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1. 发明授权

US08372605B2 Immuno-amplification 有权
标题翻译：免疫扩增
公开(公告)号：US08372605B2
公开(公告)日：2013-02-12
申请号：US13072314
申请日：2011-03-25
申请人： James Nadeau , Tobin J. Hellyer , Dolores M. Berger , William Nussbaumer , Robert Rosenstein , Andrew Kuhn , Sha-Sha Wang , Keith Edward Thornton
发明人： James Nadeau , Tobin J. Hellyer , Dolores M. Berger , William Nussbaumer , Robert Rosenstein , Andrew Kuhn , Sha-Sha Wang , Keith Edward Thornton
IPC分类号： C12P19/34 , C12Q1/68 , G01N33/53 , C07H21/04 , C07K16/00
CPC分类号： C12Q1/6804 , C12Q1/6851
摘要： A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte.
摘要翻译：提供了高灵敏度，低背景的免疫扩增测定法，其提供流线型工作流程，适用于临床相关样品如血液和其他体液的高通量测定。该测定包括使用两个邻近成员，每个邻近成员包含与寡核苷酸缀合的分析物特异性结合成分。结合分析物使邻近成员的寡核苷酸部分充分紧密接触，使寡核苷酸形成扩增子。然后通过扩增扩增子和扩增核酸的检测来检测分析物的存在。通过防止与分析物不复合的邻近成员形成假的或非特异性的扩增子来提高本发明的测定的灵敏度。

2. 发明申请

US20110244457A1 IMMUNO-AMPLIFICATION 有权
标题翻译：免疫放大
公开(公告)号：US20110244457A1
公开(公告)日：2011-10-06
申请号：US13072314
申请日：2011-03-25
申请人： James Nadeau , Tobin Hellyer , Dolores M. Berger , William Nussbaumer , Robert Rosenstein , Andrew Kuhn , Sha Sha Wang , Keith Thornton
发明人： James Nadeau , Tobin Hellyer , Dolores M. Berger , William Nussbaumer , Robert Rosenstein , Andrew Kuhn , Sha Sha Wang , Keith Thornton
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6804 , C12Q1/6851
摘要： A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte.
摘要翻译：提供了高灵敏度，低背景的免疫扩增测定法，其提供流线型工作流程，适用于临床相关样品如血液和其他体液的高通量测定。该测定包括使用两个邻近成员，每个邻近成员包含与寡核苷酸缀合的分析物特异性结合成分。结合分析物使邻近成员的寡核苷酸部分充分紧密接触，使寡核苷酸形成扩增子。然后通过扩增扩增子和扩增核酸的检测来检测分析物的存在。通过防止与分析物不复合的邻近成员形成假的或非特异性的扩增子来提高本发明的测定的灵敏度。

3. 发明授权

US06583279B1 Sequences and methods for detection of hepatitis B virus 有权
标题翻译：检测乙型肝炎病毒的序列和方法
公开(公告)号：US06583279B1
公开(公告)日：2003-06-24
申请号：US09770532
申请日：2001-01-26
申请人： Dolores M. Berger , William A. Nussbaumer , Thomas L. Fort , Tobin J. Hellyer
发明人： Dolores M. Berger , William A. Nussbaumer , Thomas L. Fort , Tobin J. Hellyer
IPC分类号： C07H2100
CPC分类号： C12Q1/706
摘要： Primers and probes derived from the HBV DNA polymerase gene which facilitate detection and/or quantification of all presently known genotypes of HBV. Disclosed sequences may be used in a variety of primer and probe constructs for detection of HBV nucleic acids.
摘要翻译：源自HBV DNA聚合酶基因的引物和探针，其便于检测和/或定量所有目前已知的HBV基因型。公开的序列可以用于各种用于检测HBV核酸的引物和探针构建体。

4. 发明授权

US06218125B1 Assay for Chlamydia trachomatis by amplification and detection of Chlamydia trachomatis cryptic plasmid 有权
标题翻译：通过扩增和检测沙眼衣原体隐窝质粒检测沙眼衣原体
公开(公告)号：US06218125B1
公开(公告)日：2001-04-17
申请号：US09481810
申请日：2000-01-12
申请人： Paul A. Foxall , Dolores M. Berger
发明人： Paul A. Foxall , Dolores M. Berger
IPC分类号： C12Q168
CPC分类号： C12Q1/689
摘要： A region of the Chlamydia trachomatis cryptic plasmid has been identified which is useful for performing amplification assays to determine specifically whether C. trachomatis is present in the sample being tested. Oligonucleotides useful for performing thermal Strand Displacement Assay (tSDA) reactions on this gene are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for all strains of C. trachomatis and which does not show crossreactivity with the genomes of other microorganisms or with human DNA.
摘要翻译：已经鉴定了沙眼衣原体隐窝质粒的区域，其可用于进行扩增测定，以确定是否存在待测样品中的沙眼衣原体。公开了用于对该基因进行热链置换测定（tSDA）反应的寡核苷酸。所公开的寡核苷酸可用于对沙眼衣原体的所有菌株特异性并且不显示与其他微生物的基因组或与人类DNA的交叉反应性的测定。

5. 发明授权

US5667994A Amplification and detection of mycobacterium avium complex species 失效
标题翻译：鸟分枝杆菌复合物的扩增和检测
公开(公告)号：US5667994A
公开(公告)日：1997-09-16
申请号：US608584
申请日：1996-02-28
申请人： Karen Ann Dilly , Silvia A. Bustos , Christine Ann Rostkowski , Dolores M. Berger
发明人： Karen Ann Dilly , Silvia A. Bustos , Christine Ann Rostkowski , Dolores M. Berger
IPC分类号： C12N15/09 , C07H21/04 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/689 , C12Q2600/16
摘要： Amplification primers and methods for complex-specific amplification of target sequences in the dnaJ genes of the Mycobacterium avium Complex (MAC) species are disclosed. The primer target binding sequences are useful for amplification of the dnaJ target in a variety of amplification reactions, with detection of the complex-specific target and, optionally, identification of the MAC species from which the target is derived. Primers and methods for multiplex amplification of dnaJ and a second target are also described.
摘要翻译：公开了扩增引物和用于复合特异性扩增鸟分枝杆菌复合物（MAC）物种的dnaJ基因中靶序列的方法。引物靶结合序列可用于在多种扩增反应中扩增dnaJ靶，检测复合物特异性靶标，以及任选地鉴定衍生靶标的MAC物种。还描述了dnaJ和第二靶的多重扩增的引物和方法。

6. 发明授权

US06916608B2 Composition for providing long term stability to cells for diagnostic testing 失效
标题翻译：为细胞提供长期稳定性用于诊断测试的组合物
公开(公告)号：US06916608B2
公开(公告)日：2005-07-12
申请号：US09394006
申请日：1999-09-10
申请人： Dolores M. Berger , Daretta A. Yursis , William A. Nussbaumer , Anne B. Brown
发明人： Dolores M. Berger , Daretta A. Yursis , William A. Nussbaumer , Anne B. Brown
IPC分类号： C12N15/09 , C12N1/04 , C12Q1/68 , C12R1/725 , G01N1/30
CPC分类号： C12Q1/6806
摘要： The present invention relates to a method and composition for stabilizing clinical specimens (i.e., cells in biological samples) for transport and subsequent testing for diagnosis. The composition is specifically capable of maintaining nucleic acid in the cells intact for hybridization with oligonucleotide capture and detector probes.
摘要翻译：本发明涉及用于稳定临床标本（即，生物样品中的细胞）用于运输和随后的诊断测试的方法和组合物。组合物特异性能够保持细胞中的核酸完整以与寡核苷酸捕获和检测器探针杂交。

7. 发明授权

US6096501A Assay for Chlamydia trachomatis by amplification and detection of Chlamydia trachomatis crytpic plasmid 失效
标题翻译：沙眼衣原体沙眼衣原体通过扩增和检测沙眼衣原体crytpic质粒进行测定
公开(公告)号：US6096501A
公开(公告)日：2000-08-01
申请号：US963927
申请日：1997-11-04
申请人： Paul A. Foxall , Dolores M. Berger
发明人： Paul A. Foxall , Dolores M. Berger
IPC分类号： G01N33/569 , C12N15/09 , C12Q1/04 , C12Q1/68 , G01N33/571 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/689
摘要： A region of the Chiamydia trachomatis cryptic plasmid has been identified which is useful for performing amplification assays to determine specifically whether C. trachomiltis is present in the sample being tested. Oligonucleotides useful for performing thermal Strand Displacement Assay (tSDA) reactions on this gene are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for all strains of C. trachomatis and which does not show crossreactivity with the genomes of other microorganisms or with human DNA.
摘要翻译：已经鉴定了沙眼衣原体隐窝质粒的区域，其可用于进行扩增测定以确定是否存在正在测试的样品中的沙眼衣原体。公开了用于对该基因进行热链置换测定（tSDA）反应的寡核苷酸。所公开的寡核苷酸可用于对沙眼衣原体的所有菌株特异性并且不显示与其他微生物的基因组或与人类DNA的交叉反应性的测定。

8. 发明授权

US5427920A Methods and apparatus for detecting biological activities in a specimen 失效
标题翻译：用于检测样本中生物活性的方法和装置
公开(公告)号：US5427920A
公开(公告)日：1995-06-27
申请号：US215957
申请日：1994-03-18
申请人： Klaus W. Berndt , Paul G. Gladnick , Dolores M. Berger
发明人： Klaus W. Berndt , Paul G. Gladnick , Dolores M. Berger
IPC分类号： C12M1/34 , C12Q1/02 , G01N21/49 , G01N21/59 , C12Q1/04 , G01N21/00 , G01N33/48
CPC分类号： G01N21/253 , C12M41/36 , G01N21/51 , G01N2021/4709 , G01N2201/062 , G01N2201/0833 , Y10S435/808
摘要： Methods and apparatus for detecting biological activities in a specimen such as blood are disclosed. The present invention provides a system whereby the intensity of light with a wavelength within the range of about 600 nm to 800 nm introduced into a sample at a first location is measured as it reemerges at a second location. A significant change in the intensity of the reemerging light indicates the presence of biological activity. Preferably, the intensity is also measured at a fourth location, or alternatively, a second light source is disposed at a third location to permit comparative intensity data to be collected. These data are useful in partially identifying the types of microorganisms present in the sample. In preferred embodiments, light emitting diodes are used as the light sources and multiplexed to a plurality of samples. These signals are either detected using photodetectors and demultiplexed, or are collected using fiber-optic light guides and fed to a photomultiplier tube which generates an intensity signal. In either embodiment, the intensity signal is preferably amplified, digitized and stored in a computer.
摘要翻译：公开了用于检测血液样本中生物活性的方法和装置。本发明提供了一种系统，其中当在第二位置重新出射时，测量在第一位置处引入样品中的波长在约600nm至800nm范围内的光的强度。再生光强度的显着变化表明存在生物活性。优选地，还在第四位置处测量强度，或者替代地，第二光源设置在第三位置以允许收集比较强度数据。这些数据可用于部分鉴定样品中存在的微生物的类型。在优选实施例中，使用发光二极管作为光源并且复用到多个样品。这些信号是使用光电检测器进行检测和解复用的，或者使用光纤光导进行采集，并被馈送到产生强度信号的光电倍增管。在任一实施例中，强度信号优选地被放大，数字化并存储在计算机中。

9. 发明授权

US4994378A Method for reducing blood carbon dioxide background in bacterial media by the addition of micelles of saponin and a phospholipid 失效
标题翻译：通过加入皂角苷和磷脂的胶束来减少细菌培养基中血二氧化碳背景的方法
公开(公告)号：US4994378A
公开(公告)日：1991-02-19
申请号：US404475
申请日：1989-09-08
申请人： Dolores M. Berger , Paul E. Goldenbaum , Gregory Tice
发明人： Dolores M. Berger , Paul E. Goldenbaum , Gregory Tice
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04
摘要： A non-ionic lytic agent, preferably saponin for reducing the background carbon dioxide produced by blood cell metabolism is used in the testing of cultures for the presence of microorganisms. The hemolytic agent saponin is combined with a phospholipid, preferably L-.alpha.-Lecithin (phosphatidylcholine), to form mixed micelles which protect saponin from the effects of heat sterilization and high blood cholesterol levels, thus maintaining the lytic activity of saponin. The phospholipid/saponin mixed micelles are added to non-radiometric culture media vials such as Bactec.RTM. NR6A, NR7A and NR8A. The media vials are used in the Bactec.RTM. NR-660 and NR-730 instruments. However, the present invention may also be used in radiometric media such as Bactec.RTM. culture vials 6, 7 and 8 for reducing background carbon dioxide levels detected in C.sup.14 radiometric instruments such as the Bactec.RTM.-460, or in other instruments designed to detect bacteria in the presence of mammalian cells by monitoring the increase in CO.sub.2 produced by the metabolizing bacteria.
摘要翻译：用于减少血液细胞代谢产生的背景二氧化碳的非离子溶解剂，优选皂苷用于微生物存在的培养物的测试中。溶血剂皂苷与磷脂，优选L-α-卵磷脂（磷脂酰胆碱）组合形成混合的胶束，其保护皂苷免受热灭菌和高血胆固醇水平的影响，从而保持皂角苷的溶解活性。将磷脂/皂角苷混合胶束加入非辐射培养基小瓶如Bactec TM NR6A，NR7A和NR8A。介质小瓶用于Bactec TM NR-660和NR-730仪器。然而，本发明也可以用于辐射介质如Bactec TM培养瓶6,7和8中，用于降低C14辐射仪器如Bactec TM -460中检测到的背景二氧化碳水平，或用于其他设计用于检测细菌的仪器在哺乳动物细胞的存在下，通过监测由代谢细菌产生的CO 2的增加。

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