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1. 发明授权

US07776547B2 Cellular analysis using Raman surface scanning 有权
标题翻译：使用拉曼表面扫描的细胞分析
公开(公告)号：US07776547B2
公开(公告)日：2010-08-17
申请号：US11027470
申请日：2004-12-30
申请人： Mark Roth , Andrew Berlin , Selena Chan , Tae-Woong Koo , Xing Su , Lei Sun
发明人： Mark Roth , Andrew Berlin , Selena Chan , Tae-Woong Koo , Xing Su , Lei Sun
IPC分类号： G01N33/53
CPC分类号： G01N33/54366 , G01N33/54373 , G01N33/58 , Y10T436/10
摘要： Methods and apparatus are provided for assaying cell samples, which may be living cells, using probes labeled with composite organic-inorganic nanoparticles (COINs) and microspheres with COINs embedded within a polymer matrix to which the probe moiety is attached. COINs intrinsically produce SERS signals upon laser irradiation, making COIN-labeled probes particularly suitable in a variety of methods for assaying cells, including biological molecules that may be contained on or within cells, most of which are not inherently Raman-active. The invention provides variations of the sandwich immunoassay employing both specific and degenerate binding, methods for reverse phase assay of tissue samples and cell microstructures, in solution displacement and competition assays, and the like. Systems and chips useful for practicing the invention assays are also provided.
摘要翻译：提供了用于使用用复合有机 - 无机纳米颗粒（COIN）标记的探针和嵌入探针部分所连接的聚合物基质内的COIN的微球来测定可能为活细胞的细胞样品的方法和装置。 COIN在激光照射下固有地产生SERS信号，使得COIN标记的探针特别适用于多种测定细胞的方法，包括可能包含在细胞内或细胞内的生物分子，其中大部分不是固有的拉曼活性的。本发明提供使用特异性和简并结合的夹心免疫测定的变体，组织样品和细胞微结构的反相测定方法，溶液置换和竞争测定等。还提供了用于实施本发明测定的系统和芯片。

2. 发明授权

US07432113B2 Surface modification of metals for biomolecule detection using surface enhanced Raman scattering (SERS) 失效
公开(公告)号：US07432113B2
公开(公告)日：2008-10-07
申请号：US11134749
申请日：2005-05-19
申请人： Tae-Woong Koo , Selena Chan , Xing Su , Jingwu Zhang , Lei Sun
发明人： Tae-Woong Koo , Selena Chan , Xing Su , Jingwu Zhang , Lei Sun
IPC分类号： G01N33/553
CPC分类号： G01N21/658
摘要： The present invention is based on the discovery that the methods described herein for the production of metallic colloids result in colloids exhibiting increased signal enhancement and reproducibility for the SERS detection of biomolecules. Thus, using the methods of the invention, a wide variety of biomolecules can be detected with a greater sensitivity and reliability.

3. 发明申请

US20080241569A1 ENCAPSULATION OF RAMAN ACTIVE NANOPARTICLES 审中-公开
标题翻译：拉曼活性纳米颗粒的包封
公开(公告)号：US20080241569A1
公开(公告)日：2008-10-02
申请号：US11694293
申请日：2007-03-30
申请人： Haoyu Qin , Jingwu Zhang , Claire E. Dentinger , Lei Sun , Xing Su
发明人： Haoyu Qin , Jingwu Zhang , Claire E. Dentinger , Lei Sun , Xing Su
IPC分类号： B32B5/16
CPC分类号： G01N21/658 , B82Y15/00 , B82Y30/00 , Y10T428/12028 , Y10T428/2991
摘要： Embodiments of the present invention provide methods for coating nanoparticles with polymeric coatings and nanoparticles that are coated with polymeric coatings. The polymeric coatings typically comprise two or more layers wherein the first layer has a charge that is opposite to that of the second layer. In further embodiments, the nanoparticles that can act as labels or reporters are coated with polymeric coatings. Optionally, these reporter or label nanoparticles may be Raman-active, such that they provide a distinctive Raman signature upon excitation with electromagnetic radiation.
摘要翻译：本发明的实施方案提供了用聚合物涂层和纳米颗粒涂覆聚合物涂层的方法。聚合物涂层通常包含两层或更多层，其中第一层具有与第二层相反的电荷。在另外的实施方案中，可以用作标记或报告物的纳米颗粒涂覆有聚合物涂层。任选地，这些报告物或标记纳米颗粒可以是拉曼活性的，使得它们在用电磁辐射激发时提供独特的拉曼特征。

4. 发明申请

US20070054288A1 Programmable molecular barcodes 审中-公开
标题翻译：可编程分子条形码
公开(公告)号：US20070054288A1
公开(公告)日：2007-03-08
申请号：US11430612
申请日：2006-05-08
申请人： Xing Su , Tae-Woong Koo , Andrew Berlin , Lei Sun , Narayanan Sundararajan , Mineo Yamakawa
发明人： Xing Su , Tae-Woong Koo , Andrew Berlin , Lei Sun , Narayanan Sundararajan , Mineo Yamakawa
IPC分类号： C12Q1/68 , C12M1/34 , G06K7/10
CPC分类号： B82Y10/00 , B82Y5/00 , C12Q1/6816 , G06K19/06028 , C12Q2525/161 , C12Q2537/143 , C12Q2565/1025
摘要： The present disclosure concerns methods for producing and/or using molecular barcodes. In certain embodiments of the invention, the barcodes comprise polymer backbones that may contain one or more branch structures. Tags may be attached to the backbone and/or branch structures. The barcode may also comprise a probe that can bind to a target, such as proteins, nucleic acids and other biomolecules or aggregates. Different barcodes may be distinguished by the type and location of the tags. In other embodiments, barcodes may be produced by hybridization of one or more tagged oligonucleotides to a template, comprising a container section and a probe section. The tagged oligonucleotides may be designed as modular code sections, to form different barcodes specific for different targets. In alternative embodiments, barcodes may be prepared by polymerization of monomeric units. Bound barcodes may be detected by various imaging modalities, such as, surface plasmon resonance, fluorescent or Raman spectroscopy.
摘要翻译：本公开涉及用于生产和/或使用分子条形码的方法。在本发明的某些实施方案中，条形码包含可包含一个或多个分支结构的聚合物主链。标签可以附加到骨干和/或分支结构。条形码还可以包含可以与靶标结合的探针，例如蛋白质，核酸和其他生物分子或聚集体。可以通过标签的类型和位置区分不同的条形码。在其它实施方案中，条形码可以通过将一个或多个标记的寡核苷酸与包含容器部分和探针部分的模板杂交来产生。标记的寡核苷酸可以被设计为模块代码部分，以形成针对不同靶标的不同条形码。在替代实施例中，条形码可以通过单体单元的聚合来制备。可以通过各种成像方式，例如表面等离子体共振，荧光或拉曼光谱来检测结合条形码。

5. 发明申请

US20070048746A1 Multiplex data collection and analysis in bioanalyte detection 有权
标题翻译：生物分析仪检测中的多重数据收集和分析
公开(公告)号：US20070048746A1
公开(公告)日：2007-03-01
申请号：US11216112
申请日：2005-09-01
申请人： Xing Su , Lei Sun , Mineo Yamakawa , Jingwu Zhang , Qing Ma , Tae-Woong Koo , Richard Jones
发明人： Xing Su , Lei Sun , Mineo Yamakawa , Jingwu Zhang , Qing Ma , Tae-Woong Koo , Richard Jones
IPC分类号： C12Q1/68 , G01N33/53 , C12M1/34
CPC分类号： G01N33/53 , B82Y15/00 , B82Y30/00 , G01N33/54366 , G01N33/5438
摘要： Method and device to collect multiplex data simultaneously in analyte detection and analyze the data by experimentally trained software (machine-learning) is disclosed. Various ways (magnetic particles and microcoils) are disclosed to collect multiple reporter (tag) signals. Multiplex detection can increase the biomolecule analysis efficiency by using small sample size and saving assay reagents and time. Machine learning and data analysis schemes are also disclosed. Multiple affinity binding partners, each labeled by a unique reporter, are contacted with a sample and a green spectrum is taken to detect multiple reporter signals. The spectrum is deconvoluted by experimentally trained software to identify multiple analytes.
摘要翻译：公开了通过实验训练的软件（机器学习）在分析物检测中同时收集多重数据并分析数据的方法和装置。公开了各种方式（磁性颗粒和微型线圈）以收集多个报道（标签）信号。多重检测可以通过使用小样本量和节省测定试剂和时间来增加生物分子分析效率。还披露了机器学习和数据分析方案。将多个亲和力结合配偶体（各自由唯一报道分子标记）与样品接触，并采用绿色光谱来检测多个报道信号。通过实验训练的软件对光谱进行去卷积，以识别多个分析物。

6. 发明申请

US20060009914A1 Detection and identification of nucleic acid, peptide, and protein modifications 审中-公开
标题翻译：检测和鉴定核酸，肽和蛋白质修饰
公开(公告)号：US20060009914A1
公开(公告)日：2006-01-12
申请号：US10919699
申请日：2004-08-16
申请人： Narayan Sundararajan , Lei Sun , Xing Su , Mineo Yamakawa , Zhang Jingwu , Selena Chan , Andrew Berlin , Tae-Woong Koo , Mark Roth , Phil Gafken
发明人： Narayan Sundararajan , Lei Sun , Xing Su , Mineo Yamakawa , Zhang Jingwu , Selena Chan , Andrew Berlin , Tae-Woong Koo , Mark Roth , Phil Gafken
IPC分类号： G06F19/00 , G01N33/48
CPC分类号： G01N33/6842 , G01N33/6803 , G01N33/6818 , Y02A90/26
摘要： Embodiments of the present invention provide devices and methods for detecting, identifying, distinguishing, and quantifying modifications to nucleic acids, proteins, and peptides using SERS and Raman spectroscopy. Applications of embodiments of the present invention include proteome wide modification profiling and analyses with applications in disease diagnosis, prognosis and drug efficacy studies, enzymatic activity profiling and assays.
摘要翻译：本发明的实施方案提供用于使用SERS和拉曼光谱检测，鉴定，区分和定量对核酸，蛋白质和肽的修饰的装置和方法。本发明实施方案的应用包括在疾病诊断，预后和药物功效研究，酶活性分析和测定中的应用的蛋白质组广泛修饰分析和分析。

7. 发明申请

US20050221506A1 Surface modification of metals for biomolecule detection using surface enhanced Raman scattering (SERS) 有权
公开(公告)号：US20050221506A1
公开(公告)日：2005-10-06
申请号：US10814695
申请日：2004-03-30
申请人： Tae-Woong Koo , Selena Chan , Xing Su , Jingwu Zhang , Lei Sun
发明人： Tae-Woong Koo , Selena Chan , Xing Su , Jingwu Zhang , Lei Sun
IPC分类号： G01N21/65 , G01N33/553
CPC分类号： G01N21/658
摘要： The present invention is based on the discovery that the methods described herein for the production of metallic colloids result in colloids exhibiting increased signal enhancement and reproducibility for the SERS detection of biomolecules. Thus, using the methods of the invention, a wide variety of biomolecules can be detected with a greater sensitivity and reliability.

8. 发明申请

US20050151126A1 Methods of producing carbon nanotubes using peptide or nucleic acid micropatterning 审中-公开
标题翻译：使用肽或核酸微图案生产碳纳米管的方法
公开(公告)号：US20050151126A1
公开(公告)日：2005-07-14
申请号：US10750141
申请日：2003-12-31
申请人： Mineo Yamakawa , Yeugang Zhang , Xing Su , Lei Sun , Andrew Berlin , Narayanan Sundararajan
发明人： Mineo Yamakawa , Yeugang Zhang , Xing Su , Lei Sun , Andrew Berlin , Narayanan Sundararajan
IPC分类号： C01B31/02 , D01F9/127 , H01L51/00 , H01L51/30 , D01F9/12 , H01L29/06
CPC分类号： B82Y40/00 , B82Y10/00 , B82Y30/00 , C01B32/162 , C01B2202/08 , C01B2202/36 , D01F9/127 , H01L51/0048 , H01L51/0052
摘要： The methods, apparatus and systems disclosed herein concern ordered arrays of carbon nanotubes. In particular embodiments of the invention, the nanotube arrays are formed by a method comprising attaching catalyst nanoparticles 140, 230 to polymer 120, 210 molecules, attaching the polymer 120, 210 molecules to a substrate, removing the polymer 120, 210 molecules and producing carbon nanotubes on the catalyst nanoparticles 140, 230. The polymer 120, 210 molecules can be attached to the substrate in ordered patterns, using self-assembly or molecular alignment techniques. The nanotube arrays can be attached to selected areas 110, 310 of the substrate. Within the selected areas 110, 310, the nanotubes are distributed non-randomly. Other embodiments disclosed herein concern apparatus that include ordered arrays of nanotubes attached to a substrate and systems that include ordered arrays of carbon nanotubes attached to a substrate, produced by the claimed methods. In certain embodiments, provided herein are methods for aligning a molecular wire, by ligating the molecular wire to a double stranded DNA molecule.
摘要翻译：本文公开的方法，装置和系统涉及碳纳米管的有序阵列。在本发明的具体实施方案中，纳米管阵列通过包括将催化剂纳米颗粒140,230连接到聚合物120,210分子，将聚合物120,210分子连接到基底上的方法形成，除去聚合物120,210分子并产生碳催化剂纳米颗粒140,230上的纳米管。聚合物120,210分子可以使用自组装或分子对准技术以有序图案附着到基底上。纳米管阵列可以附着到基板的选定区域110,310。在所选择的区域110,310内，纳米管是非随机分布的。本文公开的其它实施方案涉及包括连接到衬底的纳米管的有序阵列和包括通过所要求保护的方法产生的连接到衬底的碳纳米管的有序阵列的系统的装置。在某些实施方案中，本文提供了通过将分子线连接到双链DNA分子来对齐分子线的方法。

9. 发明授权

US08062841B2 Multiplex data collection and analysis in bioanalyte detection 有权
标题翻译：生物分析仪检测中的多重数据收集和分析
公开(公告)号：US08062841B2
公开(公告)日：2011-11-22
申请号：US12147440
申请日：2008-06-26
申请人： Xing Su , Lei Sun , Mineo Yamakawa , Jingwu Zhang , Qing Ma , Tae-Woong Koo , Richard Jones
发明人： Xing Su , Lei Sun , Mineo Yamakawa , Jingwu Zhang , Qing Ma , Tae-Woong Koo , Richard Jones
IPC分类号： C12Q1/68 , G01N33/53 , C07H21/02 , C07H21/04 , C07K14/00
CPC分类号： G01N33/53 , B82Y15/00 , B82Y30/00 , G01N33/54366 , G01N33/5438
摘要： Method and device to collect multiplex data simultaneously in analyte detection and analyze the data by experimentally trained software (machine-learning) is disclosed. Various ways (magnetic particles and microcoils) are disclosed to collect multiple reporter (tag) signals. Multiplex detection can increase the biomolecule analysis efficiency by using small sample size and saving assay reagents and time. Machine learning and data analysis schemes are also disclosed. Multiple affinity binding partners, each labeled by a unique reporter, are contacted with a sample and a single spectrum is taken to detect multiple reporter signals. The spectrum is deconvoluted by experimentally trained software to identify multiple analytes.
摘要翻译：公开了通过实验训练的软件（机器学习）在分析物检测中同时收集多重数据并分析数据的方法和装置。公开了各种方式（磁性颗粒和微型线圈）以收集多个报道（标签）信号。多重检测可以通过使用小样本量和节省测定试剂和时间来增加生物分子分析效率。还披露了机器学习和数据分析方案。将多个亲和力结合配偶体（每个由唯一报道分子标记）与样品接触，并采用单个谱来检测多个报道信号。通过实验训练的软件对光谱进行去卷积，以识别多个分析物。

10. 发明申请

US20090303461A1 DETECTION OF ENHANCED MULTIPLEX SIGNALS BY SURFACE ENHANCED RAMAN SPECTROSCOPY (SERS) 审中-公开
标题翻译：通过表面增强拉曼光谱法检测增强多重信号（SERS）
公开(公告)号：US20090303461A1
公开(公告)日：2009-12-10
申请号：US12359503
申请日：2009-01-26
申请人： Lei Sun , Xing Su
发明人： Lei Sun , Xing Su
IPC分类号： G01N1/44 , G01N21/65 , C07H21/02 , C07H21/04 , C07K14/00 , C07K16/00 , C07H1/00
CPC分类号： G01N21/658 , G01N33/54306
摘要： Various methods of using Raman-active or SERS-active probe constructs to detect analytes in biological samples, such as the nucleic acid and/or protein-containing analytes in a body fluid are provided.
摘要翻译：提供了使用拉曼活性或SERS-活性探针构建体检测生物样品中的分析物的各种方法，例如体液中的核酸和/或蛋白质分析物。

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