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1. 发明申请

US20110229980A1 OLIGONUCLEOTIDE PROBE AND USE THEREOF 审中-公开
标题翻译：寡核苷酸探针及其用途
公开(公告)号：US20110229980A1
公开(公告)日：2011-09-22
申请号：US12982931
申请日：2010-12-31
申请人： Hiroyuki ASANUMA , Xingguo Liang , Hiromu Kashida , Yasuko Yoshida , Tomokazu Takase , Kousuke Niwa
发明人： Hiroyuki ASANUMA , Xingguo Liang , Hiromu Kashida , Yasuko Yoshida , Tomokazu Takase , Kousuke Niwa
IPC分类号： G01N33/53 , C07H21/00
CPC分类号： C12Q1/6816 , C12Q2525/301 , C12Q2565/501
摘要： The present teaching provides a fluorescent oligonucleotide probe having a high degree of design flexibility and wide applicability, as well as the use thereof. This is an oligonucleotide probe capable of forming a stem and loop, comprising at least one fluorophore located between adjacent nucleotides in the stem and is linked to a unit represented by Formula (1) and at least one quencher located at a site capable of pairing up with the at least one fluorophore located between the adjacent nucleotides in the stem and is linked to a unit represented by Formula (2). (In the formulae, X represents the fluorophore, Y represents the quencher, R1 represents an optionally substituted C2 or C3 alkylene chain, R2 represents an optionally substituted C0-2 alkylene chain, and Z represents a direct bond or linker.)
摘要翻译：本教导提供具有高设计灵活性和广泛适用性的荧光寡核苷酸探针及其用途。这是能够形成茎和环的寡核苷酸探针，其包含位于茎中相邻核苷酸之间的至少一个荧光团，并连接到由式（1）表示的单元和位于能够配对的位点处的至少一个猝灭剂其中所述至少一个荧光团位于茎中相邻核苷酸之间，并与由式（2）表示的单元连接。（式中，X表示荧光团，Y表示猝灭剂，R 1表示任选取代的C 2或C 3亚烷基链，R 2表示任选取代的C 0-2亚烷基链，Z表示直接键或连接基。

2. 发明申请

US20050059037A1 Nucleotide derivative and DNA microarray 有权
标题翻译：核苷酸衍生物和DNA微阵列
公开(公告)号：US20050059037A1
公开(公告)日：2005-03-17
申请号：US10795436
申请日：2004-03-09
申请人： Isao Saito , Akimitsu Okamoto , Yoshio Saito , Yasuko Yoshida , Kousuke Niwa
发明人： Isao Saito , Akimitsu Okamoto , Yoshio Saito , Yasuko Yoshida , Kousuke Niwa
IPC分类号： C07H19/04 , C07H19/067 , C07H21/00 , C07H21/04 , C12N15/09 , C12Q1/68 , G01N33/58
CPC分类号： C07H19/04 , C07H19/06 , C07H19/067 , C07H19/16 , C07H21/00 , C07H21/04 , C12Q1/6837 , Y10S436/809 , C12Q2565/549
摘要： A novel nucleotide derivative, in case of existing as a member of a single-stranded sequence, undergoing a change in the fluorescent signal intensity depending on the corresponding base type in the partner strand with which the single-stranded sequence is hybridized, and which is a thymine/uracil derivative (1) emitting light most intensely when a confronting base in the partner strand with which the single-stranded nucleotide sequence is hybridized is adenine; a cytosine derivative (2) emitting light most intensely when the confronting base is guanine; an adenine derivative (3) emitting light most intensely when the confronting base is cytosine; a guanine derivative (4) emitting light most intensely when the confronting base is cytosine or thymine/uracil; and an adenine derivative (5) emitting light most intensely when the confronting base is thymine/uracil.
摘要翻译：在存在单链序列成员的情况下，新的核苷酸衍生物根据与单链序列杂交的伴侣链中的相应碱基类型经历荧光信号强度的变化，并且其是当与单链核苷酸序列杂交的伴侣链中的相对的碱基是腺嘌呤时，最强烈地发射光的胸腺嘧啶/尿嘧啶衍生物（1）当相对的碱是鸟嘌呤时，胞嘧啶衍生物（2）最强地发射光; 当面对的碱基是胞嘧啶时，腺苷衍生物（3）最强地发光; 当面对的碱基是胞嘧啶或胸腺嘧啶/尿嘧啶时，最强烈地发射光的鸟嘌呤衍生物（4）和当面对的碱是胸腺嘧啶/尿嘧啶时最强烈地发光的腺嘌呤衍生物（5）。

3. 发明授权

US07414117B2 Nucleotide derivative and DNA microarray 有权
标题翻译：核苷酸衍生物和DNA微阵列
公开(公告)号：US07414117B2
公开(公告)日：2008-08-19
申请号：US10795436
申请日：2004-03-09
申请人： Isao Saito , Akimitsu Okamoto , Yoshio Saito , Yasuko Yoshida , Kousuke Niwa
发明人： Isao Saito , Akimitsu Okamoto , Yoshio Saito , Yasuko Yoshida , Kousuke Niwa
IPC分类号： C07H21/02 , C07H19/04 , C12Q1/68 , G01N33/53 , C07H21/00
CPC分类号： C07H19/04 , C07H19/06 , C07H19/067 , C07H19/16 , C07H21/00 , C07H21/04 , C12Q1/6837 , Y10S436/809 , C12Q2565/549
摘要： A novel nucleotide derivative, in case of existing as a member of a single-stranded sequence, undergoing a change in the fluorescent signal intendity depending on the corresponding base type in the partner strand with which the single-stranded sequence is hybridized, and which is a thymin/uracil derivative (1) emitting light most intensely when a confronting base in the partner strand with which the single-stranded nucleotide sequence is hybridized is adenine; a cytosine derivative (2) emitting light most intensely when the confronting base is guanine; an adenine derivative (3) emitting light most intensely when the confronting base is cytosine; a guanine derivative (4) emitting light most intensely when the confronting base is cytosine or thymine/uracil; and an adrnine derivative (5) emitting light most intensely when the confronting base is thymine/uracil/.
摘要翻译：在存在单链序列成员的情况下，新的核苷酸衍生物依赖于与单链序列杂交的伴侣链中的相应碱基类型而经历荧光信号意图的变化，并且其是当与单链核苷酸序列杂交的伴侣链中的相对的碱基是腺嘌呤时，最强烈地发射光的甲氨喋呤/尿嘧啶衍生物（1）当相对的碱是鸟嘌呤时，胞嘧啶衍生物（2）最强地发射光; 当面对的碱基是胞嘧啶时，腺苷衍生物（3）最强地发光; 当面对的碱基是胞嘧啶或胸腺嘧啶/尿嘧啶时，最强烈地发射光的鸟嘌呤衍生物（4）和当相对底物是胸腺嘧啶/尿嘧啶时最强烈地发光的腺苷衍生物（5）。

4. 发明申请

US20080200347A1 Array and Hybridization Method 审中-公开
标题翻译：阵列和杂交方法
公开(公告)号：US20080200347A1
公开(公告)日：2008-08-21
申请号：US11794811
申请日：2006-01-16
申请人： Yasuko Yoshida , Kazunari Yamada , Kousuke Niwa
发明人： Yasuko Yoshida , Kazunari Yamada , Kousuke Niwa
IPC分类号： C40B40/06 , C40B50/08 , C40B50/14 , C40B60/14
CPC分类号： C12Q1/6837 , B01J2219/00286 , B01J2219/005 , B01J2219/00527 , B01J2219/00542 , B01J2219/00545 , B01J2219/00554 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00722 , B01L3/508 , B01L2300/0636 , B01L2300/0822
摘要： The present invention provides a hybridization method suited for using a signal probe. An array of the present invention comprises: a substrate; a nucleic acid probe that is fixed to the substrate and is hybridized with a sample to have a signal change; and at least one cavity that is filled with a specific liquid containing the sample and causing the hybridization of the nucleic acid probe with the sample. The array in this arrangement effectively enhances the reproducibility and the efficiency of hybridization with the signal probe.
摘要翻译：本发明提供了适合于使用信号探针的杂交方法。本发明的阵列包括：基底; 固定在基底上并与样品杂交以具有信号变化的核酸探针; 以及填充有含有样品的特定液体并使核酸探针与样品杂交的至少一个空腔。在这种布置中的阵列有效地增强了与信号探针杂交的重现性和效率。

5. 发明申请

US20130115718A1 Carrier Carrying Identifying Information for Identifying an Identification Subject and Use Thereof 审中-公开
标题翻译：承运人携带身份识别信息识别标识和使用
公开(公告)号：US20130115718A1
公开(公告)日：2013-05-09
申请号：US13810477
申请日：2011-07-15
申请人： Toshikazu Hirota , Kousuke Niwa
发明人： Toshikazu Hirota , Kousuke Niwa
IPC分类号： C12Q1/68 , C07H21/04 , G01N33/566
CPC分类号： B42D25/29 , B42D25/00 , B42D25/21 , B42D25/23 , B42D2033/20 , C12Q2563/179 , C12Q2563/185 , G07D7/14
摘要： Disclosed are a carrier that carries identifying information having favorable identification performance and a use thereof. The carrier carries identifying information for identifying an identification subject, and comprises one or more pieces of DNA having a thymine-rich base sequence and having, as the identifying information, an identifying base sequence pre-associated with the identification subject.
摘要翻译：公开了携带具有良好识别性能的识别信息及其使用的载体。载体携带用于识别识别对象的识别信息，并且包括具有富含胸腺嘧啶碱基序列的一个或多个DNA，并且具有作为识别信息的与识别对象预先相关联的识别碱基序列。

6. 发明申请

US20120178648A1 METHOD FOR DETECTION OF TARGET NUCLEIC ACID 有权
标题翻译：检测目标核酸的方法
公开(公告)号：US20120178648A1
公开(公告)日：2012-07-12
申请号：US13497000
申请日：2010-10-26
申请人： Mitsuo Kawase , Toshikazu Hirota , Kousuke Niwa
发明人： Mitsuo Kawase , Toshikazu Hirota , Kousuke Niwa
IPC分类号： C40B30/04 , C40B40/06
CPC分类号： C12Q1/689 , C12Q1/6858 , C12Q2600/158 , C12Q2531/107 , C12Q2537/143 , C12Q2563/107 , C12Q2565/514
摘要： An object of the disclosure of the present specification is to provide a method for detection of a target nucleic acid which allows construction of an effective detection system of a target nucleic acid. For this purpose, in the disclosure of the present specification, a first primer comprising an identification sequence complementary to a target sequence in a target nucleic acid and a tag addition sequence, and a second primer having a label are prepared. The first primer and the second primer are used for the target nucleic acid in a sample to amplify a chimeric DNA having a tag sequence and the label. The chimeric DNA is hybridized with a detection probe on a solid phase to obtain signal intensity information based on the label, and the target nucleic acid is detected based on the signal intensity information.
摘要翻译：本说明书的公开内容的一个目的是提供一种检测目标核酸的方法，其允许构建靶核酸的有效检测系统。为此，在本说明书的公开内容中，制备包含与靶核酸中的靶序列互补的鉴定序列和标签添加序列的第一引物和具有标记的第二引物。将第一引物和第二引物用于样品中的靶核酸以扩增具有标签序列和标记的嵌合DNA。嵌合DNA与固相上的检测探针杂交，得到基于标记的信号强度信息，根据信号强度信息检测靶核酸。

7. 发明申请

US20100276309A1 FLUID CONTAINING CARTRIDGE AND UTILIZATION OF THE SAME 审中-公开
标题翻译：含有流体的容器及其使用
公开(公告)号：US20100276309A1
公开(公告)日：2010-11-04
申请号：US12747597
申请日：2008-12-12
申请人： Masahiro Murasato , Kazunari Yamada , Dai Yamamoto , Akinobu Oribe , Kousuke Niwa
发明人： Masahiro Murasato , Kazunari Yamada , Dai Yamamoto , Akinobu Oribe , Kousuke Niwa
IPC分类号： B65D25/08 , B65D21/02
CPC分类号： B01F13/0222 , B01F13/0059 , B01L3/502715 , B01L3/502753 , B01L7/52 , B01L2200/04 , B01L2200/16 , B01L2300/0636 , B01L2300/0681 , B01L2300/0803 , B01L2300/0864 , B01L2300/0867 , B01L2400/0409 , B01L2400/0487 , Y10T137/8593
摘要： In a state where liquids are contained in a plurality of liquid container portions which are formed in a first layer and a second layer of a cartridge and which have predetermined volumes determined depending on liquids to be contained, and where the reaction vessel is connected to any one of communicating ports, when pressure is applied to act upon one of the liquid container portions which is communicated with the communicating port connected to the reaction vessel, the atmosphere is supplied to the one liquid container portion through a atmosphere flowing passage communicating with the outside, and the liquid contained in the one liquid container portion is supplied to the reaction vessel. The cartridge is rotated to connect another communicating port to the reaction vessel such that plural liquids are eventually supplied to the reaction vessel. On that occasion, since the atmosphere supplied through the atmosphere flowing passage is further supplied to the reaction vessel through the communicating port connected to the reaction vessel and the one liquid container portion, the plural liquids contained in the reaction vessel are stirred by the inflow of the atmosphere.
摘要翻译：在液体被容纳在形成在第一层中的多个液体容器部分和盒的第二层的状态下，并且具有根据要包含的液体确定的预定体积，并且其中反应容器连接到任何一个连通端口，当施加压力作用在与连接到反应容器的连通口连通的液体容器部分之一时，气氛通过与外部连通的大气流动通道供应到一个液体容器部分并且容纳在一个液体容器部分中的液体被供应到反应容器。旋转墨盒以将另一个连通口连接到反应容器，使得多个液体最终被供应到反应容器。因此，由于通过大气流动通道供应的气氛通过连接到反应容器和一个液体容器部分的连通口进一步供应到反应容器中，所以反应容器中包含的多种液体被流入气氛。

8. 发明授权

US09175339B2 Method for detection of target nucleic acid 有权
标题翻译：检测靶核酸的方法
公开(公告)号：US09175339B2
公开(公告)日：2015-11-03
申请号：US13497000
申请日：2010-10-26
申请人： Mitsuo Kawase , Toshikazu Hirota , Kousuke Niwa
发明人： Mitsuo Kawase , Toshikazu Hirota , Kousuke Niwa
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q1/6858 , C12Q2600/158 , C12Q2531/107 , C12Q2537/143 , C12Q2563/107 , C12Q2565/514
摘要： An object of the disclosure of the present specification is to provide a method for detection of a target nucleic acid which allows construction of an effective detection system of a target nucleic acid. For this purpose, in the disclosure of the present specification, a first primer comprising an identification sequence complementary to a target sequence in a target nucleic acid and a tag addition sequence, and a second primer having a label are prepared. The first primer and the second primer are used for the target nucleic acid in a sample to amplify a chimeric DNA having a tag sequence and the label. The chimeric DNA is hybridized with a detection probe on a solid phase to obtain signal intensity information based on the label, and the target nucleic acid is detected based on the signal intensity information.
摘要翻译：本说明书的公开内容的一个目的是提供一种检测目标核酸的方法，其允许构建目标核酸的有效检测系统。为此，在本说明书的公开内容中，制备包含与靶核酸中的靶序列互补的鉴定序列和标签添加序列的第一引物和具有标记的第二引物。将第一引物和第二引物用于样品中的靶核酸以扩增具有标签序列和标记的嵌合DNA。嵌合DNA与固相上的检测探针杂交，得到基于标记的信号强度信息，根据信号强度信息检测靶核酸。

9. 发明申请

US20120196765A1 METHOD FOR DETECTION OR ANALYSIS OF TARGET SEQUENCE IN GENOMIC DNA 审中-公开
标题翻译：用于检测或分析基因组DNA中目标序列的方法
公开(公告)号：US20120196765A1
公开(公告)日：2012-08-02
申请号：US13382594
申请日：2010-07-09
申请人： Mitsuo Kawase , Toshikazu Hirota , Kousuke Niwa
发明人： Mitsuo Kawase , Toshikazu Hirota , Kousuke Niwa
IPC分类号： C40B30/04 , C40B40/06
CPC分类号： C12Q1/6827 , C12Q1/6837 , C12Q2565/519 , C12Q2561/125
摘要： A method of detecting or analyzing a target sequence in a genomic DNA by using a capture probe immobilized on a solid carrier includes: bringing the target nucleic acid into contact with a first query probe that has a sequence complementary to a portion of the target sequence or to a sequence adjacent to the portion and a second query probe that has a sequence complementary to another portion of the target sequence or to a sequence adjacent to the another portion and that has a recognition sequence complementary to a portion of the capture probe; acquiring a ligated molecule by ligating the first query probe and the second query probe that are hybridized to the target nucleic acid; bringing the ligated molecule into contact with the capture probe on the solid carrier and then capturing the ligated molecule on the solid carrier by hybridizing the capture probe with the recognition sequence in the ligated molecule; and detecting the captured ligated molecule.
摘要翻译：通过使用固定在固体载体上的捕获探针来检测或分析基因组DNA中的靶序列的方法包括：使靶核酸与具有与靶序列的一部分互补的序列的第一查询探针接触，或与所述部分相邻的序列和第二查询探针，其具有与所述靶序列的另一部分互补的序列或与所述另一部分相邻的序列并具有与所述捕获探针的一部分互补的识别序列; 通过连接与靶核酸杂交的第一查询探针和第二查询探针来获得连接的分子; 使连接的分子与固体载体上的捕获探针接触，然后通过将捕获探针与连接的分子中的识别序列杂交捕获固定载体上的连接分子; 并检测捕获的结扎分子。

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