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1. 发明授权

US6063916A Transesterification of insoluble polysaccharides 失效
标题翻译：不溶性多糖的酯交换
公开(公告)号：US6063916A
公开(公告)日：2000-05-16
申请号：US774329
申请日：1996-11-27
申请人： Joseph A. Akkara , David L. Kaplan , Ferdinando F. Bruno , Jonathan S. Dordick
发明人： Joseph A. Akkara , David L. Kaplan , Ferdinando F. Bruno , Jonathan S. Dordick
IPC分类号： C12P7/62 , C08B3/00 , C08B31/02 , C08B37/00
CPC分类号： C12P7/625
摘要： Bacillus subtilis protease catalyzes the acylation of organic solvent-insoluble polysaccharides in isooctane solution containing vinyl esters of fatty acids as acyl donor. The reaction occurs only when the enzyme is solubilized via ion-pairing with the anionic surfactant dioctyl sulfosuccinate, sodium salt (AOT). Enzyme based acylation was demonstrated with amylose, cyclodextrins, cellulose, cellulose derivatives, and other polysaccharides such as chitosan, pullulan, and maltodextrose. These polysaccharides are reactive either as a cryogenically milled powder suspended in the organic solvent or as a thin film deposited onto ZnSe slides. For chitosan, .alpha.-cyclodextrin, and hydroxyethyl cellulose (HEC), the enzymatic crosslinking reaction occurs using adipic acid divinyl ester (C6DVE). HEC forms a compound that gels in solvents such as ethyl alcohol and dimethyl sulfone oxide (DMSO). Electron spectroscopy chemical analysis (ESCA) of the first 100 .ANG. of the amylose thin film amylose indicates that the acylated surface had a degree of substitution of 0.9.+-.0.1 acyl chains per glucose moiety and this corresponded well to the expected regioselectivity of subtilisin catalysis on glucose-containing compounds. .sup.1 H-NMR studies indicated that only the C-6 hydroxyl groups of the glucose moiety were acylated with amylose and .gamma.-cyclodextrin. However, .beta.-cyclodextrin, and .alpha.-cyclodextrin were modified at secondary alcohols and at all three alcohols, respectively. This approach represents the first attempt at using enzymes to modify organic solvent-insoluble polymers in nonaqueous media.
摘要翻译：枯草芽孢杆菌蛋白酶催化有机溶剂不溶性多糖在含有作为酰基供体的脂肪酸乙烯基酯的异辛烷溶液中的酰化。仅当酶通过离子配对与阴离子表面活性剂二辛基磺基琥珀酸钠（AOT）溶解时才发生反应。用直链淀粉，环糊精，纤维素，纤维素衍生物和其它多糖例如壳聚糖，支链淀粉和麦芽糊精证明酶基酰化。这些多糖作为悬浮在有机溶剂中的低温研磨粉末或沉积在ZnSe载片上的薄膜是反应性的。对于壳聚糖，α-环糊精和羟乙基纤维素（HEC），使用己二酸二乙烯基酯（C 6 DVE）发生酶交联反应。 HEC形成在溶剂如乙醇和二甲基砜氧化物（DMSO）中凝胶化合物。直链淀粉薄膜直链淀粉的前100个ANGSTROM的电子光谱化学分析（ESCA）表明，酰化表面的每个葡萄糖部分的取代度为0.9 +/- 0.1个酰基链，这与枯草杆菌蛋白酶催化的预期区域选择性相当对含葡萄糖的化合物。 1 H-NMR研究表明，只有葡萄糖部分的C-6羟基被直链淀粉和γ-环糊精酰化。然而，β-环糊精和α-环糊精分别在仲醇和所有三种醇上进行了改性。该方法代表了使用酶来修饰非水介质中有机溶剂不溶性聚合物的第一次尝试。

2. 发明授权

US06228997B1 Transesterification of insoluble polysaccharides 失效
标题翻译：不溶性多糖的酯交换
公开(公告)号：US06228997B1
公开(公告)日：2001-05-08
申请号：US09192209
申请日：1998-07-10
申请人： Joseph A. Akkara , David L. Kaplan , Ferdinando F. Bruno , Jonathan S. Dordick
发明人： Joseph A. Akkara , David L. Kaplan , Ferdinando F. Bruno , Jonathan S. Dordick
IPC分类号： C08B3708
CPC分类号： C12P7/62 , C08B11/20 , C08B37/00 , C08B37/0012 , C08B37/003 , C12P19/04
摘要： Bacillus subtilis protease catalyzes the acylation of organic solvent-insoluble polysaccharides in isooctane solution containing vinyl esters of fatty acids as acyl donor. The reaction occurs only when the enzyme is solubilized via ion-pairing with the anionic surfactant dioctyl sulfosuccinate, sodium salt (AOT). Enzyme based acylation was demonstrated with amylose, cyclodextrins, cellulose, cellulose derivatives, and other polysaccharides such as chitosan, pullulan, and maltodextrose. These polysaccharides are reactive either as a cryogenically milled powder suspended in the organic solvent or as a thin film deposited onto ZnSe slides. For chitosan, &agr;-cyclodextrin, and hydroxyethyl cellulose (HEC), the enzymatic crosslinking reaction occurs using adipic acid divinyl ester (C6DVE). HEC forms a compound that gels in solvents such as ethyl alcohol and dimethyl sulfone oxide (DMSO). Electron spectroscopy chemical analysis (ESCA) of the first 100 Å of the amylose thin film amylose indicates that the acylated surface had a degree of substitution of 0.9±0.1 acyl chains per glucose moiety and this corresponded well to the expected regioselectivity of subtilisin catalysis on glucose-containing compounds. 1H-NMR studies indicated that only the C-6 hydroxyl groups of the glucose moiety were acylated with amylose and &ggr;-cyclodextrin. However, &bgr;-cyclodextrin, and &agr;-cyclodextrin were modified at secondary alcohols and at all three alcohols, respectively. This approach represents the first attempt at using enzymes to modify organic solvent-insoluble polymers in nonaqueous media.
摘要翻译：枯草芽孢杆菌蛋白酶催化有机溶剂不溶性多糖在含有作为酰基供体的脂肪酸乙烯基酯的异辛烷溶液中的酰化。仅当酶通过离子配对与阴离子表面活性剂二辛基磺基琥珀酸钠（AOT）溶解时才发生反应。用直链淀粉，环糊精，纤维素，纤维素衍生物和其它多糖例如壳聚糖，支链淀粉和麦芽糊精证明酶基酰化。这些多糖作为悬浮在有机溶剂中的低温研磨粉末或沉积在ZnSe载片上的薄膜是反应性的。对于壳聚糖，α-环糊精和羟乙基纤维素（HEC），使用己二酸二乙烯基酯（C 6 DVE）进行酶交联反应。 HEC形成在溶剂如乙醇和二甲基砜氧化物（DMSO）中凝胶化合物。直链淀粉薄膜直链淀粉的第一个100的电子光谱化学分析（ESCA）表明，酰化表面的每个葡萄糖部分具有0.9±0.1个酰基链的取代度，这与枯草杆菌蛋白酶对葡萄糖的催化的预期区域选择性相当的化合物。 1 H-NMR研究表明，只有葡萄糖部分的C-6羟基被直链淀粉和γ-环糊精酰化。然而，β-环糊精和α-环糊精分别在仲醇和所有三种醇中进行了改性。该方法代表了使用酶来修饰非水介质中有机溶剂不溶性聚合物的第一次尝试。

3. 发明授权

US5128248A Selective acrylation of sugars 失效
标题翻译：糖的选择性丙烯酸
公开(公告)号：US5128248A
公开(公告)日：1992-07-07
申请号：US412904
申请日：1989-09-26
申请人： Jonathan S. Dordick , Andrew J. Hacking , Riaz A. Khan
发明人： Jonathan S. Dordick , Andrew J. Hacking , Riaz A. Khan
IPC分类号： C12P19/12 , C07H5/02 , C07H13/04 , C07H13/06 , C07H13/08
CPC分类号： C12P19/12 , C07H13/04 , C07H13/08 , C07H5/02
摘要： Sucrose or derivatives thereof such as esters are acylated at the 4'- and/or the 6-position by reaction with a donor acyl ester, e.g. a reactive ester of an alkanoic acid or benzoic acid, in the presence of lipase. Sucrose 6,4'-diesters so produced can be chlorinated and deacylated to produce the sweetener sucralose.
摘要翻译：蔗糖或其衍生物如酯通过与供体酰基酯反应而在4'-和/或6-位被酰化。在脂肪酶存在下的链烷酸或苯甲酸的反应性酯。如此生产的蔗糖6,4'-二酯可以被氯化和脱酰基化以产生甜味剂三氯蔗糖。

4. 发明授权

US4826962A Tetrachlororaffinose and its use in the preparation of sucralose 失效
标题翻译：四氯丝氨酸及其在制备三氯蔗糖中的应用
公开(公告)号：US4826962A
公开(公告)日：1989-05-02
申请号：US921370
申请日：1986-10-20
申请人： Elner B. Rathbone , Khizar S. Mufti , Riaz A. Khan , Peter S. J. Cheetham , Andrew J. Hacking , Jonathan S. Dordick
发明人： Elner B. Rathbone , Khizar S. Mufti , Riaz A. Khan , Peter S. J. Cheetham , Andrew J. Hacking , Jonathan S. Dordick
IPC分类号： A23G3/38 , A23G3/34 , C07H1/00 , C07H3/04 , C07H5/02 , C12P19/00 , C12P19/14 , C12P19/20 , C12R1/645 , C12R1/685
CPC分类号： C07H5/02
摘要： The novel chlorinated sugar O-.alpha.-D-6-chloro-6-deoxygalactopyranosyl-(1.fwdarw.6)-.alpha.-D-4-chloro-4-deoxygalactopyranosyl-(1.fwdarw.2)-.beta.-D-1, 6-dichloro-1, 6-dideoxyfructofuranoside (TCR) can be used to prepare sucralose by incubating the TCR in solution in the presence of an enzyme serving to remove the 6-chloro-6-deoxygalactosyl moiety from the 6-position, especially an enzyme derived from a strain of Mortierella vinacea, Circinella muscae or Aspergillus niger. TCR is prepared by treating raffinose with thionyl chloride in the presence of triphenylphosphine oxide.
摘要翻译：新型氯化糖O-α-D-6-氯-6-脱氧半乳糖吡喃糖基 - （1-> 6）-α-D-4-氯-4-脱氧半乳糖吡喃糖基 - （1-> 2）-β-D-1， 6-二氯-1,6-二脱氧呋喃果糖苷（TCR）可用于制备三氯蔗糖，通过在用于从6-位除去6-氯-6-脱氧半乳糖基部分的酶存在下，在溶液中温育TCR，特别是衍生自高山被孢霉菌株，，蛇科或黑曲霉的酶。通过在三苯基氧化膦存在下用亚硫酰氯处理棉子糖来制备TCR。

5. 发明授权

US09562225B2 Internalization of proteins into hollowed gold nanostructures 有权
标题翻译：将蛋白质内化为中空金纳米结构
公开(公告)号：US09562225B2
公开(公告)日：2017-02-07
申请号：US14676258
申请日：2015-04-01
申请人： Xi Qian , Jennifer E. Gagner , Jonathan S. Dordick , Richard W. Siegel
发明人： Xi Qian , Jennifer E. Gagner , Jonathan S. Dordick , Richard W. Siegel
IPC分类号： C12N11/14 , C12N9/36 , C23C18/16 , B82Y40/00 , C23C18/54 , A61K47/48
CPC分类号： C12N11/14 , A61K47/48861 , A61K47/48884 , A61K47/6923 , A61K47/6929 , B82Y5/00 , B82Y30/00 , B82Y40/00 , C12N9/2462 , C12Y302/01017 , C12Y304/21001 , C23C18/1635 , C23C18/1657 , C23C18/54
摘要： Provided is a gold nanocage with pores, charged ligand molecules covalently bound to internal surfaces of the gold nanocage, and payload molecules electrostatically adsorbed onto said charged ligand molecules, wherein a pore diameter is between 1.2 and 20 times a gyration radius of the payload molecule. Also provided is a method for making a nanoparticle, including using polyvinylpyrrolidone as a capping agent in a galvanic replacement reaction to convert a silver nanocube into a gold nanocage having pores, replacing the polyvinylpyrrolidone on internal surfaces with charged ligand molecules, and electrostatically adsorbing payload molecules onto the charged ligand molecules, with a pore diameter less than twenty times a gyration radius of the payload molecule. Also provided is a method of delivering a pharmacological agent to a mammalian cell, including contacting the cell with a gold nanocage having pores, ligand molecules bound to internal surfaces, and pharmacological agent adsorbed onto ligand molecules.
摘要翻译：提供了具有孔的金纳米笼，带电的配体分子共价结合到金纳米笼的内表面，以及静电吸附到所述带电配位体分子上的有效载荷分子，其中孔直径为有效载荷分子的回转半径的1.2至20倍。还提供了一种制备纳米颗粒的方法，包括在电替换反应中使用聚乙烯吡咯烷酮作为封端剂将银纳米管转化成具有孔的金纳米笼，用带电的配体分子代替内表面上的聚乙烯吡咯烷酮，以及静电吸附有效载体分子带电的配体分子，其孔径小于有效载荷分子的回转半径的二十倍。还提供了将药理学递送到哺乳动物细胞的方法，包括使细胞与具有孔的金纳米笼，结合到内表面的配体分子和吸附在配体分子上的药理学接触。

6. 发明授权

US08883452B2 K5 heparosan fermentation and purification 有权
标题翻译： K5肝炎发酵和纯化
公开(公告)号：US08883452B2
公开(公告)日：2014-11-11
申请号：US13261193
申请日：2010-08-30
申请人： Zhenyu Wang , Robert J. Linhardt , Jonathan S. Dordick , Ujjwal Bhaskar
发明人： Zhenyu Wang , Robert J. Linhardt , Jonathan S. Dordick , Ujjwal Bhaskar
IPC分类号： C08B37/10 , C12P19/28 , C08B37/00 , C12P19/26
CPC分类号： C08B37/0063 , C08B37/0003 , C12P19/26
摘要： A method for the production of heparosan from fermentation culture of E. coli K5 suitable for industrial production, exhibiting superior yield and purity, smaller culture volumes, faster growth, and lower costs.
摘要翻译：一种适用于工业生产的大肠杆菌K5发酵培养生产肝素的方法，具有优异的产量和纯度，较小的培养体积，更快的生长和更低的成本。

7. 发明申请

US20120301870A1 Enhanced Stability of Proteins Immobilized on Nanoparticles 有权
标题翻译：固定在纳米颗粒上的蛋白质的增强的稳定性
公开(公告)号：US20120301870A1
公开(公告)日：2012-11-29
申请号：US13456571
申请日：2012-04-26
申请人： Jonathan S. Dordick , Ravindra S. Kane , Prashanth Asuri , Sandeep S. Karajanagi , Alexey A. Vertegel , Richard W. Siegel
发明人： Jonathan S. Dordick , Ravindra S. Kane , Prashanth Asuri , Sandeep S. Karajanagi , Alexey A. Vertegel , Richard W. Siegel
IPC分类号： C12N11/00 , C12Q1/25 , C12N11/14 , C12N11/08 , C12N9/96 , C12S9/00 , B82Y15/00
CPC分类号： G01N33/54346 , B82Y15/00 , C12Q1/001 , G01N33/587 , G01N33/588
摘要： This invention is directed to the application of a previously unknown property of nanomaterials—its ability to enhance protein activity and stability at high temperatures, in organic solvents, and in polymer composites. Nanomaterials such as single-walled carbon nanotubes (SWNTs) can significantly enhance enzyme function and stability in strongly denaturing environments. Experimental results and theoretical analysis reveal that the enhancement in stability is a result of the curvature of these nanoscale materials, which suppresses unfavorable protein-protein interactions.
摘要翻译：本发明涉及以前未知的纳米材料性质的应用 - 其在高温下，有机溶剂和聚合物复合材料中增强蛋白质活性和稳定性的能力。纳米材料如单壁碳纳米管（SWNT）可以显着增强强力变性环境中的酶功能和稳定性。实验结果和理论分析表明，稳定性的提高是这些纳米材料的曲率的结果，其抑制不利的蛋白质 - 蛋白质相互作用。

8. 发明申请

US20120157669A1 K5 HEPAROSAN FERMENTATION AND PURIFICATION 有权
标题翻译： K5 HEPAROSAN发酵和纯化
公开(公告)号：US20120157669A1
公开(公告)日：2012-06-21
申请号：US13261193
申请日：2010-08-30
申请人： Zhenyu Wang , Robert J. Linhardt , Jonathan S. Dordick , Ujjwal Bhaskar
发明人： Zhenyu Wang , Robert J. Linhardt , Jonathan S. Dordick , Ujjwal Bhaskar
IPC分类号： C08B37/10 , C12P19/28
CPC分类号： C08B37/0063 , C08B37/0003 , C12P19/26
摘要： A method for the production of heparosan from fermentation culture of E. coli K5 suitable for industrial production, exhibiting superior yield and purity, smaller culture volumes, faster growth, and lower costs.
摘要翻译：一种适用于工业生产的大肠杆菌K5发酵培养生产肝素的方法，具有优异的产量和纯度，较小的培养体积，更快的生长和更低的成本。

9. 发明申请

US20110190402A1 SYNTHETIC WOOD COMPOSITE 有权
标题翻译：合成木复合材料
公开(公告)号：US20110190402A1
公开(公告)日：2011-08-04
申请号：US12849340
申请日：2010-08-03
申请人： Robert J. Linhardt , Jonathan S. Dordick , Trevor J. Simmons , Minoru Miyauchi , Sang-Hyun Lee
发明人： Robert J. Linhardt , Jonathan S. Dordick , Trevor J. Simmons , Minoru Miyauchi , Sang-Hyun Lee
IPC分类号： A01N25/00 , C09D101/02 , B05D3/02 , A01P1/00 , B82Y30/00
CPC分类号： A01N25/00 , B05D3/02 , B82Y30/00 , C09D101/02
摘要： The invention is directed a synthetic wood composite comprising biomimetic macromolecules and methods for the preparation thereof.
摘要翻译：本发明涉及包含仿生大分子的合成木材复合材料及其制备方法。

10. 发明授权

US07427497B2 In vitro metabolic engineering on microscale devices 有权
标题翻译：微量装置上的体外代谢工程
公开(公告)号：US07427497B2
公开(公告)日：2008-09-23
申请号：US10287440
申请日：2002-11-01
申请人： Jonathan S. Dordick , Aravind Srinivasan , Jungbae Kim , David H. Sherman , Douglas S. Clark
发明人： Jonathan S. Dordick , Aravind Srinivasan , Jungbae Kim , David H. Sherman , Douglas S. Clark
IPC分类号： C12N11/18 , C12N1/00
CPC分类号： B01J19/0093 , B01J19/0046 , B01J2219/00317 , B01J2219/00364 , B01J2219/00497 , B01J2219/00527 , B01J2219/00533 , B01J2219/00536 , B01J2219/00585 , B01J2219/00596 , B01J2219/00644 , B01J2219/00691 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00743 , B01J2219/00835 , B01J2219/00837 , B01J2219/0086 , B01L3/5027 , B01L3/5085 , B01L2300/069 , B01L2300/0819 , B01L2300/0829
摘要： Disclosed herein is a microfluidics device that can be used to prepare natural products and their analogs. The device comprises the enzymes of a biosynthetic pathway immobilized thereon and a means for sequentially directing a starting material and each ensuing reaction product to the enzymes of the biosynthetic pathway in the order corresponding to the steps of the biosynthetic pathway. The device can thus be used to prepare the natural product using the natural starting material of the biosynthetic pathway or analogs of the natural product using an unnatural starting material. Alternatively, artificial pathways can be created by immobilizing an appropriate selection of enzymes on the device in an order whereby each subsequent enzyme can catalyze a reaction with the product of the prior enzyme. Novel chemical entities can be prepared from these artificial pathways.
摘要翻译：本文公开了可用于制备天然产物及其类似物的微流体装置。该装置包括固定在其上的生物合成途径的酶，以及以与生物合成途径的步骤相应的顺序将原料和每个随后的反应产物顺序地引导到生物合成途径的酶的装置。因此，该装置可以用于使用天然起始物质的天然产物或天然产物的类似物使用非天然原料制备天然产物。或者，可以通过以适当顺序的酶固定适当选择的酶在装置上来产生人工途径，其中每个随后的酶可以催化与先前酶的产物的反应。这些人造途径可以制备新的化学物质。

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