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1. 发明授权

US6143524A Peptide and protein fusions to thioredoxin, thioredoxin-like molecules, and modified thioredoxin-like molecules 失效
标题翻译：肽和蛋白质融合到硫氧还蛋白，硫氧还蛋白样分子和改良的硫氧还蛋白样分子
公开(公告)号：US6143524A
公开(公告)日：2000-11-07
申请号：US810436
申请日：1997-03-04
申请人： John McCoy , Elizabeth DiBlasio-Smith , Kathleen Grant , Edward R. LaVallie
发明人： John McCoy , Elizabeth DiBlasio-Smith , Kathleen Grant , Edward R. LaVallie
IPC分类号： C07K14/00 , C07K14/475 , C07K14/51 , C07K14/52 , C07K14/53 , C07K14/54 , C07K14/55 , C12N1/06 , C12N9/02 , C12N15/24 , C12N15/62 , C12N15/63
CPC分类号： C12N9/0036 , C07K14/00 , C07K14/475 , C07K14/51 , C07K14/52 , C07K14/523 , C07K14/53 , C07K14/54 , C07K14/5403 , C07K14/5406 , C07K14/5409 , C07K14/5412 , C07K14/5431 , C07K14/55 , C12N1/066 , C12N15/62 , C12Y108/01008 , C07K2319/00 , C07K2319/02 , C07K2319/20 , C07K2319/35 , C07K2319/75
摘要： Provided is a fusion molecule comprising a DNA sequence encoding a thioredoxin-like protein fused to a DNA sequence encoding a second peptide or protein. The peptide or protein may be fused to the amino terminus of the thioredoxin-like molecule, the carboxyl terminus of the thioredoxin-like molecule, or within the thioredoxin-like molecule, for example at the active-site loop of the molecule. The fusion molecule may be modified to introduce one or more metal-binding/chelating amino-acid residues to aid in purification. Expression of this fusion molecule under the control of a regulatory sequence capable of directing its expression in a desired host cell, produces high levels of stable and soluble fusion protein. The fusion protein, located in the bacterial cytoplasm, may be selectively released from the cell by osmotic shock or freeze/thaw procedures. It may be optionally cleaved to liberate the soluble, correctly folded heterologous protein from the thioredoxin-like portion.
摘要翻译：提供了包含编码与编码第二肽或蛋白质的DNA序列融合的硫氧还蛋白样蛋白质的DNA序列的融合分子。肽或蛋白质可以与硫氧还蛋白样分子的氨基末端，硫氧还蛋白样分子的羧基末端或硫氧还蛋白样分子内的融合，例如在分子的活性位点环上。可以修饰融合分子以引入一种或多种金属结合/螯合氨基酸残基以有助于纯化。在能够将其表达在所需宿主细胞中的调节序列控制下的该融合分子的表达产生高水平的稳定和可溶性融合蛋白。位于细菌细胞质中的融合蛋白可通过渗透休克或冷冻/解冻程序从细胞中选择性释放。可以任选地切割以从硫氧还蛋白样部分释放可溶性的，正确折叠的异源蛋白质。

2. 发明授权

US5646016A Peptide and protein fusions to thioredoxin, thioredoxin-like molecules, and modified thioredoxin-like molecules 失效
标题翻译：肽和蛋白质融合到硫氧还蛋白，硫氧还蛋白样分子和改良的硫氧还蛋白样分子
公开(公告)号：US5646016A
公开(公告)日：1997-07-08
申请号：US165301
申请日：1993-12-10
申请人： John McCoy , Elizabeth DiBlasio-Smith , Kathleen Grant , Edward R. LaVallie
发明人： John McCoy , Elizabeth DiBlasio-Smith , Kathleen Grant , Edward R. LaVallie
IPC分类号： C07K14/00 , C07K14/475 , C07K14/51 , C07K14/52 , C07K14/53 , C07K14/54 , C07K14/55 , C12N1/06 , C12N9/02 , C12N15/24 , C12N15/62 , C07K1/22 , C12N1/00 , C12N15/63
CPC分类号： C12N9/0036 , C07K14/00 , C07K14/475 , C07K14/51 , C07K14/52 , C07K14/523 , C07K14/53 , C07K14/54 , C07K14/5403 , C07K14/5406 , C07K14/5409 , C07K14/5412 , C07K14/5431 , C07K14/55 , C12N1/066 , C12N15/62 , C12Y108/01008 , C07K2319/00 , C07K2319/02 , C07K2319/20 , C07K2319/35 , C07K2319/75
摘要： Provided is a fusion molecule comprising a DNA sequence encoding a thioredoxin-like protein fused to a DNA sequence encoding a second peptide or protein. The peptide or protein may be fused to the amino terminus of the thioredoxin-like molecule, the carboxyl terminus of the thioredoxin-like molecule, or within the thioredoxin-like molecule, for example at the active-site loop of said molecule. The fusion molecule may be modified to introduce one or more metal-binding/chelating amino-acid residues to aid in purification. Expression of this fusion molecule under the control of a regulatory sequence capable of directing its expression in a desired host cell, produces high levels of stable and soluble fusion protein. The fusion protein, located in the bacterial cytoplasm, may be selectively released from the cell by osmotic shock or freeze/thaw procedures. It may be optionally cleaved to liberate the soluble, correctly folded heterologous protein from the thioredoxin-like portion.
摘要翻译：提供了包含编码与编码第二肽或蛋白质的DNA序列融合的硫氧还蛋白样蛋白质的DNA序列的融合分子。肽或蛋白质可以融合到硫氧还蛋白样分子的氨基末端，硫氧还蛋白样分子的羧基末端，或硫氧还蛋白样分子内，例如在所述分子的活性位点环上。可以修饰融合分子以引入一种或多种金属结合/螯合氨基酸残基以有助于纯化。在能够将其表达在所需宿主细胞中的调节序列控制下的该融合分子的表达产生高水平的稳定和可溶性融合蛋白。位于细菌细胞质中的融合蛋白可通过渗透休克或冷冻/解冻程序从细胞中选择性释放。可以任选地切割以从硫氧还蛋白样部分释放可溶性的，正确折叠的异源蛋白质。

3. 发明授权

US5292646A Peptide and protein fusions to thioredoxin and thioredoxin-like molecules 失效
标题翻译：肽和蛋白质融合硫氧还蛋白和硫氧还蛋白样分子
公开(公告)号：US5292646A
公开(公告)日：1994-03-08
申请号：US921848
申请日：1992-07-28
申请人： John McCoy , Edward R. LaVallie
发明人： John McCoy , Edward R. LaVallie
IPC分类号： C07K14/00 , C07K14/475 , C07K14/51 , C07K14/52 , C07K14/53 , C07K14/54 , C07K14/55 , C12N1/06 , C12N9/02 , C12N15/62 , C12N1/00 , C07K13/00 , C12N5/10 , C12N15/63
CPC分类号： C12N9/0036 , C07K14/00 , C07K14/475 , C07K14/51 , C07K14/52 , C07K14/523 , C07K14/53 , C07K14/54 , C07K14/5403 , C07K14/5406 , C07K14/5409 , C07K14/5412 , C07K14/5431 , C07K14/55 , C12N1/066 , C12N15/62 , C12Y108/01008 , C07K2319/00 , C07K2319/02 , C07K2319/20 , C07K2319/35 , C07K2319/75
摘要： This invention provides a fusion molecule comprising a DNA sequence encoding a thioredoxin-like protein fused to the DNA sequence encoding a selected heterologous peptide or protein. The peptide or protein may be fused to the amino terminus of the thioredoxin-like molecule, the carboxyl terminus of the thioredoxin-like molecule, or within the thioredoxin-like molecule, for example at the active-site loop of said molecule. Expression of this fusion molecule under the control of a regulatory sequence capable of directing its expression in a desired host cell, produces high levels of stable and soluble fusion protein. The fusion protein, located in the bacterial cytoplasm, may be selectively released from the cell by osmotic shock or freeze/thaw procedures. It may be optionally cleaved to liberate the soluble, correctly folded heterologous protein from the thioredoxin-like portion.
摘要翻译：本发明提供一种融合分子，其包含编码与编码选择的异源肽或蛋白质的DNA序列融合的硫氧还蛋白样蛋白质的DNA序列。肽或蛋白质可以融合到硫氧还蛋白样分子的氨基末端，硫氧还蛋白样分子的羧基末端，或硫氧还蛋白样分子内，例如在所述分子的活性位点环上。在能够将其表达在所需宿主细胞中的调节序列控制下的该融合分子的表达产生高水平的稳定和可溶性融合蛋白。位于细菌细胞质中的融合蛋白可通过渗透休克或冷冻/解冻程序从细胞中选择性释放。可以任选地切割以从硫氧还蛋白样部分释放可溶性的，正确折叠的异源蛋白质。

4. 发明授权

US5270181A Peptide and protein fusions to thioredoxin and thioredoxin-like molecules 失效
标题翻译：肽和蛋白质融合硫氧还蛋白和硫氧还蛋白样分子
公开(公告)号：US5270181A
公开(公告)日：1993-12-14
申请号：US745382
申请日：1991-08-14
申请人： John McCoy , Edward R. LaVallie
发明人： John McCoy , Edward R. LaVallie
IPC分类号： A61K38/00 , C07K14/00 , C07K14/195 , C07K14/245 , C07K14/41 , C07K14/47 , C07K14/51 , C07K14/52 , C07K14/53 , C07K14/54 , C07K19/00 , C12N1/21 , C12N15/09 , C12N15/53 , C12N15/62 , C12N15/70 , C12N15/74 , C12P21/02 , C12R1/19 , C12N15/11 , C12N15/24
CPC分类号： C12N15/62 , C07K14/00 , C07K14/51 , C07K14/52 , C07K14/53 , C07K14/5403 , C07K14/5412 , C07K14/5431 , C07K2319/00 , C07K2319/02 , C07K2319/35 , C07K2319/75
摘要： This invention provides a fusion molecule comprising a DNA sequence encoding a thioredoxin-like protein fused to the DNA sequence encoding a selected heterologous peptide or protein. The peptide or protein may be fused to the amino terminus of the thioredoxin-like molecule, the carboxyl terminus of the thioredoxin-like molecule, or within the thioredoxin-like molecule, for example at the active-site loop of said molecule. Expression of this fusion molecule under the control of a regulatory sequence capable of directing its expression in a desired host cell, produces high levels of stable and soluble fusion protein. The fusion protein, located in the bacterial cytoplasm, may be selectively released from the cell by osmotic shock or freeze/thaw procedures. It may be optionally cleaved to liberate the soluble, correctly folded heterologous protein from the thioredoxin-like portion.
摘要翻译：本发明提供一种融合分子，其包含编码与编码选择的异源肽或蛋白质的DNA序列融合的硫氧还蛋白样蛋白质的DNA序列。肽或蛋白质可以融合到硫氧还蛋白样分子的氨基末端，硫氧还蛋白样分子的羧基末端，或硫氧还蛋白样分子内，例如在所述分子的活性位点环上。在能够将其表达在所需宿主细胞中的调节序列控制下的该融合分子的表达产生高水平的稳定和可溶性融合蛋白。位于细菌细胞质中的融合蛋白可通过渗透休克或冷冻/解冻程序从细胞中选择性释放。可以任选地切割以从硫氧还蛋白样部分释放可溶性的，正确折叠的异源蛋白质。

5. 发明申请

US20200350482A1 Shock resistant, efficient, low power radioisotope thermoelectroc generator 审中-公开
公开(公告)号：US20200350482A1
公开(公告)日：2020-11-05
申请号：US16501588
申请日：2019-05-02
申请人： Kavon Kazemzadah , John McCoy , Frederick Leavitt , Carmen Brown , Xiao Bing
发明人： Kavon Kazemzadah , John McCoy , Frederick Leavitt , Carmen Brown , Xiao Bing
IPC分类号： H01L35/32 , H01L35/30 , H01L35/16 , H01L35/04 , G21H1/04 , G21H1/06
摘要： This invention brings three new elements to the design of a low-power RTG; 1) an RHU suspension system, 2) a module tensioning system, and 3) a flexible thermal conductor. Taken together, these three elements enable the design of a system which finds optimal balancing of shock resistance with energy conversion efficiency.

6. 发明授权

US08419409B2 Molding station with deformable mold and method 有权
标题翻译：具有可变形模具和方法的成型站
公开(公告)号：US08419409B2
公开(公告)日：2013-04-16
申请号：US12519599
申请日：2007-12-20
申请人： Éric Milot , John McCoy
发明人： Éric Milot , John McCoy
IPC分类号： B29C43/02 , B29C43/34
CPC分类号： B28B7/06 , B28B13/0215 , B28B13/0295
摘要： A molding station and method comprising a hopper having a mouth closable with at least a lower door and a mold to be filled, the hopper and the mold being movable to a filling position in which the hopper is closed by the lower door; the lower door then opening to allow an amount of moldable material to be released into the mold; the mold being made in a deformable material, so that an interior volume of the mold is temporarily reduced when releasing the moldable material into the mold.
摘要翻译：一种成型台和方法，包括具有可关闭至少下门的料斗和待填充的模具的料斗，料斗和模具可移动到料斗由下门关闭的填充位置; 下门然后打开以允许一定量的可模制材料被释放到模具中; 模具制成可变形材料，使得当将可模制材料释放到模具中时，模具的内部容积暂时减小。

7. 发明授权

US08289050B2 Switching circuits, latches and methods 有权
标题翻译：开关电路，锁存器和方法
公开(公告)号：US08289050B2
公开(公告)日：2012-10-16
申请号：US12887074
申请日：2010-09-21
申请人： John McCoy
发明人： John McCoy
IPC分类号： H03K19/00
CPC分类号： H03K3/0375 , H03K19/0027 , H03K19/01721
摘要： Switching circuits, latches and methods are provided, such as those that may respond to an input signal that transitions from a first binary level to a second binary level. One such switching circuit may have a metastable state that is closer to a first voltage corresponding to the first binary level than it is to a second voltage corresponding to the second binary level. In other embodiments, the metastable state may be dynamically adjustable so that it is at one voltage before the circuit switches and at a different voltage after the circuit switches. As a result, the switching circuit may respond relatively quickly to the input signal transitioning from the first binary level to the second binary level.
摘要翻译：提供了开关电路，锁存器和方法，例如可以响应从第一二进制电平转换到第二二进制电平的输入信号的开关电路，锁存器和方法。一个这样的开关电路可以具有比它对应于第二二进制电平的第二电压更接近对应于第一二进制电平的第一电压的亚稳态。在其他实施例中，亚稳态可以是动态可调节的，使得它在电路切换之前处于一个电压，并且在电路切换之后处于不同的电压。结果，开关电路可以相对快速地响应于从第一二进制电平转换到第二二进制电平的输入信号。

8. 发明申请

US20100204304A1 NOGO Receptor Binding Protein 有权
标题翻译： NOGO受体结合蛋白
公开(公告)号：US20100204304A1
公开(公告)日：2010-08-12
申请号：US12698935
申请日：2010-02-02
申请人： Sha Mi , John McCoy , R. Blake Pepinsky , Daniel H.S. Lee
发明人： Sha Mi , John McCoy , R. Blake Pepinsky , Daniel H.S. Lee
IPC分类号： A61K31/713 , A61K31/7088 , A61P25/00 , A61P25/16 , A61P25/28
CPC分类号： G01N33/56966 , A61K38/00 , C07K14/4702 , C07K14/70571 , C07K16/18 , C07K16/2803 , C07K2319/30 , C12N5/0619 , C12N2500/46 , G01N33/5058 , G01N33/6896
摘要： The invention provides Sp35 polypeptides and fusion proteins thereof, Sp35 antibodies and antigen-binding fragments thereof and nucleic acids encoding the same. The invention also provides compositions comprising, and methods for making and using, such Sp35 antibodies, antigen-binding fragments thereof, Sp35 polypeptides and fusion proteins thereof.
摘要翻译：本发明提供了Sp35多肽及其融合蛋白，Sp35抗体及其抗原结合片段和编码其的核酸。本发明还提供了包含此类Sp35抗体，其抗原结合片段，Sp35多肽及其融合蛋白的制备和使用方法。

9. 发明申请

US20060221604A1 Portable and mobile illumination and detection 失效
标题翻译：便携式和移动照明和检测
公开(公告)号：US20060221604A1
公开(公告)日：2006-10-05
申请号：US11097740
申请日：2005-03-31
申请人： Roy Martin , Brian Zender , John McCoy , David Albers
发明人： Roy Martin , Brian Zender , John McCoy , David Albers
IPC分类号： F21L4/04 , F21V21/088
CPC分类号： F21V21/145 , F21V21/0885
摘要： A mobile illumination apparatus. A universal clamp for attaching to wide range objects such that a broad range of clamping abilities are achieved. Incorporating into the clamp a self-locking ratcheting mechanism for adjusting over a range of spans and fold-out fingers for applying force to the object; the clamp folds up against or into the apparatus for transport or storage. An extendable-and-retractable neck mounting for a transducing-element head thereon, for activating-deactivating the transducing-element, and moving the head to hold an orientation so as to illuminate a region-of-immediate-interest or a particular target. Drive and control circuitry to maintain the illumination intensity substantially constant.
摘要翻译：一种移动式照明装置。用于连接到宽范围物体的通用夹具，从而实现了宽范围的夹紧能力。在夹具中并入一个自锁棘轮机构，用于调节跨越范围和折叠手指以向对象施加力; 夹具折叠抵靠装置进入运输或储存。用于在其上转换元件头部的可伸缩的颈部安装件，用于启动 - 去激活换能元件，以及移动头部以保持取向以照亮即时兴趣区域或特定目标。驱动和控制电路，以保持照明强度基本恒定。

10. 发明申请

US20050066603A1 Internally colored block and process 失效
标题翻译：内部彩色块和过程
公开(公告)号：US20050066603A1
公开(公告)日：2005-03-31
申请号：US10500442
申请日：2003-07-31
申请人： John McCoy , Deborah Elliott
发明人： John McCoy , Deborah Elliott
IPC分类号： E04B5/46 , E04C1/42 , E04C1/39 , E04B5/48 , E04C3/30
CPC分类号： E04B5/46 , E04C1/42
摘要： Internally colored glass and glass-like blocks (10) and the method for making them. No means for coating the internal cavities (26) of clear glass and glass-like hollow blocks has been available. Color coating provides aesthetically pleasing structures. Internally colored glass and glass-like blocks may be used in building internal room dividers, providing colorful building designs and for providing color relief in heretofore otherwise drab structures.
摘要翻译：内部彩色玻璃和玻璃状块（10）及其制作方法。没有用于涂覆透明玻璃和玻璃状中空块的内部空腔（26）的方法已经可用。彩色涂层提供美观的结构。内部彩色玻璃和玻璃状块可用于建造室内分隔器，提供丰富多彩的建筑设计，并在迄今为止以其他方式提供彩色浮雕结构。

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