以是取代或未取代的; Z是与C *共价连接的部分，其选自O，S，N-R 1和+ N-R 1 R 2，其中R 1和R 2选自氢，烷基，烷氧基，芳基，烷基芳基，杂芳基或杂烷氧基部分，其各自可以是取代或未取代的; 并且Q是合适的离去基团，得到在水溶液或混合水 - 有机条件下能够在过氧化物或过氧化物类化合物存在下显示化学发光反应的化合物。

2. 发明授权

US5800984A Nucleic acid sequence detection by triple helix formation at primer site in amplification reactions 失效
标题翻译：核酸序列检测在扩增反应中引物位点处的三螺旋形成
公开(公告)号：US5800984A
公开(公告)日：1998-09-01
申请号：US294424
申请日：1994-08-23
申请人： Calvin P. H. Vary
发明人： Calvin P. H. Vary
IPC分类号： C12Q1/68 , C12Q1/70 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6839 , C12Q1/6853
摘要： Disclosed is a method for detecting a nucleic acid target sequence by formation of triple helix nucleic acid structures. The method may, but need not, involve amplifying the nucleic acid in vitro using cycles of denaturation and amplification to yield product duplexes, and detecting the product duplexes by hybridizing a third strand of nucleic acid to the product duplexes without denaturation. The triple helix-forming duplex sequences may be endogenous to the target sequence being detected, or they may be introduced in the probes used during amplification.
摘要翻译：公开了通过形成三重螺旋核酸结构来检测核酸靶序列的方法。该方法可以但不是必须涉及在体外使用变性和扩增循环来扩增核酸以产生产物双链体，以及通过将第三链核酸与产物双链体杂交而不变性来检测产物双链体。形成三螺旋的双链体序列可以是待检测的靶序列的内源性，或者可以将其引入扩增期间使用的探针。

3. 发明授权

US5994078A Stable encapsulated reference nucleic acid and method of making 失效
公开(公告)号：US5994078A
公开(公告)日：1999-11-30
申请号：US997522
申请日：1997-12-23
申请人： Clark A. Rundell , Calvin P. H. Vary
发明人： Clark A. Rundell , Calvin P. H. Vary
IPC分类号： C12Q1/68 , C12Q1/70 , C07H19/00 , C07H21/02
CPC分类号： C12Q1/6806
摘要： Stable reference nucleic acid for use in all steps of molecular screening and diagnostic assays and method of making. A desired nucleic acid sequence is amplified, ligated into an expression vector, and used to transform a vehicle. A cellular vehicle is subsequently killed without affecting the encapsulated nucleic acid. The vehicle membrane is stabilized under controlled conditions to a stability substantially matching that of a test cell membrane. The recovered nucleic acid is used as a standard or control in molecular diagnostic and genetic testing assays.

4. 发明授权

US4767699A Diagnostic reagent, kit and method employing polynucleotide displacement, separation, enzymatic cleavage and adenosine phosphate detection 失效
标题翻译：使用多核苷酸置换，分离，酶切和磷酸腺苷检测的诊断试剂，试剂盒和方法
公开(公告)号：US4767699A
公开(公告)日：1988-08-30
申请号：US729503
申请日：1985-05-02
申请人： Calvin P. H. Vary , Steven E. Diamond , Neil M. Wolfman
发明人： Calvin P. H. Vary , Steven E. Diamond , Neil M. Wolfman
IPC分类号： G01N33/50 , C12Q1/66 , C12Q1/68 , G01N33/53 , G01N33/566
CPC分类号： C12Q1/6816 , C12Q1/66 , C12Q1/6823 , Y10S435/81 , Y10S436/808
摘要： A reagent complex is disclosed containing (1) a probe polynucleotide having a target binding region complementary to a target nucleotide sequence and (2) a signal strand polynucleotide bound by base pairing to a portion of the target binding region. The signal strand is displaced from the reagent complex by the target nucleotide sequence and separated from intact reagent complexes. A digestable ribonucleotide segment of the displaced signal strand, such as a 3' , terminal segment, is digested to ribonucleotide phosphates, which are phosphorylated to ATP. The ribonucleotide phosphates or ATP are detected.
摘要翻译：公开了一种试剂复合物，其包含（1）具有与靶核苷酸序列互补的靶结合区的探针多核苷酸，和（2）通过碱基配对与目标结合区的一部分结合的信号链多核苷酸。信号链通过靶核苷酸序列从试剂复合体移位，并与完整的试剂配合物分离。被置换的信号链的可消化的核糖核苷酸片段如3'+ 0末端片段被消化成核糖核苷酸磷酸酯，其被磷酸化成ATP。检测到核糖核苷酸磷酸酯或ATP。

5. 发明授权

US6074825A Stable encapsulated reference nucleic acid and method of making 失效
标题翻译：稳定包封的参考核酸及其制备方法
公开(公告)号：US6074825A
公开(公告)日：2000-06-13
申请号：US905124
申请日：1997-07-31
申请人： Clark A. Rundell , Calvin P. H. Vary
发明人： Clark A. Rundell , Calvin P. H. Vary
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806
摘要： Stable reference nucleic acid for use in all steps of molecular screening and diagnostic assays and method of making. A desired nucleic acid sequence is amplified, ligated into an expression vector, and used to transform a vehicle. A cellular vehicle is subsequently killed without affecting the encapsulated nucleic acid. The vehicle membrane is stabilized under controlled conditions to a stability substantially matching that of a test cell membrane. The recovered nucleic acid is used as a standard or control in molecular diagnostic and genetic testing assays.
摘要翻译：稳定的参考核酸用于分子筛选和诊断测定的所有步骤和制备方法。将期望的核酸序列扩增，连接到表达载体中，并用于转化载体。随后杀死细胞媒介物而不影响包封的核酸。载体膜在受控条件下稳定至基本上与测试细胞膜的稳定性相当的稳定性。回收的核酸用作分子诊断和基因测试测定中的标准或对照。

6. 发明授权

US4735897A Method and kit for detecting polyriboadenosine segments and messenger RNA 失效
标题翻译：检测多聚核苷酸片段和信使RNA的方法和试剂盒
公开(公告)号：US4735897A
公开(公告)日：1988-04-05
申请号：US729502
申请日：1985-05-02
申请人： Calvin P. H. Vary , Steven E. Diamond , Neil M. Wolfman , Astrid P. Koudelka
发明人： Calvin P. H. Vary , Steven E. Diamond , Neil M. Wolfman , Astrid P. Koudelka
IPC分类号： G01N33/50 , C12N15/00 , C12N15/09 , C12Q1/48 , C12Q1/68 , C12Q1/42 , C12Q1/50 , G01N33/53
CPC分类号： C12Q1/6816 , C12Q1/48 , C12Q1/68 , C12Q1/6823 , Y10S435/81 , Y10S435/975
摘要： RNA such as messenger RNA is digested to nucleotide phosphates including AMP or ADP. The ATP or a byproduct of the phosphorylation, e.g., pyruvate, is detected. Exemplary enzymes used (with appropriate co-reactants and co-factors) are: (1) polynucleotide phosphorylase, pyruvate kinase and luciferase, or (2) phosphodiesterase (or RNase), myokinase, pyruvate kinase and luciferase. The phosphorylation to ATP (e.g., with pyruvate kinase) is preferably coupled with the previous (reversible) enzymatic step.
摘要翻译：诸如信使RNA的RNA被消化成包括AMP或ADP的核苷酸磷酸酯。检测ATP或磷酸化的副产物，例如丙酮酸。使用的示例性酶（具有合适的共反应物和辅因子）是：（1）多核苷酸磷酸化酶，丙酮酸激酶和荧光素酶，或（2）磷酸二酯酶（或RNA酶），肌激酶，丙酮酸激酶和荧光素酶。磷酸化至ATP（例如，与丙酮酸激酶）的磷酸化优选与先前的（可逆的）酶促步骤偶联。

7. 发明授权

US4851331A Method and kit for polynucleotide assay including primer-dependant DNA polymerase 失效
标题翻译：用于多核苷酸测定的方法和试剂盒，包括引物依赖性DNA聚合酶
公开(公告)号：US4851331A
公开(公告)日：1989-07-25
申请号：US863750
申请日：1986-05-16
申请人： Calvin P. H. Vary , Steven E. Diamond
发明人： Calvin P. H. Vary , Steven E. Diamond
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12Q1/6813 , C12Q1/6869 , Y10S435/81
摘要： A probe polynucleotide binds to a target nucleotide sequence in the nucleic acid of a biological sample, and then is enzymatically extended in the 3'-direction with a mixture of nucleoside triphosphates including at least one nucleoside triphosphate that has been detectably labeled. After separating extended hybrid from unreacted nucleoside triphosphates, detectably-modified nucleotides which have been incorporated are determined. In some forms, the 3'-terminal nucleotide of the probe polynucleotide is selected to form a matched pair with some sample strands, but a mismatched pair with other sample strands. In such cases, if the primer dependent enzyme used for extension is one lacking 3'-exonuclease activity, then only those hybrids forming such a matched pair will be extended and subsequently determined.
摘要翻译：探针多核苷酸与生物样品的核酸中的靶核苷酸序列结合，然后在包含至少一个可检测标记的核苷三磷酸的核苷三磷酸的混合物的3'-方向上酶促延伸。在从未反应的核苷三磷酸分离延长的杂交体后，测定已经掺入的可检测修饰的核苷酸。在某些形式中，选择探针多核苷酸的3'-末端核苷酸以与一些样品链形成匹配对，但与其它样品链成错配对。在这种情况下，如果用于延伸的引物依赖性酶是缺乏3'核酸外切酶活性的一种，则只有那些形成这种匹配对的杂交体将被扩展并随后确定。

8. 发明授权

US4795701A Homogeneous polynucleotide displacement assay method kit and reagent complex 失效
标题翻译：均质多核苷酸位移测定方法试剂盒和试剂复合物
公开(公告)号：US4795701A
公开(公告)日：1989-01-03
申请号：US755996
申请日：1985-07-17
申请人： Calvin P. H. Vary
发明人： Calvin P. H. Vary
IPC分类号： G01N33/50 , C12Q1/00 , C12Q1/48 , C12Q1/68 , G01N33/58 , C12Q1/34 , C12Q1/42
CPC分类号： C12Q1/6823 , C12Q1/48 , Y10S435/81
摘要： An RNA signal strand is displaced from a probe strand by a target nucleotide sequence. Without separation, a digestion enzyme selective for displaced RNA, e.g., a polynucleotide phosphorylase, digests the displaced RNA to nucleoside phosphates including ADP. The digestion products are detected, e.g. by phosphorylating the ADP to ATP and detecting the ATP by bioluminescence.
摘要翻译： RNA信号链通过靶核苷酸序列从探针链中移位。没有分离，对位移的RNA，例如多核苷酸磷酸化酶选择性的消化酶，将置换的RNA消化到包括ADP的核苷磷酸盐。检测消化产物，例如通过将ADP磷酸化成ATP并通过生物发光检测ATP。

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