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    • 5. 发明申请
    • Regulating the ethylene response of a plant by modulation of F-box proteins
    • 通过调节F盒蛋白来调节植物的乙烯应答
    • US20060200875A1
    • 2006-09-07
    • US11298286
    • 2005-12-09
    • Hongwei GuoJoseph Ecker
    • Hongwei GuoJoseph Ecker
    • A01H1/00C12N15/82
    • C12N15/8291C12N15/8249C12N15/8261C12N15/8266C12N15/8267C12N15/8271
    • The relationship between F-box proteins and proteins involved in the ethylene response in plants is described. In particular, F-box proteins may bind to proteins involved in the ethylene response and target them for degradation by the ubiquitin/proteasome pathway. The transcription factor EIN3 is a key transcription factor mediating ethylene-regulated gene expression and morphological responses. EIN3 is degraded through a ubiquitin/proteasome pathway mediated by F-box proteins EBF1 and EBF2. The link between F-box proteins and the ethylene response is a key step in modulating or regulating the response of a plant to ethylene. Described herein are transgenic plants having an altered sensitivity to ethylene, and methods for making transgenic plant having an altered sensitivity to ethylene by modulating the level of activity of F-box proteins. Methods of altering the ethylene response in a plant by modulating the activity or expression of an F-box protein are described. Also described are methods of identifying compounds that modulate the ethylene response in plants by modulating the level of F-box protein expression or activity.
    • 描述了F-box蛋白和参与植物乙烯应答的蛋白质之间的关系。 特别地,F盒蛋白可能与参与乙烯应答的蛋白结合,并将其靶向通过泛素/蛋白酶体途径降解。 转录因子EIN3是介导乙烯调节基因表达和形态学反应的关键转录因子。 EIN3通过F-box蛋白EBF1和EBF2介导的泛素/蛋白酶体途径降解。 F-box蛋白与乙烯反应之间的联系是调节或调节植物对乙烯的反应的关键步骤。 本文描述了对乙烯具有改变的敏感性的转基因植物,以及通过调节F-box蛋白的活性水平来制备对乙烯具有改变的敏感性的转基因植物的方法。 描述了通过调节F盒蛋白的活性或表达来改变植物中的乙烯应答的方法。 还描述了通过调节F-box蛋白表达或活性的水平鉴定调节植物中乙烯应答的化合物的方法。