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    • 1. 发明授权
    • Mesenchymal stem cells (MSC) expansion methods and materials
    • 间充质干细胞(MSC)扩增方法和材料
    • US09220810B2
    • 2015-12-29
    • US13323475
    • 2011-12-12
    • Teng MaJunho Kim
    • Teng MaJunho Kim
    • C12N5/02A61L27/38C12N5/00C12N5/0775
    • A61L27/3804A61L27/3895C12N5/0062C12N5/0663C12N2500/02C12N2533/90C12N2539/10
    • The subject invention concerns materials and methods for growing and expanding MSC while maintaining their undifferentiated phenotype, self-renewal ability, and/or multi-lineage potential. In one embodiment, a method of the invention comprises i) seeding freshly isolated MSC on a planar surface or a 3-D scaffold and growing the cells under physiological or low O2 tension for a period of time sufficient to support formation of 3-D ECM network; ii) decellularizing the planar surface or 3-D scaffold; and iii) reseeding the decellularized planar surface or 3-D scaffold with MSCs, whereby the reseeded MSCs can be grown on the scaffold and maintain an undifferentiated phenotype. In one embodiment, the 3-D scaffold comprises or is composed of PET. In one embodiment, the MSC are human MSC (hMSC).
    • 本发明涉及用于生长和扩增MSC同时保持其未分化表型,自我更新能力和/或多谱系潜力的材料和方法。 在一个实施方案中,本发明的方法包括:i)在平坦表面或3-D支架上接种新鲜分离的MSC,并在生理或低O 2张力下培养细胞足以支持3-D ECM形成的时间 网络; ii)使平面表面或3-D支架脱细胞; 和iii)用MSCs再剥离脱细胞的平面或3-D支架,由此可将重新植入的MSC植入支架上并保持未分化的表型。 在一个实施方案中,3-D支架包含PET或由PET组成。 在一个实施例中,MSC是人MSC(hMSC)。
    • 2. 发明申请
    • BEARD NECKLINE GUIDE APPARATUS AND METHOD
    • US20200069026A1
    • 2020-03-05
    • US16356867
    • 2019-03-18
    • Teng Ma
    • Teng Ma
    • A45D24/36
    • A beard trimming template is provided comprising a first elongated portion formed from a first straight edge having a first length, a and a second straight edge having a second length, the first and second straight edges positioned parallel to each other. A second elongated portion having a third straight edge and a fourth straight edge, the third and fourth straight edges positioned parallel to each other.A main body is formed by a radius bridging ends of the second and third straight edges proximal to the main body, and an apex, opposite the radius, formed by ends of the first and fourth straight edges proximal to the main body joining at a right angle. The first and second straight edges are connected via a fifth straight edge forming a first width edge between the first and second straight edges and the third and fourth straight edges are connected via a sixth straight edge forming a second width edge, and wherein the first and second width edges are equal in dimension and are distal to the main body.
    • 4. 发明授权
    • Modular cell culture bioreactor and associated methods
    • 模块化细胞培养生物反应器及相关方法
    • US06875605B1
    • 2005-04-05
    • US10645350
    • 2003-08-21
    • Teng Ma
    • Teng Ma
    • C12N5/02C12N5/077C12N5/0789
    • C12N5/0663C12N5/0647C12N2533/30
    • An apparatus and method for a modular cell culture bioreactor comprises a plurality of chambers for cell culture; at least one reservoir containing a cell support medium; a plurality of conduits fluidly connecting the at least one reservoir with the plurality of chambers; and at least one pump fluidly connected through the plurality of conduits with the at least one reservoir and with the plurality of chambers to pump cell support medium therethrough; wherein each individual chamber of the plurality of chambers includes at least one three-dimensional matrix comprising polyethylene terephthalate, a plurality of channels carrying the cell support medium and having the matrix positioned in fluid communication therebetween, and at least two openings into each the channel, wherein a first the opening is in fluid connection with the pump and a second the opening is in fluid connection with the reservoir.
    • 用于模块化细胞培养生物反应器的装置和方法包括用于细胞培养的多个室; 至少一个包含细胞支持介质的储存器; 将所述至少一个储存器与所述多个室流体连接的多个导管; 以及至少一个泵,其通过所述多个管道与所述至少一个储存器流体连接并且与所述多个室流体连接以通过其泵送单元支撑介质; 其中所述多个腔室中的每个单独腔室包括​​至少一个三维矩阵,所述三维矩阵包括聚对苯二甲酸乙二醇酯,多个通道,其承载所述细胞支持介质并且具有定位在其间流体连通的所述基质,以及每个所述通道中的至少两个开口, 其中第一开口与泵流体连接,第二开口与贮液器流体连通。
    • 8. 发明授权
    • Predictive position encoding
    • 预测位置编码
    • US09111333B2
    • 2015-08-18
    • US14356233
    • 2011-11-07
    • Wenfei JiangKangying CaiTeng Ma
    • Wenfei JiangKangying CaiTeng Ma
    • G06T9/00G06T17/00G06T17/20G06T9/40
    • G06T9/001G06T9/005G06T9/40G06T17/005G06T17/205G06T2207/20021
    • A method and apparatus for position coding of three dimensional mesh models are described including estimating a symbol probability of a non-empty-child-cell Cl,k, where Ql,k denotes the kth cell at layer l, wherein the symbol probability is estimated based on an accuracy of a fitted plane P, sub-dividing the non-empty-child-cell to produce a sub-cell, if the non-empty-child-cell has more than one vertex, determining if there are more unprocessed non-empty-child-cells at layer l, if there are no more unprocessed non-empty-child-cells at layer l, determining if all non-empty-child-cells at layer l have only one vertex and a distance between the center of the sub-cell and a point inside the sub-cell is less than or equal to a first threshold and entropy coding symbols representing a position of the non-empty-child-cells, if all non-empty-child-cells at layer l have only one vertex and the distance between the center of the sub-cell and the point inside the sub-cell is less than or equal to the first threshold.
    • 描述了用于三维网格模型的位置编码的方法和装置,包括估计非空子单元Cl的k的符号概率,其中,Q1,k表示第l层的第k个单元,其中估计符号概率 基于拟合平面P的精度,如果非空子子单元具有多于一个顶点,则将非空子单元分割以产生子单元,确定是否存在更多未处理的非单元, 如果层l上没有更多未处理的非空子小区,则确定层l上的所有非空小子单元是否只有一个顶点和中心之间的距离 并且子单元内的点小于或等于第一阈值,并且如果在层上的所有非空子子单元都表示非空子子单元的位置,则熵编码表示非空子单元的位置的符号 l只有一个顶点,子单元的中心与子单元内的点之间的距离小于或等于 到第一个阈值。
    • 9. 发明申请
    • PREDICTIVE POSITION ENCODING
    • 预测位置编码
    • US20140376827A1
    • 2014-12-25
    • US14356233
    • 2011-11-07
    • Wenfei JiangKangying CaiTeng Ma
    • Wenfei JiangKangying CaiTeng Ma
    • G06T9/00G06T9/40
    • G06T9/001G06T9/005G06T9/40G06T17/005G06T17/205G06T2207/20021
    • A method and apparatus for position coding of three dimensional mesh models are described including estimating a symbol probability of a non-empty-child-cell Cl,k, where Ql,k denotes the kth cell at layer l, wherein the symbol probability is estimated based on an accuracy of a fitted plane P, sub-dividing the non-empty-child-cell to produce a sub-cell, if the non-empty-child-cell has more than one vertex, determining if there are more unprocessed non-empty-child-cells at layer l, if there are no more unprocessed non-empty-child-cells at layer l, determining if all non-empty-child-cells at layer l have only one vertex and a distance between the center of the sub-cell and a point inside the sub-cell is less than or equal to a first threshold and entropy coding symbols representing a position of the non-empty-child-cells, if all non-empty-child-cells at layer l have only one vertex and the distance between the center of the sub-cell and the point inside the sub-cell is less than or equal to the first threshold.
    • 描述了用于三维网格模型的位置编码的方法和装置,包括估计非空子单元Cl的k的符号概率,其中,Q1,k表示第l层的第k个单元,其中估计符号概率 基于拟合平面P的精度,如果非空子子单元具有多于一个顶点,则将非空子单元分割以产生子单元,确定是否存在更多未处理的非单元, 如果层l上没有更多未处理的非空子小区,则确定层l上的所有非空小子单元是否只有一个顶点和中心之间的距离 并且子单元内的点小于或等于第一阈值,并且如果在层上的所有非空子子单元都表示非空子子单元的位置,则熵编码表示非空子单元的位置的符号 l只有一个顶点,子单元的中心与子单元内的点之间的距离小于或等于 到第一个阈值。
    • 10. 发明授权
    • Modular cell culture bioreactor
    • 模块细胞培养生物反应器
    • US07122371B1
    • 2006-10-17
    • US11093956
    • 2005-03-30
    • Teng Ma
    • Teng Ma
    • C12N5/02
    • C12N5/0663C12N5/0647C12N2533/30
    • An apparatus and method for a modular cell culture bioreactor comprises a plurality of chambers for cell culture; at least one reservoir containing a cell support medium; a plurality of conduits fluidly connecting the at least one reservoir with the plurality of chambers; and at least one pump fluidly connected through the plurality of conduits with the at least one reservoir and with the plurality of chambers to pump cell support medium therethrough; wherein each individual chamber of the plurality of chambers includes at least one three-dimensional matrix comprising polyethylene terephthalate, a plurality of channels carrying the cell support medium and having the matrix positioned in fluid communication therebetween, and at least two openings into each the channel, wherein a first the opening is in fluid connection with the pump and a second the opening is in fluid connection with the reservoir.
    • 用于模块化细胞培养生物反应器的装置和方法包括用于细胞培养的多个室; 至少一个包含细胞支持介质的储存器; 将所述至少一个储存器与所述多个室流体连接的多个导管; 以及至少一个泵,其通过所述多个管道与所述至少一个储存器流体连接并且与所述多个室流体连接以通过其泵送单元支撑介质; 其中所述多个腔室中的每个单独腔室包括​​至少一个三维矩阵,所述三维矩阵包括聚对苯二甲酸乙二醇酯,多个通道,其承载所述细胞支持介质并且具有定位在其间流体连通的所述基质,以及每个所述通道中的至少两个开口, 其中第一开口与泵流体连接,第二开口与贮液器流体连通。