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1. 发明申请

US20120006090A1 LIGHT GUIDE PLATE STAMP AND METHOD OF MANUFACTURING THE SAME 有权
标题翻译：光导板印章及其制造方法
公开(公告)号：US20120006090A1
公开(公告)日：2012-01-12
申请号：US13240982
申请日：2011-09-22
申请人： Ho-Han RYU , Tae-Seok Kim , Seung-Pyo Hong , Seung-Yeop Lee
发明人： Ho-Han RYU , Tae-Seok Kim , Seung-Pyo Hong , Seung-Yeop Lee
IPC分类号： B21D37/01
CPC分类号： B21D47/04
摘要： A stamp includes a metal supporting layer, a pattern forming layer and an adhesive layer. The metal supporting layer has a first thermal conductivity. The pattern forming layer is disposed on the metal supporting layer and has a surface with a molding pattern formed thereon. The adhesive layer is disposed between the metal supporting layer and the pattern forming layer to couple the pattern forming layer to the metal supporting layer, and has a second thermal conductivity lower than the first thermal conductivity. Thus, strength of the stamp may be improved, and deformation of the stamp during the process of manufacturing a light guide plate may be reduced or prevented.
摘要翻译：印模包括金属支撑层，图案形成层和粘合剂层。金属支撑层具有第一导热性。图案形成层设置在金属支撑层上，并具有在其上形成有模制图案的表面。粘合剂层设置在金属支撑层和图案形成层之间，以将图案形成层耦合到金属支撑层，并且具有比第一热导率低的第二热导率。因此，可以提高印模的强度，并且可以减少或防止在制造导光板的过程中印模的变形。

2. 发明授权

US08616875B2 Light guide plate stamp and method of manufacturing the same 有权
标题翻译：导光板印章及其制造方法
公开(公告)号：US08616875B2
公开(公告)日：2013-12-31
申请号：US13240982
申请日：2011-09-22
申请人： Ho-Han Ryu , Tae-Seok Kim , Seung-Pyo Hong , Seung-Yeop Lee
发明人： Ho-Han Ryu , Tae-Seok Kim , Seung-Pyo Hong , Seung-Yeop Lee
IPC分类号： B29C45/34 , F21V7/04 , B29D7/00
CPC分类号： B21D47/04
摘要： A stamp includes a metal supporting layer, a pattern forming layer and an adhesive layer. The metal supporting layer has a first thermal conductivity. The pattern forming layer is disposed on the metal supporting layer and has a surface with a molding pattern formed thereon. The adhesive layer is disposed between the metal supporting layer and the pattern forming layer to couple the pattern forming layer to the metal supporting layer, and has a second thermal conductivity lower than the first thermal conductivity. Thus, strength of the stamp may be improved, and deformation of the stamp during the process of manufacturing a light guide plate may be reduced or prevented.
摘要翻译：印模包括金属支撑层，图案形成层和粘合剂层。金属支撑层具有第一导热性。图案形成层设置在金属支撑层上，并具有在其上形成有模制图案的表面。粘合剂层设置在金属支撑层和图案形成层之间，以将图案形成层耦合到金属支撑层，并且具有比第一热导率低的第二热导率。因此，可以提高印模的强度，并且可以减少或防止在制造导光板的过程中印模的变形。

3. 发明授权

US08785164B2 Expression of caleosin in recombinant oleaginous microorganisms to increase oil content therein 失效
标题翻译：在重组含油微生物中表达caleosin以增加其中的油含量
公开(公告)号：US08785164B2
公开(公告)日：2014-07-22
申请号：US13478522
申请日：2012-05-23
申请人： Seung-Pyo Hong , Quinn Qun Zhu
发明人： Seung-Pyo Hong , Quinn Qun Zhu
IPC分类号： C12P7/64 , C12N1/00
CPC分类号： C07K14/37 , C12P7/6463 , C12P7/6472
摘要： Recombinant oleaginous microorganisms having increased oil content due to the expression of a caleosin polypeptide are described. A recombinant oleaginous microorganism of the disclosed invention produces at least 25% of its dry cell weight as oil, and comprises a functional polyunsaturated fatty acid (PUFA) biosynthetic pathway and at least one genetic construct encoding a caleosin polypeptide. A method for increasing the amount of oil in a recombinant oleaginous microorganism is also described.
摘要翻译：描述了由于Caleosin多肽的表达而具有增加的油含量的重组含油微生物。本发明公开的本发明的重组含油微生物作为油产生至少25％的其干细胞重量，并且包含功能性多不饱和脂肪酸（PUFA）生物合成途径和至少一种编码卡罗多肽多肽的遗传构建体。还描述了用于增加重组含油微生物中的油量的方法。

4. 发明授权

US08703473B2 Recombinant microbial host cells for high eicosapentaenoic acid production 有权
标题翻译：用于高二十碳五烯酸生产的重组微生物宿主细胞
公开(公告)号：US08703473B2
公开(公告)日：2014-04-22
申请号：US13218708
申请日：2011-08-26
申请人： Seung-Pyo Hong , Pamela L. Sharpe , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Seung-Pyo Hong , Pamela L. Sharpe , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
IPC分类号： C12N1/00 , C12N1/20 , C12P7/64
CPC分类号： C12N9/1029 , C12N9/0071 , C12N15/815 , C12P7/6472
摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica are disclosed herein that are capable of producing microbial oil comprising greater than 25 weight percent of eicosapentaenoic acid [“EPA”], an omega-3 polyunsaturated fatty acid, measured as a weight percent of dry cell weight.
摘要翻译：本文公开了含油酵母解脂耶氏酵母解脂耶氏酵母的工程菌株，其能够产生包含大于25重量％的二十碳五烯酸[“EPA”]的微生物油，其为以干细胞重量的重量百分比测量的ω-3多不饱和脂肪酸。

5. 发明授权

US08435758B2 Manipulation of SNF1 kinase for altered oil content in oleaginous organisms 有权
标题翻译：操纵SNF1激酶以改善含油生物体内油含量
公开(公告)号：US08435758B2
公开(公告)日：2013-05-07
申请号：US12549439
申请日：2009-08-28
申请人： Seung-Pyo Hong , Quinn Qun Zhu , John E. Seip
发明人： Seung-Pyo Hong , Quinn Qun Zhu , John E. Seip
IPC分类号： C12P1/00 , C07H21/04 , C12N1/15
CPC分类号： C12P7/6472 , C12P7/6427 , C12P7/6463
摘要： Methods of increasing the total lipid content in a eukaryotic cell, the total content of polyunsaturated fatty acids [“PUFAs”], and/or the ratio of desaturated fatty acids to saturated fatty acids by reducing the activity of the heterotrimeric SNF1 protein kinase are disclosed. Preferably, the chromosomal genes encoding the Snf1 α-subunit, Gal83 β-subunit or Snf4 γ-subunit of the SNF1 protein kinase, the upstream regulatory genes encoding Sak1, Hxk2, Glk1 or Reg1, or the downstream genes encoding Rme1, Cbr1 or Snf3 are manipulated in a PUFA-producing strain of the oleaginous yeast Yarrowia lipolytica, resulting in increased total lipid content, as compared to the parent strain comprising the heterotrimeric SNF1 protein kinase not having reduced activity.
摘要翻译：公开了通过降低异源三聚SNF1蛋白激酶的活性来增加真核细胞中总脂质含量，多不饱和脂肪酸的总含量[“PUFA”]和/或去饱和脂肪酸与饱和脂肪酸的比例的方法。优选地，编码SNF1蛋白激酶的Snf1α-亚基，Gal83β-亚基或Snf4γ-亚基的染色体基因，编码Sak1，Hxk2，Glk1或Reg1的上游调控基因或编码Rme1，Cbr1或Snf3的下游基因与含有不具有降低的活性的异源三聚SNF1蛋白激酶的亲本菌株相比，在含油酵母解脂耶氏酵母解脂耶氏酵母解脂耶氏酵母的PUFA生产菌株中操作，导致总脂质含量增加。

6. 发明授权

US08399226B2 High eicosapentaenoic acid oils from improved optimized strains of Yarrowia lipolytica 有权
标题翻译：来自改良的解脂耶氏酵母菌株的高二十碳五烯酸油
公开(公告)号：US08399226B2
公开(公告)日：2013-03-19
申请号：US12814880
申请日：2010-06-14
申请人： Seung-Pyo Hong , Pamela L. Sharpe , Zhixiong Xue , Narendra S. Yadav , Quinn Qun Zhu
发明人： Seung-Pyo Hong , Pamela L. Sharpe , Zhixiong Xue , Narendra S. Yadav , Quinn Qun Zhu
IPC分类号： C12P7/64 , A23D9/00
CPC分类号： C12N9/1029 , A21D2/165 , A23K20/158 , C12N9/00 , C12N9/13 , C12N9/93 , C12P7/6427 , C12P7/6472 , C12P7/6481
摘要： Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids [“% TFAs”] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The strains possess at least one peroxisome biogenesis factor protein knockout. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.
摘要翻译：描述了能够产生包含大于50重量％的二十碳五烯酸[EPA]，ω-3多不饱和脂肪酸的脂肪酵母解脂耶氏酵母的工程菌株，以总脂肪酸的重量％[％TFA]和具有至少3.1％的EPA％TFA与亚油酸的比例，以％TFA测定。除了其他异源＆Dgr .9延伸酶，Dgr。8去饱和酶，Dgr。5去饱和酶，Dgr 17脱饱和酶，Dgr。12去饱和酶，C16外，这些菌株过度表达至少一种Dgr 9延伸酶/ / 18延伸酶和任选的过表达二酰基甘油胆碱磷酸转移酶，丙二酰辅酶A合成酶和/或酰基辅酶A溶血磷脂酰基转移酶。该菌株具有至少一种过氧化物酶体生物发生因子蛋白质敲除。要求在所述宿主细胞内生产EPA的方法，从细胞获得的油及其产物。

7. 发明申请

US20090186804A1 ANTICOAGULANT AND COMPOSITION FOR PREVENTING THROMBUS CONTAINING POLY-GAMMA-GLUTAMIC ACID 审中-公开
标题翻译：用于预防含有多糖 - 谷氨酸的含沙门氏菌的抗凝血剂和组合物
公开(公告)号：US20090186804A1
公开(公告)日：2009-07-23
申请号：US12159561
申请日：2006-12-22
申请人： Moon-Hee Sung , Chung Park , Seung-Pyo Hong , Haryoung Poo , Tae-Woo Kim
发明人： Moon-Hee Sung , Chung Park , Seung-Pyo Hong , Haryoung Poo , Tae-Woo Kim
IPC分类号： A61K38/00 , A61P7/02
CPC分类号： A61K31/74 , A23L33/175 , A61K9/0095
摘要： The present invention relates to an anticoagulant and a composition for preventing thrombus formation, which contain poly-gamma-glutamic acid (PGA) as an active ingredient. The inventive PGA is a water-soluble, anionic, biodegradable and edible amino acid polymer material, which has an anticoagulant effect of preventing thrombi from being accumulated in blood vessels, shows an excellent sustained-release effect and is harmless to the human body. Thus, it is useful as a high-value-added anticoagulant and a food or beverage composition for preventing thrombus formation.
摘要翻译：本发明涉及含有聚-γ-谷氨酸（PGA）作为活性成分的用于预防血栓形成的抗凝血剂和组合物。本发明的PGA是一种水溶性，阴离子，可生物降解和可食用的氨基酸聚合物材料，其具有防止血栓在血管中积聚的抗凝作用，显示出优异的持续释放作用并对人体无害。因此，作为高附加值抗凝剂和用于预防血栓形成的食品或饮料组合物是有用的。

8. 发明授权

US07501262B2 Promoters and gene expression method by using the promoters 有权
标题翻译：启动子和使用启动子的基因表达方法
公开(公告)号：US07501262B2
公开(公告)日：2009-03-10
申请号：US11599475
申请日：2006-11-15
申请人： Moon-Hee Sung , Seung-Goo Lee , Seung-Pyo Hong , Hwa-Jung Seo
发明人： Moon-Hee Sung , Seung-Goo Lee , Seung-Pyo Hong , Hwa-Jung Seo
IPC分类号： C12P21/00 , C12N1/21 , C12N15/70 , C12N15/74 , C07H21/04
CPC分类号： C07K14/32 , C12N15/70 , C12N15/75
摘要： To provide a promoter, a recombinant DNA, a gene expression vector, an expression vector, and a transformant, which are capable of expressing a gene without inducing gene expression with an inducer; and a method for producing a protein and a kit therefor, which can be operated easily and performed inexpensively by convenient and inexpensive steps. An isolated DNA having the nucleotide sequence of SEQ ID NO: 1 or 2 of Sequence Listing or a fragment thereof, wherein the isolated DNA exhibits a constitutive promoter activity in Escherichia coli or a bacterium belonging to the genus Bacillus; an isolated DNA having a nucleotide sequence of a nucleic acid capable of hybridizing to the above DNA, wherein the isolated DNA exhibits a constitutive promoter activity in Escherichia coli or a bacterium belonging to the genus Bacillus; a recombinant DNA comprising the above DNA and a foreign gene, wherein the foreign gene is operably located; a gene expression vector, at least comprising the above DNA; an expression vector comprising the recombinant DNA; a transformant having the above recombinant DNA, or the above expression vector; a method for producing a protein, characterized by culturing the above transformant, and collecting a protein from the resulting culture; and a kit for producing a protein, at least comprising the above DNA, or the above gene expression vector.
摘要翻译：提供能够表达基因而不诱导基因表达的启动子，重组DNA，基因表达载体，表达载体和转化体; 以及用于制备蛋白质及其试剂盒的方法，其可以容易地操作并且通过方便和便宜的步骤廉价地进行。具有序列表的SEQ ID NO：1或2的核苷酸序列或其片段的分离的DNA，其中所述分离的DNA在大肠杆菌或属于芽孢杆菌属的细菌中显示组成型启动子活性; 具有能够与上述DNA杂交的核酸的核苷酸序列的分离的DNA，其中所述分离的DNA在大肠杆菌或属于芽孢杆菌属的细菌中显示出组成型启动子活性; 包含上述DNA和外来基因的重组DNA，其中所述外源基因可操作地定位; 至少包含上述DNA的基因表达载体; 包含重组DNA的表达载体; 具有上述重组DNA的转化体或上述表达载体; 一种生产蛋白质的方法，其特征在于培养上述转化体，并从得到的培养物中收集蛋白质; 以及用于生产至少包含上述DNA或上述基因表达载体的蛋白质的试剂盒。

9. 外观设计

USD529005S1 Television set 有权
公开(公告)号：USD529005S1
公开(公告)日：2006-09-26
申请号：US29230228
申请日：2005-05-19
申请人： Seung-Pyo Hong
设计人： Seung-Pyo Hong

10. 发明申请

US20120052537A1 RECOMBINANT MICROBIAL HOST CELLS FOR HIGH EICOSAPENTAENOIC ACID PRODUCTION 有权
标题翻译：用于高效酸生产的重组微生物宿主细胞
公开(公告)号：US20120052537A1
公开(公告)日：2012-03-01
申请号：US13218708
申请日：2011-08-26
申请人： Seung-Pyo Hong , Pamela L. Sharpe , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Seung-Pyo Hong , Pamela L. Sharpe , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
IPC分类号： C12P7/64 , C12N1/19 , C12N1/00
CPC分类号： C12N9/1029 , C12N9/0071 , C12N15/815 , C12P7/6472
摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica are disclosed herein that are capable of producing microbial oil comprising greater than 25 weight percent of eicosapentaenoic acid [“EPA”], an omega-3 polyunsaturated fatty acid, measured as a weight percent of dry cell weight.
摘要翻译：本文公开了含油酵母解脂耶氏酵母解脂耶氏酵母的工程菌株，其能够产生包含大于25重量％的二十碳五烯酸[“EPA”]的微生物油，其为以干细胞重量的重量百分比测量的ω-3多不饱和脂肪酸。

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