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    • 1. 发明申请
    • Method and kit for performing functional tests on biological cells
    • 用于对生物细胞进行功能测试的方法和试剂盒
    • US20050214832A1
    • 2005-09-29
    • US11051315
    • 2005-02-04
    • Hugo HaemmerleThomas JoosMarkus TemplinHansjurgen VolkmerKerstin RagnitzSusanne StumpfCornelia Kuschel
    • Hugo HaemmerleThomas JoosMarkus TemplinHansjurgen VolkmerKerstin RagnitzSusanne StumpfCornelia Kuschel
    • C12Q1/68G01N33/50G01N33/53G01N33/567
    • G01N33/5005
    • A process for carrying out functional assays on test cells is described comprising the following steps: a) providing an array of measuring points which are separate from each other and to which in each case capture molecules to which said test cells can bind are immobilized, b) generating a mixture of said test cells and of reference particles which are capable of binding to said capture molecules and which are distinguishable from said test cells, c) contacting the mixture of step b) with the array so that test cells and reference particles can bind to each measuring point, and d) determining the ratio of bound test cells of interest to bound reference particles for at least some of the measuring points. In a further process, at least one measuring point with its assigned capture molecules is distributed between a plurality of measuring areas in the array, which are arranged at various sites in said array. In another process, the array is agitated, after contacting with the test cells and, where appropriate, the reference particles.
    • 描述了用于在测试细胞上进行功能测定的方法,其包括以下步骤:a)提供彼此分离的测量点阵列,并且在每种情况下捕获所述测试细胞可以结合的分子被固定化,b )产生所述测试细胞和能够结合所述捕获分子并且可与所述测试细胞区分的参考颗粒的混合物,c)使步骤b)的混合物与阵列接触,使得测试细胞和参考颗粒可以 结合到每个测量点,以及d)确定至少一些测量点的目标结合测试细胞与结合参考颗粒的比例。 在另一个过程中,具有其分配的捕获分子的至少一个测量点分布在阵列中的多个测量区域之间,其被布置在所述阵列中的各个位置。 在另一个方法中,在与测试电池接触并且在适当的时候搅拌参考颗粒。