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1. 发明授权

US5403716A Method for measurement of tissue factor in high sensitivity and measurement kit therefor 失效
标题翻译：高灵敏度组织因子测量方法及其测量套件
公开(公告)号：US5403716A
公开(公告)日：1995-04-04
申请号：US924030
申请日：1992-09-03
申请人： Kimihiko Matsuzawa , Ryoichi Hasegawa
发明人： Kimihiko Matsuzawa , Ryoichi Hasegawa
IPC分类号： C07K16/36 , G01N33/86 , G01N33/544 , G01N33/535 , G01N33/551 , G01N33/577
CPC分类号： C07K16/36 , G01N33/86 , G01N2333/7454 , Y10S435/81 , Y10S436/826
摘要： The present invention provides an improved buffer solution for conducting an immunological method for measuring apoprotein of human tissue factor, and a kit therefor. More specifically, in a method for immunologically measuring the presence or concentration of apoprotein of human tissue factor contained in a sample, which method includes the steps of (1) forming in a buffer solution a ternary complex of (a) a first monoclonal antibody which is capable of specifically binding to the apoprotein and is immobilized on a solid carrier insoluble in the buffer solution, (b) the apoprotein and (c) a labeled second monoclonal antibody which is capable of specifically binding to the apoprotein at a different site from the binding site of the first monoclonal antibody, and (2) measuring the amount of labeled second monoclonal antibody in the ternary complex to determine the presence or concentration of apoprotein in the sample, the improvement of this invention comprising employing a buffer solution(i) containing in a concentration of 2 to 15 weight % at least one nonionic surfactant having an HLB value within a range of 12 to 30, and(ii) containing a protein having a molecular weight of about 16,000 to about 50,000 and an isoelectric point of 1.0 to 5.0.
摘要翻译： PCT No.PCT / JP92 / 00005 Sec。 371日期：1992年9月3日 102（e）1992年9月3日PCT PCT 1992年1月8日PCT公布。出版物WO92 / 日本1992年7月23日。本发明提供了一种改进的缓冲溶液，用于进行用于测量人组织因子的脱蛋白的免疫学方法及其试剂盒。更具体地，在用于免疫测定样品中包含的人组织因子的脱辅基蛋白的存在或浓度的方法中，所述方法包括以下步骤：（1）在缓冲溶液中形成（a）第一单克隆抗体的三元配合物，所述第一单克隆抗体能够特异性结合载脂蛋白并固定在不溶于缓冲溶液的固体载体上，（b）载脂蛋白和（c）标记的第二单克隆抗体，其能够与不同部位的脱辅基蛋白特异性结合第一单克隆抗体的结合位点，和（2）测量三元复合物中标记的第二单克隆抗体的量以确定样品中脱辅基蛋白的存在或浓度，本发明的改进包括使用缓冲溶液（i）浓度为2至15重量％的至少一种HLB值在12至30范围内的非离子表面活性剂，和（ii）含有蛋白质 n的分子量为约16,000至约50,000，等电点为1.0至5.0。

2. 发明授权

US4059571A Novel immunoglobulin derivatives and process for the preparation thereof 失效
标题翻译：新型免疫球蛋白衍生物及其制备方法
公开(公告)号：US4059571A
公开(公告)日：1977-11-22
申请号：US555132
申请日：1975-03-04
申请人： Katsuhiko Tomibe , Yasuhiko Masuho , Kimihiko Matsuzawa , Sachio Ishimoto , Kazuo Satake , Tsuneo Watanabe
发明人： Katsuhiko Tomibe , Yasuhiko Masuho , Kimihiko Matsuzawa , Sachio Ishimoto , Kazuo Satake , Tsuneo Watanabe
IPC分类号： C07K16/06 , A23J1/06
CPC分类号： C07K16/065
摘要： Novel immunoglobulin derivatives which are characterized in that in which the interchain disulfide bonds of immunoglobulin are predominantly cleaved, on the average 3 to 5 of the interchain disulfide bonds or the inter- and intra-chain disulfide bonds being cleaved, and so produced sulfur atoms (S-) are S-sulfonated (--S-SO.sub.3). The above immunoglobulin derivatives are prepared by reacting native immunoglobulin withA. a compound capable of forming tetrathionate ion, andB. a compound capable of forming in water, sulfite ion whereby cleaving on the average 3 to 5 inter-chain disulfide bonds, or inter- and intra-chain disulfide bonds, of the native immunoglobulin, and S-sulfonating (S-SO.sub.3) the so formed sulfur atoms. This invention also provides a water-soluble composition for injection containing said immunoglobulin derivative having the titre of anti-diphtheria of at least 1.0 I.U./ml at its concentration of 10.0% by weight, and a pharmaceutically-acceptable solubilizing agent for said immunoglobulin derivative.
摘要翻译：新型免疫球蛋白衍生物，其特征在于其中免疫球蛋白的链间二硫键主要被切割，平均3至5个链间二硫键或链内和链内二硫键被切割，并因此产生硫原子（ S-）是S-磺化的（-S-SO 3）。上述免疫球蛋白衍生物通过使天然免疫球蛋白与A.能够形成四硫酸根离子的化合物反应制备，和B.能够在水中形成的化合物，亚硫酸根离子，其中以平均3至5个链间二硫键切割， - 和链内二硫键，以及S-磺化（S-SO 3）如此形成的硫原子。本发明还提供一种含有所述免疫球蛋白衍生物的水溶性组合物，所述组合物的浓度为10.0重量％时具有至少1.0I./ml至少为1.0I./ml的滴度，以及用于所述免疫球蛋白衍生物的药学上可接受的增溶剂。

3. 发明授权

US4780205A Permselective hollow fiber membrane, process for the preparation thereof, method and apparatus for plasma components separation 失效
标题翻译：透视中空纤维膜，其制备方法，等离子体成分分离的方法和装置
公开(公告)号：US4780205A
公开(公告)日：1988-10-25
申请号：US887161
申请日：1986-06-30
申请人： Eiichi Murakami , Kimihiko Matsuzawa , Eiji Masunaga
发明人： Eiichi Murakami , Kimihiko Matsuzawa , Eiji Masunaga
IPC分类号： B01D67/00 , B01D69/02 , B01D69/08
CPC分类号： B01D67/0088 , B01D69/02 , B01D69/08
摘要： A permselective hollow fiber membrane which is a hollow fiber membrane, made from a wet spinnable organic high polymer, having a dense skin layer of gelated structure made to a thickness of 1.mu. or less on at least one surface of the membrane and the rest being made up into a porous layer, a process for the preparation thereof, a method and an apparatus of plasma components separation in which said hollow fiber membrane is used.
摘要翻译： PCT No.PCT / JP85 / 00602 Sec。 371日期1986年6月30日第 102（e）日期1986年6月30日PCT提交1985年10月30日PCT公布。出版物WO86 / 02575 日期：1986年5月9日。一种透湿中空纤维膜，其是由湿式可纺性有机高分子制成的中空纤维膜，其具有在至少一个表面上具有1μm以下厚度的凝胶化结构的致密表皮层膜和其余部分被制成多孔层，其制备方法，使用所述中空纤维膜的等离子体组分分离的方法和装置。

4. 发明授权

US5374533A Method for determining chondrocalcin 失效
标题翻译：测定软骨素的方法
公开(公告)号：US5374533A
公开(公告)日：1994-12-20
申请号：US51266
申请日：1993-04-23
申请人： Kimihiko Matsuzawa , Kazuhiko Itou , Hitomi Honda , Ryoichi Hasegwwa , Naomi Okamoto , Kenji Hosoda , Masayuki Shinmei , Shigeo Matsuyama
发明人： Kimihiko Matsuzawa , Kazuhiko Itou , Hitomi Honda , Ryoichi Hasegwwa , Naomi Okamoto , Kenji Hosoda , Masayuki Shinmei , Shigeo Matsuyama
IPC分类号： C07K16/18 , G01N33/68 , G01N33/53 , G01N33/543 , G01N33/553
CPC分类号： C07K16/18 , G01N33/6887 , G01N2800/105 , Y10S435/81 , Y10S436/811 , Y10S436/815
摘要： A method for determining chondrocalcin through an immunoassay using an enzyme(s)-labeled mammalian chondrocalcin or enzyme(s)-labeled anti-chondrocalcin antibody which is characterized by that mammalian body fluid is used as a specimen and immunoreaction in a solution is utilized to determine the chondrocalcin of the mammalian, reagents and a kit therefor.This determination can be utilized for diagnosis or the like for cartilage-relating diseases in mammals.
摘要翻译：通过使用酶标记的哺乳动物软骨素或酶标记的抗软骨素抗体通过免疫测定来测定软骨素的方法，其特征在于将哺乳动物体液用作样品，并将溶液中的免疫反应用于确定哺乳动物的软骨素，试剂及其试剂盒。该测定可以用于哺乳动物软骨相关疾病的诊断等。

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