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1. 发明申请

US20090186381A1 CELLULASE VARIANTS WITH REDUCED INHIBITION BY GLUCOSE 有权
标题翻译：由葡萄糖减少抑制的纤维素酶变体
公开(公告)号：US20090186381A1
公开(公告)日：2009-07-23
申请号：US12355373
申请日：2009-01-16
申请人： James A. Lavigne , Christopher M.D. Hill , Annie Tremblay , Patrick St-Pierre , John J. Tomashek
发明人： James A. Lavigne , Christopher M.D. Hill , Annie Tremblay , Patrick St-Pierre , John J. Tomashek
IPC分类号： C12P21/06 , C12P19/00 , C12N9/42 , C12N1/00 , C12N1/19 , C12N1/15 , C12N15/63
CPC分类号： C12N9/2437 , C12P7/10 , C12P19/14 , Y02E50/16
摘要： A Family 6 cellulase variant enzyme comprising one or more than one amino acid substitution selected from a basic, polar or non-polar amino acid at position 103, a valine or isoleucine at position 136, a tyrosine at position 186, a glutamic acid or glutamine at position 365 and a glutamine at position 410 is provided (said position determined form alignment of the parental Family 6 with SEQ ID NO: 1). Genetic constructs and genetically modified microbes comprising DNA sequences encoding the Family 6 cellulase variant are also provided. Family 6 cellulases of the invention display reduced inhibition by glucose relative to the parent Family 6 cellulases. Such cellulases find use in a variety of applications in industry, e.g., in the hydrolysis of pretreated lignocellulosic feedstock, that require cellulose activity in the presence glucose concentrations that would otherwise inhibit the activity of the parental enzyme.
摘要翻译：包含一个或多于一个氨基酸取代的家族6纤维素酶变体酶，其选自103位的碱性，极性或非极性氨基酸，136位的缬氨酸或异亮氨酸，第186位的酪氨酸，谷氨酸或谷氨酰胺在位置365处提供位置410处的谷氨酰胺（所述位置由亲本家族6与SEQ ID NO：1的比对确定）。还提供了包含编码6族纤维素酶变体的DNA序列的遗传构建体和遗传修饰微生物。本发明的家族6纤维素酶相对于亲本家族6纤维素酶显示出降低的葡萄糖抑制。这种纤维素酶可用于工业中的各种应用，例如在预处理的木质纤维素原料的水解中，其在需要纤维素活性的情况下存在葡萄糖浓度，否则其将抑制亲本酶的活性。

2. 发明授权

US08012734B2 Cellulase variants with reduced inhibition by glucose 有权
标题翻译：纤维素酶变体具有降低的葡萄糖抑制作用
公开(公告)号：US08012734B2
公开(公告)日：2011-09-06
申请号：US12355373
申请日：2009-01-16
申请人： James A. Lavigne , Christopher M. D. Hill , Annie Tremblay , Patrick St-Pierre , John J. Tomashek
发明人： James A. Lavigne , Christopher M. D. Hill , Annie Tremblay , Patrick St-Pierre , John J. Tomashek
IPC分类号： C12P21/06 , C12N9/02 , C12N9/42 , C12N1/20 , C07H21/04
CPC分类号： C12N9/2437 , C12P7/10 , C12P19/14 , Y02E50/16
摘要： A Family 6 cellulase variant enzyme comprising one or more than one amino acid substitution selected from a basic, polar or non-polar amino acid at position 103, a valine or isoleucine at position 136, a tyrosine at position 186, a glutamic acid or glutamine at position 365 and a glutamine at position 410 is provided (said position determined form alignment of the parental Family 6 with SEQ ID NO: 1). Genetic constructs and genetically modified microbes comprising DNA sequences encoding the Family 6 cellulase variant are also provided. Family 6 cellulases of the invention display reduced inhibition by glucose relative to the parent Family 6 cellulases. Such cellulases find use in a variety of applications in industry, e.g., in the hydrolysis of pretreated lignocellulosic feedstock, that require cellulose activity in the presence glucose concentrations that would otherwise inhibit the activity of the parental enzyme.
摘要翻译：包含一个或多于一个氨基酸取代的家族6纤维素酶变体酶，其选自103位的碱性，极性或非极性氨基酸，136位的缬氨酸或异亮氨酸，第186位的酪氨酸，谷氨酸或谷氨酰胺在位置365处提供位置410处的谷氨酰胺（所述位置由亲本家族6与SEQ ID NO：1的比对确定）。还提供了包含编码6族纤维素酶变体的DNA序列的遗传构建体和遗传修饰微生物。本发明的家族6纤维素酶相对于亲本家族6纤维素酶显示出降低的葡萄糖抑制。这种纤维素酶可用于工业中的各种应用，例如在预处理的木质纤维素原料的水解中，其在需要纤维素活性的情况下存在葡萄糖浓度，否则其将抑制亲本酶的活性。

3. 发明申请

US20100016570A1 MODIFIED FAMILY 6 GLYCOSIDASES WITH ALTERED SUBSTRATE SPECIFICITY 失效
标题翻译：改良的家禽6型糖尿病具有改变的基质特异性
公开(公告)号：US20100016570A1
公开(公告)日：2010-01-21
申请号：US12504070
申请日：2009-07-16
申请人： John Tomashek , James Lavigne , Annie Tremblay , Patrick St-Pierre
发明人： John Tomashek , James Lavigne , Annie Tremblay , Patrick St-Pierre
IPC分类号： C12N9/24 , C07H21/04 , C12N1/15 , C12N1/18 , C07H1/00
CPC分类号： C12N9/2437 , C12N1/22 , C12N9/244 , C12Y302/01006 , C12Y302/01091 , Y02E50/17
摘要： A modified Family 6 glycosidase enzyme comprising amino acid substitutions at one or more positions selected from the group 182, 367, 399, 400 and 427 is provided (the position determined form alignment of a parental Family 6 glycosidase with SEQ ID NO: 1). Genetic constructs and genetically modified microbes comprising nucleic sequences encoding the modified Family 6 glycosidase are also provided. Family 6 glycosidase of the invention display decreased hydrolysis activity of beta 1-4 linked polysaccharides and increased hydrolysis activity of beta 1-3, 1-4 linked polysaccharides compared with a parental Family 6 glycosidase. Such glycosidases find use in a variety of applications in industry, e.g., in hydrolysis of beta 1-3, 1-4 linked polysaccharides during the processing of cereal grains or the production of alcohol, animal feed or food products.
摘要翻译：提供了修饰的家族6糖苷酶，其包含在选自182,367,399,440和427中的一个或多个位置上的氨基酸取代（通过亲本6族糖苷酶与SEQ ID NO：1的比对确定的位置）。还提供了包含编码修饰的家族6糖苷酶的核酸序列的遗传构建体和遗传修饰微生物。本发明的家族6糖苷酶与亲本6族糖苷酶相比，显示β1-4连接多糖的水解活性降低，β1-3,1-4连接多糖的水解活性增加。这样的糖苷酶可用于工业中的各种应用，例如在谷物加工过程中水解β1-3,1-连接的多糖或生产醇，动物饲料或食品。

4. 发明授权

US08263379B2 Modified family 6 glycosidases with altered substrate specificity 失效
标题翻译：改良的家族6糖苷酶具有改变的底物特异性
公开(公告)号：US08263379B2
公开(公告)日：2012-09-11
申请号：US12504070
申请日：2009-07-16
申请人： John Tomashek , James Lavigne , Annie Tremblay , Patrick St-Pierre
发明人： John Tomashek , James Lavigne , Annie Tremblay , Patrick St-Pierre
IPC分类号： C12N9/24 , C12N1/00 , C12P21/06 , C12P7/06 , C07H21/04 , C07H1/00 , C07K1/00 , C12C11/00 , A21D2/00 , A23B7/00
CPC分类号： C12N9/2437 , C12N1/22 , C12N9/244 , C12Y302/01006 , C12Y302/01091 , Y02E50/17
摘要： A modified Family 6 glycosidase enzyme comprising amino acid substitutions at one or more positions selected from the group 182, 367, 399, 400 and 427 is provided (the position determined form alignment of a parental Family 6 glycosidase with SEQ ID NO: 1). Genetic constructs and genetically modified microbes comprising nucleic sequences encoding the modified Family 6 glycosidase are also provided. Family 6 glycosidase of the invention display decreased hydrolysis activity of beta 1-4 linked polysaccharides and increased hydrolysis activity of beta 1-3, 1-4 linked polysaccharides compared with a parental Family 6 glycosidase. Such glycosidases find use in a variety of applications in industry, e.g., in hydrolysis of beta 1-3, 1-4 linked polysaccharides during the processing of cereal grains or the production of alcohol, animal feed or food products.
摘要翻译：提供了修饰的家族6糖苷酶，其包含在选自182,367,399,440和427中的一个或多个位置上的氨基酸取代（通过亲本6族糖苷酶与SEQ ID NO：1的比对确定的位置）。还提供了包含编码修饰的家族6糖苷酶的核酸序列的遗传构建体和遗传修饰微生物。本发明的家族6糖苷酶与亲本6族糖苷酶相比，显示β1-4连接多糖的水解活性降低，β1-3,1-4连接多糖的水解活性增加。这样的糖苷酶可用于工业中的各种应用，例如在谷物加工过程中水解β1-3,1-连接的多糖或生产醇，动物饲料或食品。

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