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1. 发明申请

US20100330665A1 METHOD FOR PRODUCING A CELL CULTURE SUBSTRATE 审中-公开
标题翻译：生产细胞培养基质的方法
公开(公告)号：US20100330665A1
公开(公告)日：2010-12-30
申请号：US12881492
申请日：2010-09-14
申请人： Hideyuki Miyake , Hideshi Hattori , Yoichi Takahashi
发明人： Hideyuki Miyake , Hideshi Hattori , Yoichi Takahashi
IPC分类号： C12M1/26
CPC分类号： B01L3/0268 , B01L3/5085 , B01L2200/12 , B01L2300/069 , B01L2300/10 , B01L2300/1888 , B01L2400/0439 , C12M23/20 , C12M25/06 , C12N5/0068 , C12N2533/12 , C12N2535/10
摘要： A main object of the invention is to provide a new method for producing a cell culture substrate used to cause cells to adhere in a highly precise form onto a base material and then culture the cells.To attain the object, the invention provides a method for producing a cell culture substrate comprising: a patterning substrate forming process of forming, on a base material, a cell culture patterning layer wherein a cell adhesion portion having cell adhesive properties and a cell adhesion-inhibiting portion having cell adhesion-inhibiting properties can be formed by action of a photocatalyst by energy irradiation, thereby forming a patterning substrate; an energy irradiating process of irradiating the energy onto the cell culture patterning layer, thereby forming the cell adhesion portion and the cell adhesion-inhibiting portion in a pattern form by action of the photocatalyst; and a cell-containing liquid applying process of applying a cell-containing liquid onto the cell adhesion portion by a region-selecting applying method of applying the cell-containing liquid selectively onto the patterned cell adhesion portion.
摘要翻译：本发明的主要目的是提供一种用于生产细胞培养基质的新方法，该方法用于使细胞以高度精确的形式粘附到基材上，然后培养细胞。为了达到上述目的，本发明提供一种细胞培养基材的制造方法，其特征在于，包括：在基材上形成细胞培养图案形成层的图案形成基板的形成工序，其中，具有细胞粘合性的细胞粘附部，通过能量照射可以通过光催化剂的作用形成具有细胞粘附抑制特性的抑制部分，从而形成图案化衬底; 将能量照射到细胞培养图案化层上的能量照射方法，由此通过光催化剂的作用形成图案形式的细胞粘附部分和细胞粘附抑制部分; 以及通过选择性地将细胞含有液体施加到图案化细胞粘附部分上的区域选择施加方法将含细胞的液体施加到细胞粘附部分上的含细胞的液体施加方法。

2. 发明申请

US20050279730A1 Method for manufacturing cell culture substrate 失效
标题翻译：细胞培养基质的制造方法
公开(公告)号：US20050279730A1
公开(公告)日：2005-12-22
申请号：US11062674
申请日：2005-02-22
申请人： Hideyuki Miyake , Hideshi Hattori , Yoichi Takahashi
发明人： Hideyuki Miyake , Hideshi Hattori , Yoichi Takahashi
IPC分类号： B01L3/00 , B01L3/02 , B41J2/01 , B41J2/06 , C12N5/00
CPC分类号： B01L3/0268 , B01L3/5085 , B01L2200/12 , B01L2300/069 , B01L2300/10 , B01L2300/1888 , B01L2400/0439 , C12M23/20 , C12M25/06 , C12N5/0068 , C12N2533/12 , C12N2535/10
摘要： A main object of the invention is to provide a new method for producing a cell culture substrate used to cause cells to adhere in a highly precise form onto a base material and then culture the cells. To attain the object, the invention provides a method for producing a cell culture substrate comprising: a patterning substrate forming process of forming, on a base material, a cell culture patterning layer wherein a cell adhesion portion having cell adhesive properties and a cell adhesion-inhibiting portion having cell adhesion-inhibiting properties can be formed by action of a photocatalyst by energy irradiation, thereby forming a patterning substrate; an energy irradiating process of irradiating the energy onto the cell culture patterning layer, thereby forming the cell adhesion portion and the cell adhesion-inhibiting portion in a pattern form by action of the photocatalyst; and a cell-containing liquid applying process of applying a cell-containing liquid onto the cell adhesion portion by a region-selecting applying method of applying the cell-containing liquid selectively onto the patterned cell adhesion portion.
摘要翻译：本发明的主要目的是提供一种用于生产细胞培养基质的新方法，该方法用于使细胞以高度精确的形式粘附在基材上，然后培养细胞。为了达到上述目的，本发明提供一种细胞培养基材的制造方法，其特征在于，包括：在基材上形成细胞培养图案形成层的图案形成基板的形成工序，其中，具有细胞粘合性的细胞粘附部，通过能量照射可以通过光催化剂的作用形成具有细胞粘附抑制特性的抑制部分，从而形成图案化衬底; 将能量照射到细胞培养图案化层上的能量照射方法，由此通过光催化剂的作用形成图案形式的细胞粘附部分和细胞粘附抑制部分; 以及通过选择性地将细胞含有液体施加到图案化细胞粘附部分上的区域选择施加方法将含细胞的液体施加到细胞粘附部分上的含细胞的液体施加方法。

3. 发明申请

US20070243613A1 Method for producing a cell culture substrate 审中-公开
标题翻译：细胞培养基质的制造方法
公开(公告)号：US20070243613A1
公开(公告)日：2007-10-18
申请号：US11709119
申请日：2007-02-20
申请人： Hideyuki Miyake , Hideshi Hattori , Yoichi Takahashi
发明人： Hideyuki Miyake , Hideshi Hattori , Yoichi Takahashi
IPC分类号： C12N5/02 , B41J2/175
CPC分类号： B01L3/0268 , B01L3/5085 , B01L2200/12 , B01L2300/069 , B01L2300/10 , B01L2300/1888 , B01L2400/0439 , C12M23/20 , C12M25/06 , C12N5/0068 , C12N2533/12 , C12N2535/10
摘要： A main object of the invention is to provide a new method for producing a cell culture substrate used to cause cells to adhere in a highly precise form onto a base material and then culture the cells. To attain the object, the invention provides a method for producing a cell culture substrate comprising: a patterning substrate forming process of forming, on a base material, a cell culture patterning layer wherein a cell adhesion portion having cell adhesive properties and a cell adhesion-inhibiting portion having cell adhesion-inhibiting properties can be formed by action of a photocatalyst by energy irradiation, thereby forming a patterning substrate; an energy irradiating process of irradiating the energy onto the cell culture patterning layer, thereby forming the cell adhesion portion and the cell adhesion-inhibiting portion in a pattern form by action of the photocatalyst; and a cell-containing liquid applying process of applying a cell-containing liquid onto the cell adhesion portion by a region-selecting applying method of applying the cell-containing liquid selectively onto the patterned cell adhesion portion.
摘要翻译：本发明的主要目的是提供一种用于生产细胞培养基质的新方法，该方法用于使细胞以高度精确的形式粘附到基材上，然后培养细胞。为了达到上述目的，本发明提供一种细胞培养基材的制造方法，其特征在于，包括：在基材上形成细胞培养图案形成层的图案形成基板的形成工序，其中，具有细胞粘合性的细胞粘附部，通过能量照射可以通过光催化剂的作用形成具有细胞粘附抑制特性的抑制部分，从而形成图案化衬底; 将能量照射到细胞培养图案化层上的能量照射方法，由此通过光催化剂的作用形成图案形式的细胞粘附部分和细胞粘附抑制部分; 以及通过选择性地将细胞含有液体施加到图案化细胞粘附部分上的区域选择施加方法将含细胞的液体施加到细胞粘附部分上的含细胞的液体施加方法。

4. 发明授权

US08497117B2 Method for manufacturing cell culture substrate 失效
标题翻译：细胞培养基质的制造方法
公开(公告)号：US08497117B2
公开(公告)日：2013-07-30
申请号：US13032998
申请日：2011-02-23
申请人： Hideyuki Miyake , Hideshi Hattori , Yoichi Takahashi
发明人： Hideyuki Miyake , Hideshi Hattori , Yoichi Takahashi
IPC分类号： C12M1/34 , C12M1/36 , C12M1/38 , C12M3/00 , C12M1/00 , C12N5/00 , C12N5/02
CPC分类号： B01L3/0268 , B01L3/5085 , B01L2200/12 , B01L2300/069 , B01L2300/10 , B01L2300/1888 , B01L2400/0439 , C12M23/20 , C12M25/06 , C12N5/0068 , C12N2533/12 , C12N2535/10
摘要： A main object of the invention is to provide a new method for producing a cell culture substrate used to cause cells to adhere in a highly precise form onto a base material and then culture the cells.To attain the object, the invention provides a method for producing a cell culture substrate comprising: a patterning substrate forming process of forming, on a base material, a cell culture patterning layer wherein a cell adhesion portion having cell adhesive properties and a cell adhesion-inhibiting portion having cell adhesion-inhibiting properties can be formed by action of a photocatalyst by energy irradiation, thereby forming a patterning substrate; an energy irradiating process of irradiating the energy onto the cell culture patterning layer, thereby forming the cell adhesion portion and the cell adhesion-inhibiting portion in a pattern form by action of the photocatalyst; and a cell-containing liquid applying process of applying a cell-containing liquid onto the cell adhesion portion by a region-selecting applying method of applying the cell-containing liquid selectively onto the patterned cell adhesion portion.
摘要翻译：本发明的主要目的是提供一种用于生产细胞培养基质的新方法，该方法用于使细胞以高度精确的形式粘附到基材上，然后培养细胞。为了达到上述目的，本发明提供一种细胞培养基材的制造方法，其特征在于，包括：在基材上形成细胞培养图案形成层的图案形成基板的形成工序，其中，具有细胞粘合性的细胞粘附部，通过能量照射可以通过光催化剂的作用形成具有细胞粘附抑制特性的抑制部分，从而形成图案化衬底; 将能量照射到细胞培养图案化层上的能量照射方法，由此通过光催化剂的作用形成图案形式的细胞粘附部分和细胞粘附抑制部分; 以及通过选择性地将细胞含有液体施加到图案化细胞粘附部分上的区域选择施加方法将含细胞的液体施加到细胞粘附部分的含细胞的液体施加方法。

5. 发明授权

US07919305B2 Method for manufacturing cell culture substrate 失效
标题翻译：细胞培养基质的制造方法
公开(公告)号：US07919305B2
公开(公告)日：2011-04-05
申请号：US11062674
申请日：2005-02-22
申请人： Hideyuki Miyake , Hideshi Hattori , Yoichi Takahashi
发明人： Hideyuki Miyake , Hideshi Hattori , Yoichi Takahashi
IPC分类号： C12M1/34 , C12M1/36 , C12M1/38 , C12M3/00 , C12M1/00 , C12N5/00 , C12N5/02
CPC分类号： B01L3/0268 , B01L3/5085 , B01L2200/12 , B01L2300/069 , B01L2300/10 , B01L2300/1888 , B01L2400/0439 , C12M23/20 , C12M25/06 , C12N5/0068 , C12N2533/12 , C12N2535/10
摘要： A main object of the invention is to provide a new method for producing a cell culture substrate used to cause cells to adhere in a highly precise form onto a base material and then culture the cells.To attain the object, the invention provides a method for producing a cell culture substrate comprising: a patterning substrate forming process of forming, on a base material, a cell culture patterning layer wherein a cell adhesion portion having cell adhesive properties and a cell adhesion-inhibiting portion having cell adhesion-inhibiting properties can be formed by action of a photocatalyst by energy irradiation, thereby forming a patterning substrate; an energy irradiating process of irradiating the energy onto the cell culture patterning layer, thereby forming the cell adhesion portion and the cell adhesion-inhibiting portion in a pattern form by action of the photocatalyst; and a cell-containing liquid applying process of applying a cell-containing liquid onto the cell adhesion portion by a region-selecting applying method of applying the cell-containing liquid selectively onto the patterned cell adhesion portion.
摘要翻译：本发明的主要目的是提供一种用于生产细胞培养基质的新方法，该方法用于使细胞以高度精确的形式粘附到基材上，然后培养细胞。为了达到上述目的，本发明提供一种细胞培养基材的制造方法，其特征在于，包括：在基材上形成细胞培养图案形成层的图案形成基板的形成工序，其中，具有细胞粘合性的细胞粘附部，通过能量照射可以通过光催化剂的作用形成具有细胞粘附抑制特性的抑制部分，从而形成图案化衬底; 将能量照射到细胞培养图案化层上的能量照射方法，由此通过光催化剂的作用形成图案形式的细胞粘附部分和细胞粘附抑制部分; 以及通过选择性地将细胞含有液体施加到图案化细胞粘附部分上的区域选择施加方法将含细胞的液体施加到细胞粘附部分的含细胞的液体施加方法。

6. 外观设计

USD687069S1 Digital video disc player 有权
公开(公告)号：USD687069S1
公开(公告)日：2013-07-30
申请号：US29401899
申请日：2011-09-16
申请人： Yoichi Takahashi , Kosaku Wada
设计人： Yoichi Takahashi , Kosaku Wada

7. 发明授权

US07856307B2 Fuel injection control device and method for multi-fuel engine 有权
标题翻译：多燃料发动机燃油喷射控制装置及方法
公开(公告)号：US07856307B2
公开(公告)日：2010-12-21
申请号：US12562135
申请日：2009-09-18
申请人： Atsushi Ito , Yoichi Takahashi , Hideya Horie
发明人： Atsushi Ito , Yoichi Takahashi , Hideya Horie
IPC分类号： F02D41/30
CPC分类号： F02D19/12 , F02D41/0025 , F02D41/0235 , F02D41/06 , F02D41/1454 , F02D41/2451 , F02D2200/0612 , F02M25/14
摘要： A fuel injection control device for a multi-fuel engine includes a corrector and a reviewer. An alcohol concentration learning unit is provided to learn an alcohol concentration in an injected fuel based on an oxygen concentration detected in an exhaust gas. A fuel injection amount controller is provided to control a fuel injection amount based on a learning value corresponding to the oxygen concentration. The corrector is configured to reduce the fuel injection amount corresponding to the learning value for a predetermined time period when the learning value is higher than a threshold value when an engine is started. The reviewer is configured to review the learning value to provide a revised learning value based on the oxygen concentration while the corrector reduces the fuel injection amount. The fuel injection amount is controlled based on the revised learning value after the corrector reduces the fuel injection amount.
摘要翻译：一种用于多燃料发动机的燃料喷射控制装置，包括校正器和检查器。提供了一种酒精浓度学习单元，用于基于在废气中检测到的氧浓度来了解喷射的燃料中的酒精浓度。提供燃料喷射量控制器，用于基于与氧气浓度对应的学习值来控制燃料喷射量。校正器被配置为当发动机起动时学习值高于阈值时，将与学习值对应的燃料喷射量减小预定时间段。审查者被配置为审查学习值以提供基于氧浓度的修正学习值，同时校正器减少燃料喷射量。在校正器减少燃料喷射量之后，基于修正后的学习值来控制燃料喷射量。

8. 发明申请

US20090248279A1 Fuel injection control apparatus and method for a multi-fuel engine, and engine incorporating same 有权
标题翻译：用于多燃料发动机的燃料喷射控制装置和方法，以及包含其的发动机
公开(公告)号：US20090248279A1
公开(公告)日：2009-10-01
申请号：US12378686
申请日：2009-02-18
申请人： Atsushi Ito , Yoichi Takahashi
发明人： Atsushi Ito , Yoichi Takahashi
IPC分类号： F02D41/30
CPC分类号： F02D41/0025 , F02D19/061 , F02D19/084 , F02D19/088 , F02D41/1454 , F02D2200/023 , F02D2200/0404 , Y02T10/36
摘要： A fuel injection control apparatus for a multi-fuel engine can optimize a fuel injection quantity, using only one basic injection map, irrespective of alcohol concentration of a fuel. An E-concentration determining apparatus determines alcohol concentration of a fuel, based on a measured oxygen amount in exhaust. Basic injection quantities are stored in a basic injection map. E-concentration coefficients are stored in an E-concentration coefficient table for a plurality of alcohol concentrations in the fuel. A basic injection quantity, corresponding to a current rotary engine speed and a throttle opening, is selected from the basic injection map. A concentration coefficient, corresponding to the alcohol concentration and the basic injection quantity, is selected from the E-concentration coefficient table. A fuel injection quantity calculator determines an applied fuel injection quantity by multiplying the basic injection quantity by the concentration coefficient. Further adjustments using a number of additional correction coefficients may be made, as needed.
摘要翻译：不管燃料的酒精浓度如何，用于多燃料发动机的燃料喷射控制装置可以仅使用一个基本喷射图来优化燃料喷射量。电离浓度测定装置基于排气中测定的氧量来确定燃料的醇浓度。基本注射量存储在基本注射图中。 E浓度系数存储在燃料中的多个醇浓度的E浓度系数表中。从基本注入图中选择对应于当前旋转发动机转速和节气门开度的基本喷射量。从E浓度系数表中选出与酒精浓度和碱性喷射量对应的浓度系数。燃料喷射量计算器通过将基本喷射量乘以浓度系数来确定施加的燃料喷射量。可以根据需要进行使用多个附加校正系数的进一步调整。

9. 外观设计

USD422998S Digital video disc player 失效
公开(公告)号：USD422998S
公开(公告)日：2000-04-18
申请号：US110479
申请日：1999-09-08
申请人： Yutaka Negishi , Yoichi Takahashi
设计人： Yutaka Negishi , Yoichi Takahashi

10. 发明授权

US5909903A Coupling structure for coupling two pipes 失效
标题翻译：用于连接两根管道的联轴器结构
公开(公告)号：US5909903A
公开(公告)日：1999-06-08
申请号：US821776
申请日：1997-03-20
申请人： Yoichi Takahashi , Eizo Suyama
发明人： Yoichi Takahashi , Eizo Suyama
IPC分类号： F16L23/04 , F16L13/14 , F16L21/00
CPC分类号： F16L13/141 , F01N13/1805 , F01N13/1855 , F01N13/1872 , F16L23/04
摘要： For coupling first and second metal pipes, a coupling structure is provided which comprises a first diametrically angled end portion of the first metal pipe and a second diametrically enlarged end portion of the second metal pipe. The second enlarged end portion is mated with the first enlarged end portion so that the first and second metal pipes are aligned. A collar member of metal is concentrically disposed within the mated first and second enlarged end portions. The collar member is formed, around an axially middle portion thereof, with an annular ridge which entirely and intimately contacts with inner surfaces of the mated first and second enlarged end portions. A fastening ring of metal is concentrically disposed about the mated first and second enlarged end portions. The fastening ring is so shaped as to have a concave inner surface which intimately and entirely contacts with rounded outer surfaces of the mated first and second enlarged end portions.
摘要翻译：为了联接第一和第二金属管，提供了一种联接结构，其包括第一金属管的第一直径成角度的端部部分和第二金属管道的第二直径扩大的端部。第二扩大端部与第一扩大端部配合，使得第一和第二金属管对齐。金属的轴环构件同心地设置在配合的第一和第二扩大端部内。环形构件围绕其轴向中间部分形成有与配合的第一和第二扩大端部的内表面完全紧密接触的环形脊。金属的紧固环围绕配合的第一和第二扩大端部同心地设置。紧固环的形状为具有与配合的第一和第二扩大端部的圆形外表面紧密且完全接触的凹形内表面。

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