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2. 发明授权

US07118711B1 Microcolumn reactor 有权
标题翻译：微柱反应器
公开(公告)号：US07118711B1
公开(公告)日：2006-10-10
申请号：US09914874
申请日：2000-02-25
申请人： Johann Michael Koehler
发明人： Johann Michael Koehler
IPC分类号： B01L11/00
CPC分类号： G01N30/56 , B01J19/0046 , B01J19/0093 , B01J2219/00313 , B01J2219/00351 , B01J2219/00423 , B01J2219/00459 , B01J2219/005 , B01J2219/00585 , B01J2219/00596 , B01J2219/00783 , B01J2219/00828 , B01J2219/00833 , B01J2219/00891 , B01J2219/00907 , B01J2219/00952 , B01L3/5027 , C40B60/14 , G01N30/58 , G01N30/6095 , G01N2030/565 , Y10T436/2575
摘要： The invention relates to a microcolumn reactor for carrying out reactions on solid phases and/or with biological cells. The aim of the invention is to provide a microcolumn reactor for processes of synthesis and separation on small sample volumina that replaces an alternating sequence of binding and elution processes on a phase that is stationary bound during a test series and makes it possible to exchange the stationary phase, for example once it has been loaded. To this end, the microcolumn reactor consists of at least one first and one second substrate wafer (1; 2) that are linked with one another across their surfaces. At least one of the substrate wafers (1; 2) is provided with an elongate channel (3) which comprises in a defined section (a) of its length two passage openings (41, 42) that are implemented in the opposite substrate wafer. Said openings (41, 42) are separated from the channel (3) by a partially permeable sieve-type membrane (5). The areas of acceptance (51) of said membrane have a diameter that is chosen in such a manner as to specifically prevent microbeads and/or cells introduced into the channel from entering the passage openings (41, 42). The channel (3) is provided with at least two further openings (61, 62) outside the section (a) that is comprised by the passage openings (41, 42). Said further openings permit the microbeads and/or cells that are provided above the section (a) to be introduced and/or displaced by applying a fluid pressure (p). Means are provided that temporarily close at least one of the passage openings (41, 42) and one of openings (61, 62).
摘要翻译：本发明涉及用于在固相和/或生物细胞上进行反应的微柱反应器。本发明的目的是提供一种微柱反应器，用于在小样品体积上的合成和分离过程，其在测试序列期间在静止界限的相上替代交替的结合和洗脱过程，并且使得可以交换固定相位，例如一旦它被加载。为此，微柱反应器由至少一个在其表面上彼此连接的第一和第二基底晶片（1; 2）组成。衬底晶片（1; 2）中的至少一个设置有细长通道（3），其在其长度的限定部分（a）中包括实现在相对的衬底晶片中的两个通道开口（41,42）。所述开口（41,42）通过部分渗透的筛型膜（5）与通道（3）分离。所述膜的接受区域（51）具有以特定方式选择的直径，以便特定地防止引入通道的微珠和/或细胞进入通道开口（41,42）。通道（3）在由通道开口（41,42）组成的部分（a）外部设置有至少两个另外的开口（61,62）。所述另外的开口允许通过施加流体压力（p）将设置在部分（a）上方的微珠和/或细胞引入和/或移位。提供了临时关闭通道开口（41,42）和开口（61,62）中的至少一个的装置。

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