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    • 2. 发明授权
    • Enzyme-mediated synthesis of esters and lactones
    • 酶介导的酯和内酯的合成
    • US4451565A
    • 1984-05-29
    • US350519
    • 1982-02-19
    • Ian GatfieldTheodor Sand
    • Ian GatfieldTheodor Sand
    • C12P7/40C12P7/42C12P7/62C12P17/02C12P17/04C12P17/00C12P7/00C12R1/785
    • C12P17/04C12P7/62Y10S435/931
    • In the enzyme-mediated synthesis of an ester or lactone from at least one carboxylic acid of the formulaR--CH.sub.2 --CH.sub.2 --COOHwhereinR is a hydrogen atom, or a hydrocarbon radical which has 1 to 21 carbon atoms and which can be optionally substituted by hydroxyl groups or alkoxy groups with 1 to 10 carbon atoms,with at least one primary or secondary alcohol having 1 to 15 carbon atoms, the improvement which comprises employing as the enzyme the esterase from Mucor miehei and effecting the synthesis in the absence of water. Advantageously, the synthesis is effected at about room temperature, the mol ratio of carboxylic acid to alcohol is from about 1:1 to 1:1.1, and the enzyme is used in a quantity of about 3 to 20% by weight of the carboxylic acid. The absence of water facilitates recovery, as by distillation.
    • 在酶介导的由至少一种式R-CH 2 -CH 2 -COOH的羧酸(其中R是氢原子)或具有1至21个碳原子的烃基的酯或内酯的合成中,其可以是任选的 由具有1-10个碳原子的羟基或烷氧基取代,至少一个具有1至15个碳原子的伯醇或仲醇,其改进包括使用来自Mucor miehei的酯酶并在不存在下进行合成 水。 有利地,合成在约室温下进行,羧酸与醇的摩尔比为约1:1至1:1.1,酶的用量为羧酸的约3-20重量% 。 没有水有助于回收,如通过蒸馏。