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1. 发明授权

US09790534B2 Methods for separation, characterization and/or identification of microorganisms using spectroscopy 有权
公开(公告)号：US09790534B2
公开(公告)日：2017-10-17
申请号：US14146367
申请日：2014-01-02
申请人： John Walsh , Jones Hyman , Thurman Thorpe , Bradford Clay , Christopher Ronsick
发明人： John Walsh , Jones Hyman , Thurman Thorpe , Bradford Clay , Christopher Ronsick
IPC分类号： C12Q1/04 , G01N33/68 , H01J49/00
CPC分类号： C12Q1/04 , G01N33/6848 , H01J49/00
摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said spectroscopic measurements.

2. 发明授权

US08795983B2 Methods for the characterization of microorganisms on solid or semi-solid media 有权
标题翻译：在固体或半固体培养基上表征微生物的方法
公开(公告)号：US08795983B2
公开(公告)日：2014-08-05
申请号：US13959802
申请日：2013-08-06
申请人： Jones Hyman , John Walsh , Thurman Thorpe
发明人： Jones Hyman , John Walsh , Thurman Thorpe
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04
摘要： The present invention relates to methods and systems for scanning, detecting, and monitoring microorganisms on solid or semi-solid media using intrinsic fluorescence (IF) measurements. The methods are further directed to detection, characterization and/or identification of microorganisms on a solid or semi-solid media using intrinsic fluorescence (IF) measurements that are characteristic of said microorganisms.
摘要翻译：本发明涉及使用固有荧光（IF）测量在固体或半固体培养基上扫描，检测和监测微生物的方法和系统。所述方法进一步涉及使用所述微生物特征的固有荧光（IF）测量法在固体或半固体培养基上检测，表征和/或鉴定微生物。

3. 发明授权

US10335785B2 Methods for the isolation, accumulation, characterization and/or identification of microorganisms using a filtration and sample transfer device 有权
公开(公告)号：US10335785B2
公开(公告)日：2019-07-02
申请号：US13328379
申请日：2011-12-16
申请人： John Walsh , Jones Hyman , Christopher Ronsick
发明人： John Walsh , Jones Hyman , Christopher Ronsick
IPC分类号： B01L3/00
摘要： The present invention is directed to methods, devices and kits for separating, accumulating, characterizing and/or identifying microorganisms in a test sample. The method of the invention includes optionally lysing non-microorganism cells and/or particulates that may be present in a test sample, followed by a subsequent filtration step for isolation and/or accumulation of microorganisms. The filtration and sample transfer device may be used for isolation and/or accumulation of microorganisms by filtration. Once the microorganisms have been filtered for isolation and/or accumulation, the microorganisms can be analyzed to acquire measurements for the characterization and/or identification of said microorganisms.

4. 发明申请

US20140186879A1 Methods for Separation, Characterization and/or Identification of Microorganisms using Spectroscopy 有权
标题翻译：使用光谱分离，表征和/或鉴定微生物的方法
公开(公告)号：US20140186879A1
公开(公告)日：2014-07-03
申请号：US14146367
申请日：2014-01-02
申请人： John Walsh , Jones Hyman , Thurman Thorpe , Bradford Clay , Christopher Ronsick
发明人： John Walsh , Jones Hyman , Thurman Thorpe , Bradford Clay , Christopher Ronsick
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , G01N33/6848 , H01J49/00
摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said spectroscopic measurements.
摘要翻译：本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。本发明还提供分离的微生物样品的光谱询问以产生微生物的测量，并使用所述光谱测量来表征和/或鉴定样品中的微生物。

5. 发明授权

US08647835B2 Methods for separation, characterization and/or identification of microorganisms using spectroscopy 有权
标题翻译：使用光谱法分离，表征和/或鉴定微生物的方法
公开(公告)号：US08647835B2
公开(公告)日：2014-02-11
申请号：US13117976
申请日：2011-05-27
申请人： John Walsh , Jones Hyman
发明人： John Walsh , Jones Hyman
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , G01N21/35 , G01N21/3563 , G01N21/3577 , G01N21/3581 , G01N21/47 , G01N21/64 , G01N21/6486 , G01N21/65 , G01N2015/1402
摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The method may also be useful for the physical separation and/or enrichment of two or more different or individual microorganism species contained in a mixed test sample. The invention further provides for spectroscopic interrogation of the separated microorganism sample(s) to produce measurements of the microorganism and characterizing and/or identifying the microorganism(s) in the sample using said spectroscopic measurements.
摘要翻译：本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。该方法还可用于混合测试样品中包含的两种或多种不同或单独的微生物物质的物理分离和/或富集。本发明进一步提供分离的微生物样品的光谱询问以产生微生物的测量，并使用所述光谱测量来表征和/或鉴定样品中的微生物。

6. 发明申请

US20130309714A1 Methods for Inactiviation And Extraction of Acid-Fast Bacteria for Characterization And/Or Identification Using Mass Spectrometry 有权
标题翻译：使用质谱法鉴定和/或鉴定酸快速细菌的灭活和提取方法
公开(公告)号：US20130309714A1
公开(公告)日：2013-11-21
申请号：US13828119
申请日：2013-03-14
申请人： Jones Hyman , Parampal Deol , Elizabeth Miller , Victoria Girard , Amber Gates , Sandrine Mailler , Maud Arsac , John Walsh
发明人： Jones Hyman , Parampal Deol , Elizabeth Miller , Victoria Girard , Amber Gates , Sandrine Mailler , Maud Arsac , John Walsh
IPC分类号： G01N1/40
CPC分类号： G01N1/4077 , C12Q1/24 , G01N33/5695 , G01N33/6851 , G01N2333/35 , G01N2333/355 , G01N2560/00
摘要： The present invention is directed to a method for inactivation and/or extraction of acid-fast bacteria (e.g., mycobacteria or nocardia), the method comprising the following sequential steps: (a) acquiring a test sample known to contain or that may contain acid-fast bacteria and suspending the test sample in a container containing ethanol and beads; (b) bead beating and/or vortexing the container to break up clumps and/or disrupt acid-fast bacteria cells in the container; and (c) subsequently incubating the suspension for at least about 3 minutes at room temperature to inactivate any acid-fast bacteria contained in the test sample. In accordance with the present invention, the test sample can subsequently be pelleted by centrifugation, resuspended with formic acid, acetonitrile added, and subjected to mass spectrometry for characterization and/or identification of the acid-fast bacteria.
摘要翻译：本发明涉及一种灭活和/或提取酸性抗菌（例如分枝杆菌或诺卡氏菌）的方法，该方法包括以下顺序步骤：（a）获得已知含有或可含有酸的测试样品将试样悬浮在含有乙醇和珠粒的容器中; （b）珠子打浆和/或涡旋容器以分解块和/或破坏容器中的耐酸细菌细胞; 和（c）随后在室温下将悬浮液温育至少约3分钟以灭活测试样品中所含的任何耐酸细菌。根据本发明，随后通过离心将测试样品沉淀，用甲酸重悬浮，加入乙腈，并进行质谱鉴定和/或鉴定耐酸细菌。

7. 发明申请

US20100136609A1 Method for separation, characterization and/or identification of microorganisms using raman spectroscopy 审中-公开
标题翻译：使用拉曼光谱法分离，表征和/或鉴定微生物的方法
公开(公告)号：US20100136609A1
公开(公告)日：2010-06-03
申请号：US12589976
申请日：2009-10-30
申请人： Bradford Clay , Jones Hyman , John Walsh , Thurman Thorpe , Christopher Ronsick
发明人： Bradford Clay , Jones Hyman , John Walsh , Thurman Thorpe , Christopher Ronsick
IPC分类号： C12Q1/04 , C12Q1/02
CPC分类号： C12Q1/04 , G01N21/35 , G01N21/3581 , G01N21/359 , G01N21/47 , G01N21/64 , G01N21/65 , G01N33/6848
摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for Raman spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said Raman spectroscopic measurements.
摘要翻译：本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。本发明进一步提供分离的微生物样品的拉曼光谱询问以产生微生物的测量，并使用所述拉曼光谱测量来表征和/或鉴定样品中的微生物。

8. 发明授权

US10415075B2 Method for separation, characterization and/or identification of microorganisms using mass spectrometry 有权
公开(公告)号：US10415075B2
公开(公告)日：2019-09-17
申请号：US12589936
申请日：2009-10-30
申请人： Jones Hyman , Bradford Clay , John Walsh , Thurman Thorpe
发明人： Jones Hyman , Bradford Clay , John Walsh , Thurman Thorpe
IPC分类号： C12Q1/04 , G01N33/68 , H01J49/00
摘要： A method is described to separate, characterize and/or identify microorganisms in a test sample. Said method comprises the steps of (i) lysing non-microorganism cells that might be present in the sample, (ii) separating the microorganisms as a pellet in the sample post-lysing non-microorganism cells, (iii) interrogating the pellet containing microorganisms by mass spectrometry to produce mass spectra of microorganisms, and (iv) characterizing and/or identifying microorganisms in the sample. The method may be used in characterizing/identifying microorganisms in complex samples, e.g., blood containing culture media.

9. 发明授权

US09175329B2 Methods for antimicrobial resistance determination 有权
标题翻译：耐药性测定方法
公开(公告)号：US09175329B2
公开(公告)日：2015-11-03
申请号：US14090169
申请日：2013-11-26
申请人： John Walsh , Jones Hyman
发明人： John Walsh , Jones Hyman
IPC分类号： C12Q1/14 , C12Q1/04 , C12Q1/02 , G01N33/569
CPC分类号： C12Q1/04 , C12Q1/025 , G01N33/56911
摘要： The present invention relates to methods and systems for determining the antibiotic-resistance status of microorganisms. The invention further provides methods for determining the antibiotic-resistance status of microorganisms in situ within a single system.
摘要翻译：本发明涉及用于测定微生物抗生素抗性状态的方法和系统。本发明还提供了在单个系统内测定原位微生物的抗生素抗性状态的方法。

10. 发明申请

US20140186881A1 Method and System for Detection and/or Characterization of a Biological Particle in a Sample 审中-公开
标题翻译：用于样品中生物颗粒检测和/或表征的方法和系统
公开(公告)号：US20140186881A1
公开(公告)日：2014-07-03
申请号：US14200662
申请日：2014-03-07
申请人： John Walsh , Jones Hyman , Bradford Clay , Thurman Thorpe
发明人： John Walsh , Jones Hyman , Bradford Clay , Thurman Thorpe
IPC分类号： C12Q1/04 , C12Q1/10
CPC分类号： C12Q1/04 , C12Q1/10 , G01N21/31 , G01N21/47 , G01N21/64 , G01N21/6486 , G01N2021/6419 , G01N2021/6421
摘要： The present invention provides a method and system for monitoring, detecting, and/or characterizing a biological particle that may be present in a sample whereby the method may be accomplished non-invasively by utilizing a time-dependent spectroscopic technique to obtain at least two measurements of a growth composition comprising a sample and correlating said measurements for the detection and/or characterization of a biological particle, that may be present in the sample.
摘要翻译：本发明提供了一种用于监测，检测和/或表征可能存在于样品中的生物颗粒的方法和系统，其中所述方法可以非侵入地通过利用时间依赖的光谱技术来获得至少两个测量的生长组合物，其包含样品并将所述测量结果与可能存在于样品中的生物颗粒的检测和/或表征相关联。

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