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1. 发明授权

US5955329A Engineering plant thioesterases for altered substrate specificity 失效
标题翻译：工程植物硫酯酶改变底物特异性
公开(公告)号：US5955329A
公开(公告)日：1999-09-21
申请号：US440845
申请日：1995-05-15
申请人： Ling Yuan , Katayoon Dehesh , Jean Kridl , Vic Knauf
发明人： Ling Yuan , Katayoon Dehesh , Jean Kridl , Vic Knauf
IPC分类号： C12N9/16 , C12N15/82 , C12N15/00 , C07H21/04
CPC分类号： C12N15/8247 , C12N9/16
摘要： Methods of altering substrate specificity of plant acyl-ACP thioesterases, and engineered plant acyl-ACP thioesterases so produced are provided. The C-terminal two thirds portion of plant thioesterases is identified as desirable for such modifications. DNA sequences and constructs for expression of engineered thioesterases, as well as the novel thioesterases produced therefrom are also provided. Such DNA sequences may be used for expression of the engineered thioesterases in host cells, particularly seed cells of oilseed crop plants, for the modification of fatty acid composition. A C12 preferring plant acyl-ACP thioesterase described herein may be altered to obtain a plant thioesterase having approximately equal activity on C14 and C12 substrates. Further modification of the C12 enzyme yields a thioesterase having greater activity on C14 as compared to C12 substrates. Also provided in the instant invention are novel plant acyl-ACP thioesterase sequences from Cuphea palustris and mangosteen (Garcinia mangifera), which demonstrate desirable thioesterase activity profiles for plant genetic engineering applications.
摘要翻译：提供了改变植物酰基-ACP硫酯酶底物特异性的方法，以及如此制备的工程化植物酰基-ACP硫酯酶。植物硫酯酶的C末端三分之二部分被鉴定为对于这种修饰是理想的。还提供了用于表达工程化硫酯酶的DNA序列和构建体，以及由其产生的新的硫酯酶。这样的DNA序列可用于在宿主细胞，特别是油籽作物植物的种子细胞中表达改性的硫酯酶，用于改变脂肪酸组成。可以改变本文所述的C12优选植物酰基-ACP硫酯酶以获得对C14和C12底物具有大致相同活性的植物硫酯酶。与C12底物相比，C12酶的进一步修饰产生对C14具有更大活性的硫酯酶。本发明还提供了来自Cuphea palustris和山竹（Garcinia mangifera）的新型植物酰基-ACP硫酯酶序列，其显示用于植物基因工程应用的期望的硫酯酶活性谱。

2. 发明授权

US5723761A Plant acyl-ACP thioesterase sequences 失效
标题翻译：植物酰基-ACP硫酯酶序列
公开(公告)号：US5723761A
公开(公告)日：1998-03-03
申请号：US464523
申请日：1995-06-02
申请人： Toni A. Voelker , Ling Yuan , Jean Kridl , Deborah Hawkins , Aubrey Jones
发明人： Toni A. Voelker , Ling Yuan , Jean Kridl , Deborah Hawkins , Aubrey Jones
IPC分类号： A01H5/00 , A23D9/00 , C11C3/00 , C12N5/10 , C12N9/16 , C12N15/09 , C12N15/55 , C12N15/82 , A01H5/10 , C12N5/14 , C12N15/29 , C12N15/52
CPC分类号： A23D9/00 , C12N15/8247 , C12N9/16
摘要： This invention relates to plant thioesterases, specifically plant acyl-ACP thioesterases having substantial activity on palmitoyl-ACP substrates. DNA constructs useful for the expression of a plant palmitoyl-ACP thioesterase in a plant seed cell are described. Such constructs will contain a DNA sequence encoding the plant palmitoyl-ACP thioesterase of interest under the control of regulatory elements capable of preferentially directing the expression of the plant palmitoyl-ACP thioesterase in seed tissue, as compared with other plant tissues, when such a construct is expressed in a transgenic plant. This invention also relates to methods of using a DNA sequence encoding a plant palmitoyl-ACP thioesterase for the modification of the proportion of free fatty acids produced in a plant seed cell. Plant palmitoyl-ACP thioesterase sequences exemplified herein include Cuphea, leek, mango and elm. Transgenic plants having increased levels of C16:0 fatty acids in their seeds as the result of expression of these palmitoyl-ACP thioesterase sequences are also provided.
摘要翻译：本发明涉及植物硫酯酶，特别是具有对棕榈酰-ACP底物具有显着活性的植物酰基-ACP硫酯酶。描述了可用于在植物种子细胞中表达植物棕榈酰-ACP硫酯酶的DNA构建体。与其他植物组织相比，这样的构建体将含有编码植物棕榈酰-ACP硫酯酶的DNA序列，该调控元件能够优先指导植物棕榈酰-ACP硫酯酶在种子组织中的表达，在转基因植物中表达。本发明还涉及使用编码植物棕榈酰-ACP硫酯酶的DNA序列来修饰植物种子细胞中产生的游离脂肪酸的比例的方法。本文例示的植物棕榈酰-ACP硫酯酶序列包括Cuphea，韭葱，芒果和榆树。还提供了作为这些棕榈酰-ACP硫酯酶序列表达的结果，在其种子中具有增加的C16：0脂肪酸水平的转基因植物。

3. 发明授权

US5538878A Superoxide dismutase expression in plants 失效
标题翻译：植物中超氧化物歧化酶表达
公开(公告)号：US5538878A
公开(公告)日：1996-07-23
申请号：US381827
申请日：1989-07-19
申请人： Bruce Thomas , H. Maelor Davies , Jean Kridl , Jeffery K. O'Neal , C. Jacques Van Assche
发明人： Bruce Thomas , H. Maelor Davies , Jean Kridl , Jeffery K. O'Neal , C. Jacques Van Assche
IPC分类号： A01H1/00 , A01H1/04 , A01H5/00 , C12N1/00 , C12N5/00 , C12N5/10 , C12N9/02 , C12N15/00 , C12N15/09 , C12N15/53 , C12N15/82 , C12P19/00 , C12N15/31
CPC分类号： C12N9/0089 , C12N15/8271 , C12N15/8273
摘要： Plant species having enhanced superoxide dismutase activity as a result of transformation with a DNA expression cassette comprising an E. coli MnSOD gene are provided. Transportation of the expression product of the gene may be targeted to a specific cell organelle, such as the chloroplast.
摘要翻译：提供了具有由包含大肠杆菌MnSOD基因的DNA表达盒转化的结果而具有增强的超氧化物歧化酶活性的植物物种。基因表达产物的转运可以靶向特定的细胞器如叶绿体。

4. 发明申请

US20090214744A1 Methods for increasing stearate content in soybean oil 审中-公开
公开(公告)号：US20090214744A1
公开(公告)日：2009-08-27
申请号：US12320692
申请日：2009-02-02
申请人： Jean Kridl
发明人： Jean Kridl
IPC分类号： A23D9/00 , C11B1/00 , A01G1/00 , A01D45/22
CPC分类号： C12N15/8247 , C12N9/16
摘要： This invention relates to a method for increasing stearate as a component of total triglycerides found in soybean seed. The method generally comprises growing a soybean plant having integrated into its genome a DNA construct comprising, in the 5′ to 3′ direction of transcription, a promoter functional in a soybean plant seed cell, a DNA sequence encoding an acyl-ACP thioesterase protein having substantial activity on C18:0 acyl-ACP substrates, and a transcription termination region functional in a plant cell. The present invention also provides a soybean seed with about 33 weight percent or greater stearate as a component of total fatty acids found in seed triglycerides.

5. 发明授权

US5981236A Geminivirus-based gene expression system 失效
标题翻译：基于双子病毒的基因表达系统
公开(公告)号：US5981236A
公开(公告)日：1999-11-09
申请号：US896753
申请日：1997-07-18
申请人： Jean Kridl , Vic C. Knauf , George Breuning
发明人： Jean Kridl , Vic C. Knauf , George Breuning
IPC分类号： C12N9/16 , C12N15/82 , C12N15/83 , C12N5/10 , C12N15/64
CPC分类号： C12N9/16 , C12N15/8203 , C12N15/8216 , C12N15/8247
摘要： A geminivirus based vector system for obtaining controlled expression of a nucleic acid fragment of interest is disclosed. Tissue specific regulatory regions are identified employing cDNA screening and the resulting tissue-specific regulatory regions are manipulated for use in geminivirus constructs to provide for transcription and/or expression of nucleic acid sequences nonindigenous to the geminivirus vector for introduction into plant cells. The vector system may be used to provide transformed plants having cells, tissues or parts with a modified phenotypic property.
摘要翻译：公开了一种用于获得感兴趣的核酸片段的受控表达的基于双恶案病毒的载体系统。使用cDNA筛选鉴定组织特异性调节区域，并且操作所得到的组织特异性调节区域用于双因素病毒构建体，以提供非双偶因病毒载体的本身导入植物细胞的核酸序列的转录和/或表达。载体系统可用于提供具有改变的表型性质的具有细胞，组织或部分的转化植物。

6. 发明申请

US20070162995A1 Nitrogen-efficient monocot plants 有权
标题翻译：氮肥单子叶植物
公开(公告)号：US20070162995A1
公开(公告)日：2007-07-12
申请号：US11644321
申请日：2006-12-21
申请人： Allen Good , Mary Depauw , Jean Kridl , George Theodoris , Ashok Shrawat
发明人： Allen Good , Mary Depauw , Jean Kridl , George Theodoris , Ashok Shrawat
IPC分类号： A01H5/00 , C12N9/10 , C12N15/82 , C12N5/04
CPC分类号： C12N15/8262 , C12N9/1096 , C12N15/8227 , C12N15/8243 , C12N15/8261 , Y02A40/146
摘要： Methods of increasing nitrogen utilization efficiency in monocot plants through genetic modification to increase the levels of alanine aminotransferase expression and plants produced there from are described. In particular, methods for increasing the biomass and yield of transgenic monocot plants grown under nitrogen limiting conditions compared to non-transgenic plants are described. In this way, monocot plants may be produced that maintain a desired yield while reducing the need for high levels of nitrogen application.
摘要翻译：描述了通过遗传修饰增加单子叶植物中氮利用效率以增加丙氨酸氨基转移酶表达水平的方法及其生产的植物。特别地描述了与非转基因植物相比，用于增加在氮限制条件下生长的转基因单子叶植物的生物量和产量的方法。以这种方式，可以生产保持所需产率的单子叶植物，同时减少对高氮施用量的需要。

7. 发明申请

US20050177902A1 Methods of producing specialty grain to segment markets 审中-公开
标题翻译：生产专业粮食来分割市场的方法
公开(公告)号：US20050177902A1
公开(公告)日：2005-08-11
申请号：US10991889
申请日：2004-11-18
申请人： Troy Lohrmann , Jean Kridl , Thomas Savage , Daniel Dyer
发明人： Troy Lohrmann , Jean Kridl , Thomas Savage , Daniel Dyer
IPC分类号： A01H1/00 , A01H5/00 , A01H5/10 , A23K1/14 , A23K1/16 , A23K1/18 , A23L20060101 , C12N15/82 , G06F17/60
CPC分类号： A01H5/10 , A23K10/30 , A23K20/158 , A23K20/179 , A23K50/30 , A23K50/75
摘要： The present invention provides a method of segmenting a market for a grain by manipulating one or more phenotypic characteristics of the grain. The phenotypic characteristics include oil content, pigmentation potential, iodine value, and linoleic acid content.
摘要翻译：本发明提供了一种通过操纵谷物的一种或多种表型特征来分割谷物市场的方法。表型特征包括油含量，色素沉着势，碘值和亚油酸含量。

8. 发明授权

US07504563B1 Methods for increasing stearate content in soybean oil 有权
公开(公告)号：US07504563B1
公开(公告)日：2009-03-17
申请号：US11305021
申请日：2005-12-19
申请人： Jean Kridl
发明人： Jean Kridl
IPC分类号： A01H5/00 , C12N15/82
CPC分类号： C12N15/8247 , C12N9/16
摘要： This invention relates to a method for increasing stearate as a component of total triglycerides found in soybean seed. The method generally comprises growing a soybean plant having integrated into its genome a DNA construct comprising, in the 5′ to 3′ direction of transcription, a promoter functional in a soybean plant seed cell, a DNA sequence encoding an acyl-ACP thioesterase protein having substantial activity on C18:0 acyl-ACP substrates, and a transcription termination region functional in a plant cell. The present invention also provides a soybean seed with about 33 weight percent or greater stearate as a component of total fatty acids found in seed triglycerides.

9. 发明授权

US06380462B1 Method for increasing stearate content in soybean oil 有权
标题翻译：增加大豆油中硬脂酸含量的方法
公开(公告)号：US06380462B1
公开(公告)日：2002-04-30
申请号：US09359070
申请日：1999-07-22
申请人： Jean Kridl
发明人： Jean Kridl
IPC分类号： C12N1582
CPC分类号： C12N15/8247 , C12N9/16
摘要： This invention relates to a method for increasing stearate as a component of total triglycerides found in soybean seed. The method generally comprises growing a soybean plant having integrated into its genome a DNA construct comprising, in the 5′ to 3′ direction of transcription, a promoter functional in a soybean plant seed cell, a DNA sequence encoding an acyl-ACP thioesterase protein having substantial activity on C18:0 acyl-ACP substrates, and a transcription termination region functional in a plant cell. The present invention also provides a soybean seed with about 33 weight percent or greater stearate as a component of total fatty acids found in seed triglycerides.
摘要翻译：本发明涉及一种增加作为大豆种子中总甘油三酯的组分的硬脂酸酯的方法。该方法通常包括生长大豆植物，其整合到其基因组中，DNA构建体在5'至3'转录方向上包含在大豆植物种子细胞中起作用的启动子，编码酰基-ACP硫酯酶蛋白质的DNA序列，其具有对C18：0酰基-ACP底物的活性以及在植物细胞中功能的转录终止区。本发明还提供一种大豆种子，其具有约33重量％或更大的硬脂酸酯作为在种子甘油三酯中发现的总脂肪酸的组分。

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