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1. 发明授权

US5556943A Cell adhesion protein, gene coding for the same, process for preparing the same and carrier onto which the same is immobilized 失效
标题翻译：细胞粘附蛋白，编码相同的基因，其制备方法和其上固定有载体的载体
公开(公告)号：US5556943A
公开(公告)日：1996-09-17
申请号：US328152
申请日：1994-10-24
申请人： Kenji Yamashita , Takaaki Ohara , Hideo Niwa , Fumio Osakada , Takeshi Fukuchi , Tadahiro Edamura , Tetsu Kakutani
发明人： Kenji Yamashita , Takaaki Ohara , Hideo Niwa , Fumio Osakada , Takeshi Fukuchi , Tadahiro Edamura , Tetsu Kakutani
IPC分类号： A61K38/00 , C07K14/705 , C12N1/21 , C12N15/12 , C07K14/47
CPC分类号： C07K14/70528 , A61K38/00
摘要： A sheep LFA-3 protein and derivertires thereof, and derivertives of a human LFA-3 protein, gene coding for the proteins, and processes for preparing the proteins. Said proteins have high affinity for human T-cells.
摘要翻译：绵羊LFA-3蛋白质及其衍生物，人LFA-3蛋白质的衍生物，编码蛋白质的基因，以及制备蛋白质的方法。所述蛋白质对人T细胞具有高亲和力。

2. 发明授权

US07067314B2 Monoclonal antibody, its immunoreactive fragment and hybridoma 失效
标题翻译：单克隆抗体，其免疫反应性片段和杂交瘤
公开(公告)号：US07067314B2
公开(公告)日：2006-06-27
申请号：US10172986
申请日：2002-06-18
申请人： Takaaki Ohara , Kenji Yamashita , Tetsu Kakutani , Seishi Kyoizumi
发明人： Takaaki Ohara , Kenji Yamashita , Tetsu Kakutani , Seishi Kyoizumi
IPC分类号： C12N5/06 , G01N33/543 , G07K16/00 , C12P21/08
CPC分类号： C07K16/28 , C07K14/47 , G01N33/56966
摘要： It is an object to utilize a novel HSCA-3 antigen occurring in immature hemopoietic stem cells and a monoclonal antibody recognizing said antigen in 1) the immunoassay or flow cytometry involving antibody labeling and 2) the isolation and purification of immature hemopoietic stem cells from human bone marrow cells, umbilical code blood or blood enriched in human hemopoietic stem cells by mobilization with G-CSF.It is directed to a novel HSCA-3 antigen occurring in immature hemopoietic stem cells and to a monoclonal antibody or an immunoreactive fragment thereof which recognizes said antigen.
摘要翻译：本发明的目的是利用在未成熟造血干细胞中发生的新型HSCA-3抗原和识别所述抗原的单克隆抗体，1）免疫测定或流式细胞术涉及抗体标记和2）从人类分离和纯化未成熟造血干细胞骨髓细胞，脐带血或富含人造血干细胞的血液用G-CSF动员。涉及发生在未成熟造血干细胞中的新型HSCA-3抗原以及识别所述抗原的单克隆抗体或其免疫反应性片段。

3. 发明授权

US07445793B2 Support for tissue regeneration and process for producing the same 有权
标题翻译：支持组织再生和生产过程
公开(公告)号：US07445793B2
公开(公告)日：2008-11-04
申请号：US10527075
申请日：2003-09-09
申请人： Hideo Niwa , Takeshi Fukuchi , Ichiro Shimizu , Masao Sato , Akiko Nishi , Kenji Yamashita , Tadashi Kaneko , Hajime Ohgushi , Koji Hattori , Kota Uematsu
发明人： Hideo Niwa , Takeshi Fukuchi , Ichiro Shimizu , Masao Sato , Akiko Nishi , Kenji Yamashita , Tadashi Kaneko , Hajime Ohgushi , Koji Hattori , Kota Uematsu
IPC分类号： A61F2/02 , A61F2/00 , A61F2/28 , A61K9/14 , A61K48/00
CPC分类号： A61L27/3895 , A61L27/3852 , A61L27/56 , A61L2430/06 , C12N5/0068 , C12N5/0655 , C12N2533/40
摘要： The present invention provides a scaffold in which cells can be stably retained and grafted in a uniform distribution state in the culture, preferable proliferation ability and viability can be secured, and particularly in the case of cartilage, fixation treatment such as suture can be carried out in the transplantation into affected parts after the culture, and the mechanical strength is provided sustainable for (weighted) compression at the initial stage of transplantation.The present invention relates to a 3-dimensional porous scaffold for tissue regeneration which comprises a structure composed of vertically long-shaped pores having a pore diameter of not less than 10 μm to not more than 500 μm and pore length of not less than 20 μm to not more than 1 cm being juxtaposedly arranged obtained by a production process comprising rapid freeze-drying as a key technology. Further, the invention relates to the above 3-dimensional porous scaffold in which seeding properties of cells are improved by a pore enlargement treatment of one side face by a separation operation, a salt elution operation of a surface part, or a combination of these operations. It becomes possible to produce a 3-dimensional cell combination having excellent degree of tissue formation and medical treatment effect by seeding a cell or precursor cell derived from a tissue in this scaffold, and culturing them in an artificial environment and/or the living body.
摘要翻译：本发明提供了一种支架，其中细胞可以在培养物中以均匀的分布状态稳定地保持和接枝，可以确保优选的增殖能力和活力，特别是在软骨的情况下，可以进行诸如缝合的定影处理在培养后移植到受影响的部位，并且在移植的初始阶段提供可持续的（加权）压缩的机械强度。本发明涉及用于组织再生的三维多孔支架，其包括由垂直长孔形成的结构，其孔径不小于10μm至不大于500μm，孔长度不小于20μm 通过包括快速冷冻干燥作为关键技术的生产方法并排布置而不超过1cm。此外，本发明涉及上述三维多孔支架，其中通过分离操作，表面部分的盐洗脱操作或这些操作的组合，通过单侧面的孔扩大处理来提高细胞的接种性能。可以通过将来自该支架中的组织的细胞或前体细胞接种，并在人造环境和/或生物体中进行培养，来生产具有优异的组织形成程度和医疗效果的3维细胞组合。

4. 发明申请

US20060127368A1 Support for tissue regeneration and process for producing the same 有权
标题翻译：支持组织再生和生产过程
公开(公告)号：US20060127368A1
公开(公告)日：2006-06-15
申请号：US10527075
申请日：2003-09-09
申请人： Hideo Niwa , Takeshi Fukuchi , Ichiro Shimizu , Masao Sato , Akiko Nishi , Kenji Yamashita , Tadashi Kaneko , Hajime Ohgushi , Koji Hattori , Kota Uematsu
发明人： Hideo Niwa , Takeshi Fukuchi , Ichiro Shimizu , Masao Sato , Akiko Nishi , Kenji Yamashita , Tadashi Kaneko , Hajime Ohgushi , Koji Hattori , Kota Uematsu
IPC分类号： A61K35/12 , C12N5/00
CPC分类号： A61L27/3895 , A61L27/3852 , A61L27/56 , A61L2430/06 , C12N5/0068 , C12N5/0655 , C12N2533/40
摘要： The present invention provides a scaffold in which cells can be stably retained and grafted in a uniform distribution state in the culture, preferable proliferation ability and viability can be secured, and particularly in the case of cartilage, fixation treatment such as suture can be carried out in the transplantation into affected parts after the culture, and the mechanical strength is provided sustainable for (weighted) compression at the initial stage of transplantation. The present invention relates to a 3-dimensional porous scaffold for tissue regeneration which comprises a structure composed of vertically long-shaped pores having a pore diameter of not less than 10 μm to not more than 500 μm and pore length of not less than 20 μm to not more than 1 cm being juxtaposedly arranged obtained by a production process comprising rapid freeze-drying as a key technology. Further, the invention relates to the above 3-dimensional porous scaffold in which seeding properties of cells are improved by a pore enlargement treatment of one side face by a separation operation, a salt elution operation of a surface part, or a combination of these operations. It becomes possible to produce a 3-dimensional cell combination having excellent degree of tissue formation and medical treatment effect by seeding a cell or precursor cell derived from a tissue in this scaffold, and culturing them in an artificial environment and/or the living body.
摘要翻译：本发明提供了一种支架，其中细胞可以在培养物中以均匀的分布状态稳定地保持和接枝，可以确保优选的增殖能力和活力，特别是在软骨的情况下，可以进行诸如缝合的定影处理在培养后移植到受影响的部位，并且在移植的初始阶段提供可持续的（加权）压缩的机械强度。本发明涉及用于组织再生的三维多孔支架，其包括由垂直长孔形成的结构，其孔径不小于10μm至不大于500μm，孔长度不小于20μm 通过包括快速冷冻干燥作为关键技术的生产方法并排布置而不超过1cm。此外，本发明涉及上述三维多孔支架，其中通过分离操作，表面部分的盐洗脱操作或这些操作的组合，通过单侧面的孔扩大处理来提高细胞的接种性能。可以通过将来自该支架中的组织的细胞或前体细胞接种，并在人造环境和/或生物体中进行培养，来生产具有优异的组织形成程度和医疗效果的3维细胞组合。

5. 发明授权

US4988624A Lymphotoxin DNA, lymphotoxin expression vector 失效
标题翻译：淋巴毒素DNA，淋巴毒素表达载体
公开(公告)号：US4988624A
公开(公告)日：1991-01-29
申请号：US882109
申请日：1986-07-03
申请人： Tetsu Kakutani , Kenji Yamashita , yasuhiro Ikenaka , Hajime Kawaharada , Kiyoshi Watanabe
发明人： Tetsu Kakutani , Kenji Yamashita , yasuhiro Ikenaka , Hajime Kawaharada , Kiyoshi Watanabe
IPC分类号： C07K14/525 , C12N15/19 , C12N15/85
CPC分类号： C07K14/525 , C12N15/85
摘要： A chromosomal DNA sequence which codes for human lymphotoxin, a lymphotoxin expression vector which contains a DNA sequence wherein a chromosomal DNA sequence coding for human lymphotoxin and promoter region which functions in animal cell are linked together, lymphotoxin resistant cell line, transformed animal cell culture which is formed by transforming cultured animal cell with a lymphotoxin expression vector which contains a chromosomal DNA sequence coding for human lymphotoxin and, a process for preparing human lymphotoxin, which comprises transforming cultured animal cell with a lymphotoxin expression vector which contains a chromosomal DNA sequence coding for human lymphotoxin, culturing the transformed cell culture to produce human lymphotoxin, and collecting the human lymphotoxin.According to the present invention, LT which is expected for application as the antitumor agent can be produced effectively in a large amount.
摘要翻译：编码人淋巴毒素的染色体DNA序列，含有DNA序列的淋巴毒素表达载体，其中编码人类淋巴毒素的染色体DNA序列和在动物细胞中起作用的启动子区连接在一起，淋巴毒素抗性细胞系，转化的动物细胞培养物是通过用含有编码人淋巴毒素的染色体DNA序列的淋巴毒素表达载体转化培养的动物细胞，以及制备人淋巴毒素的方法形成的，其包括用含有染色体DNA序列编码的淋巴毒素表达载体转化培养的动物细胞，人淋巴毒素，培养转化的细胞培养物以产生人淋巴毒素，并收集人淋巴毒素。根据本发明，可以有效地大量生产预期用作抗肿瘤剂的LT。

6. 发明申请

US20070031894A1 Novel nerve stem cell marker 审中-公开
标题翻译：新型神经干细胞标记
公开(公告)号：US20070031894A1
公开(公告)日：2007-02-08
申请号：US10571268
申请日：2004-09-10
申请人： Tomoko Syofuda , Yonehiro Kanemura , Jun Miyake , Hideo Niwa , Kenji Yamashita
发明人： Tomoko Syofuda , Yonehiro Kanemura , Jun Miyake , Hideo Niwa , Kenji Yamashita
IPC分类号： G01N33/567 , C07K14/475 , C07K16/22 , C07H21/04
CPC分类号： G01N33/5058 , C12Q1/6876 , G01N33/5008 , G01N33/5023 , G01N33/5073 , G01N2500/00
摘要： It is an object of the present invention to provide a method for detecting nerve stem cells/progenitor cells among a tissue or a mass of cells as constituted of a plurality of cell species, and a method for screening for a drug functioning as a nerve differentiation factor or the like by using the above-mentioned detection method. It was found in this invention that the expression of the NC1 gene was high in undifferentiated nerve stem cells and said expression decreased with an advance of differentiation. Furthermore in this invention, by using the NC1 gene, the product thereof (NC1 protein) and the base sequences or the amino acid sequences thereof, antibodies or the like, it became possible to detect nerve stem cells/progenitor cells by way of various methods, so that a novel method for screening for a drug functioning as a nerve differentiation factor or the like was provided.
摘要翻译：本发明的目的是提供一种由多种细胞种组成的组织或大量细胞中的神经干细胞/祖细胞的检测方法，以及筛选作为神经分化的药物的方法因子等通过使用上述检测方法。在本发明中发现NC1基因的表达在未分化神经干细胞中高，并且所述表达随着分化的进展而降低。此外，在本发明中，通过使用NC1基因，其产物（NC1蛋白）及其碱基序列或其氨基酸序列，抗体等，可以通过各种方法检测神经干细胞/祖细胞，因此提供了筛选用作神经分化因子等的药物的新方法。

7. 发明授权

US06764688B2 Method for inducing immunosuppressive cells and a culture device to be used therefor 失效
标题翻译：诱导免疫抑制细胞的方法和用于其的培养装置
公开(公告)号：US06764688B2
公开(公告)日：2004-07-20
申请号：US09254170
申请日：1999-03-02
申请人： Kenji Yamashita , Takeshi Fukuchi , Tomoko Nishino
发明人： Kenji Yamashita , Takeshi Fukuchi , Tomoko Nishino
IPC分类号： A61K4500
CPC分类号： C12N5/0636 , A61K2035/122 , C12M25/00 , C12N2501/23 , C12N2501/515 , C12N2501/599
摘要： The present invention provides a method for inducing immunosuppressive cells by cultivating human cells with the use of a culture device having an affinity for protein and the culture device to be used therefor. According to the present invention, immunosuppressive cells can be efficiently induced by using a solid culture and can provide an efficient therapeutic system for diseases caused by hypersensitivity of the immune system with less side effect.
摘要翻译：本发明提供通过使用对蛋白质具有亲和力的培养装置和用于其的培养装置培养人细胞来诱导免疫抑制细胞的方法。根据本发明，可以通过使用固体培养物有效地诱导免疫抑制细胞，并且可以为免疫系统的超敏反应引起的疾病提供有效的治疗系统，而副作用较小。

8. 发明申请

US20060057645A1 Novel genes 审中-公开
标题翻译：新基因
公开(公告)号：US20060057645A1
公开(公告)日：2006-03-16
申请号：US10507876
申请日：2003-03-06
申请人： Hideo Niwa , Kenji Yamashita
发明人： Hideo Niwa , Kenji Yamashita
IPC分类号： G01N33/567 , C07H21/04 , C12N5/08 , C07K16/26
CPC分类号： C12Q1/6883 , C07K14/47
摘要： The object of the present invention is to detect and select insulin-producing cells capable of proliferating, and further to differentiate/proliferate insulin-producing cells and precursory thereof, or cells related thereto. The present invention was made by finding three species of novel genes by detecting genes specifically expressed in pancreases of PHHI patients which are regarded as a model of spontaneous proliferation of pancreatic β cells, and searching for base sequence data bases. According to the present invention, proliferating insulin-producing cells can be detected using these genes, gene products or gene sequences by, for example, Northern analysis or RT-PCR. Furthermore, these genes can be differentiated into insulin-producing cells by introducing these genes into appropriate cells in genetic engineering manner.
摘要翻译：本发明的目的是检测和选择能够增殖的胰岛素生成细胞，并且进一步分化/增殖胰岛素产生细胞及其前体，或与其相关的细胞。本发明是通过检测被认为是胰腺β细胞自发增殖的模型，以及寻找碱基序列数据库的PHHI患者胰腺特异性表达的基因来发现三种新型基因。根据本发明，通过例如Northern分析或RT-PCR，可以使用这些基因，基因产物或基因序列检测增殖性胰岛素生成细胞。此外，这些基因可以通过将这些基因以遗传工程方式引入合适的细胞中而分化成胰岛素生成细胞。

9. 发明授权

US08875891B2 Press-through package and method of removing medication from same 有权
标题翻译：直通式包装及从其中去除药物的方法
公开(公告)号：US08875891B2
公开(公告)日：2014-11-04
申请号：US13108685
申请日：2011-05-16
申请人： Kenji Yamashita , Akiyoshi Yasusato , Tetsuya Yamashita , Kenji Hamada , Goro Kuratani , Masakazu Sugino , Yuko Hanazono , Yoshihiko Matsuoka
发明人： Kenji Yamashita , Akiyoshi Yasusato , Tetsuya Yamashita , Kenji Hamada , Goro Kuratani , Masakazu Sugino , Yuko Hanazono , Yoshihiko Matsuoka
IPC分类号： B65D83/04 , A61J1/03 , B65D75/32
CPC分类号： B65D75/327 , A61J1/035 , B65D83/0445 , Y10T29/49826
摘要： A press-through package for articles such as medicines which can reliably prevent a recipient from accidentally swallowing the whole package without opening it, including solid medicines M stored in a plurality of pockets of a base sheet one by one and the pockets sealed by a cover film having attached to the rear surface of the base sheet, wherein the entire package of the press-through package is made in a cylindrical form which cannot allow a recipient from accidentally swallowing and from which the medicines can be taken out easily.
摘要翻译：一种用于能够可靠地防止接收者意外吞咽整个包装而不打开它的物品的压穿包装，包括一个接一个地存储在基片的多个口袋中的固体药物M和由盖子封闭的口袋薄膜附着在基片的后表面上，其中压入包装的整个包装制成圆柱形，不能容许接收者意外吞咽，并且药物可以容易地从中取出。

10. 发明授权

US08073091B2 Signal processing method and circuit to convert analog signal to digital signal 有权
标题翻译：信号处理方法和电路将模拟信号转换为数字信号
公开(公告)号：US08073091B2
公开(公告)日：2011-12-06
申请号：US12320324
申请日：2009-01-23
申请人： Kenji Yamashita
发明人： Kenji Yamashita
IPC分类号： H04L7/04
CPC分类号： H04N5/06 , G09G5/008 , H04N5/126
摘要： A phase determination unit in a signal processing circuit generates sampling clocks with different phases in a clock generator and sequentially provides them to an analog-to-digital convertor. Then, the phase determination unit obtains differences between each adjacent two signal levels in each sampled digital signal by use of the sampling clocks, and monitors a polarity change in the differences, extracts a more inappropriate phase for use in sampling from phases of the sampling clocks on the basis of the absolute values of the differences where the polarity change is detected, and determines an antiphase of the extracted phase as a phase of the sampling clock to be provided to the analog-to-digital convertor.
摘要翻译：信号处理电路中的相位确定单元在时钟发生器中产生具有不同相位的采样时钟，并将它们顺序地提供给模数转换器。然后，相位确定单元通过使用采样时钟获得每个采样数字信号中每个相邻两个信号电平之间的差异，并且监视差异中的极性变化，从采样时钟的相位提取用于采样的更不合适的相位基于检测到极性变化的差异的绝对值，并将所提取的相位的反相确定为要提供给模数转换器的采样时钟的相位。

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