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1. 发明授权

US4059571A Novel immunoglobulin derivatives and process for the preparation thereof 失效
标题翻译：新型免疫球蛋白衍生物及其制备方法
公开(公告)号：US4059571A
公开(公告)日：1977-11-22
申请号：US555132
申请日：1975-03-04
申请人： Katsuhiko Tomibe , Yasuhiko Masuho , Kimihiko Matsuzawa , Sachio Ishimoto , Kazuo Satake , Tsuneo Watanabe
发明人： Katsuhiko Tomibe , Yasuhiko Masuho , Kimihiko Matsuzawa , Sachio Ishimoto , Kazuo Satake , Tsuneo Watanabe
IPC分类号： C07K16/06 , A23J1/06
CPC分类号： C07K16/065
摘要： Novel immunoglobulin derivatives which are characterized in that in which the interchain disulfide bonds of immunoglobulin are predominantly cleaved, on the average 3 to 5 of the interchain disulfide bonds or the inter- and intra-chain disulfide bonds being cleaved, and so produced sulfur atoms (S-) are S-sulfonated (--S-SO.sub.3). The above immunoglobulin derivatives are prepared by reacting native immunoglobulin withA. a compound capable of forming tetrathionate ion, andB. a compound capable of forming in water, sulfite ion whereby cleaving on the average 3 to 5 inter-chain disulfide bonds, or inter- and intra-chain disulfide bonds, of the native immunoglobulin, and S-sulfonating (S-SO.sub.3) the so formed sulfur atoms. This invention also provides a water-soluble composition for injection containing said immunoglobulin derivative having the titre of anti-diphtheria of at least 1.0 I.U./ml at its concentration of 10.0% by weight, and a pharmaceutically-acceptable solubilizing agent for said immunoglobulin derivative.
摘要翻译：新型免疫球蛋白衍生物，其特征在于其中免疫球蛋白的链间二硫键主要被切割，平均3至5个链间二硫键或链内和链内二硫键被切割，并因此产生硫原子（ S-）是S-磺化的（-S-SO 3）。上述免疫球蛋白衍生物通过使天然免疫球蛋白与A.能够形成四硫酸根离子的化合物反应制备，和B.能够在水中形成的化合物，亚硫酸根离子，其中以平均3至5个链间二硫键切割， - 和链内二硫键，以及S-磺化（S-SO 3）如此形成的硫原子。本发明还提供一种含有所述免疫球蛋白衍生物的水溶性组合物，所述组合物的浓度为10.0重量％时具有至少1.0I./ml至少为1.0I./ml的滴度，以及用于所述免疫球蛋白衍生物的药学上可接受的增溶剂。

2. 发明授权

US5521097A Method of determining amino acid sequence of protein or peptide from carboxy-terminal 失效
标题翻译：从羧基末端测定蛋白质或肽的氨基酸序列的方法
公开(公告)号：US5521097A
公开(公告)日：1996-05-28
申请号：US931931
申请日：1992-08-18
申请人： Toyoaki Uchida , Akira Tsugita , Keiji Takamoto , Kazuo Satake
发明人： Toyoaki Uchida , Akira Tsugita , Keiji Takamoto , Kazuo Satake
IPC分类号： C07K1/12 , G01N33/68 , G01N33/00
CPC分类号： G01N33/6821 , C07K1/128 , Y10T436/24
摘要： A protein or peptide is treated by a vapor containing an organic acid represented by the following general formula CF.sub.3 --(CF.sub.2)n--COOH (n is zero or more integer). The resulting reaction mixture is processed by a mass spectrometer to obtain a mass spectrum to measure a mass of respective chemical species contained in the reaction mixture. Alternatively, the reaction mixture is processed by an amino acid analyzer to determine an amino acid sequence of the protein or peptide from the carboxy-terminal. According to another method, the protein or peptide is treated by an anhydride of the organic acid represented by the following general formula CF.sub.3 --(CF.sub.2)n--COOH (n is zero or more integer). The resulting reaction mixture is processed by a mass spectrometer to obtain a mass spectrum to measure a mass of respective chemical species contained in the reaction mixture to determine an amino acid sequence of the protein or peptide from the carboxy-terminal.
摘要翻译：通过含有由以下通式CF 3 - （CF 2）n -COOH表示的有机酸的蒸气处理蛋白质或肽（n为0以上整数）。通过质谱仪处理得到的反应混合物，得到质谱以测量反应混合物中各化学物质的质量。或者，通过氨基酸分析仪处理反应混合物以从羧基端确定蛋白质或肽的氨基酸序列。根据另一种方法，蛋白质或肽由下列通式CF 3 - （CF 2）n -COOH表示的有机酸的酸酐（n为0以上整数）处理。通过质谱仪处理所得反应混合物以获得质谱以测量反应混合物中所含的各化学物质的质量，以确定羧基末端的蛋白质或肽的氨基酸序列。

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