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1. 发明申请

US20130230866A1 Method for Determining Effectiveness of Medicine Containing Antibody as Component 审中-公开
标题翻译：测定含有抗体作为成分的药物有效性的方法
公开(公告)号：US20130230866A1
公开(公告)日：2013-09-05
申请号：US13822320
申请日：2011-08-26
申请人： Minoru Miyashita , Kohsuke Gonda , Noriaki Ohuchi , Motohiro Takeda
发明人： Minoru Miyashita , Kohsuke Gonda , Noriaki Ohuchi , Motohiro Takeda
IPC分类号： G01N21/64
CPC分类号： G01N21/6486 , G01N21/6428 , G01N21/6456 , G01N33/582 , G01N33/6854 , G01N33/94 , G01N2800/52
摘要： Protein recognized by an antibody used as an active ingredient of an antibody medicine such as trastuzumab or an antibody used for targeting a target site of an active ingredient is highly accurately quantitatively determined by employing a quantitative tissue staining method of biological tissues, thereby providing a method for determining therapeutic effectiveness of a medicine containing such an antibody as a component. The effectiveness of a medicine containing an antibody as a component is determined by employing a tissue staining method comprising the steps of: labeling the antibody in the medicine containing an antibody as a component with a fluorescent material and contacting the thus fluorescence-labeled antibody with a tissue sample; obtaining a fluorescence image by irradiating, with excitation light, a tissue site contacted with the antibody; obtaining an autofluorescence image in the same field of view and at the same focus as in the fluorescence image in a close region on a shorter wavelength side or a longer wavelength side of an acquisition wavelength region of fluorescence emitted by the fluorescent material; obtaining a corrected fluorescence image by performing image processing for removing fluorescence brightness of the autofluorescence image from fluorescence brightness of the fluorescence image; counting the number of cells in the tissue site contacted with the antibody; measuring average fluorescence brightness per fluorescent particle; and calculating the number of fluorescent particles per cell.
摘要翻译：通过使用生物组织的定量组织染色法，高度准确地定量地测定由用作抗体药物如曲妥珠单抗或用于靶向活性成分的靶位点的抗体的抗体识别的蛋白质，从而提供方法用于确定含有作为组分的抗体的药物的治疗有效性。含有抗体作为成分的药物的有效性通过使用组织染色法测定，该方法包括以下步骤：在含有抗体作为组分的药物中用荧光物质标记抗体，并将由此荧光标记的抗体与组织样本通过用激发光照射与抗体接触的组织部位来获得荧光图像; 在荧光材料发射的荧光的采集波长区域的较短波长侧或较长波长侧的接近区域中，以与荧光图像相同的视场和相同的焦点获得自发荧光图像; 通过进行从荧光图像的荧光亮度去除自发荧光图像的荧光亮度的图像处理来获得校正的荧光图像; 计数与抗体接触的组织部位中的细胞数; 测量每个荧光颗粒的平均荧光亮度; 并计算每个细胞的荧光颗粒数。

2. 发明申请

US20130203082A1 METHOD FOR DETERMINING CANCER ONSET OR CANCER ONSET RISK 审中-公开
标题翻译：用于确定癌症或癌症风险的方法
公开(公告)号：US20130203082A1
公开(公告)日：2013-08-08
申请号：US13819008
申请日：2011-08-26
申请人： Kohsuke Gonda , Minoru Miyashita , Motohiro Takeda , Noriaki Ohuchi
发明人： Kohsuke Gonda , Minoru Miyashita , Motohiro Takeda , Noriaki Ohuchi
IPC分类号： G01N21/64
CPC分类号： G01N21/6486 , B82Y15/00 , G01N21/6428 , G01N21/6458 , G01N33/57415 , G01N33/588 , G01N2800/50
摘要： A highly accurate and quantitative method for determining cancer onset or cancer onset risk by a quantitative tissue staining method in biological tissues using an antibody capable of recognizing a cancer growth regulatory factor or cancer metastasis regulatory factor such as PAR1 antibody, which inhibits the cancer cell mobility and infiltration is provided. Cancer onset or cancer onset risk is determined using the tissue staining method comprising the steps of: labeling an antibody which recognizes a cancer growth regulatory factor or cancer metastasis regulatory factor with a fluorescent material, and contacting the fluorescent-labeled antibody with a tissue sample; irradiating a tissue site in contact with the antibody with excitation light to acquire a fluorescence image; acquiring an autofluorescence image in a vicinity region of a short wavelength side or long wavelength side of an acquisition region of fluorescence wavelength emitted by the fluorescent material, in the same field of vision and in the same focal point as those of the fluorescence image; acquiring a corrected fluorescence image by image processing to eliminate a fluorescent brightness of the autofluorescence image from the fluorescent brightness of the fluorescence image; counting the number of cells at the tissue site in contact with the antibody; measuring a mean fluorescent brightness of a single fluorescent particle; and calculating the number of fluorescent particles per cell.
摘要翻译：使用能够识别癌细胞生长调节因子或癌转移调节因子（例如抑制癌细胞迁移率的PAR1抗体）的抗体，通过定量组织染色法在生物组织中测定癌症发病或癌症发病风险的高度准确和定量的方法并提供渗透。使用组织染色方法确定癌症发病或癌症发病风险，其包括以下步骤：用荧光材料标记识别癌症生长调节因子或癌症转移调节因子的抗体，并将荧光标记的抗体与组织样品接触; 用激发光照射与抗体接触的组织部位以获得荧光图像; 在荧光材料发射的荧光波长的获取区域的短波长侧或长波长侧的附近区域中，在与荧光图像相同的视场和相同的焦点中获取自发荧光图像; 通过图像处理获得校正的荧光图像，以从荧光图像的荧光亮度消除自发荧光图像的荧光亮度; 计数与抗体接触的组织部位的细胞数; 测量单个荧光颗粒的平均荧光亮度; 并计算每个细胞的荧光颗粒数。

3. 发明申请

US20130157895A1 TISSUE STAINING METHOD, TISSUE EVALUATION METHOD AND BIOSUBSTANCE DETECTION METHOD 审中-公开
公开(公告)号：US20130157895A1
公开(公告)日：2013-06-20
申请号：US13819574
申请日：2011-03-15
申请人： Takuji Aimiya , Hideki Gouda , Hisatake Okada , Yasushi Nakano , Kohsuke Gonda , Motohiro Takeda , Noriaki Ohuchi
发明人： Takuji Aimiya , Hideki Gouda , Hisatake Okada , Yasushi Nakano , Kohsuke Gonda , Motohiro Takeda , Noriaki Ohuchi
IPC分类号： G01N1/30 , G01N33/74
摘要： A tissue staining method which comprises: staining a tissue with a staining reagent wherein a biosubstance recognition site is bonded to particles carrying multiple fluorescent substances accumulated therein; in the stained tissue, counting fluorescent points or measuring fluorescent brightness; and evaluating the expression level of a biosubstance, which matches the biosubstance recognition site, in the aforesaid tissue on the basis of the number of the fluorescent points or fluorescent brightness that was measured.

4. 发明授权

US10809167B2 Tissue staining method with staining agent containing particle holding plural phosphors 有权
公开(公告)号：US10809167B2
公开(公告)日：2020-10-20
申请号：US13819574
申请日：2011-03-15
申请人： Takuji Aimiya , Hideki Gouda , Hisatake Okada , Yasushi Nakano , Kohsuke Gonda , Motohiro Takeda , Noriaki Ohuchi
发明人： Takuji Aimiya , Hideki Gouda , Hisatake Okada , Yasushi Nakano , Kohsuke Gonda , Motohiro Takeda , Noriaki Ohuchi
IPC分类号： G01N1/30 , B82Y30/00 , B82Y15/00 , G01N33/58 , G01N21/64 , G01N33/74
摘要： A tissue staining method which comprises: staining a tissue with a staining reagent wherein a biosubstance recognition site is bonded to particles carrying multiple fluorescent substances accumulated therein; in the stained tissue, counting fluorescent points or measuring fluorescent brightness; and evaluating the expression level of a biosubstance, which matches the biosubstance recognition site, in the aforesaid tissue on the basis of the number of the fluorescent points or fluorescent brightness that was measured.

5. 发明授权

US08674079B2 Cancer cell migration and cancer cell invasion inhibitor 有权
标题翻译：癌细胞迁移和癌细胞侵袭抑制剂
公开(公告)号：US08674079B2
公开(公告)日：2014-03-18
申请号：US12934133
申请日：2009-03-19
申请人： Kohsuke Gonda , Hideo Higuchi , Noriaki Ohuchi , Motohiro Takeda
发明人： Kohsuke Gonda , Hideo Higuchi , Noriaki Ohuchi , Motohiro Takeda
IPC分类号： C07K16/00
CPC分类号： C07K16/28 , A61K2039/505 , C07K7/06 , C07K7/08 , C07K2317/34 , C07K2317/76 , G01N33/5029 , G01N33/574 , G01N2500/02 , G01N2500/10 , G01N2800/00
摘要： Provided are an antibody which binds specifically PAR1 (protease activated receptor 1) or a fragment of the antibody which retains similar characteristics thereto; a composition containing the same for inhibiting the migration activity and invasion activity of cancer cells; and a medicinal composition for treating cancer and the like.
摘要翻译：提供了特异性结合PAR1（蛋白酶活化受体1）或与其保持相似特征的抗体片段的抗体; 含有抑制癌细胞的迁移活性和侵袭活性的组合物; 以及用于治疗癌症等的药物组合物。

6. 发明申请

US20130157287A1 BIOLOGICAL SUBSTANCE DETECTION METHOD 有权
标题翻译：生物物质检测方法
公开(公告)号：US20130157287A1
公开(公告)日：2013-06-20
申请号：US13819453
申请日：2011-08-30
申请人： Kensaku Takanashi , Hideki Gouda , Hisatake Okada , Yasushi Nakano , Kohsuke Gonda , Motohiro Takeda , Noriaki Ohuchi
发明人： Kensaku Takanashi , Hideki Gouda , Hisatake Okada , Yasushi Nakano , Kohsuke Gonda , Motohiro Takeda , Noriaki Ohuchi
IPC分类号： G01N21/64
CPC分类号： G01N21/6486 , G01N21/6428 , G01N21/6458 , G01N33/4833 , G01N33/582 , G01N2021/6439 , G01N2021/6441
摘要： A biological substance detection method for detecting a biological substance specifically in a pathological specimen, comprising a step of immunologically staining the pathological specimen using a fluorescent label, a step of staining the pathological specimen with a staining reagent for morphology observation purposes (eosin) to observe the morphology of the pathological specimen, a step of irradiating the stained pathological specimen, with excited light to cause the emission of a fluorescent and detecting the biological substance in the pathological specimen. In the step of immunologically staining the pathological specimen, a special fluorescent particle for which the excitation wavelength appears in a region that is different from the excitation wavelength region of eosin is used as the fluorescent label.
摘要翻译：一种用于特异性检测病理标本中生物物质的生物物质检测方法，包括使用荧光标记对病理标本进行免疫染色的步骤，用形态学观察目的的染色试剂（曙红）染色病理标本的步骤，以观察病理标本的形态，用激发光照射染色的病理标本的步骤，引起荧光发射并检测病理标本中的生物物质。在免疫染色病理标本的步骤中，使用在与曙红的激发波长区域不同的区域中出现激发波长的特殊荧光粒子作为荧光标记。

7. 发明申请

US20110070163A1 CANCER CELL MIGRATION AND CANCER CELL INVASION INHIBITOR 有权
标题翻译：癌细胞移植和癌细胞侵入抑制剂
公开(公告)号：US20110070163A1
公开(公告)日：2011-03-24
申请号：US12934133
申请日：2009-03-19
申请人： Kohsuke Gonda , Hideo Higuchi , Noriaki Ohuchi , Motohiro Takeda
发明人： Kohsuke Gonda , Hideo Higuchi , Noriaki Ohuchi , Motohiro Takeda
IPC分类号： C07K16/28 , A61K39/395 , G01N33/574 , A61K49/00 , C07K7/06 , C07K17/02 , A61P35/00
CPC分类号： C07K16/28 , A61K2039/505 , C07K7/06 , C07K7/08 , C07K2317/34 , C07K2317/76 , G01N33/5029 , G01N33/574 , G01N2500/02 , G01N2500/10 , G01N2800/00
摘要： Provided are an antibody which binds specifically PAR1 (protease activated receptor 1) or a fragment of the antibody which retains similar characteristics thereto; a composition containing the same for inhibiting the migration activity and invasion activity of cancer cells; and a medicinal composition for treating cancer and the like.
摘要翻译：提供了特异性结合PAR1（蛋白酶活化受体1）或与其保持相似特征的抗体片段的抗体; 含有抑制癌细胞的迁移活性和侵袭活性的组合物; 以及用于治疗癌症等的药物组合物。

8. 发明授权

US08969100B2 Method for detecting afferent lymph vessel inflow regions and method for identifying specific cells 有权
标题翻译：检测传入淋巴管流入区域的方法和识别特定细胞的方法
公开(公告)号：US08969100B2
公开(公告)日：2015-03-03
申请号：US13379320
申请日：2010-03-11
申请人： Makoto Hikage , Kohsuke Gonda , Motohiro Takeda , Takashi Kamei , Noriaki Ohuchi , Hideki Gouda , Yasushi Nakano
发明人： Makoto Hikage , Kohsuke Gonda , Motohiro Takeda , Takashi Kamei , Noriaki Ohuchi , Hideki Gouda , Yasushi Nakano
IPC分类号： G01N33/543 , G01N33/574 , G01N21/64 , A61K49/00 , B82Y5/00 , B82Y15/00 , G01N33/58 , A61B5/00
CPC分类号： G01N21/6458 , A61B5/0071 , A61B5/41 , A61B5/418 , A61K49/0017 , A61K49/0067 , B82Y5/00 , B82Y15/00 , G01N33/57492 , G01N33/588 , G01N2201/0221 , Y10T436/13
摘要： Regions where metastatic cancer cells can exist are detected with high accuracy in a sentinel lymph node. Quantum dots are injected into the vicinity of a cancer in a living body, thereby identifying the location of the sentinel lymph node by means of fluorescence. Subsequently, the sentinel lymph node is extracted. With respect to the sentinel lymph node extracted with quantum dots injected, structural analysis is conducted by means of precision fluorescence measurement which uses a confocal fluorescence microscope for monomolecular observation. Specifically, the fluorescence intensity is measured with respect to each of multiple areas in the sentinel lymph nodes, and out of the multiple areas measured, one or more areas are detected as afferent lymph vessel inflow regions in descending order of fluorescence intensity.
摘要翻译：在前哨淋巴结中以高精度检测转移性癌细胞可存在的区域。将量子点注入生物体内的癌症附近，通过荧光鉴定前哨淋巴结的位置。随后，提取前哨淋巴结。对于注射量子点提取的前哨淋巴结，采用精细荧光测量法进行结构分析，采用共焦荧光显微镜进行单分子观察。具体地，针对前哨淋巴结中的多个区域中的每个区域测量荧光强度，并且在测量的多个区域中，以荧光强度的降序将一个或多个区域作为传入淋巴管流入区域检测。

9. 发明授权

US08901535B2 Semiconductor nanoparticle assembly 有权
标题翻译：半导体纳米颗粒组件
公开(公告)号：US08901535B2
公开(公告)日：2014-12-02
申请号：US13818849
申请日：2011-03-15
申请人： Masaru Takahashi , Kensaku Takanashi , Hideki Hoshino , Kohsuke Gonda , Motohiro Takeda , Noriaki Ohuchi
发明人： Masaru Takahashi , Kensaku Takanashi , Hideki Hoshino , Kohsuke Gonda , Motohiro Takeda , Noriaki Ohuchi
IPC分类号： H01L29/06 , H01L33/06 , C09K11/02 , C09K11/88 , C01B25/22
CPC分类号： H01L33/06 , C01B25/2212 , C09K11/02 , C09K11/025 , C09K11/88
摘要： A semiconductor nanoparticle assembly including semiconductor nanoparticles having a core/shell structure, and wherein the semiconductor nanoparticles are bonded by means of amide bonds.
摘要翻译：一种半导体纳米颗粒组件，包括具有核/壳结构的半导体纳米颗粒，并且其中半导体纳米颗粒通过酰胺键键合。

10. 发明申请

US20130153837A1 SEMICONDUCTOR NANOPARTICLE AGGREGATE AND PRODUCTION METHOD FOR SEMICONDUCTOR NANOPARTICLE AGGREGATE 审中-公开
标题翻译：半导体纳米粒子聚集体的半导体纳米聚集体和生产方法
公开(公告)号：US20130153837A1
公开(公告)日：2013-06-20
申请号：US13818804
申请日：2011-03-15
申请人： Hideki Hoshino , Masaru Takahashi , Kohsuke Gonda , Motohiro Takeda , Noriaki Ohuchi
发明人： Hideki Hoshino , Masaru Takahashi , Kohsuke Gonda , Motohiro Takeda , Noriaki Ohuchi
IPC分类号： C09K11/00 , C09K11/88 , C09K11/59 , C09K11/62 , C09K11/56 , C09K11/02
CPC分类号： C09K11/00 , A61K49/0067 , B01J13/04 , B82Y15/00 , B82Y30/00 , B82Y40/00 , C01B33/18 , C09K11/02 , C09K11/025 , C09K11/565 , C09K11/592 , C09K11/62 , C09K11/70 , C09K11/883 , G01N33/588 , H01B1/02
摘要： A semiconductor nanoparticle aggregate containing semiconductor nanoparticles with a core/shell structure is formed by controlling with physical energy the aggregation state of an agglomerate from agglomerated semiconductor nanoparticles.
摘要翻译：含有具有核/壳结构的半导体纳米颗粒的半导体纳米颗粒聚集体通过物理能量控制来自聚集的半导体纳米颗粒的聚集体的聚集状态而形成。

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