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1. 发明申请

US20060216795A1 Sample preparation method, analysis system and preparation device 审中-公开
标题翻译：样品制备方法，分析系统及制备装置
公开(公告)号：US20060216795A1
公开(公告)日：2006-09-28
申请号：US11356114
申请日：2006-02-17
申请人： Katsuhiro Kanda , Yukie Sasakura , Tsuyoshi Ogino , Makoto Nogami
发明人： Katsuhiro Kanda , Yukie Sasakura , Tsuyoshi Ogino , Makoto Nogami
IPC分类号： C12P19/24
CPC分类号： C12N9/80 , C12N9/6427 , C12N11/14 , C12N11/18 , C12Y305/01052
摘要： The object of the present invention is to promote the efficiency and simplify a sample pretreatment step for analysis of molecules contained in the sample. In the present invention, a plurality of various enzymes necessary for pretreatment of sample for analysis of various molecules contained in the sample are immobilized on a solid phase separately for each kind of enzyme, and the enzyme reactions are carried out simultaneously. That is, a sample is reacted with plural kinds of enzymes under the same conditions, and contamination caused by the enzyme reactions is inhibited and the sample is subjected to a given pretreatment.
摘要翻译：本发明的目的是提高效率并简化用于分析样品中所含分子的样品预处理步骤。在本发明中，将用于分析样品中所含的各种分子的样品预处理所需的多种各种酶分别固定在固相上用于各种酶，并且酶反应同时进行。也就是说，样品在相同条件下与多种酶反应，并且抑制由酶反应引起的污染并且对样品进行给定的预处理。

2. 发明申请

US20070037213A1 Method of analyzing protein and kit for analyzing protein 审中-公开
标题翻译：分析蛋白质和试剂盒分析蛋白质的方法
公开(公告)号：US20070037213A1
公开(公告)日：2007-02-15
申请号：US11498279
申请日：2006-08-03
申请人： Yukie Sasakura , Katsuhiro Kanda
发明人： Yukie Sasakura , Katsuhiro Kanda
IPC分类号： G01N33/53
CPC分类号： G01N33/68 , G01N33/6812 , G01N33/6842 , G01N33/6845
摘要： The expression and the phosphorylation level of a target protein are determined without performing complicated operations involving risk; the expression and the phosphorylation level of the target protein are simultaneously determined, by separating and detecting the specific protein from other proteins different from the specific protein, and by specifically detecting a phosphorylated residue of the identified target protein; and finally a ratio of the target protein phosphorylated is calculated to obtain the phosphorylation ratio of the protein.
摘要翻译：确定目标蛋白的表达和磷酸化水平，而不进行涉及风险的复杂操作; 通过从不同于特定蛋白质的其他蛋白质中分离和检测特异性蛋白质，并通过特异性检测鉴定的靶蛋白质的磷酸化残基，同时确定靶蛋白的表达和磷酸化水平; 最后，计算磷酸化蛋白质的比例，得到蛋白质的磷酸化比例。

3. 发明申请

US20050255526A1 Anti-tag antibody chip for protein interaction analysis 审中-公开
标题翻译：抗标签抗体芯片进行蛋白质相互作用分析
公开(公告)号：US20050255526A1
公开(公告)日：2005-11-17
申请号：US11121118
申请日：2005-05-04
申请人： Katsuhiro Kanda , Yukie Sasakura
发明人： Katsuhiro Kanda , Yukie Sasakura
IPC分类号： G01N33/53 , C07K1/22 , C12N15/09 , G01N21/27 , G01N21/78 , G01N33/00 , G01N33/543 , G01N33/58 , G01N37/00 , C12M1/34
CPC分类号： G01N33/54373
摘要： It is an object of the present invention to conduct function analysis of proteins arranged with high density on a solid phase in a high-throughput manner, regardless of protein varieties. The present invention is characterized in that it immobilizes target proteins on a substrate while maintaining the structures and the functions thereof, and that it carries out interaction analysis of such proteins. For example, target proteins are prepared in a state where one end of each protein is fused to a peptide as a tag or to a polypeptide by genetic engineering methods. Then, the target proteins are bonded to the substrate via a layer formed thereon, comprising anti-tag antibodies. The target proteins are arrayed while they are maintained in a liberated state on a solid phase, and the interaction analysis is carried out. According to the present invention, the interaction analysis of target proteins can be carried out without affecting the unstable structures and the functions thereof. For example, the present invention can be applied as a platform for interaction analysis, screening, quantitation, expression profiling, or the like, regarding proteins.
摘要翻译：本发明的一个目的是以高通量方式对固相高密度蛋白质进行功能分析，而与蛋白质品种无关。本发明的特征在于，其将靶蛋白固定在底物上，同时保持其结构及其功能，并且进行这些蛋白质的相互作用分析。例如，通过基因工程方法，将各蛋白质的一端作为标签或多肽融合的状态制备靶蛋白质。然后，靶蛋白经由其上形成的层与底物结合，包含抗标签抗体。将靶蛋白排列在固相的释放状态下，进行相互作用分析。根据本发明，靶蛋白的相互作用分析可以在不影响不稳定结构及其功能的情况下进行。例如，本发明可以用作蛋白质的相互作用分析，筛选，定量，表达谱等的平台。

4. 发明授权

US09207152B2 Pretreatment apparatus and mass analyzing apparatus equipped with the same 有权
标题翻译：预处理装置和配备有其的质量分析装置
公开(公告)号：US09207152B2
公开(公告)日：2015-12-08
申请号：US13377154
申请日：2010-06-24
申请人： Makoto Nogami , Katsuhiro Kanda , Izumi Waki
发明人： Makoto Nogami , Katsuhiro Kanda , Izumi Waki
IPC分类号： G01N33/48 , G01N1/40 , G01N35/02 , G01N30/00 , H01J49/00
CPC分类号： G01N1/4055 , G01N35/025 , G01N2001/4061 , G01N2030/009 , H01J49/00
摘要： A biological sample preprocessing apparatus includes solid-phase extraction cartridges which purify/concentrate specific constituents of a biological sample, cartridge holders which hold one of the solid-phase extraction cartridges, a cartridge transport that carries the cartridge holders along a continuous track surface, a sample probe that discharges the biological sample into the solid-phase extraction cartridge held in the cartridge holders, a water-based solvent probe that discharges a water-based solvent into the solid-phase extraction cartridge held in the cartridge holder, an organic solvent probe that discharges an organic solvent into the solid-phase extraction cartridge held in the cartridge holder, a pressure loader that applies an air pressure load to the solid-phase extraction cartridge held in the cartridge holder, and a control unit that controls operation of the cartridge transport, the sample probe, the solvent probes, and the pressure loader, wherein sample addition and solvent addition alternate with pressure loading.
摘要翻译：生物样品预处理装置包括净化/浓缩生物样品的特定组分的固相萃取盒，固定一个固相萃取筒的盒保持器，沿连续轨迹表面承载盒保持器的盒输送器将生物样品排出到保持在盒保持器中的固相提取盒中的样品探针，将水性溶剂排放到保持在盒保持器中的固相萃取盒中的水性溶剂探针，有机溶剂探针将有机溶剂排放到保持在盒保持器中的固相提取盒中，对保持在盒保持器中的固相提取盒施加空气压力负荷的压力装载器和控制盒的操作的控制单元运输，样品探针，溶剂探针和压力装载机，其中样品添加溶剂添加与压力负荷交替。

5. 发明申请

US20120079875A1 PRETREATMENT APPARATUS AND MASS ANALYZING APPARATUS EQUIPPED WITH THE SAME 有权
标题翻译：预装设备和配有同类设备的大规模分析设备
公开(公告)号：US20120079875A1
公开(公告)日：2012-04-05
申请号：US13377154
申请日：2010-06-24
申请人： Makoto Nogami , Katsuhiro Kanda , Izumi Waki
发明人： Makoto Nogami , Katsuhiro Kanda , Izumi Waki
IPC分类号： G01N1/10 , G01N1/28
CPC分类号： G01N1/4055 , G01N35/025 , G01N2001/4061 , G01N2030/009 , H01J49/00
摘要： A biological sample preprocessing apparatus includes solid-phase extraction cartridges which purify/concentrate specific constituents of a biological sample, cartridge holders which hold one of the solid-phase extraction cartridges, a cartridge transport that carries the cartridge holders along a continuous track surface, a sample probe that discharges the biological sample into the solid-phase extraction cartridge held in the cartridge holders, a water-based solvent probe that discharges a water-based solvent into the solid-phase extraction cartridge held in the cartridge holder, an organic solvent probe that discharges an organic solvent into the solid-phase extraction cartridge held in the cartridge holder, a pressure loader that applies an air pressure load to the solid-phase extraction cartridge held in the cartridge holder, and a control unit that controls operation of the cartridge transport, the sample probe, the solvent probes, and the pressure loader, wherein sample addition and solvent addition alternate with pressure loading.
摘要翻译：生物样品预处理装置包括净化/浓缩生物样品的特定组分的固相萃取盒，固定一个固相萃取筒的盒保持器，沿连续轨迹表面承载盒保持器的盒输送器将生物样品排出到保持在盒保持器中的固相提取盒中的样品探针，将水性溶剂排放到保持在盒保持器中的固相萃取盒中的水性溶剂探针，有机溶剂探针将有机溶剂排放到保持在盒保持器中的固相提取盒中，对保持在盒保持器中的固相提取盒施加空气压力负荷的压力装载器和控制盒的操作的控制单元运输，样品探针，溶剂探针和压力装载机，其中样品添加溶剂添加与压力负荷交替。

6. 发明授权

US08730459B2 Examination device and examination method 有权
标题翻译：考试设备和考试方法
公开(公告)号：US08730459B2
公开(公告)日：2014-05-20
申请号：US13501941
申请日：2010-10-15
申请人： Makoto Nogami , Katsuhiro Kanda , Shinya Ito , Izumi Waki
发明人： Makoto Nogami , Katsuhiro Kanda , Shinya Ito , Izumi Waki
IPC分类号： G01N33/48
CPC分类号： G01N35/025 , G01N1/405 , G01N1/4077 , G01N2035/00485 , G01N2035/0427 , G01N2035/0441
摘要： Provided herein are an examination device and an examination method that can efficiently perform examination even when both whole blood and serum/blood plasma specimens are to be examined. A solid-phase extraction cartridge or a filter is placed on a continuous track of a cartridge table. A cup table is disposed below the cartridge table. A cup is placed on a continuous track of the cup table to receive a sample purified by the filter. When viewed from above, the continuous track of the cartridge table and the continuous track of the cup table cross each other at a position “m”, and each of the tracks crosses a position at which a sample probe operates. A pretreatment for serum/blood plasma is completed in one rotation of the cartridge table. A pretreatment for whole blood is completed in two rotations of the cartridge table.
摘要翻译：这里提供即使在检查全血和血清/血浆样本时也能够有效地进行检查的检查装置和检查方法。固相提取盒或过滤器放置在墨盒台的连续轨道上。一个杯子桌子放置在墨盒桌子下方。将杯子放置在杯台的连续轨道上以接收由过滤器净化的样品。当从上方观察时，墨盒台的连续轨道和杯台的连续轨道在位置“m”处彼此交叉，并且每个轨道与样品探针操作的位置相交。血清/血浆的预处理在药筒表的一个旋转中完成。全血的预处理在墨盒桌子的两个旋转中完成。

7. 发明申请

US20110287446A1 IMMUNOANALYTICAL METHOD AND SYSTEM USING MASS SPECTROMETRY TECHNOLOGY 有权
标题翻译：使用大规模光谱技术的免疫学方法和系统
公开(公告)号：US20110287446A1
公开(公告)日：2011-11-24
申请号：US13146952
申请日：2010-02-09
申请人： Katsuhiro Kanda , Makoto Nogami , Izumi Waki
发明人： Katsuhiro Kanda , Makoto Nogami , Izumi Waki
IPC分类号： G01N33/53 , C12M1/34 , G01N33/566 , G01N30/00
CPC分类号： G01N33/6848
摘要： An immunoanalytical system in which, after a sample such as a patient's serum is subjected to a pretreatment by an immunological pretreatment device, the sample is subjected to light detection by an immunological photometric detection system. Subsequently, the mass spectrometric pretreatment device performs a pretreatment, and the mass spectrometric detection system performs mass spectrometry. The mass spectrometric detection system performs mass spectrometry on components contained in a supernatant. A signal intensity and peak area for each of components are calculated from an obtained chromatograph. A quantitative value measured based on the immunoanalytical method is calculated for each of the components on the basis of the relative ratios of the components.
摘要翻译：一种免疫分析系统，其中在诸如患者血清的样品经由免疫预处理装置进行预处理之后，通过免疫光度检测系统对样品进行光检测。随后，质谱预处理装置进行预处理，质谱检测系统进行质谱分析。质谱检测系统对上清液中所含的组分进行质谱分析。从获得的色谱图中计算各组分的信号强度和峰面积。基于组分的相对比例，对每个组分计算基于免疫分析方法测量的定量值。

8. 发明授权

US09128070B2 Analysis device 有权
标题翻译：分析装置
公开(公告)号：US09128070B2
公开(公告)日：2015-09-08
申请号：US13580249
申请日：2011-01-14
申请人： Makoto Nogami , Katsuhiro Kanda , Shinya Ito
发明人： Makoto Nogami , Katsuhiro Kanda , Shinya Ito
IPC分类号： G01N27/62 , C12M1/42 , G01N35/00 , G01N35/02 , G01N35/10
CPC分类号： G01N35/0098 , G01N1/405 , G01N35/025 , G01N2035/1053
摘要： Disclosed is an analysis device which can analyze substances with low blood concentration with high precision without having to make the device larger. The analysis device is composed of: a specimen disk equipped with specimen containers; a reagent disk equipped with reagent containers; a first disk equipped with first containers where purification of the subject component to be measured in the specimen is carried out; a second disk equipped with second containers where purification of the sample purified in the first container is carried out; and a mass spectrometry unit which measures the specimen purified in the second container.
摘要翻译：公开了一种能够高精度地分析血液浓度低的物质的分析装置，而不必使装置更大。分析装置包括：装有标本容器的标本盘; 装有试剂容器的试剂盘; 配备有第一容器的第一盘，其中进行在样品中待测量的被检体成分的净化; 配备有第二容器的第二盘，其中在第一容器中净化的样品的纯化被执行; 以及质量分析单元，其测量在第二容器中净化的样品。

9. 发明申请

US20130130401A1 Biological Sample Pretreatment Method and Apparatus 审中-公开
标题翻译：生物样品预处理方法和装置
公开(公告)号：US20130130401A1
公开(公告)日：2013-05-23
申请号：US13704541
申请日：2011-06-10
申请人： Katsuhiro Kanda , Makoto Nogami , Shinya Ito , Izumi Waki
发明人： Katsuhiro Kanda , Makoto Nogami , Shinya Ito , Izumi Waki
IPC分类号： G01N1/34
CPC分类号： G01N1/34 , G01N1/405 , G01N2035/1053 , Y10T436/255
摘要： A sample treatment apparatus is disclosed that allows a filtrate after reaction to be supplied to a solid phase extraction treatment without temporary collection. A necessary amount of a solid phase extracting agent is added to a solid phase extraction device for conditioning. An aqueous solution is added to the extracting agent and supernatants are discarded. A filter device is attached to an opening of the extraction and a sample is added into a filter reservoir. A hemolyzing agent is added to the sample, and they are stirred sufficiently to cause disruption of blood cells. A protein-denaturing solution is added to the mixture, and they stirred sufficiently to denature proteins contained in the sample. They are centrifuged to leave aggregate in the filter device, and the filtrate is supplied to the solid phase extracting agent through a filter unit without collecting the filtrate.
摘要翻译：公开了一种样品处理装置，其允许将反应后的滤液供应至固相萃取处理而不进行临时收集。将必要量的固相萃取剂加入到用于调理的固相萃取装置中。向提取剂中加入水溶液，弃去上清液。过滤装置连接到提取物的开口，并将样品加入到过滤器容器中。将溶血剂加入到样品中，并充分搅拌以引起血细胞破裂。将蛋白质变性溶液加入到混合物中，并充分搅拌以使包含在样品中的蛋白质变性。将它们离心以将聚集体留在过滤装置中，并将滤液通过过滤装置供应至固相萃取剂，而不收集滤液。

10. 发明申请

US20120058009A1 ANALYZER 审中-公开
标题翻译：分析仪
公开(公告)号：US20120058009A1
公开(公告)日：2012-03-08
申请号：US13201659
申请日：2010-01-18
申请人： Makoto Nogami , Yuichiro Hashimoto , Izumi Waki , Katsuhiro Kanda
发明人： Makoto Nogami , Yuichiro Hashimoto , Izumi Waki , Katsuhiro Kanda
IPC分类号： G01N33/48
CPC分类号： G01N33/49 , G01N1/4055 , G01N2001/4083 , H01J49/0009 , H01J49/0031 , H01J49/0036 , H01J49/0436
摘要： An analyzer includes a pretreatment device and a mass spectrometer. The pretreatment device includes a solid phase extraction mechanism. The mass spectrometer performs mass spectrometry on a sample pretreated by the pretreatment device and then subjected to ionization. The analyzer also includes a storage unit that stores data on dependence of signal intensities of the analyte substance to be measured and the internal standard upon the concentration of a substance inhibiting ionization in the sample, and that stores data on a recovery rate. The analyzer also includes a correcting unit that corrects measurement results of the sample and the internal standard on the basis of the data stored in the storage unit.
摘要翻译：分析仪包括预处理装置和质谱仪。预处理装置包括固相提取机构。质谱仪对由预处理装置预处理的样品进行质谱分析，然后进行电离。分析仪还包括存储单元，其存储关于待测量的待分析物质的信号强度与内标的数据，所述数据在样品中抑制电离物质的浓度，并且存储关于回收率的数据。分析器还包括校正单元，其基于存储在存储单元中的数据来校正样本的测量结果和内部标准。

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