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1. 发明申请

US20090032399A1 Methods for Forming Small-Volume Electrical Contacts and Material Manipulations With Fluid Microchannels 有权
标题翻译：用流体微通道形成小体积电触头和材料操作的方法
公开(公告)号：US20090032399A1
公开(公告)日：2009-02-05
申请号：US11875031
申请日：2007-10-19
申请人： Stephen C. Jacobson , J. Michael Ramsey , Christopher T. Culbertson , William B. Whitten , Robert S. Foote
发明人： Stephen C. Jacobson , J. Michael Ramsey , Christopher T. Culbertson , William B. Whitten , Robert S. Foote
IPC分类号： G01N27/447
CPC分类号： B01D57/02 , B01D61/422 , B01D61/427 , B01D61/445 , B01L3/50273 , B01L3/502753 , B01L2200/10 , B01L2300/0816 , B01L2400/0415 , B01L2400/0418 , B01L2400/049 , F04B17/00 , F04B19/006 , G01N27/44704 , G01N27/44791
摘要： A microfabricated device employing a bridging membrane and methods for electrokinetic transport of a liquid phase biological or chemical material using the same are described. The bridging membrane is deployed in or adjacent to a microchannel and permits either ionic current flow or the transport of gas species, while inhibiting the bulk flow of material. The use of bridging membranes in accordance with this invention is applicable to a variety of processes, including electrokinetically induced pressure flow in a region of a microchannel that is not influenced by an electric field, sample concentration enhancement and injection, as well as improving the analysis of materials where it is desired to eliminate electrophoretic bias. Other applications of the bridging membranes according to this invention include the separation of species from a sample material, valving of fluids in a microchannel network, mixing of different materials in a microchannel, and the pumping of fluids.
摘要翻译：描述了使用桥接膜的微制造装置和使用其的液相生物或化学材料的电动转运方法。桥接膜部署在微通道中或邻近微通道，并允许离子电流流动或气体物质的输送，同时抑制材料的大量流动。根据本发明的桥接膜的使用适用于各种工艺，包括不受电场影响的微通道区域中的电动感应压力流动，样品浓度增强和注入，以及改进分析需要消除电泳偏压的材料。根据本发明的桥接膜的其它应用包括物质与样品材料的分离，微通道网络中的流体阀门，微通道中的不同材料的混合以及流体的泵送。

2. 发明申请

US20080272000A1 Methods for Forming Small-Volume Electrical Contacts and Material Manipulations With Fluidic Microchannels 有权
标题翻译：用流体微通道形成小体积电触点和材料操作的方法
公开(公告)号：US20080272000A1
公开(公告)日：2008-11-06
申请号：US11875027
申请日：2007-10-19
申请人： Stephen C. Jacobson , J. Michael Ramsey , Christopher T. Culbertson , William B. Whitten , Robert S. Foote
发明人： Stephen C. Jacobson , J. Michael Ramsey , Christopher T. Culbertson , William B. Whitten , Robert S. Foote
IPC分类号： G01N27/26 , G01N27/00
CPC分类号： B01D57/02 , B01D61/422 , B01D61/427 , B01D61/445 , B01L3/50273 , B01L3/502753 , B01L2200/10 , B01L2300/0816 , B01L2400/0415 , B01L2400/0418 , B01L2400/049 , F04B17/00 , F04B19/006 , G01N27/44704 , G01N27/44791
摘要： A microfabricated device employing a bridging membrane and methods for electrokinetic transport of a liquid phase biological or chemical material using the same are described. The bridging membrane is deployed in or adjacent to a microchannel and permits either ionic current flow or the transport of gas species, while inhibiting the bulk flow of material. The use of bridging membranes in accordance with this invention is applicable to a variety of processes, including electrokinetically induced pressure flow in a region of a microchannel that is not influenced by an electric field, sample concentration enhancement and injection, as well as improving the analysis of materials where it is desired to eliminate electrophoretic bias. Other applications of the bridging membranes according to this invention include the separation of species from a sample material, valving of fluids in a microchannel network, mixing of different materials in a microchannel, and the pumping of fluids.
摘要翻译：描述了使用桥接膜的微制造装置和使用其的液相生物或化学材料的电动转运方法。桥接膜部署在微通道中或邻近微通道，并允许离子电流流动或气体物质的输送，同时抑制材料的大量流动。根据本发明的桥接膜的使用适用于各种工艺，包括不受电场影响的微通道区域中的电动感应压力流动，样品浓度增强和注入，以及改进分析需要消除电泳偏压的材料。根据本发明的桥接膜的其它应用包括物质与样品材料的分离，微通道网络中的流体阀门，微通道中的不同材料的混合以及流体的泵送。

3. 发明授权

US08268633B2 Microfluidic devices for the controlled manipulation of small volumes 有权
标题翻译：微流控器件用于小容量的受控操纵
公开(公告)号：US08268633B2
公开(公告)日：2012-09-18
申请号：US11696785
申请日：2007-04-05
申请人： J. Michael Ramsey , Stephen C. Jacobson
发明人： J. Michael Ramsey , Stephen C. Jacobson
IPC分类号： G01N15/06 , G01N33/00 , G01N33/48
CPC分类号： B01F13/0071 , B01F5/0471 , B01F13/0076 , B01F13/0077 , B01J19/0093 , B01J2219/00853 , B01J2219/00891 , B01L3/502761 , B01L3/502784 , B01L2200/0605 , B01L2200/0647 , B01L2200/0673 , B01L2300/0816 , B01L2300/088 , B01L2400/0415 , B01L2400/049 , F16K99/0001 , F16K99/0017 , F16K99/0019 , F16K99/0042 , F16K99/0051 , F16K99/0059 , F16K2099/0084 , G01N35/08 , G11C19/00 , G11C25/00 , Y10T436/11 , Y10T436/117497 , Y10T436/118339 , Y10T436/2575
摘要： A method for conducting a broad range of biochemical analyses or manipulations on a series of nano- to subnanoliter reaction volumes and an apparatus for carrying out the same are disclosed. The invention is implemented on a fluidic microchip to provide high serial throughput. In particular, the disclosed device is a microfabricated channel device that can manipulate nanoliter or subnanoliter reaction volumes in a controlled manner to produce results at rates of 1 to 10 Hz per channel. The reaction volumes are manipulated in serial fashion analogous to a digital shift register. The invention has application to such problems as screening molecular or cellular targets using single beads from split-synthesis combinatorial libraries, screening single cells for RNA or protein expression, genetic diagnostic screening at the single cell level, or performing single cell signal transduction studies.
摘要翻译：公开了一系列用于进行一系列纳米级亚纳速器反应体积的生化分析或操作的方法和用于进行其的装置。本发明在流体微芯片上实现以提供高的串行吞吐量。特别地，所公开的装置是微加工的通道装置，其可以以受控的方式操纵纳升或亚纳溶药反应体积，以每通道1至10Hz的速率产生结果。反应体积以类似于数字移位寄存器的串行方式被操纵。本发明可应用于使用来自分裂合成组合文库的单个珠筛选分子或细胞靶标，筛选单细胞用于RNA或蛋白质表达，在单细胞水平进行遗传诊断筛选或进行单细胞信号转导研究的问题。

4. 发明授权

US07422669B2 Microfluidic device and method for focusing, segmenting, and dispensing of a fluid stream 有权
标题翻译：用于聚焦，分段和分配流体流的微流体装置和方法
公开(公告)号：US07422669B2
公开(公告)日：2008-09-09
申请号：US10758718
申请日：2004-01-16
申请人： Stephen C. Jacobson , J. Michael Ramsey
发明人： Stephen C. Jacobson , J. Michael Ramsey
IPC分类号： G01N27/447 , G01N27/453 , B01L3/02
CPC分类号： B01F13/0062 , B01F13/0059 , B01F13/0076 , B01L3/5027 , B01L3/502776 , B01L2200/0605 , B01L2200/0636 , B01L2200/0673 , B01L2300/0816 , B01L2400/0415 , B01L2400/0487 , G01N27/44791
摘要： A microfluidic device and method for forming and dispensing minute volume segments of a material are described. In accordance with the present invention, a microfluidic device and method are provided for spatially confining the material in a focusing element. The device is also adapted for segmenting the confined material into minute volume segments, and dispensing a volume segment to a waste or collection channel. The device further includes means for driving the respective streams of sample and focusing fluids through respective channels into a chamber, such that the focusing fluid streams spatially confine the sample material. The device may also include additional means for driving a minute volume segment of the spatially confined sample material into a collection channel in fluid communication with the waste reservoir.
摘要翻译：描述了用于形成和分配材料的微小体积段的微流体装置和方法。根据本发明，提供了一种微流体装置和方法，用于将材料空间上限制在聚焦元件中。该装置还适于将限制的材料分割成微小的体积段，并将体积段分配到废物或收集通道。该装置还包括用于将样品的各个流和通过相应通道聚焦的流体驱动到室中的装置，使得聚焦流体流在空间上限制样品材料。该装置还可以包括用于将空间限制的样品材料的微小体积段驱动到与废物储存器流体连通的收集通道中的附加装置。

5. 发明授权

US07419575B2 Microfluidic systems and methods for transport and lysis of cells and analysis of cell lysate 有权
标题翻译：微流体系统和细胞运输和裂解方法以及细胞裂解液的分析
公开(公告)号：US07419575B2
公开(公告)日：2008-09-02
申请号：US10858096
申请日：2004-06-01
申请人： Christopher T. Culbertson , Stephen C. Jacobson , Maxine A. McClain , J. Michael Ramsey
发明人： Christopher T. Culbertson , Stephen C. Jacobson , Maxine A. McClain , J. Michael Ramsey
IPC分类号： G01N27/453 , C12M1/33 , C12N13/00
CPC分类号： B01L3/502761 , B01L2200/0647 , B01L2400/0415 , B01L2400/0487 , C12N1/066 , C12N13/00 , G01N27/44743
摘要： Microfluidic systems and methods are disclosed which are adapted to transport and lyse cellular components of a test sample for analysis. The disclosed microfluidic systems and methods, which employ an electric field to rupture the cell membrane, cause unusually rapid lysis, thereby minimizing continued cellular activity and resulting in greater accuracy of analysis of cell processes.
摘要翻译：公开了微流体系统和方法，其适于运输和裂解测试样品的细胞组分用于分析。公开的使用电场破裂细胞膜的微流体系统和方法引起非常快速的裂解，从而最小化持续的细胞活性并导致细胞过程分析的更高精度。

6. 发明授权

US07238268B2 Microfluidic devices for the controlled manipulation of small volumes 有权
标题翻译：微流控器件用于小容量的受控操纵
公开(公告)号：US07238268B2
公开(公告)日：2007-07-03
申请号：US10373129
申请日：2003-02-24
申请人： J. Michael Ramsey , Stephen C. Jacobson
发明人： J. Michael Ramsey , Stephen C. Jacobson
IPC分类号： G01N27/447 , G01N27/453 , G01N21/00
CPC分类号： B01F13/0071 , B01F5/0471 , B01F13/0076 , B01F13/0077 , B01J19/0093 , B01J2219/00853 , B01J2219/00891 , B01L3/502761 , B01L3/502784 , B01L2200/0605 , B01L2200/0647 , B01L2200/0673 , B01L2300/0816 , B01L2300/088 , B01L2400/0415 , B01L2400/049 , F16K99/0001 , F16K99/0017 , F16K99/0019 , F16K99/0042 , F16K99/0051 , F16K99/0059 , F16K2099/0084 , G01N35/08 , G11C19/00 , G11C25/00 , Y10T436/11 , Y10T436/117497 , Y10T436/118339 , Y10T436/2575
摘要： A method for conducting a broad range of biochemical analyses or manipulations on a series of nano- to subnanoliter reaction volumes and an apparatus for carrying out the same are disclosed. The invention is implemented on a fluidic microchip to provide high serial throughput. In particular, the disclosed device is a microfabricated channel device that can manipulate nanoliter or subnanoliter reaction volumes in a controlled manner to produce results at rates of 1 to 10 Hz per channel. The reaction volumes are manipulated in serial fashion analogous to a digital shift register. The invention has application to such problems as screening molecular or cellular targets using single beads from split-synthesis combinatorial libraries, screening single cells for RNA or protein expression, genetic diagnostic screening at the single cell level, or performing single cell signal transduction studies.
摘要翻译：公开了一系列用于进行一系列纳米级亚纳速器反应体积的生化分析或操作的方法和用于进行其的装置。本发明在流体微芯片上实现以提供高的串行吞吐量。特别地，所公开的装置是微加工的通道装置，其可以以受控的方式操纵纳升或亚纳溶药反应体积，以每通道1至10Hz的速率产生结果。反应体积以类似于数字移位寄存器的串行方式被操纵。本发明可应用于使用来自分裂合成组合文库的单个珠筛选分子或细胞靶标，筛选单细胞用于RNA或蛋白质表达，在单细胞水平进行遗传诊断筛选或进行单细胞信号转导研究的问题。

7. 发明授权

US06213151B1 Microfluidic circuit designs for performing fluidic manipulations that reduce the number of pumping sources and fluid reservoirs 有权
标题翻译：用于进行流体操作的微流体电路设计，其减少泵送源和流体储存器的数量
公开(公告)号：US06213151B1
公开(公告)日：2001-04-10
申请号：US09557435
申请日：2000-04-25
申请人： Stephen C. Jacobson , J. Michael Ramsey
发明人： Stephen C. Jacobson , J. Michael Ramsey
IPC分类号： G01N2726
CPC分类号： B01L3/50273 , B01L3/502 , B01L3/502746 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487 , B01L2400/084 , G01N1/38 , Y10S366/03 , Y10T137/2076 , Y10T137/2191 , Y10T137/2224
摘要： A microfabricated device and method for proportioning and mixing biological or chemical materials by pressure- or vacuum-driven flow is disclosed. The microfabricated device mixes a plurality of materials in volumetric proportions controlled by the flow resistances of tributary reagent channels through which the materials are transported. The microchip includes two or more tributary reagent channels combining at one or more junctions to form one or more mixing channels. By varying the geometries of the channels (length, cross section, etc.), a plurality of reagent materials can be mixed at a junction such that the proportions of the reagent materials in the mixing channel depend on a ratio of the channel geometries and material properties. Such an approach facilitates flow division on the microchip without relying on techniques external to the microchip. Microchannel designs that provide the necessary flow division to accomplish valving operations using a minimum of pressure or vacuum sources are also described. In addition, microchannel designs that accomplish fluidic operation utilizing a minimal number of fluidic reservoirs are disclosed.
摘要翻译：公开了一种用于通过压力或真空驱动流量来比例混合生物或化学材料的微制造装置和方法。微加工装置以容积比例混合多种材料，所述体积比例由所述材料被输送通过的辅助试剂通道的流动阻力控制。微芯片包括在一个或多个结处组合以形成一个或多个混合通道的两个或更多个支流试剂通道。通过改变通道的几何形状（长度，横截面等），可以在接合处混合多个试剂材料，使得混合通道中的试剂材料的比例取决于通道几何形状和材料的比例属性。这种方法有助于微芯片上的分流，而不依赖于微芯片外部的技术。还描述了使用最小压力或真空源来提供必要的流动分割以完成阀门操作的微通道设计。此外，公开了利用最少数量的流体储存器来完成流体操作的微通道设计。

8. 发明授权

US08083915B2 Methods for forming small-volume electrical contacts and material manipulations with fluid microchannels 有权
标题翻译：用流体微通道形成小容量电触点和材料操作的方法
公开(公告)号：US08083915B2
公开(公告)日：2011-12-27
申请号：US11875031
申请日：2007-10-19
申请人： Stephen C. Jacobson , J. Michael Ramsey , Christopher T. Culbertson , William B. Whitten , Robert S. Foote
发明人： Stephen C. Jacobson , J. Michael Ramsey , Christopher T. Culbertson , William B. Whitten , Robert S. Foote
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： B01D57/02 , B01D61/422 , B01D61/427 , B01D61/445 , B01L3/50273 , B01L3/502753 , B01L2200/10 , B01L2300/0816 , B01L2400/0415 , B01L2400/0418 , B01L2400/049 , F04B17/00 , F04B19/006 , G01N27/44704 , G01N27/44791
摘要： A microfabricated device employing a bridging membrane and methods for electrokinetic transport of a liquid phase biological or chemical material using the same are described. The bridging membrane is deployed in or adjacent to a microchannel and permits either ionic current flow or the transport of gas species, while inhibiting the bulk flow of material. The use of bridging membranes in accordance with this invention is applicable to a variety of processes, including electrokinetically induced pressure flow in a region of a microchannel that is not influenced by an electric field, sample concentration enhancement and injection, as well as improving the analysis of materials where it is desired to eliminate electrophoretic bias. Other applications of the bridging membranes according to this invention include the separation of species from a sample material, valving of fluids in a microchannel network, mixing of different materials in a microchannel, and the pumping of fluids.
摘要翻译：描述了使用桥接膜的微制造装置和使用其的液相生物或化学材料的电动转运方法。桥接膜部署在微通道中或邻近微通道，并允许离子电流流动或气体物质的输送，同时抑制材料的大量流动。根据本发明的桥接膜的使用适用于各种工艺，包括不受电场影响的微通道区域中的电动感应压力流动，样品浓度增强和注入，以及改进分析需要消除电泳偏压的材料。根据本发明的桥接膜的其它应用包括物质与样品材料的分离，微通道网络中的流体阀门，微通道中的不同材料的混合以及流体的泵送。

9. 发明授权

US07931790B2 Methods for forming small-volume electrical contacts and material manipulations with fluidic microchannels 有权
标题翻译：用流体微通道形成小体积电触头和材料操作的方法
公开(公告)号：US07931790B2
公开(公告)日：2011-04-26
申请号：US11875019
申请日：2007-10-19
申请人： Stephen C. Jacobson , J. Michael Ramsey , Christopher T. Culbertson , William B. Whitten , Robert S. Foote
发明人： Stephen C. Jacobson , J. Michael Ramsey , Christopher T. Culbertson , William B. Whitten , Robert S. Foote
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： B01D57/02 , B01D61/422 , B01D61/427 , B01D61/445 , B01L3/50273 , B01L3/502753 , B01L2200/10 , B01L2300/0816 , B01L2400/0415 , B01L2400/0418 , B01L2400/049 , F04B17/00 , F04B19/006 , G01N27/44704 , G01N27/44791
摘要： A microfabricated device employing a bridging membrane and methods for electrokinetic transport of a liquid phase biological or chemical material using the same are described. The bridging membrane is deployed in or adjacent to a microchannel and permits either ionic current flow or the transport of gas species, while inhibiting the bulk flow of material. The use of bridging membranes in accordance with this invention is applicable to a variety of processes, including electrokinetically induced pressure flow in a region of a microehannel that is not influenced by an electric field, sample concentration enhancement and injection, as well as improving the analysis of materials where it is desired to eliminate electrophoretic bias. Other applications of the bridging membranes according to this invention include the separation of species from a sample material, valving of fluids in a microchannel network, mixing of different materials in a microchannel, and the pumping of fluids.
摘要翻译：描述了使用桥接膜的微制造装置和使用其的液相生物或化学材料的电动转运方法。桥接膜部署在微通道中或邻近微通道，并允许离子电流流动或气体物质的输送，同时抑制材料的大量流动。根据本发明的桥接膜的使用适用于各种工艺，包括不受电场影响的微通道区域中的电动感应压力流，样品浓度增强和注入，以及改进分析需要消除电泳偏压的材料。根据本发明的桥接膜的其它应用包括物质与样品材料的分离，微通道网络中的流体阀门，微通道中的不同材料的混合以及流体的泵送。

10. 发明授权

US07727363B2 Microfluidic device and methods for focusing fluid streams using electroosmotically induced pressures 有权
标题翻译：微流体装置和使用电动感应压力聚焦流体流的方法
公开(公告)号：US07727363B2
公开(公告)日：2010-06-01
申请号：US11049543
申请日：2005-02-02
申请人： Stephen C. Jacobson , J. Michael Ramsey
发明人： Stephen C. Jacobson , J. Michael Ramsey
IPC分类号： F04B19/04 , F15C1/04 , F16K31/02
CPC分类号： C07K1/24 , Y10T137/0391 , Y10T137/2185 , Y10T137/2224
摘要： A microfabricated device employing a bridging membrane and methods for electrokinetic transport of a liquid phase biological or chemical material using the same are described. The bridging membrane is deployed in or adjacent to a microchannel and permits either electric current flow or the transport of gas species, while inhibiting the bulk flow of material. The use of bridging membranes in accordance with this invention is applicable to electrokinetically inducing fluid flow to confine a selected material in a region of a microchannel that is not influenced by an electric field. Other structures for inducing fluid flow in accordance with this invention include nanochannel bridging membranes and alternating current fluid pumping devices. Applications of the bridging membranes according to this invention include the separation of species from a sample material, valving of fluids in a microchannel network, mixing of different materials in a microchannel, and the pumping of fluids.
摘要翻译：描述了使用桥接膜的微制造装置和使用其的液相生物或化学材料的电动转运方法。桥接膜部署在微通道中或邻近微通道，并允许电流流动或气体物质的输送，同时抑制材料的大量流动。根据本发明的桥接膜的使用可应用于电动诱导流体流动以将所选择的材料限制在不受电场影响的微通道的区域中。根据本发明的用于诱导流体流动的其它结构包括纳米通道桥接膜和交流流体泵送装置。根据本发明的桥接膜的应用包括物质与样品材料的分离，微通道网络中的流体阀门，微通道中的不同材料的混合以及流体的泵送。

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