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1. 发明申请

US20130065774A1 DNA RECOMBINATION JUNCTION DETECTION 有权
标题翻译： DNA重组接头检测
公开(公告)号：US20130065774A1
公开(公告)日：2013-03-14
申请号：US13455875
申请日：2012-04-25
申请人： Jian Ping Zhang , Ernest Jay Friedlander , Robert Ruhfel , David Douglas Swenson , Alan Thomas Wortman
发明人： Jian Ping Zhang , Ernest Jay Friedlander , Robert Ruhfel , David Douglas Swenson , Alan Thomas Wortman
IPC分类号： C12Q1/68 , C40B30/04
CPC分类号： C12Q1/689 , C12Q1/6858 , C12Q2537/162 , C12Q2537/161
摘要： The present invention provides methods, compositions and kits for detecting the presence or absence of an integrated insertion polynucleotide.
摘要翻译：本发明提供用于检测整合的插入多核苷酸的存在或不存在的方法，组合物和试剂盒。

2. 发明授权

US08187812B2 DNA recombination junction detection 有权
标题翻译： DNA重组结检测
公开(公告)号：US08187812B2
公开(公告)日：2012-05-29
申请号：US11803102
申请日：2007-05-11
申请人： Jian Ping Zhang , Ernest Jay Friedlander , Robert Ruhfel , David Douglas Swenson , Alan Thomas Wortman
发明人： Jian Ping Zhang , Ernest Jay Friedlander , Robert Ruhfel , David Douglas Swenson , Alan Thomas Wortman
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/689 , C12Q1/6858 , C12Q2537/162 , C12Q2537/161
摘要： The present invention provides methods, compositions and kits for detecting the presence or absence of an integrated insertion polynucleotide.
摘要翻译：本发明提供用于检测整合的插入多核苷酸的存在或不存在的方法，组合物和试剂盒。

3. 发明申请

US20090047669A1 DNA recombination junction detection 有权
标题翻译： DNA重组结检测
公开(公告)号：US20090047669A1
公开(公告)日：2009-02-19
申请号：US11803102
申请日：2007-05-11
申请人： Jian Ping Zhang , Ernest Jay Friedlander , Robert Ruhfel , David Douglas Swenson , Alan Thomas Wortman
发明人： Jian Ping Zhang , Ernest Jay Friedlander , Robert Ruhfel , David Douglas Swenson , Alan Thomas Wortman
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q1/6858 , C12Q2537/162 , C12Q2537/161
摘要： The present invention provides methods, compositions and kits for detecting the presence or absence of an integrated insertion polynucleotide.
摘要翻译：本发明提供用于检测整合的插入多核苷酸的存在或不存在的方法，组合物和试剂盒。

4. 发明授权

US08652784B2 DNA recombination junction detection 有权
标题翻译： DNA重组结检测
公开(公告)号：US08652784B2
公开(公告)日：2014-02-18
申请号：US13455875
申请日：2012-04-25
申请人： Jian Ping Zhang , Ernest Jay Friedlander , Robert Ruhfel , David Douglas Swenson , Alan Thomas Wortman
发明人： Jian Ping Zhang , Ernest Jay Friedlander , Robert Ruhfel , David Douglas Swenson , Alan Thomas Wortman
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/689 , C12Q1/6858 , C12Q2537/162 , C12Q2537/161
摘要： The present invention provides methods, compositions and kits for detecting the presence or absence of an integrated insertion polynucleotide.
摘要翻译：本发明提供用于检测整合的插入多核苷酸的存在或不存在的方法，组合物和试剂盒。

5. 发明授权

US06827830B1 Electrophoretic nucleic acid purification method 有权
标题翻译：电泳核酸纯化方法
公开(公告)号：US06827830B1
公开(公告)日：2004-12-07
申请号：US09666905
申请日：2000-09-20
申请人： Gary Slater , J. William Efcavitch , Guy Drouin , Pascal Mayer , Jean Rousseau , Hong Yan Zhou , Claudia Chiesa , Robert Ruhfel , Roger O'Neill
发明人： Gary Slater , J. William Efcavitch , Guy Drouin , Pascal Mayer , Jean Rousseau , Hong Yan Zhou , Claudia Chiesa , Robert Ruhfel , Roger O'Neill
IPC分类号： G01N27447
CPC分类号： C12N15/101 , G01N27/447
摘要： An electrophoretic method for purifying a nucleic acid sample is disclosed. According to the method, the electrophoresis is effective to substantially reduce the concentration of contaminants relative to the concentration of desired nucleic acid in the nucleic acid sample, thereby producing a purified nucleic acid. In the method, the loading and recovery wells may be the same or different, and the electric fields may be DC or alternating.
摘要翻译：公开了一种用于纯化核酸样品的电泳方法。根据该方法，电泳有效地相对于核酸样品中所需核酸的浓度显着降低污染物的浓度，从而产生纯化的核酸。在该方法中，加载和回收井可以相同或不同，并且电场可以是直流或交替的。

6. 发明授权

US6146511A Electrophoretic nucleic acid purification method 失效
标题翻译：电泳核酸纯化方法
公开(公告)号：US6146511A
公开(公告)日：2000-11-14
申请号：US016531
申请日：1998-01-30
申请人： Gary Slater , J. William Efcavitch , Guy Drouin , Pascal Mayer , Jean Rousseau , Hong Yan Zhou , Claudia Chiesa , Robert Ruhfel , Roger O'Neill
发明人： Gary Slater , J. William Efcavitch , Guy Drouin , Pascal Mayer , Jean Rousseau , Hong Yan Zhou , Claudia Chiesa , Robert Ruhfel , Roger O'Neill
IPC分类号： B01D57/02 , B03C5/00 , C07H21/02 , C07H21/04 , C12N15/09 , C12N15/10 , G01N27/447 , G01N27/26
CPC分类号： C12N15/101 , G01N27/447
摘要： An electrophoretic method for purifying a nucleic acid sample is disclosed. The method generally comprises the steps of (1) providing a nucleic acid sample comprising a desired nucleic acid and one or more contaminants, (2) providing an electrophoresis matrix having a loading well and a recovery well formed therein, (3) placing the nucleic acid sample into the loading well, (4) performing a first electrophoresis comprising electrophoresing the nucleic acid sample for a first time effective to transport the desired nucleic acid out of the loading well and into the electrophoresis matrix; and (5) performing a second electrophoresis comprising electrophoresing the nucleic acid sample for a second time effective to transport the desired nucleic acid out of the electrophoresis matrix and into the recovery well. According to the method, the first and second electrophoresis steps are effective to substantially reduce the concentration of contaminants relative to the concentration of desired nucleic acid in the nucleic acid sample, thereby producing a purified nucleic acid. In the method, the loading and recovery wells may be the same or different, and the electric fields may be DC or alternating. Also disclosed is a preparative electrophoresis method employing an alternating electrical field.
摘要翻译：公开了一种用于纯化核酸样品的电泳方法。该方法通常包括以下步骤：（1）提供包含所需核酸和一种或多种污染物的核酸样品，（2）提供具有载体孔的电泳基质和其中形成的回收孔，（3）（4）进行第一次电泳，其中包括使核酸样品首次有效地将所需核酸转运到载体孔中并进入电泳基质中; 包括进行第二次电泳，其包括将核酸样品电泳第二次，有效将所需核酸转运到电泳基质内并进入回收井。根据该方法，第一和第二电泳步骤对于相对于核酸样品中所需核酸浓度显着降低污染物的浓度是有效的，从而产生纯化的核酸。在该方法中，加载和回收井可以相同或不同，并且电场可以是直流或交替的。还公开了采用交流电场的制备电泳方法。

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