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    • 1. 发明授权
    • PNA probes, probe sets, methods and kits pertaining to the detection of Candida
    • PNA探针,探针组,与念珠菌检测有关的方法和试剂盒
    • US08912312B2
    • 2014-12-16
    • US13207283
    • 2011-08-10
    • Jens J. Hyldig-NielsenHenrik StenderKenneth M. OliveiraSusan Rigby
    • Jens J. Hyldig-NielsenHenrik StenderKenneth M. OliveiraSusan Rigby
    • C12Q1/68C07K14/00
    • C12Q1/6895C07K14/003C12Q1/689C12Q2525/107
    • This invention is related to novel PNA probes, probe sets, methods and kits pertaining to the detection of one or more species of Candida yeast. Non-limiting examples of probing nucleobase sequences that can be used for the probes of this invention can be selected from the group consisting of: AGA-GAG-CAG-CAT-GCA (Seq. Id. No. 1), AGA-GAG-CAA-CAT-GCA (Seq. Id. No. 2), ACA-GCA-GAA-GCC-GTG (Seq. Id. No. 3), CAT-AAA-TGG-CTA-CCA-GA (Seq. Id. No. 4), CAT-AAA-TGG-CTA-CCC-AG (Seq. Id. No. 5), ACT-TGG-AGT-CGA-TAG (Seq. Id. No. 6), CCA-AGG-CTT-ATA-CTC-GC (Seq. Id. No. 7), CCC-CTG-AAT-CGG-GAT (Seq. Id. No. 8), GAC-GCC-AAA-GAC-GCC (Seq. Id. No. 9), ATC-GTC-AGA-GGC-TAT-AA (Seq. Id. No. 10), TAG-CCA-GAA-GAA-AGG (Seq. Id. No. 11), CAT-AAA-TGG-CTA-GCC-AG (Seq. Id. No. 12), CTC-CGA-TGT-GAC-TGC-G (Seq. Id. No. 13), TCC-CAG-ACT-GCT-CGG (Seq. Id. No. 14), TCC-AAG-AGG-TCG-AGA (Seq. Id. No. 15), GCC-AAG-CCA-CAA-GGA (Seq. Id. No. 16), GCC-GCC-AAG-CCA-CA (Seq. Id. No. 17), GGA-CTT-GGG-GTT-AG (Seq. Id. No. 18), CCG-GGT-GCA-TTC-CA (Seq. Id. No. 19), ATG-TAG-AAC-GGA-ACT-A (Seq. Id. No. 20), GAT-TCT-CGG-CCC-CAT-G (Seq. Id. No. 21), CTG-GTT-CGC-CAA-AAA-G (Seq. Id. No. 22) and AGT-ACG-CAT-CAG-AAA (Seq. Id. No. 23).
    • 本发明涉及与一种或多种念珠菌酵母的检测有关的新型PNA探针,探针组,方法和试剂盒。 可用于本发明探针的探测核碱基序列的非限制性实例可以选自:AGA-GAG-CAG-CAT-GCA(Seq.Id。No.1),AGA-GAG- CAA-CAT-GCA(序列号2),ACA-GCA-GAA-GCC-GTG(序列号3),CAT-AAA-TGG-CTA-CCA-GA(Seq.Id。 第4号),CAT-AAA-TGG-CTA-CCC-AG(Seq.Id。No.5),ACT-TGG-AGT-CGA-TAG(Seq.Id。No.6),CCA-AGG-CTT -ATA-CTC-GC(Seq.Id。No.7),CCC-CTG-AAT-CGG-GAT(Seq.Id。No.8),GAC-GCC-AAA-GAC-GCC(Seq.Id.No ATC-GTC-AGA-GGC-TAT-AA(序列号10),TAG-CCA-GAA-GAA-AGG(序列号11),CAT-AAA-TGG- CTA-GCC-AG(序列号12),CTC-CGA-TGT-GAC-TGC-G(序列号13),TCC-CAG-ACT-GCT-CGG(Seq.Id。 第14号),TCC-AAG-AGG-TCG-AGA(序列号15),GCC-AAG-CCA-CAA-GGA(序列号16),GCC-GCC-AAG-CCA -CA(Seq.Id。No.17),GGA-CTT-GGG-GTT-AG(Seq.Id.18),CCG-GGT-GCA-TTC-CA(Seq.Id.No.19) ATG-TAG-AAC-GGA-ACT-A(序列号20),GAT-TCT-CGG-CCC-CAT-G(序列号21),CTG-GTT-CGC-CAA- AAA-G (Seq。 ID。 No.22)和AGT-ACG-CAT-CAG-AAA(Seq.Id。No.23)。
    • 2. 发明授权
    • PNA probes, probe sets, methods and kits pertaining to the detection of Candida
    • PNA探针,探针组,与念珠菌检测有关的方法和试剂盒
    • US08026051B2
    • 2011-09-27
    • US10150045
    • 2002-05-17
    • Jens J. Hyldig-NielsenHenrik StenderKenneth M. OliveiraSusan Rigby
    • Jens J. Hyldig-NielsenHenrik StenderKenneth M. OliveiraSusan Rigby
    • C07H21/04C12Q1/68
    • C12Q1/6895C07K14/003C12Q1/689C12Q2525/107
    • This invention is related to novel PNA probes, probe sets, methods and kits pertaining to the detection of one or more species of Candida yeast. Non-limiting examples of probing nucleobase sequences that can be used for the probes of this invention can be selected from the group consisting of: AGA-GAG-CAG-CAT-GCA (Seq. Id. No. 1), AGA-GAG-CAA-CAT-GCA (Seq. Id. No. 2), ACA-GCA-GAA-GCC-GTG (Seq. Id. No. 3), CAT-AAA-TGG-CTA-CCA-GA (Seq. Id. No. 4), CAT-AAA-TGG-CTA-CCC-AG (Seq. Id. No. 5), ACT-TGG-AGT-CGA-TAG (Seq. Id. No. 6), CCA-AGG-CTT-ATA-CTC-GC (Seq. Id. No. 7), CCC-CTG-AAT-CGG-GAT (Seq. Id. No. 8), GAC-GCC-AAA-GAC-GCC (Seq. Id. No. 9), ATC-GTC-AGA-GGC-TAT-AA (Seq. Id. No. 10), TAG-CCA-GAA-GAA-AGG (Seq. Id. No. 11), CAT-AAA-TGG-CTA-GCC-AG (Seq. Id. No. 12), CTC-CGA-TGT-GAC-TGC-G (Seq. Id. No. 13), TCC-CAG-ACT-GCT-CGG (Seq. Id. No. 14), TCC-AAG-AGG-TCG-AGA (Seq. Id. No. 15), GCC-AAG-CCA-CAA-GGA (Seq. Id. No. 16), GCC-GCC-AAG-CCA-CA (Seq. Id. No. 17), GGA-CTT-GGG-GTT-AG (Seq. Id. No. 18), CCG-GGT-GCA-TTC-CA (Seq. Id. No. 19), ATG-TAG-AAC-GGA-ACT-A (Seq. Id. No. 20), GAT-TCT-CGG-CCC-CAT-G (Seq. Id. No. 21), CTG-GTT-CGC-CAA-AAA-G (Seq. Id. No. 22) and AGT-ACG-CAT-CAG-AAA (Seq. Id. No. 23).
    • 本发明涉及与一种或多种念珠菌酵母的检测有关的新型PNA探针,探针组,方法和试剂盒。 可用于本发明探针的探测核碱基序列的非限制性实例可以选自:AGA-GAG-CAG-CAT-GCA(Seq.Id。No.1),AGA-GAG- CAA-CAT-GCA(序列号2),ACA-GCA-GAA-GCC-GTG(序列号3),CAT-AAA-TGG-CTA-CCA-GA(Seq.Id。 第4号),CAT-AAA-TGG-CTA-CCC-AG(Seq.Id。No.5),ACT-TGG-AGT-CGA-TAG(Seq.Id。No.6),CCA-AGG-CTT -ATA-CTC-GC(Seq.Id。No.7),CCC-CTG-AAT-CGG-GAT(Seq.Id。No.8),GAC-GCC-AAA-GAC-GCC(Seq.Id.No ATC-GTC-AGA-GGC-TAT-AA(序列号10),TAG-CCA-GAA-GAA-AGG(序列号11),CAT-AAA-TGG- CTA-GCC-AG(序列号12),CTC-CGA-TGT-GAC-TGC-G(序列号13),TCC-CAG-ACT-GCT-CGG(Seq.Id。 第14号),TCC-AAG-AGG-TCG-AGA(序列号15),GCC-AAG-CCA-CAA-GGA(序列号16),GCC-GCC-AAG-CCA -CA(Seq.Id。No.17),GGA-CTT-GGG-GTT-AG(Seq.Id.18),CCG-GGT-GCA-TTC-CA(Seq.Id.No.19) ATG-TAG-AAC-GGA-ACT-A(序列号20),GAT-TCT-CGG-CCC-CAT-G(序列号21),CTG-GTT-CGC-CAA- AAA-G (Seq。 ID。 No.22)和AGT-ACG-CAT-CAG-AAA(Seq.Id。No.23)。
    • 4. 发明授权
    • Methods for the detection, identification, and/or enumeration of yeast, particularly in wine
    • 检测,鉴定和/或计数酵母,特别是葡萄酒的方法
    • US07060432B1
    • 2006-06-13
    • US09593914
    • 2000-06-14
    • Jens J. Hyldig-NielsenHeather P. O'KeefeHenrik Stender
    • Jens J. Hyldig-NielsenHeather P. O'KeefeHenrik Stender
    • C12Q1/68C07H21/02C07H21/04
    • C12Q1/6895
    • This invention is related to novel probes, probe sets, methods and kits pertaining to the detection, identification and/or quantitation of yeasts and particularly Dekkera bruxellensis (a.k.a. Brettanomyces) an organism that spoils wine. Preferred probes for the detection of one or more species of the Dekkera/Brettanomyces genus comprise a probing nucleobase sequence, at least a portion of which is selected from the group consisting of: AGC-GGG-TCT-ATT-AGA (Seq. ID No. 1); CCA-GGT-GAG-GGT-CGC (Seq. ID No. 2); CGG-TTG-CCC-GAT-TTC (Seq. ID No. 3); TCG-CCT-TCC-TCC-TCT (Seq. ID No. 4); CGG-TCT-CCA-GCG-ATT (Seq. ID No. 5); CAC-AAG-ATG-TCC-GCG (Seq. ID No. 6); GCG-GGC-ACT-AAT-TGA (Seq. ID No. 7); CAT-CCA-CGA-GGA-ACG (Seq. ID No. 8); GTG-TAA-ACC-AGG-TGC (Seq. ID No. 9); ATG-GCT-CCC-AGA-ACC (Seq. ID No. 10) and GAC-AGA-ATC-GAA-GGG (Seq. ID No. 11). The probes, probe sets, methods and kits of this invention are particularly well suited for use in the analysis of yeast in wine, beer and liquor and in the monitoring of contamination of in-process product as well as the equipment and facilities used to manufacture product in wineries and breweries.
    • 本发明涉及关于酵母的特异性检测,鉴定和/或定量的新型探针,探针组,方法和试剂盒,尤其是破坏葡萄酒的生物的德克萨维酵母(A.k.a.Brettanomyces)。 用于检测一种或多种Dekkera / Brettanomyces属的优选探针包括探测核碱基序列,其至少一部分选自:AGC-GGG-TCT-ATT-AGA(Seq.ID No 1); CCA-GGT-GAG-GGT-CGC(Seq.ID No.2); CGG-TTG-CCC-GAT-TTC(Seq.ID No.3); TCG-CCT-TCC-TCC-TCT(Seq.ID No.4); CGG-TCT-CCA-GCG-ATT(Seq.ID No.5); CAC-AAG-ATG-TCC-GCG(Seq.ID No.6); GCG-GGC-ACT-AAT-TGA(Seq.ID No.7); CAT-CCA-CGA-GGA-ACG(Seq.ID No.8); GTG-TAA-ACC-AGG-TGC(Seq.ID No.9); ATG-GCT-CCC-AGA-ACC(Seq.ID No.10)和GAC-AGA-ATC-GAA-GGG(Seq.ID No.11)。 本发明的探针,探针组,方法和试剂盒特别适合用于分析葡萄酒,啤酒和酒中的酵母,以及监测过程中产品的污染以及用于制造的设备和设备 产品在酿酒厂和啤酒厂。