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    • 4. 发明授权
    • Mesenchymal stem cells (MSC) expansion methods and materials
    • 间充质干细胞(MSC)扩增方法和材料
    • US09220810B2
    • 2015-12-29
    • US13323475
    • 2011-12-12
    • Teng MaJunho Kim
    • Teng MaJunho Kim
    • C12N5/02A61L27/38C12N5/00C12N5/0775
    • A61L27/3804A61L27/3895C12N5/0062C12N5/0663C12N2500/02C12N2533/90C12N2539/10
    • The subject invention concerns materials and methods for growing and expanding MSC while maintaining their undifferentiated phenotype, self-renewal ability, and/or multi-lineage potential. In one embodiment, a method of the invention comprises i) seeding freshly isolated MSC on a planar surface or a 3-D scaffold and growing the cells under physiological or low O2 tension for a period of time sufficient to support formation of 3-D ECM network; ii) decellularizing the planar surface or 3-D scaffold; and iii) reseeding the decellularized planar surface or 3-D scaffold with MSCs, whereby the reseeded MSCs can be grown on the scaffold and maintain an undifferentiated phenotype. In one embodiment, the 3-D scaffold comprises or is composed of PET. In one embodiment, the MSC are human MSC (hMSC).
    • 本发明涉及用于生长和扩增MSC同时保持其未分化表型,自我更新能力和/或多谱系潜力的材料和方法。 在一个实施方案中,本发明的方法包括:i)在平坦表面或3-D支架上接种新鲜分离的MSC,并在生理或低O 2张力下培养细胞足以支持3-D ECM形成的时间 网络; ii)使平面表面或3-D支架脱细胞; 和iii)用MSCs再剥离脱细胞的平面或3-D支架,由此可将重新植入的MSC植入支架上并保持未分化的表型。 在一个实施方案中,3-D支架包含PET或由PET组成。 在一个实施例中,MSC是人MSC(hMSC)。
    • 6. 发明申请
    • Multimedia Contents Processing Method And System
    • 多媒体内容处理方法与系统
    • US20120173674A1
    • 2012-07-05
    • US13242519
    • 2011-09-23
    • Junho KimJungin Han
    • Junho KimJungin Han
    • G06F15/16
    • H04L65/602H04L65/4084
    • Embodiments provide a multimedia content processing method and a multimedia content processing system. The multimedia content processing method includes transmitting, by a mobile device, a request for a multimedia content file to a host device, and generating, by the host device, a pre-processed multimedia content file in response to the request. The generating step includes performing one or more pre-process operations on the multimedia content file such that the multimedia content file is modified. The method further includes downloading, by the mobile device, the pre-processed multimedia content file from the host device, and playing, by the mobile device, the pre-processed multimedia content file.
    • 实施例提供一种多媒体内容处理方法和多媒体内容处理系统。 多媒体内容处理方法包括:通过移动设备向主机设备发送对多媒体内容文件的请求,并且由主机设备响应于该请求生成预处理的多媒体内容文件。 所述生成步骤包括对所述多媒体内容文件执行一个或多个预处理操作,使得所述多媒体内容文件被修改。 该方法还包括由移动设备从主机设备下载预处理的多媒体内容文件,以及由移动设备播放预处理的多媒体内容文件。
    • 8. 发明申请
    • MESENCHYMAL STEM CELLS (MSC) EXPANSION METHODS AND MATERIALS
    • MESENCHYMAL STEM CELLS(MSC)扩展方法和材料
    • US20120178159A1
    • 2012-07-12
    • US13323475
    • 2011-12-12
    • TENG MAJunho Kim
    • TENG MAJunho Kim
    • C12N5/077C12N5/02C12N5/0775
    • A61L27/3804A61L27/3895C12N5/0062C12N5/0663C12N2500/02C12N2533/90C12N2539/10
    • The subject invention concerns materials and methods for growing and expanding MSC while maintaining their undifferentiated phenotype, self-renewal ability, and/or multi-lineage potential. In one embodiment, a method of the invention comprises i) seeding freshly isolated MSC on a planar surface or a 3-D scaffold and growing the cells under physiological or low O2 tension for a period of time sufficient to support formation of 3-D ECM network; ii) decellularizing the planar surface or 3-D scaffold; and iii) reseeding the decellularized planar surface or 3-D scaffold with MSCs, whereby the reseeded MSCs can be grown on the scaffold and maintain an undifferentiated phenotype. In one embodiment, the 3-D scaffold comprises or is composed of PET. In one embodiment, the MSC are human MSC (hMSC).
    • 本发明涉及用于生长和扩增MSC同时保持其未分化表型,自我更新能力和/或多谱系潜力的材料和方法。 在一个实施方案中,本发明的方法包括:i)在平坦表面或3-D支架上接种新鲜分离的MSC,并在生理或低O 2张力下培养细胞足以支持3-D ECM形成的时间 网络; ii)使平面表面或3-D支架脱细胞; 和iii)用MSCs再剥离脱细胞的平面或3-D支架,由此可将重新植入的MSC植入支架上并保持未分化的表型。 在一个实施方案中,3-D支架包含PET或由PET组成。 在一个实施例中,MSC是人MSC(hMSC)。