会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 9. 发明申请
    • Methods and compositions to diagnose disease using concatenated oligopeptide standard
    • 使用连接寡肽标准诊断疾病的方法和组合物
    • US20060211077A1
    • 2006-09-21
    • US11082348
    • 2005-03-17
    • Kenneth AbelAndreas BraunJohanna WhitacreKai Tang
    • Kenneth AbelAndreas BraunJohanna WhitacreKai Tang
    • C12Q1/37G01N33/00
    • C12Q1/37
    • The present invention is a method to quantitate proteins associated with a disease or disease state comprising the steps of isolating at least two target proteins from a test sample, combining the target proteins with a known concentration of a concatenated oligopeptide standard and a proteinase to form peptide fragments and standard peptide fragments. The concatenated oligopeptide standard comprising at least one peptide having an amino acid sequence substantially similar to a unique amino acid sequence of a peptide fragment of the two target proteins. The peptides for each of the target proteins are joined by proteinase cleavage sites to form the concatenated oligopeptide. Quantitation of the target proteins is determined by comparison of the peptide fragments to the standard peptide fragments using mass spectroscopy.
    • 本发明是一种定量与疾病或疾病状态相关的蛋白质的方法,包括以下步骤:从测试样品中分离出至少两种靶蛋白,将目标蛋白质与已知浓度的连接寡肽标准品和蛋白酶组合以形成肽 片段和标准肽片段。 连接的寡肽标准品包含至少一种具有与两种靶蛋白的肽片段的独特氨基酸序列基本相似的氨基酸序列的肽。 用于每种靶蛋白的肽通过蛋白酶切割位点连接以形成连接的寡肽。 通过使用质谱法将肽片段与标准肽片段进行比较来确定靶蛋白质的定量。