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1. 发明申请

US20100041100A1 FAMILY 6 CELLULASE WITH DECREASED INACTIVATION BY LIGNIN 失效
标题翻译：家族6细胞因子被LIGNIN减少灭活
公开(公告)号：US20100041100A1
公开(公告)日：2010-02-18
申请号：US12512403
申请日：2009-07-30
申请人： James A. Lavigne , Brian R. Scott , Martine Whissel , John J. Tomashek
发明人： James A. Lavigne , Brian R. Scott , Martine Whissel , John J. Tomashek
IPC分类号： C12P21/06 , C12N9/14 , C12N15/63 , C12N1/00 , C12P19/04
CPC分类号： C12N9/2437
摘要： A modified Trichoderma reesei Family 6 (TrCel6A) cellulase enzyme comprising amino acid substitutions at one or more positions selected from the group consisting of 129, 322, 363 and 410 of SEQ ID NO: 1 is provided. Genetic constructs and genetically modified microbes comprising nucleic sequences encoding the modified TrCel6a cellulase are also provided. The modified TrCel6A cellulase of the invention display at least a 15% decrease in inactivation by lignin relative to a parental TrCel6A cellulase from which the modified TrCel6A is derived. Such cellulases find use in a variety of applications in industry requiring enzymatic hydrolysis of cellulose in the presence of lignin, e.g., the hydrolysis of pretreated lignocellulosic feedstocks for the production of fermentable sugars, sugar alcohols and fuel alcohols.
摘要翻译：提供了修饰的里氏木霉家族6（TrCel6A）纤维素酶，其在选自SEQ ID NO：1的129,322,363和410的一个或多个位置上包含氨基酸取代。还提供了包含编码修饰的TrCel6a纤维素酶的核酸序列的遗传构建体和遗传修饰微生物。本发明的经修饰的TrCel6A纤维素酶相对于来自其的TrCel6A衍生的亲本TrCel6A纤维素酶显示出至少15％的木质素灭活降低。这种纤维素酶可用于需要在木质素存在下酶促水解纤维素的工业中的各种应用，例如用于生产可发酵糖，糖醇和燃料醇的预处理的木质纤维素原料的水解。

2. 发明授权

US08110389B2 Family 6 cellulase with decreased inactivation by lignin 失效
标题翻译：家族6纤维素酶与木质素的失活减少
公开(公告)号：US08110389B2
公开(公告)日：2012-02-07
申请号：US12512403
申请日：2009-07-30
申请人： James A. Lavigne , Brian R. Scott , Martine Whissel , John J. Tomashek
发明人： James A. Lavigne , Brian R. Scott , Martine Whissel , John J. Tomashek
IPC分类号： C12N9/42 , C12N9/14 , C12N15/00 , C12N1/20 , C12N1/00 , C12P9/04 , C12P21/06 , C12P19/34 , C02F3/34 , D21C1/00 , C07H21/04
CPC分类号： C12N9/2437
摘要： A modified Trichoderma reesei Family 6 (TrCel6A) cellulase enzyme comprising amino acid substitutions at one or more positions selected from the group consisting of 129, 322, 363 and 410 of SEQ ID NO: 1 is provided. Genetic constructs and genetically modified microbes comprising nucleic sequences encoding the modified TrCel6a cellulase are also provided. The modified TrCel6A cellulase of the invention display at least a 15% decrease in inactivation by lignin relative to a parental TrCel6A cellulase from which the modified TrCel6A is derived. Such cellulases find use in a variety of applications in industry requiring enzymatic hydrolysis of cellulose in the presence of lignin, e.g., the hydrolysis of pretreated lignocellulosic feedstocks for the production of fermentable sugars, sugar alcohols and fuel alcohols.
摘要翻译：提供了修饰的里氏木霉家族6（TrCel6A）纤维素酶，其在选自SEQ ID NO：1的129,322,363和410的一个或多个位置上包含氨基酸取代。还提供了包含编码修饰的TrCel6a纤维素酶的核酸序列的遗传构建体和遗传修饰微生物。本发明的经修饰的TrCel6A纤维素酶相对于来自其的TrCel6A衍生的亲本TrCel6A纤维素酶显示出至少15％的木质素灭活降低。这种纤维素酶可用于需要在木质素存在下酶促水解纤维素的工业中的各种应用，例如用于生产可发酵糖，糖醇和燃料醇的预处理的木质纤维素原料的水解。

3. 发明申请

US20090186381A1 CELLULASE VARIANTS WITH REDUCED INHIBITION BY GLUCOSE 有权
标题翻译：由葡萄糖减少抑制的纤维素酶变体
公开(公告)号：US20090186381A1
公开(公告)日：2009-07-23
申请号：US12355373
申请日：2009-01-16
申请人： James A. Lavigne , Christopher M.D. Hill , Annie Tremblay , Patrick St-Pierre , John J. Tomashek
发明人： James A. Lavigne , Christopher M.D. Hill , Annie Tremblay , Patrick St-Pierre , John J. Tomashek
IPC分类号： C12P21/06 , C12P19/00 , C12N9/42 , C12N1/00 , C12N1/19 , C12N1/15 , C12N15/63
CPC分类号： C12N9/2437 , C12P7/10 , C12P19/14 , Y02E50/16
摘要： A Family 6 cellulase variant enzyme comprising one or more than one amino acid substitution selected from a basic, polar or non-polar amino acid at position 103, a valine or isoleucine at position 136, a tyrosine at position 186, a glutamic acid or glutamine at position 365 and a glutamine at position 410 is provided (said position determined form alignment of the parental Family 6 with SEQ ID NO: 1). Genetic constructs and genetically modified microbes comprising DNA sequences encoding the Family 6 cellulase variant are also provided. Family 6 cellulases of the invention display reduced inhibition by glucose relative to the parent Family 6 cellulases. Such cellulases find use in a variety of applications in industry, e.g., in the hydrolysis of pretreated lignocellulosic feedstock, that require cellulose activity in the presence glucose concentrations that would otherwise inhibit the activity of the parental enzyme.
摘要翻译：包含一个或多于一个氨基酸取代的家族6纤维素酶变体酶，其选自103位的碱性，极性或非极性氨基酸，136位的缬氨酸或异亮氨酸，第186位的酪氨酸，谷氨酸或谷氨酰胺在位置365处提供位置410处的谷氨酰胺（所述位置由亲本家族6与SEQ ID NO：1的比对确定）。还提供了包含编码6族纤维素酶变体的DNA序列的遗传构建体和遗传修饰微生物。本发明的家族6纤维素酶相对于亲本家族6纤维素酶显示出降低的葡萄糖抑制。这种纤维素酶可用于工业中的各种应用，例如在预处理的木质纤维素原料的水解中，其在需要纤维素活性的情况下存在葡萄糖浓度，否则其将抑制亲本酶的活性。

4. 发明申请

US20130052694A1 NOVEL CELLOBIOHYDROLASE ENZYMES 有权
标题翻译：新型碳水化合物酶
公开(公告)号：US20130052694A1
公开(公告)日：2013-02-28
申请号：US13593735
申请日：2012-08-24
申请人： Jacqueline Montalibet , Loreta Gudynaite-Savitch , Christopher Hill , Christopher D. Hindle , James A. Lavigne , Nabil Masri , Fuad Tahna , John J. Tomashek
发明人： Jacqueline Montalibet , Loreta Gudynaite-Savitch , Christopher Hill , Christopher D. Hindle , James A. Lavigne , Nabil Masri , Fuad Tahna , John J. Tomashek
IPC分类号： C12N9/42 , C12P19/14 , C12N1/19 , C12N1/21 , C12N15/63 , C12N1/15
CPC分类号： C12Y302/01091 , C12N9/2437 , C12N15/80 , C12P19/14
摘要： Provided are isolated cellobiohydrolases comprising a modified Glycoside Hydrolase (GH) Family 7 catalytic domain, a GH Family 7 catalytic domain and a modified Family 1 carbohydrate binding module (CBM), or both a modified Family 7 catalytic domain and a modified Family 1 CBM. Such isolated cellobiohydrolases exhibit from 45% to about 99.9% amino acid sequence identity to amino acids 1-436 of SEQ ID NO: 1 or to amino acids 1-438 of SEQ ID NO: 2 and improved activity on process substrates. Also provided are genetic constructs and genetically modified microbes for expressing the isolated cellobiohydrolases, a process for producing the isolated cellobiohydrolases, cellulase enzyme mixtures comprising the isolated cellobiohydrolase and a process for hydrolysing a cellulosic substrate with such cellulase enzyme mixtures.
摘要翻译：提供的是分离的纤维二糖水解酶，其包含修饰的糖苷水解酶（GH）家族7催化结构域，GH家族7催化结构域和修饰的家族1碳水化合物结合模块（CBM），或修饰的家族7催化结构域和修饰的家族1 CBM两者。这种分离的纤维二糖水解酶显示与SEQ ID NO：1的氨基酸1-436或SEQ ID NO：2的氨基酸1-438具有45％至约99.9％的氨基酸序列同一性，并且改善了对工艺底物的活性。还提供用于表达分离的纤维二糖水解酶的遗传构建体和遗传修饰的微生物，用于产生分离的纤维二糖水解酶的方法，包含分离的纤维二糖水解酶的纤维素酶混合物和用这种纤维素酶混合物水解纤维素底物的方法。

5. 发明授权

US09279163B2 Cellobiohydrolase enzymes 有权
标题翻译：纤维二糖水解酶
公开(公告)号：US09279163B2
公开(公告)日：2016-03-08
申请号：US13593735
申请日：2012-08-24
申请人： Jacqueline Montalibet , Loreta Gudynaite-Savitch , Christopher Hill , Christopher D. Hindle , James A. Lavigne , Nabil Masri , Fuad Tahna , John J. Tomashek
发明人： Jacqueline Montalibet , Loreta Gudynaite-Savitch , Christopher Hill , Christopher D. Hindle , James A. Lavigne , Nabil Masri , Fuad Tahna , John J. Tomashek
IPC分类号： C12N9/42 , C12N15/80 , C12P19/14
CPC分类号： C12Y302/01091 , C12N9/2437 , C12N15/80 , C12P19/14
摘要： Provided are isolated cellobiohydrolases comprising a modified Glycoside Hydrolase (GH) Family 7 catalytic domain, a GH Family 7 catalytic domain and a modified Family 1 carbohydrate binding module (CBM), or both a modified Family 7 catalytic domain and a modified Family 1 CBM. Such isolated cellobiohydrolases exhibit from 45% to about 99.9% amino acid sequence identity to amino acids 1-436 of SEQ ID NO: 1 or to amino acids 1-438 of SEQ ID NO: 2 and improved activity on process substrates. Also provided are genetic constructs and genetically modified microbes for expressing the isolated cellobiohydrolases, a process for producing the isolated cellobiohydrolases, cellulase enzyme mixtures comprising the isolated cellobiohydrolase and a process for hydrolyzing a cellulosic substrate with such cellulase enzyme mixtures.
摘要翻译：提供的是分离的纤维二糖水解酶，其包含修饰的糖苷水解酶（GH）家族7催化结构域，GH家族7催化结构域和修饰的家族1碳水化合物结合模块（CBM），或修饰的家族7催化结构域和修饰的家族1 CBM两者。这种分离的纤维二糖水解酶显示与SEQ ID NO：1的氨基酸1-436或SEQ ID NO：2的氨基酸1-438具有45％至约99.9％的氨基酸序列同一性，并且改善了对工艺底物的活性。还提供了用于表达分离的纤维二糖水解酶的遗传构建体和遗传修饰的微生物，用于产生分离的纤维二糖水解酶的方法，包含分离的纤维二糖水解酶的纤维素酶混合物和用这种纤维素酶混合物水解纤维素底物的方法。

6. 发明授权

US08012734B2 Cellulase variants with reduced inhibition by glucose 有权
标题翻译：纤维素酶变体具有降低的葡萄糖抑制作用
公开(公告)号：US08012734B2
公开(公告)日：2011-09-06
申请号：US12355373
申请日：2009-01-16
申请人： James A. Lavigne , Christopher M. D. Hill , Annie Tremblay , Patrick St-Pierre , John J. Tomashek
发明人： James A. Lavigne , Christopher M. D. Hill , Annie Tremblay , Patrick St-Pierre , John J. Tomashek
IPC分类号： C12P21/06 , C12N9/02 , C12N9/42 , C12N1/20 , C07H21/04
CPC分类号： C12N9/2437 , C12P7/10 , C12P19/14 , Y02E50/16
摘要： A Family 6 cellulase variant enzyme comprising one or more than one amino acid substitution selected from a basic, polar or non-polar amino acid at position 103, a valine or isoleucine at position 136, a tyrosine at position 186, a glutamic acid or glutamine at position 365 and a glutamine at position 410 is provided (said position determined form alignment of the parental Family 6 with SEQ ID NO: 1). Genetic constructs and genetically modified microbes comprising DNA sequences encoding the Family 6 cellulase variant are also provided. Family 6 cellulases of the invention display reduced inhibition by glucose relative to the parent Family 6 cellulases. Such cellulases find use in a variety of applications in industry, e.g., in the hydrolysis of pretreated lignocellulosic feedstock, that require cellulose activity in the presence glucose concentrations that would otherwise inhibit the activity of the parental enzyme.
摘要翻译：包含一个或多于一个氨基酸取代的家族6纤维素酶变体酶，其选自103位的碱性，极性或非极性氨基酸，136位的缬氨酸或异亮氨酸，第186位的酪氨酸，谷氨酸或谷氨酰胺在位置365处提供位置410处的谷氨酰胺（所述位置由亲本家族6与SEQ ID NO：1的比对确定）。还提供了包含编码6族纤维素酶变体的DNA序列的遗传构建体和遗传修饰微生物。本发明的家族6纤维素酶相对于亲本家族6纤维素酶显示出降低的葡萄糖抑制。这种纤维素酶可用于工业中的各种应用，例如在预处理的木质纤维素原料的水解中，其在需要纤维素活性的情况下存在葡萄糖浓度，否则其将抑制亲本酶的活性。

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