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1. 发明申请

US20110104685A1 METHOD FOR TESTING MICROORGANISM OR THE LIKE AND TESTING APPARATUS THEREFOR 有权
标题翻译：用于测试微生物或其类似物和测试装置的方法
公开(公告)号：US20110104685A1
公开(公告)日：2011-05-05
申请号：US12914055
申请日：2010-10-28
申请人： Kei Takenaka , Hideki Nakamoto , Kazuo Takei , Masahiro Kurihara , Yuusuke Watanabe , Hisao Saito , Yasuhiko Sasaki
发明人： Kei Takenaka , Hideki Nakamoto , Kazuo Takei , Masahiro Kurihara , Yuusuke Watanabe , Hisao Saito , Yasuhiko Sasaki
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： G01N21/6428 , G01J3/36 , G01J3/4406 , G01N21/645 , G01N2021/6421 , G01N2021/6441
摘要： A means for accurately counting desired cells or microorganisms (viable bacteria) in a sample fluid in which contaminants are included is provided. One or plural types of membrane-permeable fluorochromes whose fluorescence amount is amplified by binding to a nucleic acid and glycerin are added to a sample fluid containing cells or microorganisms to be counted and allowed to stand for a certain time. Glycerin is added before or after or simultaneously with the mixing of the sample fluid and the fluorochrome(s). The cells or microorganisms to be counted are counted by staining the cells or microorganisms to be counted, followed by irradiating with light having a specific wavelength to detect the fluorescence emitted from the cells or microorganisms.
摘要翻译：提供了在包含污染物的样品流体中精确计数期望的细胞或微生物（活细菌）的方法。将其荧光量通过与核酸和甘油结合而扩增的一种或多种透膜型荧光染料加入含有要计数并允许静置一定时间的细胞或微生物的样品液中。在样品流体和荧光染料的混合之前或之后或同时加入甘油。通过染色要计数的细胞或微生物计数细胞或微生物，然后用特定波长的光照射以检测从细胞或微生物发出的荧光。

2. 发明授权

US08889349B2 Method for testing microorganism or the like and testing apparatus therefor 有权
标题翻译：微生物等的检测方法及其检测装置
公开(公告)号：US08889349B2
公开(公告)日：2014-11-18
申请号：US12914055
申请日：2010-10-28
申请人： Kei Takenaka , Hideki Nakamoto , Kazuo Takei , Masahiro Kurihara , Yuusuke Watanabe , Hisao Saito , Yasuhiko Sasaki
发明人： Kei Takenaka , Hideki Nakamoto , Kazuo Takei , Masahiro Kurihara , Yuusuke Watanabe , Hisao Saito , Yasuhiko Sasaki
IPC分类号： C12Q1/68 , C12Q1/02 , C12Q1/04 , C12Q1/06 , G01N1/30 , G01N21/64 , G01J3/36 , G01J3/44
CPC分类号： G01N21/6428 , G01J3/36 , G01J3/4406 , G01N21/645 , G01N2021/6421 , G01N2021/6441
摘要： A method for accurately counting desired cells or microorganisms (viable bacteria) in a sample fluid in which contaminants are included is provided. One or plural types of membrane-permeable fluorochromes whose fluorescence amount is amplified by binding to a nucleic acid and glycerin are added to a sample fluid containing cells or microorganisms to be counted and allowed to stand for a certain time. Glycerin is added before or after or simultaneously with the mixing of the sample fluid and the fluorochrome(s). The cells or microorganisms to be counted are counted by staining the cells or microorganisms to be counted, followed by irradiating with light having a specific wavelength to detect the fluorescence emitted from the cells or microorganisms.
摘要翻译：提供了在包含污染物的样品流体中精确计数期望的细胞或微生物（活细菌）的方法。将其荧光量通过与核酸和甘油结合而扩增的一种或多种透膜型荧光染料加入含有要计数并允许静置一定时间的细胞或微生物的样品液中。在样品流体和荧光染料的混合之前或之后或同时加入甘油。通过染色要计数的细胞或微生物计数细胞或微生物，然后用特定波长的光照射以检测从细胞或微生物发出的荧光。

3. 发明授权

US08404480B2 Microorganism testing device and microorganism testing chip 有权
标题翻译：微生物检测装置和微生物检测芯片
公开(公告)号：US08404480B2
公开(公告)日：2013-03-26
申请号：US12575489
申请日：2009-10-08
申请人： Kei Takenaka , Yasuhiko Sasaki , Hideki Nakamoto , Kazuo Takei , Masahiro Kurihara , Yuusuke Watanabe , Hisao Saito
发明人： Kei Takenaka , Yasuhiko Sasaki , Hideki Nakamoto , Kazuo Takei , Masahiro Kurihara , Yuusuke Watanabe , Hisao Saito
IPC分类号： C12M1/34 , G01N21/03
CPC分类号： G01N15/14 , B01L3/502707 , B01L2300/0867 , B01L2300/087 , B01L2400/0487 , G01N21/6428 , G01N2015/1452 , G01N2021/6441 , Y10T29/49826
摘要： Provided are a microorganism testing chip capable of suppressing self-fluorescence and enhancing mass productivity, and a microorganism testing device using the same. The microorganism testing chip includes a main body and a fungus body detection unit mounted on the main body. The main body has a detection window frame portion which is a through-hole or a pass-through groove. The fungus body detection unit is disposed to cover the detection window frame portion and has a fungus body detection flow path connected to flow paths provided in the main body. The fungus body detection unit includes a cover member and a flow path member, and is formed by attaching these two members to each other. The flow path member has a groove. By attaching the two members to each other, the groove of the flow path member serves as the fungus body detection flow path.
摘要翻译：提供能够抑制自发荧光并提高批量生产率的微生物测试芯片，以及使用其的微生物测试装置。微生物检测芯片包括安装在主体上的主体和真菌体检测单元。主体具有作为通孔或贯通槽的检测窗框架部。真菌体检测单元被设置为覆盖检测窗框部，并且具有连接到设置在主体中的流路的真菌体检测流路。真菌体检测单元包括盖构件和流路构件，并且通过将这两个构件彼此附接而形成。流路构件具有凹槽。通过将两个构件相互连接，流路构件的槽用作真菌体检测流路。

4. 发明申请

US20100273208A1 MICROORGANISM TESTING APPARATUS 审中-公开
标题翻译：微生物测试仪器
公开(公告)号：US20100273208A1
公开(公告)日：2010-10-28
申请号：US12766948
申请日：2010-04-26
申请人： Kei Takenaka , Hideki Nakamoto , Kazuo Takei , Masahiro Kurihara , Yuusuke Watanabe , Hisao Saito
发明人： Kei Takenaka , Hideki Nakamoto , Kazuo Takei , Masahiro Kurihara , Yuusuke Watanabe , Hisao Saito
IPC分类号： C12Q1/04 , C12M1/34
CPC分类号： C12M41/36 , G01N15/1484 , G01N21/6428 , G01N2015/1477
摘要： To eliminate the necessity of a dedicated optical system and the flowing of fluorescent microparticles for aligning excitation light with a flat plate-shaped flow cell which internally includes a flow path, a microorganism testing apparatus includes: a first detector that detects fluorescence emitted from microorganisms flowing through a detection flow path when a microorganism detection unit included in a microorganism testing chip is irradiated with excitation light, and converts the fluorescence to an electrical signal; and a second detector that detects scattered light similarly emitted from the microorganisms flowing through the detection flow path, and converts the scattered light to an electrical signal. The alignment of the detection flow path is performed in the direction of the optical axis of the excitation light by controlling and moving a stage having the microorganism testing chip mounted thereon based on the intensity of fluorescence detected by the first detector.
摘要翻译：为了消除专用光学系统的需要和用于使激发光与用于内部包括流路的平板状流动池对准的荧光微粒的流动，微生物测试装置包括：第一检测器，其检测从微生物流出的荧光当微生物检测芯片中包含的微生物检测单元被激发光照射时，通过检测流路，并将荧光转换成电信号; 以及第二检测器，其检测类似地从流过检测流路的微生物发射的散射光，并将散射光转换成电信号。基于由第一检测器检测到的荧光强度，通过控制和移动安装有微生物测试芯片的台，来在激发光的光轴的方向上进行检测流路的对准。

5. 发明授权

US06224826B1 Sterilizing method and apparatus 失效
标题翻译：灭菌方法及装置
公开(公告)号：US06224826B1
公开(公告)日：2001-05-01
申请号：US09270846
申请日：1999-03-18
申请人： Kageyoshi Katakura , Masahiro Kurihara , Kazuo Takei , Shinichiro Umemura , Kenichi Kawabata , Hiroshi Masuzawa
发明人： Kageyoshi Katakura , Masahiro Kurihara , Kazuo Takei , Shinichiro Umemura , Kenichi Kawabata , Hiroshi Masuzawa
IPC分类号： A61L200
CPC分类号： A61L2/025 , C02F1/36 , C02F1/48 , C02F2303/04 , Y10S261/48
摘要： A method and apparatus for sterilizing water containing pathogen protozoa having shells in a simple manner without negative influencing the environment is disclosed. Ultrasonic waves are applied to drinking water to form fine cavitation bubbles which are used to destroy the shells of pathogenic protozoa. The residual chlorine in the drinking water along with an oxidizing action and effect of the ultrasonic wave, permit not only sterilization but also deodorization and decolorization.
摘要翻译：公开了以简单的方式对含有病原体原生动物的水灭菌的方法和装置，而不会对环境产生不利影响。超声波应用于饮用水，形成细小的气泡，用于破坏致病原生动物的壳。饮用水中的残留氯以及超声波的氧化作用和作用，不但能消毒灭菌，而且除臭脱色。

6. 发明申请

US20070280861A1 Water treatment method and water treatment device 审中-公开
标题翻译：水处理方法和水处理装置
公开(公告)号：US20070280861A1
公开(公告)日：2007-12-06
申请号：US11236365
申请日：2005-09-26
申请人： Masahiro Kurihara , Kazuo Takei , Kunio Hashiba , Kageyoshi Katakura
发明人： Masahiro Kurihara , Kazuo Takei , Kunio Hashiba , Kageyoshi Katakura
IPC分类号： C02F1/36 , A61L2/08
CPC分类号： C02F1/36 , B06B1/0253 , B06B2201/70 , C02F1/008 , C02F1/76 , C02F2103/42
摘要： Embodiments of the invention provide a water treatment method and water treatment device capable of automatically tracking the frequency of ultrasonic waves and automatically controlling their amplitude such that optimal cavitation is generated. In one embodiment, a water treatment method of sterilizing water containing microbes comprises supplying ultrasonic vibration to the water to be treated by driving and controlling a transducer of a sterilization cell using a signal; detecting an amplitude of the transducer and a frequency of the signal applied to the transducer; and controlling the amplitude and a vibration frequency of the transducer to target values in accordance with the detected transducer amplitude and the detected signal frequency.
摘要翻译：本发明的实施例提供一种水处理方法和水处理装置，其能够自动跟踪超声波的频率并自动控制其振幅，从而产生最佳的空化。在一个实施方案中，对包含微生物的水进行灭菌的水处理方法包括通过使用信号驱动和控制灭菌室的换能器来向待处理的水提供超声波振动; 检测所述换能器的振幅和施加到所述换能器的信号的频率; 以及根据检测到的换能器振幅和检测到的信号频率来将换能器的振幅和振动频率控制为目标值。

7. 发明授权

US07018546B2 Water treatment method and water treatment device 失效
标题翻译：水处理方法和水处理装置
公开(公告)号：US07018546B2
公开(公告)日：2006-03-28
申请号：US10754319
申请日：2004-01-08
申请人： Masahiro Kurihara , Kazuo Takei , Kunio Hashiba , Kageyoshi Katakura
发明人： Masahiro Kurihara , Kazuo Takei , Kunio Hashiba , Kageyoshi Katakura
IPC分类号： C02F1/36
CPC分类号： C02F1/36 , B06B1/0253 , B06B2201/70 , C02F1/008 , C02F1/76 , C02F2103/42
摘要： Embodiments of the invention provide a water treatment method and water treatment device capable of automatically tracking the frequency of ultrasonic waves and automatically controlling their amplitude such that optimal cavitation is generated. In one embodiment, a water treatment method of sterilizing water containing microbes comprises supplying ultrasonic vibration to the water to be treated by driving and controlling a transducer of a sterilization cell using a signal; detecting an amplitude of the transducer and a frequency of the signal applied to the transducer; and controlling the amplitude and a vibration frequency of the transducer to target values in accordance with the detected transducer amplitude and the detected signal frequency.
摘要翻译：本发明的实施例提供一种水处理方法和水处理装置，其能够自动跟踪超声波的频率并自动控制其振幅，从而产生最佳的空化。在一个实施方案中，对包含微生物的水进行灭菌的水处理方法包括通过使用信号驱动和控制灭菌室的换能器来向待处理的水提供超声波振动; 检测所述换能器的振幅和施加到所述换能器的信号的频率; 以及根据检测到的换能器振幅和检测到的信号频率来将换能器的振幅和振动频率控制为目标值。

8. 发明申请

US20070249039A1 MINUTE CREATURE DETECTION SYSTEM AND ANALYTIC CHIP 审中-公开
标题翻译：分钟检测系统和分析芯片
公开(公告)号：US20070249039A1
公开(公告)日：2007-10-25
申请号：US11627402
申请日：2007-01-26
申请人： Mitsuhiro Matsuzawa , Hisao Inami , Yasuhiko Sasaki , Masahiro Kurihara
发明人： Mitsuhiro Matsuzawa , Hisao Inami , Yasuhiko Sasaki , Masahiro Kurihara
IPC分类号： C12M1/34
CPC分类号： B01L3/502746 , B01L3/5027 , B01L7/52 , B01L9/527 , B01L2200/141 , B01L2300/0654 , B01L2300/0681 , B01L2300/069 , B01L2300/0816 , B01L2400/0487 , B01L2400/0688 , B01L2400/084
摘要： It is an object of the present invention to shorten the operation time from sampling through to genetic test, and enable the detection of a minute creature with good accuracy. Provided is minute creature detection system in which treatments from gene extraction to detection of are conducted on an analytic chip, in which the analytic chip 300 contains a sample reservoir 315, a gene extraction area 320 filled with a gene binding carrier, a waste solution chamber 330 filled with absorbent, a washing solution storage chamber 340 which stores washing solution, an eluenting solution storage chamber 370 which stores a gene eluenting solution, a gene-amplification reagent storage chamber 380 which stores a gene-amplification reagent, and a reaction chamber 395 which amplifies and detects the gene, each of which is formed by a channel, and a weir having a channel in at least either the sample reservoir 315 or the reagent storage chamber 380 which contracts and expands.
摘要翻译：本发明的目的是缩短从采样到遗传检测的操作时间，并且能够精确地检测微小的生物。提供了一种分析生物检测系统，其中分析芯片300在分析芯片上进行从基因提取到检测的处理，其中分析芯片300包含样品储存器315，填充有基因结合载体的基因提取区域320，废液溶液室 330，填充有吸收剂，存储洗涤溶液的洗涤溶液储存室340，存储基因洗脱溶液的洗脱溶液储存室370，存储基因扩增试剂的基因扩增试剂储存室380和反应室395 其放大并检测每个由通道形成的基因，以及在至少收集和扩展的样品储存器315或试剂储存室380中的通道的堰。

9. 发明授权

US6020874A Flat panel display device 失效
标题翻译：平板显示设备
公开(公告)号：US6020874A
公开(公告)日：2000-02-01
申请号：US936739
申请日：1997-09-25
申请人： Masahiro Kurihara
发明人： Masahiro Kurihara
IPC分类号： G02F1/133 , G09G3/20 , G09G3/36 , G09G5/00
CPC分类号： G09G3/3611 , G09G3/3685 , G09G2310/0232 , G09G2310/027 , G09G3/20 , G09G3/3648
摘要： A flat panel display device includes a display panel including a plurality of pixels arranged in matrix of rows and columns, a data latch circuit for latching various types of color data to be displayed on a display area having a predetermined resolution on the display panel, a specific color data generation circuit for generating specific color data to be displayed on a non-display area on the display panel, and a timing control circuit for controlling timing such that the specific color data generated from the specific color data generation circuit is displayed on the non-display area simultaneously with an operation of displaying the various types of color data latched by the data latch circuit on the display area.
摘要翻译：平板显示装置包括：显示面板，包括以行和列为矩阵排列的多个像素;数据锁存电路，用于锁存要在显示面板上具有预定分辨率的显示区域上显示的各种颜色数据; 特定颜色数据生成电路，用于生成要显示在显示面板上的非显示区域上的特定颜色数据;以及定时控制电路，用于控制定时，使得从特定颜色数据产生电路产生的特定颜色数据显示在非显示区域同时显示由显示区域上的数据锁存电路锁存的各种颜色数据的操作。

10. 发明申请

US20070281350A1 Bacteria trapping apparatus 审中-公开
标题翻译：细菌捕获装置
公开(公告)号：US20070281350A1
公开(公告)日：2007-12-06
申请号：US11236788
申请日：2005-09-28
申请人： Hisao Inami , Masahiro Kurihara
发明人： Hisao Inami , Masahiro Kurihara
IPC分类号： C12M1/34
CPC分类号： G01N1/2273 , G01N1/2205 , G01N2001/2223
摘要： A bacteria trapping apparatus comprises a nozzle for introducing air, a trapping chamber disposed on an exit side of the nozzle, and a trapping material disposed inside the trapping chamber for trapping bacteria. The air introduced into the trapping chamber is discharged by a discharge unit towards the side opposite to the nozzle. The trapping material, a trapping container, and a holding member together constitute a trapping unit, which preferably comprises a bacteria trapping chip that can be mounted on analysis equipment.
摘要翻译：细菌捕获装置包括用于引入空气的喷嘴，设置在喷嘴的出口侧的捕集室，以及设置在捕集室内部用于捕获细菌的捕集材料。引入捕集室的空气通过排出单元朝与喷嘴相对的一侧排出。捕集材料，捕集容器和保持构件一起构成捕获单元，其优选包括可以安装在分析设备上的细菌捕获芯片。

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