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    • 10. 发明申请
    • SEQUENCING METHODS
    • 序列方法
    • US20090325183A1
    • 2009-12-31
    • US12553928
    • 2009-09-03
    • Kai Qin LaoNeil A. Straus
    • Kai Qin LaoNeil A. Straus
    • C12Q1/68
    • C12Q1/6872C12Q2537/143C12Q2535/101
    • The present teachings provide methods and compositions for sequencing one or more target nucleic acids. High levels of multiplexing are provided by the use of an emulsion PCR comprising primer-immobilized beads. The resulting reaction products can be sequenced by any of a variety of mobility-dependent analytical techniques, such as mass spectrometry. In some embodiments, a first collection of amplification products on a first collection of beads are transferred to a second collection of beads. In some embodiments, a first collection of amplification products on a first collection of beads is amplified in a rolling circle amplification reaction. The present teachings also provide compositions, kits, and devices for performing and sequencing the products of the emulsion amplification reactions as described herein.
    • 本教导提供了用于测序一种或多种靶核酸的方法和组合物。 通过使用包含引物固定的珠粒的乳液PCR提供了高水平的复用。 所得到的反应产物可以通过各种迁移依赖性分析技术如质谱法中的任何一种进行测序。 在一些实施方案中,将第一集合珠上的扩增产物的第一集合转移到第二集合珠。 在一些实施方案中,在滚动圆扩增反应中扩增第一集合珠上的扩增产物的第一集合。 本教导还提供用于对本文所述的乳液扩增反应的产物进行和测序的组合物,试剂盒和装置。