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1. 发明授权

US10031139B2 Method for detecting biological material 有权
公开(公告)号：US10031139B2
公开(公告)日：2018-07-24
申请号：US14387691
申请日：2013-03-28
申请人： Konica Minolta, Inc. , Tohoku University
发明人： Kensaku Takanashi , Hideki Gouda , Keizou Takano , Hideki Hoshino , Yasushi Nakano , Kohsuke Gonda , Noriaki Ohuchi , Mika Watanabe
IPC分类号： G01N33/58 , G01N33/533 , G01N33/543 , B82Y30/00 , G01N1/30
摘要： The present invention provides a staining method in which the fluorescent staining properties in a fluorescently-immunostained specimen are not reduced even when an oil-based mounting medium is used. The present invention also provides a method of preventing deterioration of a fluorescent label caused by irradiation with excitation light and improving the light resistance in a fluorescently-immunostained specimen obtained by the staining method. The biological substance detection method according to the present invention is a biological substance detection method for specifically detecting a biological substance from a pathological specimen, which includes the steps of: immunostaining the specimen with a fluorescent label; immobilizing the thus stained specimen; and mounting the thus immobilized specimen using a mounting medium including an organic solvent not freely miscible with water. In the biological substance detection method, the above-described mounting medium further includes a discoloration inhibitor.

2. 发明授权

US09779500B2 Image processing device, pathological diagnosis support system, image processing program, and pathological diagnosis support method 有权
公开(公告)号：US09779500B2
公开(公告)日：2017-10-03
申请号：US14902410
申请日：2014-06-27
申请人： Konica Minolta, Inc.
发明人： Yasuhiro Watanabe , Takeshi Isoda , Kohsuke Gonda , Noriaki Ohuchi , Mika Watanabe
IPC分类号： G06K9/00 , A61B5/00 , G06T7/00 , G06T3/00 , G06K9/46 , G06K9/52 , G06K9/62 , G06T7/60 , G06T7/33 , G01N21/64
CPC分类号： G06T7/0012 , A61B5/0062 , A61B5/0071 , A61B5/7264 , A61B2576/00 , G01N21/6456 , G06K9/00134 , G06K9/0014 , G06K9/00147 , G06K9/4661 , G06K9/52 , G06K9/6215 , G06T3/0068 , G06T7/33 , G06T7/60 , G06T2207/10016 , G06T2207/10024 , G06T2207/10061 , G06T2207/10064 , G06T2207/20036 , G06T2207/20221 , G06T2207/30004 , G06T2207/30024
摘要： An image processing device includes an input unit and an alignment unit. The input unit inputs a cell shape image and a fluorescence image. The cell shape image shows a shape of a cell in a tissue section. The fluorescence image shows expression of a specific protein as a fluorescent bright point in a region same as a region in the tissue section. The alignment unit aligns the cell shape image and the fluorescence image based on an information source detected in both the cell shape image and the fluorescence image.

3. 发明申请

US20150079611A1 METHOD FOR DETECTING BIOLOGICAL MATERIAL 审中-公开
标题翻译：检测生物材料的方法
公开(公告)号：US20150079611A1
公开(公告)日：2015-03-19
申请号：US14387691
申请日：2013-03-28
申请人： Konica Minolta, Inc. , Tohoku University
发明人： Kensaku Takanashi , Hideki Gouda , Keizou Takano , Hideki Hoshino , Yasushi Nakano , Kohsuke Gonda , Noriaki Ohuchi , Mika Watanabe
IPC分类号： G01N33/543 , G01N33/58
CPC分类号： G01N33/54393 , B82Y30/00 , G01N1/30 , G01N33/533 , G01N33/582 , G01N33/585
摘要： The present invention provides a staining method in which the fluorescent staining properties in a fluorescently-immunostained specimen are not reduced even when an oil-based mounting medium is used. The present invention also provides a method of preventing deterioration of a fluorescent label caused by irradiation with excitation light and improving the light resistance in a fluorescently-immunostained specimen obtained by the staining method. The biological substance detection method according to the present invention is a biological substance detection method for specifically detecting a biological substance from a pathological specimen, which includes the steps of: immunostaining the specimen with a fluorescent label; immobilizing the thus stained specimen; and mounting the thus immobilized specimen using a mounting medium including an organic solvent not freely miscible with water. In the biological substance detection method, the above-described mounting medium further includes a discoloration inhibitor.
摘要翻译：本发明提供了即使使用油性安装介质时，荧光免疫染色试样中的荧光染色性也不会降低的染色方法。本发明还提供一种防止由激发光照射引起的荧光标记物的劣化并提高通过染色方法获得的荧光免疫染色试样中的耐光性的方法。本发明的生物体检测方法是从病理标本中特异性检测生物体的生物物质检测方法，其特征在于，包括以下步骤：用荧光标记物免疫染色标本; 固定如此染色的样品; 并使用包括不能与水自由混溶的有机溶剂的安装介质来安装如此固定的样品。在生物物质检测方法中，上述安装介质还包括变色抑制剂。

4. 发明申请

US20150049936A1 MEDICAL IMAGE PROCESSOR AND STORAGE MEDIUM 有权
标题翻译：医学图像处理器和存储介质
公开(公告)号：US20150049936A1
公开(公告)日：2015-02-19
申请号：US14387405
申请日：2013-03-27
申请人： Konica Minolta, Inc. , Tohoku University
发明人： Akinori Tsunomori , Hisatake Okada , Kohsuke Gonda , Noriaki Ohuchi , Mika Watanabe
IPC分类号： G06K9/00 , G06T7/00 , G06K9/46
CPC分类号： G06K9/0014 , G01N21/6456 , G01N21/6458 , G06K9/4661 , G06T7/0012 , G06T2207/10064 , G06T2207/20021 , G06T2207/20104 , G06T2207/20221 , G06T2207/30024 , G06T2207/30096
摘要： A medical image processor and storage medium are shown. According to one implementation, a medical image processor includes an input unit, an operation unit, a cell nucleus extracting unit, a fluorescent bright point extracting unit, a feature amount calculating unit, and an output unit. The input unit is used to input a cell shape image showing a shape of a cell and a fluorescent image showing expression of a specific protein as a fluorescent bright point. The operation unit is used to specify an analysis target region. The cell nucleus extracting unit extracts a region of a cell nucleus. The fluorescent bright point extracting unit extracts a fluorescent bright point. The feature amount calculating unit calculates a feature amount showing an expression amount of the specific protein. The output unit outputs the calculated feature amount.
摘要翻译：显示医疗图像处理器和存储介质。根据一个实施方式，医用图像处理器包括输入单元，操作单元，单元核提取单元，荧光亮点提取单元，特征量计算单元和输出单元。输入单元用于输入显示细胞形状的细胞形状图像和显示特定蛋白质的表达的荧光图像作为荧光亮点。操作单元用于指定分析目标区域。细胞核提取单元提取细胞核的区域。荧光亮点提取单元提取荧光亮点。特征量计算单元计算显示特定蛋白质的表达量的特征量。输出单元输出计算出的特征量。

5. 发明授权

US11435348B2 Tissue staining method 有权
公开(公告)号：US11435348B2
公开(公告)日：2022-09-06
申请号：US16715180
申请日：2019-12-16
申请人： Konica Minolta, Inc.
发明人： Hideki Gouda , Hisatake Okada , Yasushi Nakano , Kohsuke Gonda , Noriaki Ohuchi , Mika Watanabe
IPC分类号： G01N33/569 , G01N1/30
摘要： It is an object of the present invention to provide a tissue staining method that makes it possible to observe both information on the morphology of a tissue and information on a biological substance such as an antigen molecule to be detected on a single section and in a single view field. The present invention provides a tissue staining method, including carrying out (A) a HE (hematoxylin-eosin) staining, and (B) a histochemical staining, serially on a single tissue section, wherein the histochemical staining is defined as a histochemical technique for detecting a biological substance to be detected in a tissue in a visible manner by use of a binding reaction between the biological substance to be detected and a probe biological substance capable of binding specifically to the biological substance to be detected.

6. 发明授权

US11275087B2 Test support method for supporting prediction of pathological complete response (pCR) using fluorescent nanoparticles 有权
公开(公告)号：US11275087B2
公开(公告)日：2022-03-15
申请号：US15806970
申请日：2017-11-08
申请人： Konica Minolta, Inc.
发明人： Hideki Gouda , Masaru Takahashi , Kensaku Takanashi , Hisatake Okada , Yuichi Ozaki , Yuka Yoshihara , Yasushi Nakano , Kohsuke Gonda , Noriaki Ohuchi , Mika Watanabe , Norikazu Masuda , Masakazu Toi , Hiroshi Tada , Minoru Miyashita
IPC分类号： G01N33/574 , G01N33/52 , G01N33/53 , G06K9/00 , G01N33/58 , G01N1/30 , G01N33/50
摘要： The present invention relates to a test support method which is a means for predicting whether or not pathological complete response (pCR) will be attained when a preoperative chemotherapy with an anticancer drug is performed, the method using a sample (breast cancer tissue section) collected from a breast cancer patient to be subjected to the preoperative chemotherapy. The test support method includes: [1] the step of acquiring a fluorescence image of a breast cancer tissue section, which fluorescence image shows bright spots of fluorescent nanoparticles labeling one or more kinds of breast cancer-related proteins; [2] the step of acquiring at least one index relating to the expression level(s) of the breast cancer-related protein(s) on the basis of the bright spots of the fluorescence image; and [3] the step of acquiring information for predicting pCR by performing an analysis using the above-described at least one index.

7. 发明授权

US10746740B2 Biological substance quantitation method, pathological diagnosis support system, and recording medium storing computer readable program 有权
公开(公告)号：US10746740B2
公开(公告)日：2020-08-18
申请号：US15544795
申请日：2016-01-15
申请人： KONICA MINOLTA, INC.
发明人： Takeshi Isoda , Naoko Furusawa , Yasuyuki Motokui , Kohsuke Gonda , Noriaki Ohuchi , Mika Watanabe
IPC分类号： G01N33/536 , G01N33/48 , G01N33/58 , G01N33/68
摘要： There is provided a biological substance quantitation method of quantitating a biological substance in a sample stained with a staining reagent including a fluorescent particle encapsulating a fluorescent substance, based on a fluorescence of the fluorescent substance. The method includes inputting a fluorescent image representing expression of the biological substance in the sample by a fluorescent bright spot; and quantitating an expression amount of the biological substance based on a fluorescence of the fluorescent bright spot. The biological substance is a nucleoprotein expressed at a cell nucleus. The fluorescent particle binds to the biological substance through a primary antibody which is directed against the biological substance as an antigen.

8. 发明授权

US10309900B1 Biological substance quantitation method, pathological diagnosis support system and recording medium storing computer readable program 有权
公开(公告)号：US10309900B1
公开(公告)日：2019-06-04
申请号：US16256224
申请日：2019-01-24
申请人： Konica Minolta, Inc.
发明人： Hideki Gouda , Kensaku Takanashi , Kohsuke Gonda , Noriaki Ohuchi , Mika Watanabe
IPC分类号： H04N7/18 , G06K9/00 , G01N21/64 , G01N33/483 , G01N33/58 , G06T7/90 , G01N21/93 , G06T7/00 , G01N33/53 , G01N33/48 , G01N33/50
摘要： A biological substance quantitation method includes the following. A fluorescent image is input, which represents an expression of a specific biological substance in a sample stained with a fluorescent substance by a florescent bright spot. A quantitative evaluation value of the florescent bright spot is calculated. A standard fluorescent image of a standard sample stained under a same condition as the sample and representing an expression of the biological substance in a standard sample, is input under a same condition as the fluorescent image. A quantitative evaluation value in the standard fluorescent image is calculated under a same condition as the fluorescent image. Based on a correlation between an expression amount of the biological substance in a standard sample measured in advance and the evaluation value in the standard fluorescent image, the evaluation value in the fluorescent image is converted to an expression amount of the biological substance in the sample.

9. 发明授权

US09483684B2 Medical image processor and storage medium 有权
标题翻译：医用图像处理器和存储介质
公开(公告)号：US09483684B2
公开(公告)日：2016-11-01
申请号：US14387405
申请日：2013-03-27
申请人： Konica Minolta, Inc. , Tohoku University
发明人： Akinori Tsunomori , Hisatake Okada , Kohsuke Gonda , Noriaki Ohuchi , Mika Watanabe
IPC分类号： G06K9/00 , G01N21/64 , G06K9/46 , G06T7/00
CPC分类号： G06K9/0014 , G01N21/6456 , G01N21/6458 , G06K9/4661 , G06T7/0012 , G06T2207/10064 , G06T2207/20021 , G06T2207/20104 , G06T2207/20221 , G06T2207/30024 , G06T2207/30096
摘要： A medical image processor and storage medium are shown. According to one implementation, a medical image processor includes an input unit, an operation unit, a cell nucleus extracting unit, a fluorescent bright point extracting unit, a feature amount calculating unit, and an output unit. The input unit is used to input a cell shape image showing a shape of a cell and a fluorescent image showing expression of a specific protein as a fluorescent bright point. The operation unit is used to specify an analysis target region. The cell nucleus extracting unit extracts a region of a cell nucleus. The fluorescent bright point extracting unit extracts a fluorescent bright point. The feature amount calculating unit calculates a feature amount showing an expression amount of the specific protein. The output unit outputs the calculated feature amount.
摘要翻译：显示医疗图像处理器和存储介质。根据一个实施方式，医用图像处理器包括输入单元，操作单元，单元核提取单元，荧光亮点提取单元，特征量计算单元和输出单元。输入单元用于输入显示细胞形状的细胞形状图像和显示特定蛋白质的表达的荧光图像作为荧光亮点。操作单元用于指定分析目标区域。细胞核提取单元提取细胞核的区域。荧光亮点提取单元提取荧光亮点。特征量计算单元计算显示特定蛋白质的表达量的特征量。输出单元输出计算出的特征量。

10. 发明授权

US10502728B2 Image processing device, pathological diagnosis support system, storage medium for image processing, and image processing method 有权
公开(公告)号：US10502728B2
公开(公告)日：2019-12-10
申请号：US16207378
申请日：2018-12-03
申请人： Konica Minolta, Inc.
发明人： Yasuhiro Watanabe , Shuji Ichitani , Kohsuke Gonda , Noriaki Ohuchi , Mika Watanabe
IPC分类号： G06K9/00 , H04N7/18 , G01N33/483 , G06T5/50 , G06T7/11 , A61B5/00 , G06T7/00
CPC分类号： G01N33/4833 , A61B5/0071 , G06K9/0014 , G06T5/50 , G06T7/0012 , G06T7/11 , G06T2207/10064 , G06T2207/20221 , G06T2207/30024
摘要： An image processing device (2A) comprises: an input means for inputting a brightfield image representing cell morphology in a tissue section, and a fluorescence image representing, by fluorescent bright spots, the expression of a specific protein in the same range of the tissue section; a first generation means for generating a cell image obtained by extracting a specific site of a cell from the brightfield image; a second generation means for generating an image obtained by extracting bright spot regions from the fluorescence image, creating a brightness profile for each bright spot region, and generating a fluorescent particle image obtained by extracting the fluorescent particles in the bright spot regions on the basis of the fluorescence profile for one fluorescent particle, which serves as a fluorescence bright spot source; and a calculation means for superimposing the cell image and the fluorescent particle image on one another.

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