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1. 发明申请

US20090176290A1 Bacillus Host Cell 有权
标题翻译：芽孢杆菌宿主细胞
公开(公告)号：US20090176290A1
公开(公告)日：2009-07-09
申请号：US12398803
申请日：2009-03-05
申请人： Jens Tonne Andersen , Steen Troels Jorgensen , Michael Dolbjerg Rasmussen , Peter Bjarke Olsen , Ib Groth Clausen
发明人： Jens Tonne Andersen , Steen Troels Jorgensen , Michael Dolbjerg Rasmussen , Peter Bjarke Olsen , Ib Groth Clausen
IPC分类号： C12P1/04 , C12N1/21
CPC分类号： C07K14/32 , C12N15/75 , C12P1/04 , C12R1/10
摘要： A Bacillus licheniformis mutant host cell derived from a parent B. licheniformis host cell, which mutant host cell is mutated in one or more gene(s) encoding one or more secreted polypeptide(s) which is at least 80% identical to one or more of the polypeptides shown in SEQ ID NO's: 2 to 248, wherein the mutant host cell secretes at least 5% less of the one or more secreted polypeptide(s) than the parent host cell, when they are cultivated under comparable conditions.
摘要翻译：衍生自亲本地衣芽孢杆菌宿主细胞的地衣芽孢杆菌突变宿主细胞，所述突变宿主细胞在编码一种或多种分泌多肽的一个或多个基因中突变，所述多个基因与一种或多种至少80％相同的SEQ ID NO：2至248所示的多肽，其中当它们在可比较的条件下培养时，突变宿主细胞分泌比亲本宿主细胞少至少5％的一种或多种分泌的多肽。

2. 发明授权

US07521204B2 Bacillus host cell 有权
标题翻译：芽孢杆菌宿主细胞
公开(公告)号：US07521204B2
公开(公告)日：2009-04-21
申请号：US10510386
申请日：2003-03-25
申请人： Jens Tonne Andersen , Steen Troels Jorgensen , Michael Dolbjerg Rasmussen , Peter Bjarke Olsen , Ib Groth Clausen
发明人： Jens Tonne Andersen , Steen Troels Jorgensen , Michael Dolbjerg Rasmussen , Peter Bjarke Olsen , Ib Groth Clausen
IPC分类号： C12N15/09 , C12N1/21 , C12N1/20 , C12P1/00
CPC分类号： C07K14/32 , C12N15/75 , C12P1/04 , C12R1/10
摘要： A Bacillus licheniformis mutant host cell derived from a parent B. licheniformis host cell, which mutant host cell is mutated in one or more gene(s) encoding one or more secreted polypeptide(s) which is at least 80% identical to one or more of the polypeptides shown in SEQ ID NO's: 2 to 248, wherein the mutant host cell secretes at least 5% less of the one or more secreted polypeptide(s) than the parent host cell, when they are cultivated under comparable conditions.
摘要翻译：衍生自亲本地衣芽孢杆菌宿主细胞的地衣芽孢杆菌突变宿主细胞，所述突变宿主细胞在编码一种或多种分泌多肽的一个或多个基因中突变，所述多个基因与一种或多种至少80％相同的SEQ ID NO：2至248所示的多肽，其中当它们在可比较的条件下培养时，突变宿主细胞分泌比亲本宿主细胞少至少5％的一种或多种分泌的多肽。

3. 发明授权

US08911969B2 Bacillus host cell 有权
标题翻译：芽孢杆菌宿主细胞
公开(公告)号：US08911969B2
公开(公告)日：2014-12-16
申请号：US12398803
申请日：2009-03-05
申请人： Jens Tonne Andersen , Steen Troels Jorgensen , Michael Dolbjerg Rasmussen , Peter Bjarke Olsen , Ib Groth Clausen
发明人： Jens Tonne Andersen , Steen Troels Jorgensen , Michael Dolbjerg Rasmussen , Peter Bjarke Olsen , Ib Groth Clausen
IPC分类号： C12P21/02 , C12N1/21 , C07K14/32 , C12P1/04 , C12N15/75
CPC分类号： C07K14/32 , C12N15/75 , C12P1/04 , C12R1/10
摘要： A Bacillus licheniformis mutant host cell derived from a parent B. licheniformis host cell, which mutant host cell is mutated in one or more gene(s) encoding one or more secreted polypeptide(s) which is at least 80% identical to one or more of the polypeptides shown in SEQ ID NO's: 2 to 248, wherein the mutant host cell secretes at least 5% less of the one or more secreted polypeptide(s) than the parent host cell, when they are cultivated under comparable conditions.
摘要翻译：衍生自亲本地衣芽孢杆菌宿主细胞的地衣芽孢杆菌突变宿主细胞，所述突变宿主细胞在编码一种或多种分泌多肽的一个或多个基因中突变，所述多个基因与一种或多种至少80％相同的SEQ ID NO：2至248所示的多肽，其中当它们在可比较的条件下培养时，突变宿主细胞分泌比亲本宿主细胞少至少5％的一种或多种分泌的多肽。

4. 发明申请

US20090203074A1 Bacillus Host Cell 审中-公开
公开(公告)号：US20090203074A1
公开(公告)日：2009-08-13
申请号：US12417420
申请日：2009-04-02
申请人： Jens Tonne Andersen , Steen Troels Jorgensen , Michael Dolbjerg Rasmussen , Peter Bjarke Olsen , Ib Groth Clausen
发明人： Jens Tonne Andersen , Steen Troels Jorgensen , Michael Dolbjerg Rasmussen , Peter Bjarke Olsen , Ib Groth Clausen
IPC分类号： C12P1/04 , C12N1/20 , C12N1/21
CPC分类号： C07K14/32 , C12N15/75 , C12P1/04 , C12R1/10
摘要： A Bacillus licheniformis mutant host cell derived from a parent B. licheniformis host cell, which mutant host cell is mutated in one or more gene(s) encoding one or more secreted polypeptide(s) which is at least 80% identical to one or more of the polypeptides shown in SEQ ID NO's: 2 to 248, wherein the mutant host cell secretes at least 5% less of the one or more secreted polypeptide(s) than the parent host cell, when they are cultivated under comparable conditions.

5. 发明授权

US6066473A Introduction of DNA into bacillus strains by conjugation 失效
标题翻译：通过共轭将DNA引入芽孢杆菌菌株
公开(公告)号：US6066473A
公开(公告)日：2000-05-23
申请号：US120576
申请日：1998-07-22
申请人： Martin Tangney , Christian Hansen , Poul Erik Pedersen , Per Lina Jorgensen , Steen Troels Jorgensen
发明人： Martin Tangney , Christian Hansen , Poul Erik Pedersen , Per Lina Jorgensen , Steen Troels Jorgensen
IPC分类号： C12N1/21 , C12N9/54 , C12N15/75 , C12P21/02 , C12N15/64
CPC分类号： C12N15/75 , C12N9/54
摘要： The present invention relates to methods of constructing recombinant Bacillus strains encoding a polypeptide of interest, in which method a DNA construct comprising a DNA sequence encoding the polypeptide of interest is introduced into a recipient Bacillus cell by conjugation from a donor cell.
摘要翻译：本发明涉及构建编码感兴趣多肽的重组芽孢杆菌菌株的方法，其中将包含编码目的多肽的DNA序列的DNA构建体通过从供体细胞缀合引入受体芽孢杆菌细胞中。

6. 发明申请

US20100120623A1 Selection of Well-Expressed Synthetic Genes 有权
标题翻译：选择良好表达的合成基因
公开(公告)号：US20100120623A1
公开(公告)日：2010-05-13
申请号：US12516721
申请日：2007-12-20
申请人： Steen Troels Jorgensen
发明人： Steen Troels Jorgensen
IPC分类号： C40B20/00 , C40B40/08 , C12N1/00 , C12N1/20
CPC分类号： C12N15/1086 , C12N15/67
摘要： The invention relates to a method of indirectly identifying a polynucleotide comprising an open reading frame having an improved expression level from a library of polynucleotides encoding the same polypeptide of interest, or variants or homologues thereof, in a host cell expression library, the method comprising the steps of: a) providing an expression library in a host cell, said library comprising at least two different open reading frames encoding the same polypeptide of interest, or variants or homologues thereof, each open reading frame being translationally coupled to a downstream gene encoding a screenable or selectable reporter; b) culturing the host cell under conditions conducive to the expression of the polypeptide of interest, or variant or homologue thereof; and c) screening and/or selecting for a host cell having an improved expression level of the translationally coupled reporter when compared to one or more other host cells of the library, thus indirectly identifying a polynucleotide comprising an open reading frame having an improved expression level in the host cell; as well as the expression libraries and host cells comprising said libraries.
摘要翻译：本发明涉及一种间接鉴定多核苷酸的方法，该多核苷酸包含在宿主细胞表达文库中从编码相同感兴趣多肽的多核苷酸文库或其变体或同源物的文库中具有改善的表达水平的开放阅读框，所述方法包括步骤：a）在宿主细胞中提供表达文库，所述文库包含编码相同感兴趣多肽的至少两个不同的开放阅读框，或其变体或同源物，每个开放阅读框与下游基因编码一个可筛选或选择的记者; b）在有利于目的多肽表达的条件下或其变体或同源物条件下培养宿主细胞; 和c）当与文库的一个或多个其他宿主细胞相比时，筛选和/或选择具有改良的翻译结合的报道分子的表达水平的宿主细胞，从而间接鉴定包含具有改善的表达水平的开放阅读框的多核苷酸在宿主细胞中; 以及包含所述文库的表达文库和宿主细胞。

7. 发明授权

US5843720A Introduction of DNA into bacillus strains by conjugation 失效
公开(公告)号：US5843720A
公开(公告)日：1998-12-01
申请号：US925927
申请日：1997-09-08
申请人： Martin Tangney , Christian Hansen , Poul Erik Pedersen , Per Lina Jorgensen , Steen Troels Jorgensen
发明人： Martin Tangney , Christian Hansen , Poul Erik Pedersen , Per Lina Jorgensen , Steen Troels Jorgensen
IPC分类号： C12N1/21 , C12N9/54 , C12N15/75 , C12P21/01 , C12N15/64
CPC分类号： C12N15/75 , C12N9/54
摘要： The present invention relates to methods of constructing recombinant Bacillus strains encoding a polypeptide of interest, in which method a DNA construct comprising a DNA sequence encoding the polypeptide of interest is introduced into a recipient Bacillus cell by conjugation from a donor cell.

8. 发明授权

US08431382B2 Chloramphenicol resistance selection in Bacillus licheniformis 有权
公开(公告)号：US08431382B2
公开(公告)日：2013-04-30
申请号：US12302047
申请日：2007-05-29
申请人： Steen Troels Jorgensen
发明人： Steen Troels Jorgensen
IPC分类号： C12N1/20 , C12N15/74 , C12N9/10 , C12N15/00 , C12Q1/68 , C12P21/06 , C07H21/04
CPC分类号： C12N15/75 , C12N9/1033
摘要： The present invention relates to a modified Bacillus licheniformis host cell, wherein one or more naturally occurring chromosomal chloramphenicol acetyl transferase encoding gene(s), cat, has been inactivated. The inactivation of the chromosomal cat gene(s) allows the use of chloramphenicol as an efficient selective agent in methods for DNA introduction into the modified cell.

9. 发明申请

US20090280569A1 Chloramphenicol Resistance Selection in Bacillus Licheniformis 有权
标题翻译：地衣芽孢杆菌中氯霉素抗性选择
公开(公告)号：US20090280569A1
公开(公告)日：2009-11-12
申请号：US12302047
申请日：2007-05-29
申请人： Steen Troels Jorgensen
发明人： Steen Troels Jorgensen
IPC分类号： C12N15/75 , C12N1/21
CPC分类号： C12N15/75 , C12N9/1033
摘要： The present invention relates to a modified Bacillus licheniformis host cell, wherein one or more naturally occurring chromosomal chloramphenicol acetyl transferase encoding gene(s), cat, has been inactivated. The inactivation of the chromosomal cat gene(s) allows the use of chloramphenicol as an efficient selective agent in methods for DNA introduction into the modified cell.
摘要翻译：本发明涉及一种修饰的地衣芽孢杆菌宿主细胞，其中一个或多个天然存在的染色体氯霉素乙酰转移酶编码基因（cat）已被灭活。染色体cat基因的失活允许在将DNA引入修饰细胞的方法中使用氯霉素作为有效的选择剂。

10. 发明授权

US07157262B2 Lipolytic enzymes 失效
标题翻译：脂肪分解酶
公开(公告)号：US07157262B2
公开(公告)日：2007-01-02
申请号：US10232544
申请日：2002-08-30
申请人： Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
发明人： Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
IPC分类号： C12N9/20 , C12N9/16 , C12N1/20 , C12N15/00 , C12P21/06
CPC分类号： C11D3/38627 , C12N9/20
摘要： The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzyme capable of removing a substantial amount of fatty matter during a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.
摘要翻译：本发明涉及具有脂肪分解活性的修饰酶，能够在一次循环洗涤期间除去大量脂肪物质的脂肪分解酶，编码所述酶的DNA序列，包含所述DNA序列的载体，携带所述DNA的宿主细胞序列或所述载体，以及用于产生具有脂肪分解活性的所述酶的方法。

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