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1. 发明授权

US06770452B1 Methods for the identification of inhibitors of serine acetyltransferase activity in plants 失效
标题翻译：鉴定植物中丝氨酸乙酰转移酶活性抑制剂的方法
公开(公告)号：US06770452B1
公开(公告)日：2004-08-03
申请号：US10402818
申请日：2003-03-28
申请人： John W Rice , Lining Guo , Keith Davis , Adel Zayed , Robert Ascenzi , Joseph Cameron Mitchell , Daniel N Riggsbee , Douglas Boyes , Rao Mulpuri , Neil Hoffman , Susanne Kjemtrup , Carol Hamilton , Jeffrey Woessner , Jorn Gorlach
发明人： John W Rice , Lining Guo , Keith Davis , Adel Zayed , Robert Ascenzi , Joseph Cameron Mitchell , Daniel N Riggsbee , Douglas Boyes , Rao Mulpuri , Neil Hoffman , Susanne Kjemtrup , Carol Hamilton , Jeffrey Woessner , Jorn Gorlach
IPC分类号： C12Q148
CPC分类号： C12N9/1029 , C12N15/8261 , C12N15/8274 , C12Q1/48 , G01N2333/91057 , G01N2500/02 , Y02A40/146
摘要： The present inventors have discovered that serine acetyltransferase (SAT) is essential for plant growth. Specifically, the inhibition of SAT gene expression in plant seedlings results in reduced growth and altered pigmentation. Thus, SAT is useful as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of herbicides by measuring the activity of an SAT in the presence and absence of a compound, wherein an alteration of SAT activity in the presence of the compound indicates the compound as a candidate for a herbicide.
摘要翻译：本发明人发现丝氨酸乙酰转移酶（SAT）对于植物生长是必需的。具体地说，在植物幼苗中抑制SAT基因表达导致生长减少和色素沉着改变。因此，SAT可用作鉴定除草剂的靶标。因此，本发明提供了通过在化合物存在和不存在下测量SAT的活性来鉴定除草剂的方法，其中在化合物存在下SAT活性的改变表示作为除草剂候选物的化合物。

2. 发明申请

US20050112715A1 Methods for the identification of inhibitors of pectin esterase expression or activity in plants 审中-公开
标题翻译：用于鉴定植物中果胶酯酶表达或活性抑制剂的方法
公开(公告)号：US20050112715A1
公开(公告)日：2005-05-26
申请号：US10717879
申请日：2003-11-20
申请人： Adel Zayed , Robert Ascenzi , Douglas Boyes , Rao Mulpuri , Neil Hoffman , Keith Davis , Jorn Gorlach , Jeffrey Woessner , Carol Hamilton , Kenneth Phillips
发明人： Adel Zayed , Robert Ascenzi , Douglas Boyes , Rao Mulpuri , Neil Hoffman , Keith Davis , Jorn Gorlach , Jeffrey Woessner , Carol Hamilton , Kenneth Phillips
IPC分类号： C12Q1/44 , A01N25/00
CPC分类号： C12Q1/44 , G01N2500/00
摘要： The present inventors have discovered that pectin esterase is essential for plant growth. Specifically, the inhibition of pectin esterase gene expression resulted in seedlings that were damaged, short, and stunted at a very early growth stage. Thus, pectin esterase can be used as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of compounds that inhibit pectin esterase expression or activity, comprising: contacting a compound with a pectin esterase and detecting the presence and/or absence of binding between said compound and said pectin esterase, or detecting a decrease in pectin esterase expression or activity. The methods of the invention are useful for the identification of herbicides.
摘要翻译：本发明人发现，果胶酯酶对植物生长至关重要。具体来说，果胶酯酶基因表达的抑制导致幼苗在非常早期的生长阶段受损，短缺和发育迟缓。因此，果胶酯酶可以用作鉴定除草剂的靶标。因此，本发明提供了鉴定抑制果胶酯酶表达或活性的化合物的方法，包括：使化合物与果胶酯酶接触并检测所述化合物与所述果胶酯酶之间的结合的存在和/或不存在，或检测果胶酯酶表达或活性降低。本发明的方法可用于鉴定除草剂。

3. 发明申请

US20050130118A1 Methods for identifying inhibitors of chlorophyll synthase 审中-公开
标题翻译：鉴定叶绿素合酶抑制剂的方法
公开(公告)号：US20050130118A1
公开(公告)日：2005-06-16
申请号：US10737014
申请日：2003-12-16
申请人： Abubakr Aslamkhan , Lining Guo , Rao Mulpuri , Neil Hoffman , Susanne Kjemtrup , Jocelyn Kearney , Cory Christensen , Keith Davis , Adel Zayed , Robert Ascenzi , Douglas Boyes
发明人： Abubakr Aslamkhan , Lining Guo , Rao Mulpuri , Neil Hoffman , Susanne Kjemtrup , Jocelyn Kearney , Cory Christensen , Keith Davis , Adel Zayed , Robert Ascenzi , Douglas Boyes
IPC分类号： C12Q1/00 , G01N33/573
CPC分类号： G01N33/573
摘要： The present inventors have discovered that antisense suppression of a chlorophyll synthase (CS) gene results in plants exhibiting one or more of chlorosis, reduced growth, and altered development. Thus, the present inventors have discovered that the protein encoded by the CS gene is essential for normal plant growth and development, and is useful as a target for the identification of compounds as antibiotics and herbicides, especially herbicides. The present invention is directed to methods for identifying inhibitors of a CS enzyme by incubating a CS polypeptide with a chlorophyllide and a phopholipid substrate in the presence and absence of a test compound under conditions suitable for the CS enzyme activity, adding a solution to the incubation reactions comprising a water immiscible organic solvent, a water-soluble alcohol, and a water-soluble dye that absorbs in the range of one or both the excitation and emission wavelength ranges of the chlorophyllide substrate, and measuring the fluorescence of the incubation reactions at from about 650 to 750 nm, using from about 425 to 445 nm as excitation wavelength, wherein a decrease in the fluorescence in the presence of the test compound indicates that the compound is a CS inhibitor.
摘要翻译：本发明人已经发现，叶绿素合酶（CS）基因的反义抑制导致植物表现出一种或多种褪绿，生长减少和改变发育。因此，本发明人已经发现，由CS基因编码的蛋白质对于正常的植物生长和发育是必需的，并且可用作鉴定作为抗生素和除草剂，特别是除草剂的化合物的靶标。本发明涉及通过在适合于CS酶活性的条件下，在存在和不存在测试化合物的情况下，将CS多肽与叶绿素和磷脂底物一起温育来鉴定CS酶抑制剂的方法，向培养基中加入溶液包括与水不溶混的有机溶剂，水溶性醇和在所述叶绿素底物的激发和发射波长范围中的一个或两个的范围内吸收的水溶性染料的反应，以及测量从约650至750nm，使用约425至445nm作为激发波长，其中在测试化合物存在下荧光的降低表明该化合物是CS抑制剂。

4. 发明申请

US20050100879A1 Methods for the identification of inhibitors of flavanone 3-hydroxylase expression or activity in plants 审中-公开
标题翻译：鉴定黄烷酮3-羟化酶抑制剂在植物中的表达或活性的方法
公开(公告)号：US20050100879A1
公开(公告)日：2005-05-12
申请号：US10702306
申请日：2003-11-06
申请人： Keith Davis , Adel Zayed , Robert Ascenzi , Douglas Boyes , Neil Hoffman , Jorn Gorlach , Jeffrey Woessner , Carol Hamilton , Kenneth Phillips
发明人： Keith Davis , Adel Zayed , Robert Ascenzi , Douglas Boyes , Neil Hoffman , Jorn Gorlach , Jeffrey Woessner , Carol Hamilton , Kenneth Phillips
IPC分类号： A01N25/00 , C12Q1/00 , C12Q1/26 , C12Q1/68 , G01N33/50
CPC分类号： G01N33/5097 , C12Q1/26 , G01N2430/20 , G01N2500/00
摘要： The present inventors have discovered that Flavanone 3-hydroxylase (“FHT”) is essential for plant growth. Specifically, the inhibition of FHT gene expression in plant seedlings results in small and chlorotic seedlings. Thus, FHT can be used as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of compounds that inhibit FHT expression or activity. The present invention can be used to identify compounds having herbicide activity by contacting a compound with FHT and detecting the presence and/or absence of binding between the compound and the FHT, or alternatively, detecting a decrease in FHT expression or activity.
摘要翻译：本发明人已经发现，黄酮酮3-羟化酶（“FHT”）对于植物生长是必需的。具体来说，植物幼苗中FHT基因表达的抑制作用会导致幼苗和幼苗的生长。因此，FHT可用作鉴定除草剂的靶标。因此，本发明提供了鉴定抑制FHT表达或活性的化合物的方法。本发明可用于通过使化合物与FHT接触并检测化合物与FHT之间的结合的存在和/或不存在，或者检测FHT表达或活性的降低来鉴定具有除草剂活性的化合物。

5. 发明申请

US20050101485A1 Methods for the identification of inhibitors of biotin synthase expression or activity in plants 审中-公开
标题翻译：用于鉴定植物中生物素合酶表达抑制剂或活性的方法
公开(公告)号：US20050101485A1
公开(公告)日：2005-05-12
申请号：US10702364
申请日：2003-11-06
申请人： Neil Hoffman , Jorn Gorlach , Douglas Boyes , Adel Zayed , Jeffrey Woessner , Carol Hamilton , Keith Davis , Robert Ascenzi
发明人： Neil Hoffman , Jorn Gorlach , Douglas Boyes , Adel Zayed , Jeffrey Woessner , Carol Hamilton , Keith Davis , Robert Ascenzi
IPC分类号： A01N61/00 , C12Q1/48 , G01N33/50 , G01N33/573 , A01N25/00 , C12Q1/37
CPC分类号： G01N33/5097 , A01N61/00 , C12Q1/48 , G01N33/573 , G01N2500/02 , G01N2500/04
摘要： The present inventors have discovered that Biotin synthase (BS) is essential for plant growth. Specifically, inhibition of BS gene expression in plant seedlings results in severe chlorosis and reduced growth. Thus, BS can be used as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of compounds that inhibit BS expression or activity, and as such, the methods of the invention are useful for the identification of herbicides.
摘要翻译：本发明人已经发现，生物素合酶（BS）对于植物生长是必需的。具体来说，植物幼苗中BS基因表达的抑制导致严重的萎黄症和生长减少。因此，BS可以用作鉴定除草剂的目标。因此，本发明提供了鉴定抑制BS表达或活性的化合物的方法，因此，本发明的方法可用于鉴定除草剂。

6. 发明申请

US20120277117A1 HYDROPONIC APPARATUS AND METHODS OF USE 审中-公开
标题翻译：水力装置及其使用方法
公开(公告)号：US20120277117A1
公开(公告)日：2012-11-01
申请号：US13203516
申请日：2010-02-26
申请人： Adel Zayed , Michael L. Jones , Chris Tierney , Matthew Miller
发明人： Adel Zayed , Michael L. Jones , Chris Tierney , Matthew Miller
IPC分类号： C40B60/12 , C40B30/06
CPC分类号： A01G7/00 , A01G31/02 , Y02P60/216
摘要： Hydroponic apparatus and methods for the high-throughput screening plants are disclosed. In one aspect, a method for the high-throughput screening of plants is disclosed. The method comprises germinating a plurality of plants in a hydroponic apparatus; selecting one or more plants having substantially uniform qualities from the plurality of germinated plants to form a population of plants; growing the population of selected plants in a controlled environment; and screening one or more plants in the population at least once during a growing period to determine the presence or absence of one or more predetermined characteristics
摘要翻译：公开了用于高通量筛选装置的水培装置和方法。一方面，公开了一种用于植物高通量筛选的方法。该方法包括在水培装置中发芽多个植物; 从所述多个发芽植物中选择具有基本均匀质量的一种或多种植物以形成植物群; 在受控环境中种植选定植物的种群; 以及在生长期间至少一次筛选所述群体中的一种或多种植物以确定是否存在一种或多种预定特征

7. 发明授权

US06800459B2 Methods and compositions for the modulation of chorismate synthase and chorismate mutase expression or activity in plants 失效
标题翻译：调节草酸合酶和草酸转化酶表达或活性的方法和组合物
公开(公告)号：US06800459B2
公开(公告)日：2004-10-05
申请号：US10267763
申请日：2002-10-09
申请人： Douglas C. Boyes , Keith R. Davis , Jeffrey P. Woessner , Jörn Görlach , Carol M. Hamilton , Neil E. Hoffman , Andreas S. Klöti , Adel Zayed , Robert A. Ascenzi
发明人： Douglas C. Boyes , Keith R. Davis , Jeffrey P. Woessner , Jörn Görlach , Carol M. Hamilton , Neil E. Hoffman , Andreas S. Klöti , Adel Zayed , Robert A. Ascenzi
IPC分类号： C12N2106
CPC分类号： G01N33/68 , G01N2500/00
摘要： The present inventors have discovered that chorismate mutase and chorismate synthase are essential for plant growth. Specifically, the inhibition of chorismate mutase or chorismate synthase gene expression in plant seedlings results in severe chlorosis, reduced growth and developmental abnormalities. The inventors have proven that chorismate synthase and chorismate mutase can be used as targets for the identification of herbicides. Thus, the invention provides methods for the identification of chemicals that modulate chorismate synthase and chorismate mutase biochemical reactions. The methods of the invention are useful for the identification of herbicides and for the inhibition of plant growth and development.
摘要翻译：本发明人已经发现，草酸修饰酶和绒毛膜合成酶对于植物生长是必需的。具体来说，植物幼苗中的chorismate mutase或chorismate synthase gene表达的抑制导致严重的萎黄症，生长发育减退和发育异常。本发明人已经证明，可以使用草酸合成酶和氯酸盐变位酶作为鉴定除草剂的靶标。因此，本发明提供了用于鉴定调节绒毛膜合成酶和氯酸盐变位酶生化反应的化学物质的方法。本发明的方法可用于鉴定除草剂和用于抑制植物的生长和发育。

8. 发明授权

US06465217B1 Methods and compositions for the modulation of chorismate synthase and chorismate mutase expression or activity in plants 失效
标题翻译：调节草酸合酶和草酸转化酶表达或活性的方法和组合物
公开(公告)号：US06465217B1
公开(公告)日：2002-10-15
申请号：US09610040
申请日：2000-07-05
申请人： Douglas C. Boyes , Keith R. Davis , Jeffrey P. Woessner , Jörn Görlach , Carol M. Hamilton , Neil E. Hoffman , Andreas S. Klöti , Adel Zayed , Robert A. Ascenzi
发明人： Douglas C. Boyes , Keith R. Davis , Jeffrey P. Woessner , Jörn Görlach , Carol M. Hamilton , Neil E. Hoffman , Andreas S. Klöti , Adel Zayed , Robert A. Ascenzi
IPC分类号： C12N2106
CPC分类号： G01N33/68 , G01N2500/00
摘要： The present inventors have discovered that chorismate mutase and chorismate synthase are essential for plant growth. Specifically, the inhibition of chorismate mutase or chorismate synthase gene expression in plant seedlings results in severe chlorosis, reduced growth and developmental abnormalities. Thus, in one aspect the invention provides compositions for the modulation of plant growth or development comprising chorismate synthase and chorismate mutase antisense and sense polynucleotides, dsRNA and ribozymes, and related expression cassettes and vectors. The compositions of the invention are particularly useful for the modulation and inhibition of plant growth. The invention further provides plants, plant cells, and seeds containing the polynucleotides of the invention. The inventors have proven that chorismate synthase and chorismate mutase can be used as targets for the identification of herbicides. Thus, the invention provides methods for the identification of chemicals that modulate chorismate synthase and chorismate mutase biochemical reactions. The methods of the invention are useful for the identification of herbicides and for the inhibition of plant growth and development. In addition, the methods of the invention are useful for the identification of compounds that stimulate the expression or function of chorismate synthase or chorismate mutase expression or function. Such compounds can be used to promote plant growth and development.
摘要翻译：本发明人已经发现，草酸修饰酶和绒毛膜合成酶对于植物生长是必需的。具体来说，植物幼苗中的chorismate mutase或chorismate synthase gene表达的抑制导致严重的萎黄症，生长发育减退和发育异常。因此，在一个方面，本发明提供了用于调节植物生长或发育的组合物，其包含绒毛膜合成酶和辅助组氨酸变位酶反义和有义多核苷酸，dsRNA和核酶以及相关的表达盒和载体。本发明的组合物特别可用于调节和抑制植物生长。本发明还提供含有本发明的多核苷酸的植物，植物细胞和种子。本发明人已经证明，可以使用草酸合成酶和氯酸盐变位酶作为鉴定除草剂的靶标。因此，本发明提供了用于鉴定调节绒毛膜合成酶和氯酸盐变位酶生化反应的化学物质的方法。本发明的方法可用于鉴定除草剂和用于抑制植物的生长和发育。此外，本发明的方法可用于鉴定刺激基底膜合成酶或调节酸变位酶表达或功能的表达或功能的化合物。这些化合物可用于促进植物的生长和发育。

9. 发明授权

US06582900B1 Methods for the identification of modulators of magnesium chelatase expression or activity in plants 失效
标题翻译：鉴定镁螯合酶表达或植物活性调节剂的方法
公开(公告)号：US06582900B1
公开(公告)日：2003-06-24
申请号：US09659310
申请日：2000-09-12
申请人： Jeffrey P. Woessner , Adel Zayed , Jörn Görlach , Douglas C. Boyes , Keith R. Davis , Carol M. Hamilton , Neil E. Hoffman , Andreas S. Kloti , Robert A. Ascenzi
发明人： Jeffrey P. Woessner , Adel Zayed , Jörn Görlach , Douglas C. Boyes , Keith R. Davis , Carol M. Hamilton , Neil E. Hoffman , Andreas S. Kloti , Robert A. Ascenzi
IPC分类号： C12Q100
CPC分类号： C12Q1/25 , A01N61/00 , G01N2500/00
摘要： The present inventors have discovered that Mg-chelatase is essential for the growth of Arabidopsis. Specifically, the inhibition of Mg-chelatase CHL H gene expression in Arabidopsis seedlings results in varying levels of chlorosis (yellowing), significantly reduced growth and developmental abnormalities. Thus, Arabidopsis Mg-chelatase can be used as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of compounds that modulate Arabidopsis Mg-chelatase expression or activity, comprising: contacting a compound with a Arabidopsis Mg-chelatase, or a subunit thereof, and detecting the presence and/or absence of binding between said compound and said Mg-chelatase, or detecting a change in Mg-chelatase expression or activity. The methods of the invention are useful for the identification of herbicides and other compounds that can modulate plant growth and development. In addition, the methods of the invention are useful for the identification of compounds that stimulate the expression or function of Mg-chelatase expression or function. Such compounds can be used to promote or manipulate plant growth and development.
摘要翻译：本发明人已经发现，Mg螯合酶对于拟南芥的生长是必需的。具体来说，拟南芥幼苗中Mg-螯合酶CHL H基因表达的抑制导致不同程度的褪绿（黄化），显着降低生长和发育异常。因此，拟南芥Mg螯合酶可用作鉴定除草剂的靶标。因此，本发明提供了鉴定调节拟南芥Mg-螯合酶表达或活性的化合物的方法，其包括：使化合物与拟南芥Mg-螯合酶或其亚单位接触，并检测其中是否存在结合所述化合物和所述Mg-螯合酶，或检测Mg螯合酶表达或活性的变化。本发明的方法可用于鉴定可以调节植物生长和发育的除草剂和其它化合物。此外，本发明的方法可用于鉴定刺激Mg-螯合酶表达或功能的表达或功能的化合物。这些化合物可用于促进或操纵植物的生长和发育。

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