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1. 发明授权

US07263445B2 Method for the simultaneous dissection in specific positions of filiform organic molecular chains, in particular DNA 有权
标题翻译：丝状有机分子链特别位置同时解剖的方法，特别是DNA
公开(公告)号：US07263445B2
公开(公告)日：2007-08-28
申请号：US10450544
申请日：2001-12-12
申请人： Wolfgang Fritzsche , Karsten Koenig , Johann Michael Koehler
发明人： Wolfgang Fritzsche , Karsten Koenig , Johann Michael Koehler
IPC分类号： B82B3/00 , C12Q1/68
CPC分类号： C12N15/10 , C12Q1/68 , Y10S977/81 , C12Q2563/155 , C12Q2523/313
摘要： The invention relates to a method for the simultaneous dissection in specific positions of filiform organic molecular chains, in particular DNA. The aim of the invention is to provided a method, by which a highly specific dissection can take place on certain sequences that can be freely selected and simultaneously on numerous filiform molecules. To achieve this, nanoparticles (1) are provided with a molecular chain (11) of any predeterminable sequence, which is selected to be complementary to a sequence of a molecule (2) that is to be dissected, said molecular chain(s) (11) is/are hybridised in the usual manner with the molecule, or specifically linked to said molecule in another manner and the nanoparticles (1) are subsequently subjected to a high-energy radiation of at least one wavelength, which can be absorbed by said nanoparticles (1).
摘要翻译：本发明涉及一种用于在丝状有机分子链，特别是DNA的特定位置同时解剖的方法。本发明的目的是提供一种方法，通过该方法可以在可以自由选择并同时存在于许多丝状分子上的某些序列上发生高度特异性的解剖。为了实现这一点，纳米颗粒（1）被提供有任何可预定序列的分子链（11），其被选择为与被解剖的分子（2）的序列互补，所述分子链（ 11）以通常的方式与分子杂交，或以另一种方式特异性连接到所述分子，并且纳米颗粒（1）随后经受至少一个波长的高能辐射，其可被所述纳米粒子（1）。

2. 发明授权

US06878539B1 Affinity sensor for detecting specific molecular binding events and use thereof 失效
标题翻译：用于检测特异性分子结合事件的亲和力传感器及其用途
公开(公告)号：US06878539B1
公开(公告)日：2005-04-12
申请号：US09869206
申请日：1999-12-22
申请人： Wolfgang Fritzsche , Andrea Czaki , Johann Michael Köhler , Antje Wiegand , Louis Oosting , Frederik Schut , Paris Som Tjwan Tan
发明人： Wolfgang Fritzsche , Andrea Czaki , Johann Michael Köhler , Antje Wiegand , Louis Oosting , Frederik Schut , Paris Som Tjwan Tan
IPC分类号： C12M1/00 , C12M1/34 , C12Q1/04 , C12Q1/68 , G01N1/28 , G01N27/02 , G01N27/04 , G01N33/483 , G01N33/543 , G01N33/566 , G01N37/00 , C12M1/42
CPC分类号： G01N33/5438 , B01J2219/00653
摘要： The invention relates to an affinity sensor for detecting specific molecular binding events, for use in the field of molecular biology, e.g., in medical diagnostics, especially in biosensor technology or in DNA microarreay tests. The aim of the invention is to provide an affinity sensor of this type for rapidity, sensitively, specifically, economically and routinely detecting the presence of molecules, especially bioactive molecules, and to provide special applications for an affinity sensor of this type. To this end, the affinity sensor consists of a support substrate which is provided with at least two electrodes. The electrodes are situated equidistantly from each other and cover an area on both sides, at least this area being provided for receiving immobilized specific binding partners which are capable of coupling complementary corresponding binding partners directly or with other specific binding molecules. The area is established with a minimum width b, in such a way that at least one complementary corresponding binding partner which is provided with an electroconductive particle can be received in the area in such a way as to guarantee the possibility of a tunnel-type contact junction forming between the particle and the electrodes in each case. The affinity sensor is used for biomonitoring.
摘要翻译：本发明涉及一种用于检测特定分子结合事件的亲和力传感器，用于分子生物学领域，例如在医学诊断领域，特别是在生物传感器技术或DNA微量测定中。本发明的目的是提供这种类型的亲和力传感器，用于快速，灵敏地，具体地，经济地和常规地检测分子，特别是生物活性分子的存在，并提供这种类型的亲和性传感器的特殊应用。为此，亲和力传感器由设置有至少两个电极的支撑基板组成。电极彼此等距离地覆盖两侧的区域，至少该区域被提供用于接收固定的特异性结合配偶体，其能够直接或与其它特异性结合分子偶联互补的相应结合配偶体。该区域被建立为具有最小宽度b，使得至少一个具有导电颗粒的补充对应的结合伴侣可以被接收在该区域中以保证隧道式接触的可能性在每种情况下，在颗粒和电极之间形成接头。亲和力传感器用于生物监测。

3. 发明申请

US20050176051A1 Affinity sensor for detecting specific molecular binding events and use thereof 审中-公开
标题翻译：用于检测特异性分子结合事件的亲和力传感器及其用途
公开(公告)号：US20050176051A1
公开(公告)日：2005-08-11
申请号：US11066679
申请日：2005-02-24
申请人： Wolfgang Fritzsche , Andrea Czaki , Johann Koehler , Antje Wiegand , Louis Oosting , Frederik Schut , Paris Tjwan Tan
发明人： Wolfgang Fritzsche , Andrea Czaki , Johann Koehler , Antje Wiegand , Louis Oosting , Frederik Schut , Paris Tjwan Tan
IPC分类号： C12M1/00 , C12M1/34 , C12Q1/04 , C12Q1/68 , G01N1/28 , G01N27/02 , G01N27/04 , G01N33/483 , G01N33/543 , G01N33/566 , G01N37/00 , G01N33/53
CPC分类号： G01N33/5438 , B01J2219/00653
摘要： The invention relates to an affinity sensor for detecting specific molecular binding events, for use in the field of molecular biology, e.g., in medical diagnostics, especially in biosensor technology or in DNA microarreay tests. The aim of the invention is to provide an affinity sensor of this type for rapidity, sensitively, specifically, economically and routinely detecting the presence of molecules, especially bioactive molecules, and to provide special applications for an affinity sensor of this type. To this end, the affinity sensor consists of a support substrate which is provided with at least two electrodes. The electrodes are situated equidistantly from each other and cover an area on both sides, at least this area being provided for receiving immobilised specific binding partners which are capable of coupling complementary corresponding binding partners directly or with other specific binding molecules. The area is established with a minimum width b, in such a way that at least one complementary corresponding binding partner which is provided with an electroconductive particle can be received in the area in such a way as to guarantee the possibility of a tunnel-type contact junction forming between the particle and the electrodes in each case. The affinity sensor is used for biomonitoring.
摘要翻译：本发明涉及一种用于检测特定分子结合事件的亲和力传感器，用于分子生物学领域，例如在医学诊断领域，特别是在生物传感器技术或DNA微量测定中。本发明的目的是提供这种类型的亲和力传感器，用于快速，灵敏地，具体地，经济上和常规地检测分子，特别是生物活性分子的存在，并提供这种类型的亲和性传感器的特殊应用。为此，亲和力传感器由设置有至少两个电极的支撑基板组成。电极彼此等距离地覆盖两侧的区域，至少该区域被提供用于接收固定的特异性结合配偶体，其能够直接或与其它特异性结合分子偶联互补的相应结合配偶体。该区域被建立为具有最小宽度b，使得至少一个具有导电颗粒的补充对应的结合伴侣可以被接收在该区域中以保证隧道式接触的可能性在每种情况下，在颗粒和电极之间形成接头。亲和力传感器用于生物监测。

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