会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 1. 发明申请
    • Methods and apparatus for reference lab diagnostics
    • 参考实验室诊断的方法和装置
    • US20060292649A1
    • 2006-12-28
    • US11431343
    • 2006-05-09
    • Linda CahillRoger O'Neill
    • Linda CahillRoger O'Neill
    • G01N33/558
    • G01N33/54366B01L3/502715B01L3/50273B01L2200/0668B01L2400/0406B01L2400/0418B01L2400/0421B01L2400/0487G01N27/44726G01N27/44795G01N33/558G01N33/582G01N33/6896G01N2800/2828Y02A50/57
    • Automated protein assay apparatus and methods suitable for use in a reference lab are described. A screening test for a pathogen or disease condition is performed at the reference lab. If the results of the screening test are positive, a definitive assay is performed. One or more analytes are resolved in a fluid path such as that of a capillary by isoelectric focusing. The resolved analytes are immobilized in the capillary by photoimmobilization. A typical analyte is a protein of a biological sample of a subject. Detection agents such as antibodies are flowed through the capillary which bind to or interact with the analytes, forming antibody-protein complexes. A chemiluminescent substrate is flowed through the capillary and detected with a photon detector. The detected locations of the analytes are recorded and the results sent to the referring physician. The assay is preferably performed by a computer-controlled, automated system exhibiting speed, precision and repeatability which are highly desirable for a reference lab diagnostic test.
    • 描述适用于参考实验室的自动蛋白质测定装置和方法。 在参考实验室进行病原体或疾病状况的筛选试验。 如果筛选试验的结果为阳性,则进行确定的测定。 一个或多个分析物通过等电聚焦在诸如毛细管的流体路径中解析。 通过光固化将解析的分析物固定在毛细管中。 典型的分析物是受试者的生物样品的蛋白质。 诸如抗体的检测剂流过毛细管,其结合或与分析物相互作用,形成抗体 - 蛋白质复合物。 化学发光底物流过毛细管并用光子检测器检测。 记录检测到的分析物的位置,并将结果发送给转诊医师。 该测定优选通过显示速度,精度和重复性的计算机控制的自动化系统进行,这对于参考实验室诊断测试是非常需要的。
    • 5. 发明授权
    • Electrophoretic nucleic acid purification method
    • 电泳核酸纯化方法
    • US6146511A
    • 2000-11-14
    • US016531
    • 1998-01-30
    • Gary SlaterJ. William EfcavitchGuy DrouinPascal MayerJean RousseauHong Yan ZhouClaudia ChiesaRobert RuhfelRoger O'Neill
    • Gary SlaterJ. William EfcavitchGuy DrouinPascal MayerJean RousseauHong Yan ZhouClaudia ChiesaRobert RuhfelRoger O'Neill
    • B01D57/02B03C5/00C07H21/02C07H21/04C12N15/09C12N15/10G01N27/447G01N27/26
    • C12N15/101G01N27/447
    • An electrophoretic method for purifying a nucleic acid sample is disclosed. The method generally comprises the steps of (1) providing a nucleic acid sample comprising a desired nucleic acid and one or more contaminants, (2) providing an electrophoresis matrix having a loading well and a recovery well formed therein, (3) placing the nucleic acid sample into the loading well, (4) performing a first electrophoresis comprising electrophoresing the nucleic acid sample for a first time effective to transport the desired nucleic acid out of the loading well and into the electrophoresis matrix; and (5) performing a second electrophoresis comprising electrophoresing the nucleic acid sample for a second time effective to transport the desired nucleic acid out of the electrophoresis matrix and into the recovery well. According to the method, the first and second electrophoresis steps are effective to substantially reduce the concentration of contaminants relative to the concentration of desired nucleic acid in the nucleic acid sample, thereby producing a purified nucleic acid. In the method, the loading and recovery wells may be the same or different, and the electric fields may be DC or alternating. Also disclosed is a preparative electrophoresis method employing an alternating electrical field.
    • 公开了一种用于纯化核酸样品的电泳方法。 该方法通常包括以下步骤:(1)提供包含所需核酸和一种或多种污染物的核酸样品,(2)提供具有载体孔的电泳基质和其中形成的回收孔,(3) (4)进行第一次电泳,其中包括使核酸样品首次有效地将所需核酸转运到载体孔中并进入电泳基质中; 包括进行第二次电泳,其包括将核酸样品电泳第二次,有效将所需核酸转运到电泳基质内并进入回收井。 根据该方法,第一和第二电泳步骤对于相对于核酸样品中所需核酸浓度显着降低污染物的浓度是有效的,从而产生纯化的核酸。 在该方法中,加载和回收井可以相同或不同,并且电场可以是直流或交替的。 还公开了采用交流电场的制备电泳方法。