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    • 8. 发明授权
    • Method for detecting protein-protein interactions in cells
    • 检测细胞中蛋白质 - 蛋白质相互作用的方法
    • US08877446B2
    • 2014-11-04
    • US13524868
    • 2012-06-15
    • Seung Goo LeeSu-Lim ChoiJong Sik GamJae Jun SongSang Jun Lee
    • Seung Goo LeeSu-Lim ChoiJong Sik GamJae Jun SongSang Jun Lee
    • C12Q1/68G01N33/542G01N33/50G01N21/64
    • G01N21/6428G01N33/5008G01N33/542
    • The present invention relates to a method for detecting protein-protein interactions in living cells, and more particularly, to a method for providing cells comprising a first construct and a second construct, wherein the first construct comprises a polynucleotide encoding a first fusion protein which comprises a bait protein, a first fluorescent protein and a CBD (cellulose-binding domain) protein, and wherein the second construct comprises a polynucleotide encoding a second fusion protein which comprises a prey protein and a second fluorescent protein so as to allow formation of inclusion bodies, and detecting interactions between the bait protein and the prey protein that are displayed by inclusion bodies, a method for isolating the prey protein bound to the bait protein using the cells comprising the constructs, the cells, and a kit for detecting protein-protein interactions, comprising the constructs.
    • 本发明涉及一种用于检测活细胞中蛋白质 - 蛋白质相互作用的方法,更具体地说,涉及提供包含第一构建体和第二构建体的细胞的方法,其中第一构建体包含编码第一融合蛋白的多核苷酸,其包含 诱饵蛋白质,第一荧光蛋白质和CBD(纤维素结合结构域)蛋白质,并且其中第二构建体包含编码第二融合蛋白的多核苷酸,其包含猎物蛋白质和第二荧光蛋白质,以便形成包涵体 并检测由包涵体显示的诱饵蛋白和猎物蛋白之间的相互作用,使用包含构建体的细胞分离与诱饵蛋白结合的猎物蛋白的方法,所述细胞和用于检测蛋白质 - 蛋白质相互作用的试剂盒 ,包括构建体。