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1. 发明授权

US06613959B1 Transgenic plants expressing a MAPKKK protein kinase domain 失效
标题翻译：表达MAPKKK蛋白激酶结构域的转基因植物
公开(公告)号：US06613959B1
公开(公告)日：2003-09-02
申请号：US09371338
申请日：1999-08-10
申请人： Jen Sheen , Yelena V. Kovtun , Wan-Ling Chiu
发明人： Jen Sheen , Yelena V. Kovtun , Wan-Ling Chiu
IPC分类号： C12N1509
CPC分类号： C12N9/1205 , C12N9/16 , C12N15/8266 , C12N15/827 , C12N15/8271 , C12N15/8287 , C12N15/8294
摘要： The invention features a method for increasing stress resistance or tolerance in a plant, the method including the steps of: (a) introducing into plant cells a transgene including DNA encoding a kinase domain of a mitogen-activated protein kinase kinase kinase (MAPKKK) operably linked to a promoter functional in plant cells to yield transformed plant cells; and (b) regenerating a transgenic plant from the transformed cells, wherein the kinase domain of said MAPKKK is expressed in the cells of the transgenic plant, thereby increasing the level of stress resistance or tolerance in the transgenic plant. The invention further features plants including a recombinant transgene capable of expressing a kinase domain of a mitogen-activated protein kinase kinase kinase (MAPKKK) or a kinase domain thereof, wherein the transgene is expressed in said plant under the control of a promoter that is functional in a plant cell.
摘要翻译：本发明的特征在于增加植物的胁迫抗性或耐受性的方法，所述方法包括以下步骤：（a）可操作地向植物细胞中引入包含编码丝裂原活化蛋白激酶激酶激酶（MAPKKK）的激酶结构域的DNA的转基因与在植物细胞中起作用的启动子连接以产生转化的植物细胞; 和（b）从转化的细胞再生转基因植物，其中所述MAPKKK的激酶结构域在转基因植物的细胞中表达，由此增加转基因植物的胁迫抗性或耐受性水平。本发明还涉及包括能够表达丝裂原活化蛋白激酶激酶激酶（MAPKKK）或其激酶结构域的激酶结构域的重组转基因的植物，其中所述植物在所述植物中表达，所述启动子是功能性的在植物细胞中。

2. 发明授权

US06548743B1 Transgenic plants expressing a dual-specificity MAPK phosphatase and uses thereof 失效
标题翻译：表达双特异性MAPK磷酸酶的转基因植物及其用途
公开(公告)号：US06548743B1
公开(公告)日：2003-04-15
申请号：US09371671
申请日：1999-08-10
申请人： Jen Sheen , Wan-Ling Chiu
发明人： Jen Sheen , Wan-Ling Chiu
IPC分类号： A01H500
CPC分类号： C12N9/1205 , C12N9/16 , C12N15/8266 , C12N15/827 , C12N15/8294
摘要： Disclosed are transgenic plants which include a transgene which expresses a phosphatase domain of a dual-specificity mitogen-activated protein kinase (MAPK) phosphatase, wherein the transgene is expressed in the transgenic plant under the control of a promoter that is functional in a plant cell. Also disclosed are methods which are useful for modifying the phenotype of a plant, including the steps of: (a) introducing into a plant cell a transgene comprising DNA encoding a phosphatase domain of a dual-specificity mitogen-activating protein kinase (MAPK) phosphatase operably linked to a promoter functional in plant cells to yield a transformed plant cell; and (b) regenerating a transgenic plant from the transformed cells, wherein the phosphatase domain of the dual-specificity MAPK phosphatase is expressed in the cells of the transgenic plant, thereby modifying the phenotype of the transgenic plant.
摘要翻译：披露的是转基因植物，其包括表达双特异性丝裂原活化蛋白激酶（MAPK）磷酸酶的磷酸酶结构域的转基因，其中转基因在转基因植物中表达，其在植物细胞中起作用的启动子。还公开了可用于修饰植物表型的方法，包括以下步骤：（a）将包含编码双特异性丝裂原活化蛋白激酶（MAPK）磷酸酶的磷酸酶结构域的DNA的转基因导入植物细胞可操作地连接到在植物细胞中起作用的启动子以产生转化的植物细胞; 和（b）从转化的细胞再生转基因植物，其中双特异性MAPK磷酸酶的磷酸酶结构域在转基因植物的细胞中表达，由此修饰转基因植物的表型。

3. 发明申请

US20060185030A1 Cytokinin response regulators and uses thereof 审中-公开
标题翻译：细胞分裂素反应调节剂及其用途
公开(公告)号：US20060185030A1
公开(公告)日：2006-08-17
申请号：US10479834
申请日：2002-06-06
申请人： Jen Sheen , Ildoo Hwang
发明人： Jen Sheen , Ildoo Hwang
IPC分类号： A01H1/00 , C12N5/04 , C12N15/82
CPC分类号： C07K14/415 , C12N15/8295
摘要： The invention generally features methods for increasing yield, shoot formation, and delaying senesence in plants with the use of transgenes that regulate the cytokinin response. The invention also features plants and plant components that harbor the transgene(s).
摘要翻译：本发明通常具有使用调节细胞分裂素反应的转基因增加植物产量，芽形成和延迟衰老的方法。本发明还涉及含有转基因的植物和植物成分。

4. 发明授权

US06639130B2 Plant sterol reductases and uses thereof 失效
标题翻译：植物甾醇还原酶及其用途
公开(公告)号：US06639130B2
公开(公告)日：2003-10-28
申请号：US08879337
申请日：1997-06-20
申请人： Jyan-Chyun Jang , Jen Sheen
发明人： Jyan-Chyun Jang , Jen Sheen
IPC分类号： A01H500
CPC分类号： C12N9/001 , C12N9/0006 , C12N15/8243 , C12N15/8291 , C12Q1/26 , G01N33/573
摘要： Disclosed are plant DNA molecules encoding C-14 sterol reductase polypeptides, as well as plant DNA molecules encoding polypeptides having at least 50% identity to the Arabidopsis C-14 sterol reductase; and vectors, cells and plants expressing such DNA. Also provided are methods for modifying the phenotype of a plant by expressing a C-14 sterol reductase DNA molecule in either sense or antisense orientations.
摘要翻译：公开了编码C-14固醇还原酶多肽的植物DNA分子以及编码与拟南芥C-14甾醇还原酶至少50％同一性的多肽的植物DNA分子; 和表达这种DNA的载体，细胞和植物。还提供了通过以正义或反义取向表达C-14固醇还原酶DNA分子来修饰植物表型的方法。

5. 发明授权

US06632602B1 Plant sugar sensors and uses thereof 失效
标题翻译：植物糖传感器及其用途
公开(公告)号：US06632602B1
公开(公告)日：2003-10-14
申请号：US08622191
申请日：1996-03-25
申请人： Jen Sheen , Jyan-Chyun Jang
发明人： Jen Sheen , Jyan-Chyun Jang
IPC分类号： C12N1500
CPC分类号： C12N9/1205 , C12N15/8241 , C12N15/8245
摘要： Disclosed are methods and genes for manipulating the sugar-sensing capabilities of a plant, involving reducing or increasing the level of a hexokinase protein in a transgenic plant.
摘要翻译：公开了用于操纵植物的糖感测能力的方法和基因，涉及降低或提高转基因植物中己糖激酶蛋白的水平。

6. 发明申请

US20050262584A1 Master activators of pathogen responsive genes 审中-公开
标题翻译：病原体响应基因的主要激活剂
公开(公告)号：US20050262584A1
公开(公告)日：2005-11-24
申请号：US10661426
申请日：2003-09-12
申请人： Jen Sheen , Frederick Ausubel , Tsuneaki Asai , Guillaume Tena
发明人： Jen Sheen , Frederick Ausubel , Tsuneaki Asai , Guillaume Tena
IPC分类号： A01H1/00 , C07K14/47 , C12N9/12 , C12N15/29 , C12N15/82
CPC分类号： C12N15/8281 , C07K14/4705 , C12N9/1205 , C12N15/8282
摘要： Disclosed is a complete plant MAP kinase cascade (e.g., MEKK1, MKK4/MKK5, and MPK3/MPK6) and WRKY22/WRKY29 transcription factors that function downstream of the flagellin receptor FLS2, a leucine-rich-repeat (LRR) receptor kinase. Activation of such a MAPK cascade confers resistance to both bacterial and fungal pathogens. Also disclosed are disease-resistant plants expressing one or more members of this MAP kinase signaling cascade. Such members include constitutively active MEKK1 (ΔMEKK1), MKK4 (MKK4a), and MKK5 (MKK5a) or wild-type WRKY29.
摘要翻译：公开了在富含亮氨酸重复（LRR）受体激酶的鞭毛蛋白受体FLS2的下游起作用的完整植物MAP激酶级联（例如MEKK1，MKK4 / MKK5和MPK3 / MPK6）和WRKY22 / WRKY29转录因子。这种MAPK级联的活化赋予细菌和真菌病原体的抗性。还公开了表达该MAP激酶信号级联的一个或多个成员的抗病植物。这些成员包括组成型活性的MEKK1（DeltaMEKK1），MKK4（MKK4a）和MKK5（MKK5a）或野生型WRKY29。

7. 发明申请

US20090019602A1 MASTER ACTIVATORS OF PATHOGEN RESPONSIVE GENES 审中-公开
标题翻译：病原体基因的主要激活剂
公开(公告)号：US20090019602A1
公开(公告)日：2009-01-15
申请号：US12143712
申请日：2008-06-20
申请人： Jen Sheen , Frederick M. Ausubel , Tsuneaki Asai , Guillaume Tena
发明人： Jen Sheen , Frederick M. Ausubel , Tsuneaki Asai , Guillaume Tena
IPC分类号： C12N15/54
CPC分类号： C12N15/8281 , C07K14/4705 , C12N9/1205 , C12N15/8282
摘要： Disclosed is a complete plant MAP kinase cascade (e.g., MEKK1, MKK4/MKK5, and MPK3/MPK6) and WRKY22/WRKY29 transcription factors that function downstream of the flagellin receptor FLS2, a leucine-rich-repeat (LRR) receptor kinase. Activation of such a MAPK cascade confers resistance to both bacterial and fungal pathogens. Also disclosed are disease-resistant plants expressing one or more members of this MAP kinase signaling cascade. Such members include constitutively active MEKK1 (ΔMEKK1), MKK4 (MKK4a), and MKK5 (MKK5a) or wild-type WRKY29.
摘要翻译：公开了在富含亮氨酸重复（LRR）受体激酶的鞭毛蛋白受体FLS2的下游起作用的完整植物MAP激酶级联（例如MEKK1，MKK4 / MKK5和MPK3 / MPK6）和WRKY22 / WRKY29转录因子。这种MAPK级联的活化赋予细菌和真菌病原体的抗性。还公开了表达该MAP激酶信号级联的一个或多个成员的抗病植物。这些成员包括组成型活性的MEKK1（DeltaMEKK1），MKK4（MKK4a）和MKK5（MKK5a）或野生型WRKY29。

8. 发明授权

US07084323B1 Stress-protected transgenic plants 失效
标题翻译：受胁迫保护的转基因植物
公开(公告)号：US07084323B1
公开(公告)日：2006-08-01
申请号：US08989881
申请日：1997-12-12
申请人： Jen Sheen
发明人： Jen Sheen
IPC分类号： C12N15/82 , C12N15/29 , A01H5/00 , A01H5/10
CPC分类号： C12N9/1205 , C12N15/8273
摘要： Disclosed are methods for increasing a plant's tolerance to environmental stress.
摘要翻译：公开了增加植物对环境胁迫耐受性的方法。

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