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    • 6. 发明授权
    • Electrophoresis methods
    • 电泳法
    • US07410559B2
    • 2008-08-12
    • US10479245
    • 2001-05-29
    • Mari TabuchiYoshinobu Baba
    • Mari TabuchiYoshinobu Baba
    • G01N27/447
    • G01N27/44747
    • A separation carrier comprising β-1,3-glucan and/or methyl cellulose; a running buffer comprising the separation carrier; a method of capillary electrophoresis or microchip electrophoresis, wherein a sample comprising macromolecular compounds is run in the presence of the running buffer; a method of capillary electrophoresis or microchip electrophoresis, comprising the step of injecting the sample by an electrical injection or application of pressure; and a method for analyzing macromolecular compounds by the method of capillary electrophoresis or microchip electrophoresis. According to the present invention, it is possible to achieve high resolution quickly. Therefore, the methods are useful in the High Through-put screening analysis of proteins or sugar chains in gene analysis, proteome analysis or glycome analysis, and applicable in medical diagnostic apparatuses and the elucidation of biological functions, mechanisms of onset of diseases, etc.
    • 包含β-1,3-葡聚糖和/或甲基纤维素的分离载体; 包括分离载体的运行缓冲器; 毛细管电泳或微芯片电泳的方法,其中包含高分子化合物的样品在运行缓冲液存在下运行; 一种毛细管电泳或微芯片电泳的方法,包括通过电注射或施加压力注射样品的步骤; 以及通过毛细管电泳或微芯片电泳法分析高分子化合物的方法。 根据本发明,可以快速地实现高分辨率。 因此,该方法在蛋白质或糖链的高通量筛选分析中可用于基因分析,蛋白质组分析或糖原分析,适用于医学诊断仪器,阐明生物学功能,疾病发病机制等。