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1. 发明授权

US08598541B2 Fluorescent image obtaining device, fluorescent image obtaining method and fluorescent image obtaining program 有权
标题翻译：荧光图像获取装置，荧光图像获取方法和荧光图像获取程序
公开(公告)号：US08598541B2
公开(公告)日：2013-12-03
申请号：US12868187
申请日：2010-08-25
申请人： Koichiro Kishima , Yu Hirono , Nobuhiro Hayashi , Takamichi Yamakoshi , Nobuhiro Kihara , Takashi Yamamoto , Fumiyasu Suzuki , Ryu Narusawa
发明人： Koichiro Kishima , Yu Hirono , Nobuhiro Hayashi , Takamichi Yamakoshi , Nobuhiro Kihara , Takashi Yamamoto , Fumiyasu Suzuki , Ryu Narusawa
IPC分类号： G01J1/58
CPC分类号： G01N21/6458 , G01N21/6428
摘要： A fluorescent image obtaining device includes a light source that irradiates light such that a fluorescent material marked on a target in a biological sample lies in a non-excited state and a fluorescent material marked on a control with the target lies in an excited state, and an imaging unit that takes an image including the entire biological sample.
摘要翻译：荧光图像获取装置包括照射光的光源，使得标记在生物样品中的靶上的荧光物质处于非激发状态，并且标记在靶上的荧光材料处于激发状态，并且拍摄包含整个生物样品的图像的成像单元。

2. 发明授权

US08421033B2 Fluorescence image producing method, fluorescence image producing apparatus, and fluorescence image producing program 有权
标题翻译：荧光图像产生方法，荧光图像产生装置和荧光图像产生程序
公开(公告)号：US08421033B2
公开(公告)日：2013-04-16
申请号：US12851748
申请日：2010-08-06
申请人： Koichiro Kishima , Takashi Yamamoto
发明人： Koichiro Kishima , Takashi Yamamoto
IPC分类号： G01N21/64
CPC分类号： A45D33/02 , G01J1/58 , G01N21/6458 , G01N2021/6419 , G01N2021/6421 , G01N2021/6441 , G02B21/006 , G02B21/0076 , G02B21/10 , G02B21/16 , G02B21/244 , G02B21/367
摘要： Method and apparatus for producing an image associated with a biological sample is disclosed. The biological sample is focused on the biological sample based on fluorescence of a first fluorescent material and the image is captured based on fluorescence of the second fluorescent material. A computer readable memory device storing instructions to cause a data processing unit is also disclosed.
摘要翻译：公开了用于生产与生物样品相关联的图像的方法和装置。生物样品基于第一荧光材料的荧光聚焦在生物样品上，并且基于第二荧光材料的荧光捕获图像。还公开了存储引起数据处理单元的指令的计算机可读存储器件。

3. 发明申请

US20110049386A1 FLUORESCENCE IMAGE PRODUCING METHOD, FLUORESCENCE IMAGE PRODUCING APPARATUS, AND FLUORESCENCE IMAGE PRODUCING PROGRAM 有权
标题翻译：荧光图像生产方法，荧光图像生产设备和荧光图像生产程序
公开(公告)号：US20110049386A1
公开(公告)日：2011-03-03
申请号：US12851748
申请日：2010-08-06
申请人： Koichiro Kishima , Takashi Yamamoto
发明人： Koichiro Kishima , Takashi Yamamoto
IPC分类号： G01J1/58 , G06K9/00
CPC分类号： A45D33/02 , G01J1/58 , G01N21/6458 , G01N2021/6419 , G01N2021/6421 , G01N2021/6441 , G02B21/006 , G02B21/0076 , G02B21/10 , G02B21/16 , G02B21/244 , G02B21/367
摘要： Method and apparatus for producing an image associated with a biological sample is provided. The biological sample is focused on the biological sample based on fluorescence of a first fluorescent material and the image is captured based on fluorescence of the second fluorescent material. A computer readable memory device storing instructions to cause a data processing unit is also provided.
摘要翻译：提供了用于生产与生物样品相关联的图像的方法和装置。生物样品基于第一荧光材料的荧光聚焦在生物样品上，并且基于第二荧光材料的荧光捕获图像。还提供了存储用于引起数据处理单元的指令的计算机可读存储器件。

4. 发明申请

US20110157349A1 STAGE CONTROL DEVICE, STAGE CONTROL METHOD, STAGE CONTROL PROGRAM, AND MICROSCOPE 审中-公开
标题翻译：阶段控制装置，阶段控制方法，阶段控制程序和显微镜
公开(公告)号：US20110157349A1
公开(公告)日：2011-06-30
申请号：US12972030
申请日：2010-12-17
申请人： Takashi Yamamoto , Koichiro Kishima , Ryu Narusawa , Yu Hirono , Fumiyasu Suzuki
发明人： Takashi Yamamoto , Koichiro Kishima , Ryu Narusawa , Yu Hirono , Fumiyasu Suzuki
IPC分类号： H04N7/18 , H04N5/225
CPC分类号： G02B21/26 , G01Q30/20 , G02B21/006 , G02B21/14 , G02B21/24 , G02B21/36 , G02B27/0075
摘要： A stage control device is provided and includes: an obtaining section obtaining a set of images as different visual points in all or a part of a sample set as a photographing object; a distance calculating section calculating a distance between each pixel of one image to be set as a reference in the set of images and a relative pixel in the other image; a tilt angle calculating section calculating a tilt angle of the sample using the distance calculated by the distance calculating section; and an adjusting section adjusting a tilt angle of a stage surface on which the sample is disposed so as to minimize a tilt within an angle of view of a photographed picture on a basis of the tilt angle of the sample.
摘要翻译：提供了一种舞台控制装置，包括：获取部，其在作为拍摄对象的样本集的全部或一部分中获得作为不同视点的一组图像; 距离计算部分，计算要在图像集合中设置为参考的一个图像的每个像素与另一图像中的相对像素之间的距离; 倾斜角计算部，使用由所述距离计算部计算出的距离来计算所述样本的倾斜角度; 以及调整部，其调整配置有所述样本的台面的倾斜角度，以便根据所述样品的倾斜角度使拍摄图像的视角内的倾斜最小化。

5. 发明授权

US09110306B2 Tissue-slice image acquirement and display apparatus, tissue-slice image acquirement and display method, and tissue-slice image acquirement and display program 有权
标题翻译：组织切片图像获取和显示装置，组织切片图像获取和显示方法以及组织切片图像获取和显示程序
公开(公告)号：US09110306B2
公开(公告)日：2015-08-18
申请号：US12862220
申请日：2010-08-24
申请人： Nobuhiro Hayashi , Koichiro Kishima , Takashi Yamamoto , Nobuhiro Kihara , Takamichi Yamakoshi , Fumiyasu Suzuki , Ryu Narusawa
发明人： Nobuhiro Hayashi , Koichiro Kishima , Takashi Yamamoto , Nobuhiro Kihara , Takamichi Yamakoshi , Fumiyasu Suzuki , Ryu Narusawa
IPC分类号： G06K9/00 , G02B21/36 , G06K9/32 , G06T7/00 , G01N1/31 , G02B21/34
CPC分类号： G02B21/367 , G01N1/312 , G02B21/34 , G06K9/00134 , G06K9/3216 , G06K9/3233 , G06T7/11 , G06T7/174 , G06T2207/10056 , G06T2207/30024
摘要： A tissue-slice image acquirement and display apparatus includes: an entire-image acquirer that acquires a bright-field image of an entirety of a first slice of a tissue and a dark-field image of an entirety of a second slice of the tissue; a modifier that modifies a profile shape of a slice portion in the dark-field image on the basis of a profile shape of a slice portion in the bright-field image; a magnified-image acquirer that determines a range encompassing the second slice on the basis of the profile shape of the modified slice portion and that acquires, in a dark field, a magnified image of the second slice in the determined range; and a display controller that causes the bright-field image to be displayed and that causes a portion of the magnified image, the portion corresponding to a position selected in the bright-field image, to be displayed.
摘要翻译：组织切片图像获取和显示装置包括：整体图像获取器，其获取组织的第一切片的整体的亮场图像和组织的第二切片的整体的暗视场图像; 基于亮场图像中的切片部分的轮廓形状来修改暗视场图像中的切片部分的轮廓形状的修改器; 放大图像获取器，其基于修改的切片部分的轮廓形状确定包围第二切片的范围，并且在暗场中获取在所确定的范围内的第二切片的放大图像; 以及显示控制器，其使亮域图像被显示，并且使得显示放大图像的一部分，与亮域图像中选择的位置对应的部分。

6. 发明申请

US20110049389A1 FLUORESCENT IMAGE OBTAINING DEVICE, FLUORESCENT IMAGE OBTAINING METHOD AND FLUORESCENT IMAGE OBTAINING PROGRAM 有权
标题翻译：荧光图像获取装置，荧光图像获取方法和荧光图像获取程序
公开(公告)号：US20110049389A1
公开(公告)日：2011-03-03
申请号：US12868187
申请日：2010-08-25
申请人： Koichiro Kishima , Yu Hirono , Nobuhiro Hayashi , Takamichi Yamakoshi , Nobuhiro Kihara , Takashi Yamamoto , Fumiyasu Suzuki , Ryu Narusawa
发明人： Koichiro Kishima , Yu Hirono , Nobuhiro Hayashi , Takamichi Yamakoshi , Nobuhiro Kihara , Takashi Yamamoto , Fumiyasu Suzuki , Ryu Narusawa
IPC分类号： G01J1/58
CPC分类号： G01N21/6458 , G01N21/6428
摘要： A fluorescent image obtaining device includes a light source that irradiates light such that a fluorescent material marked on a target in a biological sample lies in a non-excited state and a fluorescent material marked on a control with the target lies in an excited state, and an imaging unit that takes an image including the entire biological sample.
摘要翻译：荧光图像获取装置包括照射光的光源，使得标记在生物样品中的靶上的荧光物质处于非激发状态，并且标记在靶上的荧光材料处于激发状态，并且拍摄包含整个生物样品的图像的成像单元。

7. 发明授权

US10239106B2 Spinning method and spinning apparatus 有权
公开(公告)号：US10239106B2
公开(公告)日：2019-03-26
申请号：US14781151
申请日：2014-04-01
申请人： Yukitaka Kunimoto , Satoshi Shionoya , Takashi Yamamoto
发明人： Yukitaka Kunimoto , Satoshi Shionoya , Takashi Yamamoto
IPC分类号： B21D22/14 , B21D22/16 , B21D22/18
摘要： A spinning method includes supporting a supported portion of a cylindrical work by a work supporting portion; pressing a first roller of a spinning head against a processed portion of the work while revolving the first roller; and performing a forming process that points a tube axis of the processed portion of the work in a given direction by pressing the first roller and a second roller in which a plane of revolution thereof is provided in a different position, in a rotational axis direction of a spindle of the spinning head, than a plane of revolution of the first roller, while revolving the first roller and the second roller, and moving the work supporting portion relative to the spinning head or moving the spinning head relative to the work supporting portion, while the first roller and the second roller work in cooperation with each other to retain the work.

8. 发明授权

US09176311B2 Microscope control device and optical distortion correction method 有权
标题翻译：显微镜控制装置和光学畸变校正方法
公开(公告)号：US09176311B2
公开(公告)日：2015-11-03
申请号：US13192803
申请日：2011-07-28
申请人： Takashi Yamamoto
发明人： Takashi Yamamoto
IPC分类号： H04N7/18 , G02B21/14
CPC分类号： G02B21/14
摘要： A microscope control device includes an offset processing unit that corrects phase contrast information regarding a phase contrast between corresponding phase contrast images, which is generated based on a set of the phase contrast images of a sample which are captured by a microscope, based on offset information of phase contrasts caused by optical distortions unique to the microscope, and a defocus amount calculation unit that calculates a defocus amount of the sample on the basis of the phase contrast information after offset correction.
摘要翻译：显微镜控制装置包括偏移处理单元，该偏移处理单元基于偏移信息校正基于由显微镜拍摄的样本的一组相位对比图像而生成的相应对比图像之间的相位对比度的相位对比度信息由显微镜独有的光学失真引起的相位对比度，以及散焦量计算单元，其基于偏移校正后的相位差信息来计算样本的散焦量。

9. 发明申请

US20150232877A1 METHODS AND COMPOSITIONS TO ENHANCE ACTIVITY OF CRY ENDOTOXINS 审中-公开
标题翻译：提高内毒素活性的方法和组合
公开(公告)号：US20150232877A1
公开(公告)日：2015-08-20
申请号：US14435381
申请日：2013-10-14
申请人： Ruth CONG , Takashi YAMAMOTO , Zheng YI , PIONEER HI-BRED INTERNATIONAL, INC.
发明人： Ruth Cong , Takashi Yamamoto , Yi Zheng
IPC分类号： C12N15/82 , C07K14/245 , C07K14/325
CPC分类号： C12N15/8286 , C07K14/245 , C07K14/325 , C07K2319/20 , C07K2319/35 , C07K2319/55 , Y02A40/162
摘要： Methods and compositions for enhancing the resistance of plants to plant pests are provided. Chimeric pesticidal polypeptides and nucleic acid molecules encoding the chimeric pesticidal polypeptides are provided. The chimeric pesticidal polypeptides comprising a solubility-enhancing polypeptide operably linked to a polypeptide comprising pesticidal activity. The nucleic acid molecules can be used in expression cassettes for making transformed plants with enhanced resistance to plant pests. Further provided are transformed plants, plant tissues, plant cells, other host cells, and seeds as well as pesticidal compositions.
摘要翻译：提供了用于增强植物对植物害虫的抗性的方法和组合物。提供嵌合农药多肽和编码嵌合杀虫多肽的核酸分子。所述嵌合杀虫多肽包含可操作地连接于包含杀虫活性的多肽的溶解度增强多肽。核酸分子可用于表达盒中，用于制备对植物害虫具有增强抗性的转化植物。进一步提供了转化植物，植物组织，植物细胞，其他宿主细胞和种子以及杀虫组合物。

10. 发明授权

US09109231B2 Synthetic insecticidal proteins active against corn rootworm 有权
标题翻译：对玉米根虫有活性的合成杀虫蛋白
公开(公告)号：US09109231B2
公开(公告)日：2015-08-18
申请号：US13370408
申请日：2012-02-10
申请人： Ericka Bermudez , Ruth Cong , Jingtong Hou , Takashi Yamamoto
发明人： Ericka Bermudez , Ruth Cong , Jingtong Hou , Takashi Yamamoto
IPC分类号： C12N15/82 , C07K14/325 , A01N37/46 , A01N63/02
CPC分类号： C12N15/8286 , A01N37/46 , A01N63/02 , C07K14/325 , Y02A40/162
摘要： Traditionally, the primary method for impacting insect pest populations is the application of broad-spectrum chemical insecticides. However, there is increasing concern about the environmental hazards associated with the production and use of synthetic chemical pesticides. Thus, there is substantial interest in developing alternative pesticides, including biological control of insect pests of agricultural significance using a microbial agent or another species of insect. The present invention provides compositions and methods for such biological control. Modified Cry3 pesticidal polypeptides, polynucleotides encoding such polypeptides, and methods of use are disclosed. The modified polynucleotides provided herein can be used to transform organisms and cells of hosts comprising plant, insects, and microorganisms. The expression of modified polypeptides can provide the host with improved insecticidal activity against one or more insect pathogens.
摘要翻译：传统上，影响昆虫害虫种群的主要方法是应用广谱化学杀虫剂。然而，对生产和使用合成化学农药的环境危害越来越受到关注。因此，使用微生物剂或其他昆虫种类开发替代农药，包括对具有农业意义的害虫的生物防治具有重大意义。本发明提供了用于这种生物学控制的组合物和方法。公开了修饰的Cry3杀虫多肽，编码这些多肽的多核苷酸和使用方法。本文提供的修饰的多核苷酸可用于转化包含植物，昆虫和微生物的宿主的生物体和细胞。修饰的多肽的表达可以为宿主提供对一种或多种昆虫病原体的改善的杀虫活性。

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