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1. 发明申请

US20110244457A1 IMMUNO-AMPLIFICATION 有权
标题翻译：免疫放大
公开(公告)号：US20110244457A1
公开(公告)日：2011-10-06
申请号：US13072314
申请日：2011-03-25
申请人： James Nadeau , Tobin Hellyer , Dolores M. Berger , William Nussbaumer , Robert Rosenstein , Andrew Kuhn , Sha Sha Wang , Keith Thornton
发明人： James Nadeau , Tobin Hellyer , Dolores M. Berger , William Nussbaumer , Robert Rosenstein , Andrew Kuhn , Sha Sha Wang , Keith Thornton
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6804 , C12Q1/6851
摘要： A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte.
摘要翻译：提供了高灵敏度，低背景的免疫扩增测定法，其提供流线型工作流程，适用于临床相关样品如血液和其他体液的高通量测定。该测定包括使用两个邻近成员，每个邻近成员包含与寡核苷酸缀合的分析物特异性结合成分。结合分析物使邻近成员的寡核苷酸部分充分紧密接触，使寡核苷酸形成扩增子。然后通过扩增扩增子和扩增核酸的检测来检测分析物的存在。通过防止与分析物不复合的邻近成员形成假的或非特异性的扩增子来提高本发明的测定的灵敏度。

2. 发明授权

US07932060B2 Immuno-amplification 有权
标题翻译：免疫扩增
公开(公告)号：US07932060B2
公开(公告)日：2011-04-26
申请号：US10826654
申请日：2004-04-19
申请人： James Nadeau , Tobin Hellyer , Dolores Berger , William Nussbaumer , Robert Rosenstein , Andrew Kuhn , Sha Sha Wang , Keith Thornton
发明人： James Nadeau , Tobin Hellyer , Dolores Berger , William Nussbaumer , Robert Rosenstein , Andrew Kuhn , Sha Sha Wang , Keith Thornton
IPC分类号： C12P19/34 , C12Q1/68 , G01N33/53 , C07H21/04 , C07K1/00
CPC分类号： C12Q1/6804 , C12Q1/6851
摘要： A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte.
摘要翻译：提供了高灵敏度，低背景的免疫扩增测定法，其提供流线型工作流程，适用于临床相关样品如血液和其他体液的高通量测定。该测定包括使用两个邻近成员，每个邻近成员包含与寡核苷酸缀合的分析物特异性结合成分。结合分析物使邻近成员的寡核苷酸部分充分紧密接触，使寡核苷酸形成扩增子。然后通过扩增扩增子和扩增核酸的检测来检测分析物的存在。通过防止与分析物不复合的邻近成员形成假的或非特异性的扩增子来提高本发明的测定的灵敏度。

3. 发明申请

US20050009050A1 Immuno-amplification 有权
标题翻译：免疫扩增
公开(公告)号：US20050009050A1
公开(公告)日：2005-01-13
申请号：US10826654
申请日：2004-04-19
申请人： James Nadeau , Tobin Hellyer , Dolores Berger , William Nussbaumer , Robert Rosenstein , Andrew Kuhn , Sha Wang , Keith Thornton
发明人： James Nadeau , Tobin Hellyer , Dolores Berger , William Nussbaumer , Robert Rosenstein , Andrew Kuhn , Sha Wang , Keith Thornton
IPC分类号： C07K20060101 , C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6804 , C12Q1/6851
摘要： A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte. In one embodiment, target-independent amplicon formation is prevented by using hybridization blocker oligonucleotides that bind oligonucleotide moieties that are not hybridized to each other. Background is further reduced by providing a solid phase capture oligonucleotide that prevents amplicon formation until the captured complex is released.
摘要翻译：提供了高灵敏度，低背景的免疫扩增测定法，其提供流线型工作流程，适用于临床相关样品如血液和其他体液的高通量测定。该测定包括使用两个邻近成员，每个邻近成员包含与寡核苷酸缀合的分析物特异性结合成分。结合分析物使邻近成员的寡核苷酸部分充分紧密接触，使寡核苷酸形成扩增子。然后通过扩增扩增子和扩增核酸的检测来检测分析物的存在。通过防止与分析物不复合的邻近成员形成假的或非特异性的扩增子来提高本发明的测定的灵敏度。在一个实施方案中，通过使用结合彼此不杂交的寡核苷酸部分的杂交阻断剂寡核苷酸来防止靶标无关扩增子形成。通过提供一种固相捕获寡核苷酸来进一步减少背景，所述寡核苷酸防止扩增子形成，直到所捕获的复合物被释放。

4. 发明申请

US20060246495A1 Diagnosis of sepsis 有权
标题翻译：败血症诊断
公开(公告)号：US20060246495A1
公开(公告)日：2006-11-02
申请号：US11404744
申请日：2006-04-14
申请人： James Garrett , Sha-Sha Wang , Keith Thornton , Richard Moore , William Keating , William Nussbaumer , Craig Whiteford
发明人： James Garrett , Sha-Sha Wang , Keith Thornton , Richard Moore , William Keating , William Nussbaumer , Craig Whiteford
IPC分类号： C12Q1/68 , G01N33/567 , G01N33/53
CPC分类号： C12Q1/6883 , C12Q1/6888 , C12Q2600/112 , C12Q2600/158 , C12Q2600/16 , G01N33/56911 , G01N33/6893 , G01N2333/5412 , G01N2333/5421 , G01N2800/26 , G01N2800/50
摘要： Methods for predicting the development of sepsis in a subject at risk for developing sepsis are provided. In one method, features in a biomarker profile of the subject are evaluated. The subject is likely to develop sepsis if these features satisfy a particular value set. Methods for predicting the development of a stage of sepsis in a subject at risk for developing a stage of sepsis are provided. In one method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to have the stage of sepsis if these feature values satisfy a particular value set. Methods of diagnosing sepsis in a subject are provided. In one such method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to develop sepsis when the plurality of features satisfies a particular value set.
摘要翻译：提供了预防脓毒症风险发生的患者脓毒症发展的方法。在一种方法中，评估受试者的生物标志物特征中的特征。如果这些特征满足特定的值集合，主体可能会发展为败血症。提供了预防脓毒症发展阶段脓毒症发展阶段发展的方法。在一种方法中，评估受试者的生物标志物轮廓中的多个特征。如果这些特征值满足特定的值集合，受试者可能具有脓毒症的阶段。提供诊断受试者败血症的方法。在一种这样的方法中，评估对象的生物标志物轮廓中的多个特征。当多个特征满足特定值集合时，受试者可能发展为败血症。

5. 发明申请

US20110105350A1 DIAGNOSIS OF SEPSIS 审中-公开
标题翻译： SEPSIS诊断
公开(公告)号：US20110105350A1
公开(公告)日：2011-05-05
申请号：US12776245
申请日：2010-05-07
申请人： James A. Garrett , Sha-Sha Wang , Keith Thornton , Richard L. Moore , William Keating , William A. Nussbaumer , Craig C. Whiteford
发明人： James A. Garrett , Sha-Sha Wang , Keith Thornton , Richard L. Moore , William Keating , William A. Nussbaumer , Craig C. Whiteford
IPC分类号： C40B30/04 , C12Q1/68
CPC分类号： C12Q1/6883 , C12Q1/6888 , C12Q2600/112 , C12Q2600/158 , C12Q2600/16 , G01N33/56911 , G01N33/6893 , G01N2333/5412 , G01N2333/5421 , G01N2800/26 , G01N2800/50
摘要： Methods for predicting the development of sepsis in a subject at risk for developing sepsis are provided. In one method, features in a biomarker profile of the subject are evaluated. The subject is likely to develop sepsis if these features satisfy a particular value set. Methods for predicting the development of a stage of sepsis in a subject at risk for developing a stage of sepsis are provided. In one method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to have the stage of sepsis if these feature values satisfy a particular value set. Methods of diagnosing sepsis in a subject are provided. In one such method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to develop sepsis when the plurality of features satisfies a particular value set.
摘要翻译：提供了预防脓毒症风险发生的患者脓毒症发展的方法。在一种方法中，评估受试者的生物标志物特征中的特征。如果这些特征满足特定的值集合，主体可能会发展为败血症。提供了预防脓毒症发展阶段脓毒症发展阶段发展的方法。在一种方法中，评估受试者的生物标志物轮廓中的多个特征。如果这些特征值满足特定的值集合，受试者可能具有脓毒症的阶段。提供诊断受试者败血症的方法。在一种这样的方法中，评估对象的生物标志物轮廓中的多个特征。当多个特征满足特定值集合时，受试者可能发展为败血症。

6. 发明申请

US20090246792A1 METHODS FOR DETECTING NUCLEIC ACID SEQUENCE VARIATIONS 有权
标题翻译：检测核酸序列变异的方法
公开(公告)号：US20090246792A1
公开(公告)日：2009-10-01
申请号：US12419737
申请日：2009-04-07
申请人： Sha-Sha WANG , Keith Thornton , James G. Nadeau , Tobin J. Hellyer
发明人： Sha-Sha WANG , Keith Thornton , James G. Nadeau , Tobin J. Hellyer
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2565/1025 , C12Q2535/125 , C12Q2531/119 , C12Q2531/101
摘要： The invention employs an unlabeled signal primer comprising a 5′ adapter sequence for detection of variations in nucleic acid target sequences. The detection system further comprises a reporter probe, the 3′ end of which hybridizes to the complement of the 5′ adapter sequence of the signal primer to produce a 5′ overhang. Polymerase is used to fill in the overhang and synthesize the complement of the 5′ overhang of the reporter probe. Synthesis of the reporter probe complement is detected, either directly or indirectly, as an indication of the presence of the target.
摘要翻译：本发明采用包含5'衔接子序列的未标记信号引物来检测核酸靶序列的变异。检测系统还包括报道探针，其3'末端与信号引物的5'衔接子序列的互补体杂交以产生5'突出端。聚合酶用于填充突出端并合成报告基因探针的5'突出端的互补体。直接或间接检测报道探针补体的合成，作为目标存在的指示。

7. 发明授权

US08323929B2 Methods for detecting nucleic acid sequence variations 有权
标题翻译：检测核酸序列变异的方法
公开(公告)号：US08323929B2
公开(公告)日：2012-12-04
申请号：US12419737
申请日：2009-04-07
申请人： Sha-Sha Wang , Keith Thornton , James G. Nadeau , Tobin J. Hellyer
发明人： Sha-Sha Wang , Keith Thornton , James G. Nadeau , Tobin J. Hellyer
IPC分类号： C12P19/34 , C12Q1/68 , C07H21/02 , C07H21/04 , C07H21/00
CPC分类号： C12Q1/6827 , C12Q2565/1025 , C12Q2535/125 , C12Q2531/119 , C12Q2531/101
摘要： The invention employs an unlabeled signal primer comprising a 5′ adapter sequence for detection of variations in nucleic acid target sequences. The detection system further comprises a reporter probe, the 3′ end of which hybridizes to the complement of the 5′ adapter sequence of the signal primer to produce a 5′ overhang. Polymerase is used to fill in the overhang and synthesize the complement of the 5′ overhang of the reporter probe. Synthesis of the reporter probe complement is detected, either directly or indirectly, as an indication of the presence of the target.
摘要翻译：本发明采用包含5'衔接子序列的未标记信号引物来检测核酸靶序列的变异。检测系统还包括报道探针，其3'末端与信号引物的5'衔接子序列的互补体杂交以产生5'突出端。聚合酶用于填充突出端并合成报告基因探针的5'突出端的互补体。直接或间接检测报道探针补体的合成，作为目标存在的指示。

8. 发明授权

US07767395B2 Diagnosis of sepsis 有权
标题翻译：败血症诊断
公开(公告)号：US07767395B2
公开(公告)日：2010-08-03
申请号：US11404744
申请日：2006-04-14
申请人： James A. Garrett , Sha-Sha Wang , Keith Thornton , Richard L. Moore , William Keating , William A. Nussbaumer , Craig C. Whiteford
发明人： James A. Garrett , Sha-Sha Wang , Keith Thornton , Richard L. Moore , William Keating , William A. Nussbaumer , Craig C. Whiteford
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6883 , C12Q1/6888 , C12Q2600/112 , C12Q2600/158 , C12Q2600/16 , G01N33/56911 , G01N33/6893 , G01N2333/5412 , G01N2333/5421 , G01N2800/26 , G01N2800/50
摘要： Methods for predicting the development of sepsis in a subject at risk for developing sepsis are provided. In one method, features in a biomarker profile of the subject are evaluated. The subject is likely to develop sepsis if these features satisfy a particular value set. Methods for predicting the development of a stage of sepsis in a subject at risk for developing a stage of sepsis are provided. In one method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to have the stage of sepsis if these feature values satisfy a particular value set. Methods of diagnosing sepsis in a subject are provided. In one such method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to develop sepsis when the plurality of features satisfies a particular value set.
摘要翻译：提供了预防脓毒症风险发生的患者脓毒症发展的方法。在一种方法中，评估受试者的生物标志物特征中的特征。如果这些特征满足特定的值集合，主体可能会发展为败血症。提供了预防脓毒症发展阶段脓毒症发展阶段发展的方法。在一种方法中，评估受试者的生物标志物轮廓中的多个特征。如果这些特征值满足特定的值集合，受试者可能具有脓毒症的阶段。提供诊断受试者败血症的方法。在一种这样的方法中，评估对象的生物标志物轮廓中的多个特征。当多个特征满足特定值集合时，受试者可能发展为败血症。

9. 发明授权

US08662667B2 Ophthalmologic visualization system 有权
标题翻译：眼科可视化系统
公开(公告)号：US08662667B2
公开(公告)日：2014-03-04
申请号：US12801689
申请日：2010-06-21
申请人： Thomas Schuhrke , Günter Meckes , Stefan Gräber , Keith Thornton
发明人： Thomas Schuhrke , Günter Meckes , Stefan Gräber , Keith Thornton
IPC分类号： A61B3/14
CPC分类号： A61B3/11 , A61B3/111 , A61B3/112 , A61B3/14 , G06K9/00604
摘要： A method determines the position and/or radius of the limbus and/or the position and/or radius of the pupil of a patient eye. In the method, an image of the patient eye is obtained and a plurality of different ring-shaped comparison objects having respective radii and respective centers are provided. The image is correlated with the plurality of comparison objects to yield a local best match between the image and the comparison objects when there is a coincidence of one of the ring-shaped comparison objects and a ring-shaped jump in brightness in the image having the same radius and the same center. The comparison objects having a local best match with the image are determined. Thereafter, the position of the center of the comparison object having a local best match with the image is selected as the position of the center of the limbus and/or the position of the center of the pupil.
摘要翻译：方法确定角膜缘的位置和/或半径和/或患者眼睛的瞳孔的位置和/或半径。在该方法中，获得患者眼睛的图像，并且提供具有各自的半径和各个中心的多个不同的环形比较对象。该图像与多个比较对象相关联，以便当存在一个环形比较对象之间的一个并且在具有该形状的比较对象的图像中的亮度的环形跳跃时，在图像和比较对象之间产生局部最佳匹配相同的半径和相同的中心。确定与图像具有局部最佳匹配的比较对象。此后，选择与图像具有局部最佳匹配的比较对象的中心的位置作为角膜缘中心的位置和/或瞳孔中心的位置。

10. 发明授权

US08308298B2 Microscopy system for eye surgery 有权
标题翻译：眼科手术系统
公开(公告)号：US08308298B2
公开(公告)日：2012-11-13
申请号：US12801780
申请日：2010-06-24
申请人： Thomas Schuhrke , Günter Meckes , Stefan Gräber , Keith Thornton
发明人： Thomas Schuhrke , Günter Meckes , Stefan Gräber , Keith Thornton
IPC分类号： A61B3/14 , A61B3/00
CPC分类号： A61B3/111 , A61B3/13 , G02B21/0012 , G06T7/73 , G06T2207/10056 , G06T2207/30041
摘要： The invention relates to an eye surgery microscopy system (1) having an imaging optic (14, 11) for the generation of the image of an object plane (15) and having an electronic image sensor (22), which detects the image of the object plane (15) and is connected to a computer unit (5) for the computation of the position of the center of a circular structure (44) of a patient eye (16). The computer unit (5) is designed for the computation of the position of a patient eye (16) outside of the center (52) of the circular structure (44) and provided with at least one marking (46, 48). The computer unit (5) determines the position of the at least one marking (46, 48) with reference to the computed center (52) by means of image processing via correlation with a comparison information, and an angular position of the at least one marking (46, 48) with reference to the computed center (52) by means of image processing.
摘要翻译：本发明涉及一种具有用于产生物体平面（15）的图像并具有电子图像传感器（22）的成像光学元件（14,11）的眼科手术显微镜系统（1），该电子图像传感器检测物体平面（15）并且连接到计算机单元（5）以用于计算患者眼睛（16）的圆形结构（44）的中心的位置。计算机单元（5）被设计用于计算患者眼睛（16）在圆形结构（44）的中心（52）外部的位置并且设置有至少一个标记（46,48）。计算机单元（5）通过与比较信息的相关性的图像处理和参考所计算的中心（52）的至少一个角度位置来确定至少一个标记（46,48）的位置通过图像处理参考计算出的中心（52）来标记（46,48）。

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