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1. 发明授权

US06869934B2 Method of purifying calcium ion-binding protein 失效
标题翻译：钙离子结合蛋白的纯化方法
公开(公告)号：US06869934B2
公开(公告)日：2005-03-22
申请号：US10088588
申请日：2001-07-18
申请人： Hiroshi Mizokami , Shinichi Furukawa , Keishin Sugawara , Tatsufumi Onchi , Kazuhiro Komatsu , Satoshi Koyanagi , Hideo Yoshizaki
发明人： Hiroshi Mizokami , Shinichi Furukawa , Keishin Sugawara , Tatsufumi Onchi , Kazuhiro Komatsu , Satoshi Koyanagi , Hideo Yoshizaki
IPC分类号： C07K14/47 , C12N9/64 , A61K38/00
CPC分类号： C12N9/6432 , C07K14/4721 , C07K14/4728 , C12Y304/21006
摘要： The present invention relates to a method for purifying a calcium ion-binding protein by cation exchange chromatography. The present invention provide a method for isolating and purifying a calcium ion-binding protein in a simple and efficient manner from a liquid sample containing a calcium ion-binding protein and contaminants without any pretreatment such as addition of a chelating agent. More specifically, the present invention relates to a method for purifying a calcium ion-binding protein which comprises contacting said protein with a cation exchange carrier in the presence of calcium ions to let the said protein be adsorbed to the carrier, and after washing, eluting said protein, and to a calcium ion-binding protein having substantially no contaminants obtained by the method of the present invention.
摘要翻译：本发明涉及通过阳离子交换层析纯化钙离子结合蛋白的方法。本发明提供了从含有钙离子结合蛋白质和污染物的液体样品中以简单有效的方式分离和纯化钙离子结合蛋白的方法，而没有任何预处理如加入螯合剂。更具体地，本发明涉及一种纯化钙离子结合蛋白的方法，其包括在钙离子存在下使所述蛋白质与阳离子交换载体接触以使所述蛋白质吸附到载体上，并且在洗涤后，洗脱所述蛋白质和基本上不含有通过本发明的方法获得的污染物的钙离子结合蛋白质。

2. 发明申请

US20050059805A1 Method for purifying calcium ion-binding protein 审中-公开
标题翻译：钙离子结合蛋白的纯化方法
公开(公告)号：US20050059805A1
公开(公告)日：2005-03-17
申请号：US10944810
申请日：2004-09-21
申请人： Hiroshi Mizokami , Shinichi Furukawa , Keishin Sugawara , Tatsufumi Onchi , Kazuhiro Komatsu , Satoshi Koyanagi , Hideo Yoshizaki
发明人： Hiroshi Mizokami , Shinichi Furukawa , Keishin Sugawara , Tatsufumi Onchi , Kazuhiro Komatsu , Satoshi Koyanagi , Hideo Yoshizaki
IPC分类号： C07K14/47 , C12N9/64
CPC分类号： C12N9/6432 , C07K14/4721 , C07K14/4728 , C12Y304/21006
摘要： The present invention relates to a method for purifying a calcium ion-binding protein by cation exchange chromatography. The present invention provide a method for isolating and purifying a calcium ion-binding protein in a simple and efficient manner from a liquid sample containing a calcium ion-binding protein and contaminants without any pretreatment such as addition of a chelating agent. More specifically, the present invention relates to a method for purifying a calcium ion-binding protein which comprises contacting said protein with a cation exchange carrier in the presence of calcium ions to let the said protein be adsorbed to the carrier, and after washing, eluting said protein, and to a calcium ion-binding protein having substantially no contaminants obtained by the method of the present invention.
摘要翻译：本发明涉及通过阳离子交换层析纯化钙离子结合蛋白的方法。本发明提供了从含有钙离子结合蛋白质和污染物的液体样品中以简单有效的方式分离和纯化钙离子结合蛋白的方法，而没有任何预处理如加入螯合剂。更具体地，本发明涉及一种纯化钙离子结合蛋白的方法，其包括在钙离子存在下使所述蛋白质与阳离子交换载体接触以使所述蛋白质吸附到载体上，并且在洗涤后，洗脱所述蛋白质和基本上不含有通过本发明的方法获得的污染物的钙离子结合蛋白质。

3. 发明申请

US20070065906A1 Process for producing heterologous protein in e. coli 审中-公开
标题翻译：在e。中生产异源蛋白的方法。大肠杆菌
公开(公告)号：US20070065906A1
公开(公告)日：2007-03-22
申请号：US10547802
申请日：2004-02-20
申请人： Satoshi Koyanagi , Keishin Sugawara , Yoshinobu Miyatsu , Toshio Murakami , Toshihiro Maeda , Hiroshi Mizokami
发明人： Satoshi Koyanagi , Keishin Sugawara , Yoshinobu Miyatsu , Toshio Murakami , Toshihiro Maeda , Hiroshi Mizokami
IPC分类号： C12P21/06 , C07H21/04 , C07K14/435 , C07K14/195 , C12N1/21 , C12N15/74
CPC分类号： C07K14/43531 , C12N15/70 , C12P21/02
摘要： A heterologous protein free from an inducer and a method for producing said protein are provided. A method for producing a heterologous protein which comprises the step of optionally culturing at low temperature recombinant E. coli cells expressing a heterologous protein under control of a promoter capable of inducing expression through temperature shift and then culturing at high temperature said recombinant E. coli cells in the absence of an inducer to thereby allow for expression of said heterologous protein, or the step of culturing at high temperature said recombinant E. coli cells to thereby simultaneously allow for both cell proliferation and expression of said heterologous protein, and the heterologous protein obtained by said method that is free from an inducer. Such heterologous protein may include a major mite allergen, a secretary macrophage toxin from Actinobacillus pleuropneumoniae and a surface protective antigen (SpaA) of Erysipelothrix rhusiopathiae.
摘要翻译：提供了不含诱导物的异源蛋白质及其制备方法。一种产生异源蛋白质的方法，其包括在低温下表达异源蛋白质的重组大肠杆菌细胞的步骤，所述重组大肠杆菌细胞在能够通过温度转移诱导表达并且然后在高温下培养的启动子控制下，所述重组大肠杆菌细胞在不存在诱导剂从而允许所述异源蛋白质的表达的情况下，或在高温下培养所述重组大肠杆菌细胞从而同时允许所述异源蛋白质的细胞增殖和表达的步骤，获得的异源蛋白质通过所述方法不含诱导剂。这种异源蛋白可以包括主要的螨变应原，来自胸膜肺炎放线杆菌的秘密巨噬细胞毒素和Erysipelothrix rhusiopathiae的表面保护性抗原（SpaA）。

4. 发明授权

US07667009B2 Method for purifying modified major mite allergen 失效
标题翻译：改性大螨过敏原的纯化方法
公开(公告)号：US07667009B2
公开(公告)日：2010-02-23
申请号：US10555883
申请日：2004-04-14
申请人： Satoshi Koyanagi , Kenjiro Kawatsu , Toshio Murakami , Yoshinobu Miyatsu , Toshihiro Maeda , Hiroshi Mizokami
发明人： Satoshi Koyanagi , Kenjiro Kawatsu , Toshio Murakami , Yoshinobu Miyatsu , Toshihiro Maeda , Hiroshi Mizokami
IPC分类号： A23J1/00
CPC分类号： C07K14/43531 , A61K38/00 , A61K39/00
摘要： A method for purifying a modified major mite allergen obtained by the genetic recombination technique and a purified modified major mite allergen obtained by said method for purification are provided. A method for purifying a modified major mite allergen obtained by the genetic recombination technique, which comprises the purification steps: (1) Washing and recovering inclusion bodies containing a modified major mite allergen obtained by the genetic recombination technique with MF membrane; (2) Dissolving said inclusion bodies followed by refolding; (3) Concentrating a solution containing the modified major mite allergen with simultaneous removal of low molecular weight components with ultrafiltration membrane; (4) Recovering the modified major mite allergen in non-adsorbed fractions with an anion exchanger; (5) Recovering the modified major mite allergen in adsorbed fractions with a hydrophobic gel; and (6) Recovering the modified major mite allergen in adsorbed fractions with an anion exchanger, and a modified major mite allergen with high purity obtained by said method for purification.
摘要翻译：提供了通过遗传重组技术获得的改良的主要螨变应原的纯化方法和通过所述纯化方法获得的纯化的改良的主要螨变应原。一种用于纯化通过遗传重组技术获得的改性主要螨变应原的方法，其包括纯化步骤：（1）洗涤并回收含有通过MF膜的遗传重组技术获得的修饰的主要螨变应原的包含体; （2）溶解所述包涵体，然后重折叠; （3）浓缩含有改性主要螨变应原的溶液，同时用超滤膜除去低分子量成分; （4）用阴离子交换剂回收未吸附级分的改性主要螨变应原; （5）用疏水凝胶回收吸附级分中的改性主要螨变应原; 和（6）用阴离子交换剂回收吸附级分中的改性主要螨变应原，以及通过所述纯化方法获得的具有高纯度的改性主要螨变应原。

5. 发明申请

US20080113424A1 Method for Purifying Modified Major Mite Allergen 失效
标题翻译：纯化改良的主要螨过敏原的方法
公开(公告)号：US20080113424A1
公开(公告)日：2008-05-15
申请号：US10555883
申请日：2004-04-14
申请人： Satoshi Koyanagi , Kenjiro Kawatsu , Toshio Murakami , Yoshinobu Miyatsu , Toshihiro Maeda , Hiroshi Mizokami
发明人： Satoshi Koyanagi , Kenjiro Kawatsu , Toshio Murakami , Yoshinobu Miyatsu , Toshihiro Maeda , Hiroshi Mizokami
IPC分类号： C07K1/00 , C07K16/00
CPC分类号： C07K14/43531 , A61K38/00 , A61K39/00
摘要： A method for purifying a modified major mite allergen obtained by the genetic recombination technique and a purified modified major mite allergen obtained by said method for purification are provided. A method for purifying a modified major mite allergen obtained by the genetic recombination technique, which comprises the purification steps: (1) Washing and recovering inclusion bodies containing a modified major mite allergen obtained by the genetic recombination technique with MF membrane; (2) Dissolving said inclusion bodies followed by refolding; (3) Concentrating a solution containing the modified major mite allergen with simultaneous removal of low molecular weight components with ultrafiltration membrane; (4) Recovering the modified major mite allergen in non-adsorbed fractions with an anion exchanger; (5) Recovering the modified major mite allergen in adsorbed fractions with a hydrophobic gel; and (6) Recovering the modified major mite allergen in adsorbed fractions with an anion exchanger, and a modified major mite allergen with high purity obtained by said method for purification.
摘要翻译：提供了通过遗传重组技术获得的改良的主要螨变应原的纯化方法和通过所述纯化方法获得的纯化的改良的主要螨变应原。一种用于纯化通过遗传重组技术获得的改良的主要螨变应原的方法，其包括纯化步骤：（1）洗涤并回收含有通过MF膜的遗传重组技术获得的改性主要螨变应原的包含体; （2）溶解所述包涵体，然后重折叠; （3）浓缩含有改性主要螨变应原的溶液，同时用超滤膜除去低分子量成分; （4）用阴离子交换剂回收未吸附级分的改性主要螨变应原; （5）用疏水凝胶回收吸附级分中的改性主要螨变应原; 和（6）用阴离子交换剂回收吸附级分中的改性主要螨变应原，以及通过所述纯化方法获得的具有高纯度的改性主要螨变应原。

6. 发明授权

US06870871B2 Semiconductor laser devices, and semiconductor laser modules and optical communication systems using the same 有权
标题翻译：半导体激光器件和半导体激光器模块以及使用其的光通信系统
公开(公告)号：US06870871B2
公开(公告)日：2005-03-22
申请号：US10001057
申请日：2001-10-31
申请人： Junji Yoshida , Naoki Tsukiji , Takeshi Aikiyo , Satoshi Koyanagi
发明人： Junji Yoshida , Naoki Tsukiji , Takeshi Aikiyo , Satoshi Koyanagi
IPC分类号： H01S5/022 , H01S5/14 , H01S5/00
CPC分类号： H01S5/0287 , H01S5/02208 , H01S5/02284 , H01S5/1039 , H01S5/146
摘要： A semiconductor laser device and module for use in a dense wavelength division multiplexed optical communications system are shown. The laser device preferably has cavity lengths greater than 1000 μm, and compressive strain multi-quantum well active layer, and front-facet reflectivity of less than about 4%. Higher optical outputs by longer cavity lengths are achieved. Preferred modules use these laser diodes with external wavelength-selective reflectors that have narrow bandwidths of 3 nm or less, and which include a plurality of longitudinal mode subpeaks within the bandwidth. Relationships between reflectivity value of the front facet and the peak reflectivity of the wavelength-selective reflector for long cavity length laser device are also disclosed, with the relationships providing higher output power along with a stabilized output spectrum.
摘要翻译：示出了用于密集波分复用光通信系统的半导体激光器件和模块。激光装置优选具有大于1000μm的空腔长度和压缩应变多量子阱活性层，并且前端反射率小于约4％。实现了更长的腔体长度的较高的光输出。优选的模块将这些激光二极管与具有3nm或更小的窄带宽的外部波长选择性反射器一起使用，并且其包括带宽内的多个纵向模式子峰。还公开了用于长腔激光器件的前视面的反射率值与波长选择反射器的峰值反射率之间的关系，其中提供更高的输出功率以及稳定的输出光谱。

7. 发明授权

US06614822B2 Semiconductor laser devices, and semiconductor laser modules and optical communication systems using the same 有权
标题翻译：半导体激光器件和半导体激光器模块以及使用其的光通信系统
公开(公告)号：US06614822B2
公开(公告)日：2003-09-02
申请号：US09776226
申请日：2001-02-02
申请人： Junji Yoshida , Naoki Tsukiji , Takeshi Aikiyo , Satoshi Koyanagi
发明人： Junji Yoshida , Naoki Tsukiji , Takeshi Aikiyo , Satoshi Koyanagi
IPC分类号： H01S500
CPC分类号： H01S5/146 , H01S3/094069 , H01S3/302 , H01S5/005 , H01S5/02284 , H01S5/028 , H01S5/0287 , H01S5/1039 , H01S5/227
摘要： A semiconductor laser device and module for use in a dense wavelength division multiplexed optical communications system are shown. The laser device preferably has cavity lengths greater than 1000 &mgr;m, and compressive strain multi-quantum well active layer, and front-facet reflectivity of less than about 4%. Higher optical outputs and longer cavity lengths are achieved. Preferred modules use these laser diodes with external wavelength-selective reflectors that have narrow bandwidths of 3 nm or less, and which include a plurality of longitudinal mode subpeaks within the bandwidth. Relationships between reflectivity value of the front facet and the peak reflectivity of the wavelength-selective reflector for long cavity length laser device are also disclosed, with the relationships providing higher output power along with a stabilized output spectrum.
摘要翻译：示出了用于密集波分复用光通信系统的半导体激光器件和模块。激光装置优选具有大于1000μm的空腔长度和压缩应变多量子阱活性层，并且前端反射率小于约4％。实现更高的光输出和更长的腔长度。优选的模块将这些激光二极管与具有3nm或更小的窄带宽的外部波长选择性反射器一起使用，并且其包括带宽内的多个纵向模式子峰。还公开了用于长腔激光器件的前视面的反射率值与波长选择反射器的峰值反射率之间的关系，其中提供更高的输出功率以及稳定的输出光谱。

8. 发明授权

US06343088B1 Semiconductor laser module 有权
标题翻译：半导体激光模块
公开(公告)号：US06343088B1
公开(公告)日：2002-01-29
申请号：US09381120
申请日：1999-09-15
申请人： Akira Mugino , Satoshi Koyanagi , Takeo Shimizu
发明人： Akira Mugino , Satoshi Koyanagi , Takeo Shimizu
IPC分类号： H01S500
CPC分类号： H01S5/028 , H01S5/0281 , H01S5/0287 , H01S5/141 , H01S5/146
摘要： A semiconductor laser module 1 comprising a semiconductor laser device 2 having an emitting surface 2a from which excited light is emitted and a reflecting surface 2b opposite to the emitting surface, and an optical feedback medium 3 for feeding most of optical power emitted from the emitting surface 2a of the semiconductor laser device 2 back to the semiconductor laser device 2 by coupling means 4 and emitting part of the optical power. The semiconductor laser device 2 has a low reflectance multilayer coating 2c formed on the emitting surface 2a and having a reflectance of 10−4 to 10%. The low reflectance multilayer coating 2c has a reflection spectrum in the form of a curve having a maximum value at the central wavelength of reflection and minimum values on both sides of the central wavelength of reflection.
摘要翻译：一种半导体激光器模块1，包括具有发射激发光的发射表面2a和与发射表面相对的反射表面2b的半导体激光器件2和用于馈送从发射表面发射的大部分光功率的光反馈介质3 半导体激光装置2的2a通过耦合装置4返回到半导体激光装置2并发射光功率的一部分。半导体激光装置2具有形成在发光面2a上的反射率为10 -4〜10％的低反射率多层膜2c。低反射率多层涂层2c具有在反射的中心波长处具有最大值的曲线形式的反射光谱，并且在中心反射波长的两侧具有最小值。

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