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1. 发明授权

US5420249A Process for the recovery of .alpha.-lactalbumin and .beta.-lactoglobulin from a whey protein product 失效
标题翻译：从乳清蛋白产物中回收α-乳白蛋白和β-乳球蛋白的方法
公开(公告)号：US5420249A
公开(公告)日：1995-05-30
申请号：US170497
申请日：1993-12-20
申请人： Jacobus N. de Wit , Henricus Bronts
发明人： Jacobus N. de Wit , Henricus Bronts
IPC分类号： A23L1/305 , A23C9/146 , A23J1/20 , A23J3/08 , A61K38/16 , C07K1/14 , C07K14/47 , C07K14/76 , C07K1/00 , A61K35/20
CPC分类号： A23J1/205 , A23C9/146 , A23V2002/00
摘要： This invention relates to a process for the recovery of .alpha.-lactalbumin and/or .beta.-lactoglobulin enriched whey protein concentrate from a whey protein product. The process comprises the following steps:a) incubating a solution comprising whey protein product with a calcium-binding ionic exchange resin in its acid form to initiate the instabilization of .alpha.-lactalbumin,b) adjusting the pH of the treated protein product solution to a value between 4.3 and 4.8, after separation of the resin,c) incubating the protein product solution at a temperature between 10 and 50.degree. C. to promote the flocculation of .alpha.-lactalbumin,d) fractionating the proteins in the protein product solution at pH 4.3-4.8, providing an .alpha.-lactalbumin enriched fraction and a .beta.-lactoglobulin enriched fraction,e) raising the pH of the .alpha.-lactalbumin enriched fraction sufficiently to solubilize the .alpha.-lactalbumin fraction, andf) optionally raising the pH of the .beta.-lactoglobulin enriched fraction sufficiently to neutralize the .beta.-lactoglobulin fraction.
摘要翻译：本发明涉及从乳清蛋白产物中回收富含β-乳球蛋白的乳清蛋白浓缩物的α-乳白蛋白和/或β-乳球蛋白浓缩物的方法。该方法包括以下步骤：a）将包含乳清蛋白产物的溶液与其酸形式的钙结合离子交换树脂一起孵育以引发α-乳白蛋白的不稳定，b）将经处理的蛋白质产物溶液的pH调节至值在4.3和4.8之间，分离树脂后，c）在10至50℃的温度下孵育蛋白质产物溶液以促进α-乳白蛋白的絮凝，d）将蛋白质产物溶液中的蛋白质在pH 4.3-4.8，提供富含α-不饱和白蛋白的部分和富含β-乳球蛋白的部分，e）提高富含α-精氨白蛋白的级分的pH以充分溶解α-乳白蛋白部分，和f）任选地提高β 富含乳球蛋白的级分足以中和β-乳球蛋白部分。

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