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    • 7. 发明授权
    • Alpha-amylase variants stabilized against dimerization and/or multimerization, method for the production thereof, and detergents and cleansers containing these alpha-amylase variants
    • 稳定抗二聚和/或多聚化的α-淀粉酶变体,其生产方法,以及含有这些α-淀粉酶变体的洗涤剂和清洁剂
    • US08785365B2
    • 2014-07-22
    • US11692691
    • 2007-03-28
    • Cornelius BesslerSusanne WielandKarl-Heinz Maurer
    • Cornelius BesslerSusanne WielandKarl-Heinz Maurer
    • C12N9/28C11D3/386C12S11/00C12S9/00
    • C12N9/2417C11D3/38681
    • The present invention relates to α-amylase variants that are stabilized to dimerization and/or multimerization, in particular at elevated temperatures or high pH, by point mutagenesis of positively polarized or charged or neutral surface amino acids to give more negatively polarized or charged amino acids. The invention further relates to methods of increasing the stability of an α-amylase to dimerization and/or multimerization brought about by electrostatic interactions whereby at least one amino acid residue on the surface of the starting molecule, which makes a neutral or positively polar or charged contribution to the electrostatic potential of said molecule, is replaced with a more negatively polar or negatively charged amino acid residue. The α-amylase variants obtained thereby exhibit better stability to influences of the solvent, increased processivity, and are suited for numerous industrial areas of use, in particular as active ingredients in detergents and cleansers.
    • 本发明涉及通过正极化或带电荷或中性表面氨基酸的点诱变稳定至二聚化和/或多聚化的α-淀粉酶变体,特别是在升高的温度或高pH下,以产生更多的负极化或带电荷的氨基酸 。 本发明还涉及增加α-淀粉酶对由静电相互作用引起的二聚化和/或多聚化的稳定性的方法,其中起始分子表面上至少有一个氨基酸残基,其形成中性或正极性或带电 对所述分子的静电电位的贡献被更负极性或带负电荷的氨基酸残基取代。 由此获得的α-淀粉酶变体对溶剂的影响表现出更好的稳定性,增加的持续性,并且适用于许多工业使用领域,特别是作为洗涤剂和清洁剂中的活性成分。