专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06582900B1 Methods for the identification of modulators of magnesium chelatase expression or activity in plants 失效
标题翻译：鉴定镁螯合酶表达或植物活性调节剂的方法
公开(公告)号：US06582900B1
公开(公告)日：2003-06-24
申请号：US09659310
申请日：2000-09-12
申请人： Jeffrey P. Woessner , Adel Zayed , Jörn Görlach , Douglas C. Boyes , Keith R. Davis , Carol M. Hamilton , Neil E. Hoffman , Andreas S. Kloti , Robert A. Ascenzi
发明人： Jeffrey P. Woessner , Adel Zayed , Jörn Görlach , Douglas C. Boyes , Keith R. Davis , Carol M. Hamilton , Neil E. Hoffman , Andreas S. Kloti , Robert A. Ascenzi
IPC分类号： C12Q100
CPC分类号： C12Q1/25 , A01N61/00 , G01N2500/00
摘要： The present inventors have discovered that Mg-chelatase is essential for the growth of Arabidopsis. Specifically, the inhibition of Mg-chelatase CHL H gene expression in Arabidopsis seedlings results in varying levels of chlorosis (yellowing), significantly reduced growth and developmental abnormalities. Thus, Arabidopsis Mg-chelatase can be used as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of compounds that modulate Arabidopsis Mg-chelatase expression or activity, comprising: contacting a compound with a Arabidopsis Mg-chelatase, or a subunit thereof, and detecting the presence and/or absence of binding between said compound and said Mg-chelatase, or detecting a change in Mg-chelatase expression or activity. The methods of the invention are useful for the identification of herbicides and other compounds that can modulate plant growth and development. In addition, the methods of the invention are useful for the identification of compounds that stimulate the expression or function of Mg-chelatase expression or function. Such compounds can be used to promote or manipulate plant growth and development.
摘要翻译：本发明人已经发现，Mg螯合酶对于拟南芥的生长是必需的。具体来说，拟南芥幼苗中Mg-螯合酶CHL H基因表达的抑制导致不同程度的褪绿（黄化），显着降低生长和发育异常。因此，拟南芥Mg螯合酶可用作鉴定除草剂的靶标。因此，本发明提供了鉴定调节拟南芥Mg-螯合酶表达或活性的化合物的方法，其包括：使化合物与拟南芥Mg-螯合酶或其亚单位接触，并检测其中是否存在结合所述化合物和所述Mg-螯合酶，或检测Mg螯合酶表达或活性的变化。本发明的方法可用于鉴定可以调节植物生长和发育的除草剂和其它化合物。此外，本发明的方法可用于鉴定刺激Mg-螯合酶表达或功能的表达或功能的化合物。这些化合物可用于促进或操纵植物的生长和发育。

2. 发明授权

US06800459B2 Methods and compositions for the modulation of chorismate synthase and chorismate mutase expression or activity in plants 失效
标题翻译：调节草酸合酶和草酸转化酶表达或活性的方法和组合物
公开(公告)号：US06800459B2
公开(公告)日：2004-10-05
申请号：US10267763
申请日：2002-10-09
申请人： Douglas C. Boyes , Keith R. Davis , Jeffrey P. Woessner , Jörn Görlach , Carol M. Hamilton , Neil E. Hoffman , Andreas S. Klöti , Adel Zayed , Robert A. Ascenzi
发明人： Douglas C. Boyes , Keith R. Davis , Jeffrey P. Woessner , Jörn Görlach , Carol M. Hamilton , Neil E. Hoffman , Andreas S. Klöti , Adel Zayed , Robert A. Ascenzi
IPC分类号： C12N2106
CPC分类号： G01N33/68 , G01N2500/00
摘要： The present inventors have discovered that chorismate mutase and chorismate synthase are essential for plant growth. Specifically, the inhibition of chorismate mutase or chorismate synthase gene expression in plant seedlings results in severe chlorosis, reduced growth and developmental abnormalities. The inventors have proven that chorismate synthase and chorismate mutase can be used as targets for the identification of herbicides. Thus, the invention provides methods for the identification of chemicals that modulate chorismate synthase and chorismate mutase biochemical reactions. The methods of the invention are useful for the identification of herbicides and for the inhibition of plant growth and development.
摘要翻译：本发明人已经发现，草酸修饰酶和绒毛膜合成酶对于植物生长是必需的。具体来说，植物幼苗中的chorismate mutase或chorismate synthase gene表达的抑制导致严重的萎黄症，生长发育减退和发育异常。本发明人已经证明，可以使用草酸合成酶和氯酸盐变位酶作为鉴定除草剂的靶标。因此，本发明提供了用于鉴定调节绒毛膜合成酶和氯酸盐变位酶生化反应的化学物质的方法。本发明的方法可用于鉴定除草剂和用于抑制植物的生长和发育。

3. 发明授权

US06465217B1 Methods and compositions for the modulation of chorismate synthase and chorismate mutase expression or activity in plants 失效
标题翻译：调节草酸合酶和草酸转化酶表达或活性的方法和组合物
公开(公告)号：US06465217B1
公开(公告)日：2002-10-15
申请号：US09610040
申请日：2000-07-05
申请人： Douglas C. Boyes , Keith R. Davis , Jeffrey P. Woessner , Jörn Görlach , Carol M. Hamilton , Neil E. Hoffman , Andreas S. Klöti , Adel Zayed , Robert A. Ascenzi
发明人： Douglas C. Boyes , Keith R. Davis , Jeffrey P. Woessner , Jörn Görlach , Carol M. Hamilton , Neil E. Hoffman , Andreas S. Klöti , Adel Zayed , Robert A. Ascenzi
IPC分类号： C12N2106
CPC分类号： G01N33/68 , G01N2500/00
摘要： The present inventors have discovered that chorismate mutase and chorismate synthase are essential for plant growth. Specifically, the inhibition of chorismate mutase or chorismate synthase gene expression in plant seedlings results in severe chlorosis, reduced growth and developmental abnormalities. Thus, in one aspect the invention provides compositions for the modulation of plant growth or development comprising chorismate synthase and chorismate mutase antisense and sense polynucleotides, dsRNA and ribozymes, and related expression cassettes and vectors. The compositions of the invention are particularly useful for the modulation and inhibition of plant growth. The invention further provides plants, plant cells, and seeds containing the polynucleotides of the invention. The inventors have proven that chorismate synthase and chorismate mutase can be used as targets for the identification of herbicides. Thus, the invention provides methods for the identification of chemicals that modulate chorismate synthase and chorismate mutase biochemical reactions. The methods of the invention are useful for the identification of herbicides and for the inhibition of plant growth and development. In addition, the methods of the invention are useful for the identification of compounds that stimulate the expression or function of chorismate synthase or chorismate mutase expression or function. Such compounds can be used to promote plant growth and development.
摘要翻译：本发明人已经发现，草酸修饰酶和绒毛膜合成酶对于植物生长是必需的。具体来说，植物幼苗中的chorismate mutase或chorismate synthase gene表达的抑制导致严重的萎黄症，生长发育减退和发育异常。因此，在一个方面，本发明提供了用于调节植物生长或发育的组合物，其包含绒毛膜合成酶和辅助组氨酸变位酶反义和有义多核苷酸，dsRNA和核酶以及相关的表达盒和载体。本发明的组合物特别可用于调节和抑制植物生长。本发明还提供含有本发明的多核苷酸的植物，植物细胞和种子。本发明人已经证明，可以使用草酸合成酶和氯酸盐变位酶作为鉴定除草剂的靶标。因此，本发明提供了用于鉴定调节绒毛膜合成酶和氯酸盐变位酶生化反应的化学物质的方法。本发明的方法可用于鉴定除草剂和用于抑制植物的生长和发育。此外，本发明的方法可用于鉴定刺激基底膜合成酶或调节酸变位酶表达或功能的表达或功能的化合物。这些化合物可用于促进植物的生长和发育。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式