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1. 发明授权

US06727411B2 Method of producing transgenic plants having improved amino acid composition 失效
标题翻译：生产具有改进的氨基酸组成的转基因植物的方法
公开(公告)号：US06727411B2
公开(公告)日：2004-04-27
申请号：US09727758
申请日：2000-12-04
申请人： Hiroaki Kisaka , Takao Kida
发明人： Hiroaki Kisaka , Takao Kida
IPC分类号： C12N1529
CPC分类号： C12N9/88 , C12N15/8251 , C12N15/8253
摘要： Transgenic plants containing free amino acids, particularly at least one amino acid selected from among glutamic acid, asparagine, aspartic acid, serine, threonine, alanine and histidine accumulated in a large amount, in edible parts thereof, and a method of producing them are provided. In this method, glutamate decarboxylase(GAD) gene is introduced into a plant in anti-sense direction together with an appropriate regulatory sequence, and the expression of GAD gene is supressed.
摘要翻译：提供含有游离氨基酸，特别是选自谷氨酸，天冬酰胺，天冬氨酸，丝氨酸，苏氨酸，丙氨酸和组氨酸中的至少一种氨基酸的大量植物，其可食用部分的转基因植物及其制备方法在该方法中，将谷氨酸脱羧酶（GAD）基因连同适当的调节序列一起引入反义方向的植物中，并且抑制GAD基因的表达。

2. 发明授权

US06969782B2 Method of producing transgenic plants having improved amino acid composition 失效
标题翻译：生产具有改进的氨基酸组成的转基因植物的方法
公开(公告)号：US06969782B2
公开(公告)日：2005-11-29
申请号：US09729821
申请日：2000-12-06
申请人： Hiroaki Kisaka , Takao Kida
发明人： Hiroaki Kisaka , Takao Kida
IPC分类号： C12N9/00 , C12N9/02 , C12N15/53 , C12N15/82 , C12N15/83 , C12N15/90 , A01H5/00 , A01H5/10
CPC分类号： C12N9/0004 , C12N9/0016 , C12N9/93 , C12N15/8241 , C12N15/8251 , C12N15/8261 , C12Y104/01002 , C12Y104/01004 , Y02A40/146
摘要： Transgenic plants containing free amino acids, particularly at least one amino acid selected from among glutamic acid, asparagine, aspartic acid, serine, threonine, alanine and histidine accumulated in a large amount, in edible parts thereof, and a method of producing them are provided. In this method, glutamate dehydrogenase (GDH) gene is introduced into a plant together with a regulator sequence suitable for over expressing the sequence encoding GDH gene in plant cells.
摘要翻译：提供含有游离氨基酸，特别是选自谷氨酸，天冬酰胺，天冬氨酸，丝氨酸，苏氨酸，丙氨酸和组氨酸中的至少一种氨基酸的大量植物，其可食用部分的转基因植物及其制备方法。在该方法中，将谷氨酸脱氢酶（GDH）基因与适于过度表达植物细胞中编码GDH基因的序列的调节序列一起引入植物。

3. 发明申请

US20070130643A1 METHOD OF PRODUCING TRANSGENIC PLANTS HAVING IMPROVED AMINO ACID COMPOSITION AND IMPROVED YIELDING 审中-公开
标题翻译：生产具有改进的氨基酸组成的转基因植物的方法和改进的接种
公开(公告)号：US20070130643A1
公开(公告)日：2007-06-07
申请号：US11626131
申请日：2007-01-23
申请人： Hiroaki KISAKA , Takao Kida
发明人： Hiroaki KISAKA , Takao Kida
IPC分类号： A01H5/00 , A01H1/00
CPC分类号： C12N9/0004 , C12N9/0016 , C12N9/93 , C12N15/8241 , C12N15/8251 , C12N15/8261 , C12Y104/01002 , C12Y104/01004 , Y02A40/146
摘要： The object of the present invention is to provide transgenic plants which accumulate free amino acids, particularly at least one amino acid selected from among glutamic acid, asparagine, aspartic acid, serine, threonine, alanine and histidine accumulated in a large amount, in the edible parts thereof, and a method of producing them are provided. The other object of the present invention is to provide a method of increasing the yielding of potato and to provide a transgenic potato of which yielding can be increased. In the present invention, a sequence encoding glutamate dehydrogenase (GDH) gene is introduced into a plant together with a suitable regulatory sequence to express it in a plant cell, and thereby the GDH gene is excessively expressed.
摘要翻译：本发明的目的是提供一种转基因植物，其在食用中积累游离氨基酸，特别是至少一种选自谷氨酸，天冬酰胺，天冬氨酸，丝氨酸，苏氨酸，丙氨酸和组氨酸中的至少一种氨基酸部分，以及它们的制造方法。本发明的另一个目的是提供一种增加马铃薯产量的方法，并且提供可以提高产量的转基因马铃薯。在本发明中，将编码谷氨酸脱氢酶（GDH）基因的序列与合适的调节序列一起导入植物，以在植物细胞中表达，从而过度表达GDH基因。

4. 发明申请

US20050066391A1 Methods of producing nucleic acids coding for proteins have raffinose synthase activity 审中-公开
标题翻译：编码蛋白质的核酸的方法具有棉子糖合酶活性
公开(公告)号：US20050066391A1
公开(公告)日：2005-03-24
申请号：US10948251
申请日：2004-09-24
申请人： Chieko Osumi , Jinshi Nozaki , Takao Kida
发明人： Chieko Osumi , Jinshi Nozaki , Takao Kida
IPC分类号： C12N9/10 , C12N15/82 , A01H1/00 , C07H21/04 , C12N5/04 , C12N9/24
CPC分类号： C12N9/1051 , C12N15/8245 , C12N15/8246
摘要： Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol: (1) action and substrate specificity: produces raffinose from sucrose and galactinol; (2) optimum pH: about 6 to 8; (3) optimum temperature: about 35 to 40° C.; (4) molecular weight: (i) about 75 kDa to 95 kDa estimated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and (iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.
摘要翻译：通过使具有以下性质的棉子糖合成酶作用于蔗糖和半乳聚糖来制备棉子糖：（1）作用和底物特异性：从蔗糖和肌醇半乳糖苷产生棉子糖; （2）最适pH：约6〜8; （3）最适温度：约35〜40℃。（4）分子量：（i）通过凝胶过滤色谱法估计约75kDa至95kDa; （ii）通过聚丙烯酰胺凝胶电泳（Native PAGE）估计约90kDa至100kDa; 和（iii）在还原条件（SDS-PAGE）下通过SDS-聚丙烯酰胺凝胶电泳估计约90kDa至100kDa; 和（5）抑制：被碘乙酰胺，N-乙基马来酰亚胺和肌醇肌醇抑制。

5. 发明申请

US20050009165A1 Raffinose synthase gene, method for producing raffinose, and transgenic plant 审中-公开
标题翻译：棉子糖合成酶基因，棉子糖生产方法和转基因植物
公开(公告)号：US20050009165A1
公开(公告)日：2005-01-13
申请号：US10921994
申请日：2004-08-20
申请人： Chieko Osumi , Jinshi Nozaki , Takao Kida
发明人： Chieko Osumi , Jinshi Nozaki , Takao Kida
IPC分类号： C12N9/10 , C12N15/29 , C12N15/82 , C07H21/04 , C08B37/00 , C12N9/24 , C12P19/04
CPC分类号： C12N9/1051 , C12N15/8245 , C12N15/8246
摘要： Raffinose synthase purified from cucumber is allowed to act on sucrose and galactinol. Thus raffinose is efficiently produced. The function of endogenous raffinose synthase is regulated by transforming a plant with a chimeric gene comprising a raffinose synthase gene and a regulatory region expressible in the plant. Thus a plant, in which raffinose family oligosaccharides are decreased, is created.
摘要翻译：从黄瓜纯化的棉子糖合成酶可以作用于蔗糖和肌醇半乳糖苷。因此，有效地生产棉子糖。通过用包含棉子糖合成酶基因和在植物中可表达的调节区的嵌合基因转化植物来调节内源棉子糖合成酶的功能。因此，产生棉子糖家族寡糖减少的植物。

6. 发明授权

US4225585A Fungicidal and bactericidal compositions and method for protecting plants by use thereof 失效
标题翻译：杀真菌和杀菌组合物及其使用方法
公开(公告)号：US4225585A
公开(公告)日：1980-09-30
申请号：US908750
申请日：1978-05-23
申请人： Takao Kida , Zuisho Terahara , Toshiro Shida , Hiroshi Mizuno , Yoshiyuki Takahara , Yoshiteru Hirose
发明人： Takao Kida , Zuisho Terahara , Toshiro Shida , Hiroshi Mizuno , Yoshiyuki Takahara , Yoshiteru Hirose
IPC分类号： A01N63/02 , A01N9/00 , A61K31/71 , A61K35/00
CPC分类号： A01N63/02
摘要： Fungicidal compositions for agricultural and horticultural use are provided which contain as an active ingredient thereof the antibiotics angustmycin A or angustmycin C. They exhibit excellent protective effects against attack by plant disease fungi or bacteria, have no phytotoxicity and cause no pollution in the environment. A method for protecting plants by the use thereof is also provided.
摘要翻译：提供了农药园艺用杀真菌组合物，其中含有作为其活性成分的抗生素黄曲霉素A或angustmycin C.它们对植物病害真菌或细菌的侵袭具有优异的保护作用，无植物毒性，并且在环境中不造成污染。还提供了通过其使用来保护植物的方法。

7. 发明授权

US07455996B2 Soybean raffinose synthase and a method for producing raffinose 失效
标题翻译：大豆棉子糖合成酶和生产棉子糖的方法
公开(公告)号：US07455996B2
公开(公告)日：2008-11-25
申请号：US11034848
申请日：2005-01-14
申请人： Chieko Osumi , Jinshi Nozaki , Takao Kida
发明人： Chieko Osumi , Jinshi Nozaki , Takao Kida
IPC分类号： C12P19/18 , C12N1/20 , C12N9/10 , C07H21/04
CPC分类号： C12N9/1051 , C12N15/8245 , C12N15/8246
摘要： Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol: (1) action and substrate specificity: produces raffinose from sucrose and galactinol; (2) optimum pH: about 6 to 8; (3) optimum temperature: about 35 to 40° C.; (4) molecular weight: (i) about 75 kDa to 95 kDa estimated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and (iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.
摘要翻译：通过使具有以下性质的棉子糖合成酶作用于蔗糖和半乳聚糖来制备棉子糖：（1）作用和底物特异性：从蔗糖和肌醇半乳糖苷产生棉子糖; （2）最适pH：约6〜8; （3）最适温度：约35〜40℃。（4）分子量：（i）通过凝胶过滤色谱法估计约75kDa至95kDa; （ii）通过聚丙烯酰胺凝胶电泳（Native PAGE）估计约90kDa至100kDa; 和（iii）在还原条件（SDS-PAGE）下通过SDS-聚丙烯酰胺凝胶电泳估计约90kDa至100kDa; 和（5）抑制：被碘乙酰胺，N-乙基马来酰亚胺和肌醇肌醇抑制。

8. 发明申请

US20050160497A1 Soybean raffinose synthase and a method for producing raffinose 失效
标题翻译：大豆棉子糖合成酶和生产棉子糖的方法
公开(公告)号：US20050160497A1
公开(公告)日：2005-07-21
申请号：US11034678
申请日：2005-01-14
申请人： Chieko Osumi , Jinshi Nozaki , Takao Kida
发明人： Chieko Osumi , Jinshi Nozaki , Takao Kida
IPC分类号： C12N9/10 , C12N15/82 , A01H1/00 , C07H21/04 , C12N5/04
CPC分类号： C12N9/1051 , C12N15/8245 , C12N15/8246
摘要： Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol: (1) action and substrate specificity: produces raffinose from sucrose and galactinol; (2) optimum pH: about 6 to 8; (3) optimum temperature: about 35 to 40° C.; (4) molecular weight: (i) about 75 kDa to 95 kDa estimated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and (iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.
摘要翻译：通过使具有以下性质的棉子糖合成酶作用于蔗糖和半乳聚糖来制备棉子糖：（1）作用和底物特异性：从蔗糖和肌醇半乳糖苷产生棉子糖; （2）最适pH：约6〜8; （3）最适温度：约35〜40℃。（4）分子量：（i）通过凝胶过滤色谱法估计约75kDa至95kDa; （ii）通过聚丙烯酰胺凝胶电泳（Native PAGE）估计约90kDa至100kDa; 和（iii）在还原条件（SDS-PAGE）下通过SDS-聚丙烯酰胺凝胶电泳估计约90kDa至100kDa; 和（5）抑制：被碘乙酰胺，N-乙基马来酰亚胺和肌醇肌醇抑制。

9. 发明授权

US4642335A Anthracycline compounds bound to hydrophilic polypeptides 失效
标题翻译：结合亲水多肽的蒽环类化合物
公开(公告)号：US4642335A
公开(公告)日：1987-02-10
申请号：US738352
申请日：1985-05-28
申请人： Shigeyoshi Miyashiro , Takao Kida , Tsuyoshi Shiio , Hiroshiro Shibai
发明人： Shigeyoshi Miyashiro , Takao Kida , Tsuyoshi Shiio , Hiroshiro Shibai
IPC分类号： C07K14/195 , A61K31/70 , A61K31/7028 , A61K31/7034 , A61K31/704 , A61K35/74 , A61K38/00 , A61P35/00 , C07H15/252 , C07K14/00 , C07K14/41 , C07K17/02 , C07K17/06
CPC分类号： C07H15/252 , Y10S530/812 , Y10S530/825
摘要： This invention relates to an antitumor composition of anthracycline compounds bonded to hydrophilic polypeptides having a molecular weight between 10,000 and 15,000.
摘要翻译：本发明涉及结合至分子量为10,000至15,000的亲水性多肽的蒽环类化合物的抗肿瘤组合物。

10. 发明授权

US4560748A Streptothricin-group compounds 失效
标题翻译：链球菌素类化合物
公开(公告)号：US4560748A
公开(公告)日：1985-12-24
申请号：US500908
申请日：1983-06-03
申请人： Shigezo Udaka , Shigeyoshi Miyashiro , Kazuo Hirayama , Toshihiko Ando , Asao Murai , Tsuyoshi Shiio , Takao Kida , Hiroshiro Shibai
发明人： Shigezo Udaka , Shigeyoshi Miyashiro , Kazuo Hirayama , Toshihiko Ando , Asao Murai , Tsuyoshi Shiio , Takao Kida , Hiroshiro Shibai
IPC分类号： C12P21/02 , A61K31/70 , A61K31/7042 , A61K31/7052 , A61P31/04 , A61P31/12 , A61P35/00 , C07H17/02 , C12P19/28 , C07H17/00
CPC分类号： C12R1/465 , C07H17/02 , C12P19/28
摘要： A streptothricin-group compound having the formula: ##STR1## in which R represents ##STR2## is disclosed along with methods for producing this compound and a microorganism useful in its production.
摘要翻译：公开了具有下式的其中R表示 + TR 的下式的链霉菌素基化合物以及用于生产该化合物的方法和用于其生产的微生物。

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