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1. 发明授权

US07486886B2 Photo-micrographing device and its control method 有权
标题翻译：照相显影装置及其控制方法
公开(公告)号：US07486886B2
公开(公告)日：2009-02-03
申请号：US11330874
申请日：2006-01-11
申请人： Hideaki Endo , Akitsugu Kagayama , Katsuyoshi Yamaguchi
发明人： Hideaki Endo , Akitsugu Kagayama , Katsuyoshi Yamaguchi
IPC分类号： G02B21/36
CPC分类号： G02B21/367 , G02B21/26
摘要： A photo-micrographing device includes a microscope, a unit for obtaining an image of an inspection object, a unit for setting a plurality of view fields of the microscope and storing position information of a stage corresponding to each view field, a unit for storing setting information of the microscope and a camera unit in each view field, a unit for storing shooting conditions corresponding to each view field where time-lapse shooting is applied, a unit for obtaining a time-lapse shooting image for each view field where the time-lapse shooting is applied, based on the stored position information, the stored setting information and the stored shooting conditions, and a unit for collectively displaying latest taken time-lapse shooting images for each view field where the time-lapse shooting is applied.
摘要翻译：照相显影装置包括显微镜，用于获得检查对象的图像的单元，用于设置显微镜的多个视场的单元，并存储与每个视野相对应的台的位置信息，用于存储设置的单元每个视野中的显微镜和相机单元的信息，用于存储与应用了延时拍摄的每个视野相对应的拍摄条件的单元，用于获得每个视场的延时拍摄图像的单元，基于所存储的位置信息，存储的设置信息和存储的拍摄条件来应用经过拍摄，以及用于对应用延时拍摄的每个视野进行集体显示最新拍摄的延时拍摄图像的单元。

2. 发明申请

US20060141609A1 Culture observation apparatus, sample tray heat-insulating device and lid 有权
标题翻译：文化观察装置，样品托盘隔热装置和盖子
公开(公告)号：US20060141609A1
公开(公告)日：2006-06-29
申请号：US11312114
申请日：2005-12-20
申请人： Akitsugu Kagayama , Ryuichi Hirano , Atsuhiro Tsuchiya , Kenichi Koyama , Kazuhiro Hasegawa , Katsuyoshi Yamaguchi , Hideaki Endo
发明人： Akitsugu Kagayama , Ryuichi Hirano , Atsuhiro Tsuchiya , Kenichi Koyama , Kazuhiro Hasegawa , Katsuyoshi Yamaguchi , Hideaki Endo
IPC分类号： C12M1/34
CPC分类号： C12M23/48 , C12M23/50 , C12M41/12
摘要： A culture observation apparatus, which is used for observing a cultured cell while culturing the cell, includes a culture device that forms a culture space which is controlled to be an environment suitable for culturing the cultured cell; a sample tray that holds a container housing the cultured cell and a culture medium; a microscope that serves for observing the cultured cell; a tray holding mechanism that holds the sample tray in the culture space in a detachable manner with good reproducibility; and a shifting mechanism that relatively shifts the sample tray held by the tray holding mechanism and a light axis of the microscope along a plane that is orthogonal to the light axis, wherein the sample tray has a heater used for heating the sample tray, and is electrically connectable to an energy supplying unit that supplies energy to the sample tray.
摘要翻译：用于在培养细胞时观察培养细胞的培养观察装置包括形成培养空间的培养装置，该培养装置被控制为适于培养培养细胞的环境; 容纳容纳培养细胞的容器和培养基的样品盘; 用于观察培养细胞的显微镜; 托盘保持机构，以可拆卸的方式将样品盘保持在培养空间中，具有良好的再现性; 以及移动机构，其使由所述托盘保持机构保持的所述检体托盘和所述显微镜的光轴沿着与所述光轴正交的平面相对移动，所述检体托盘具有用于加热所述检体托盘的加热器，电连接到向样品托盘供应能量的能量供应单元。

3. 发明授权

US07816126B2 Culture observation apparatus, sample tray heat-insulating device and lid 有权
标题翻译：文化观察装置，样品托盘隔热装置和盖子
公开(公告)号：US07816126B2
公开(公告)日：2010-10-19
申请号：US11312114
申请日：2005-12-20
申请人： Akitsugu Kagayama , Ryuichi Hirano , Atsuhiro Tsuchiya , Kenichi Koyama , Kazuhiro Hasegawa , Katsuyoshi Yamaguchi , Hideaki Endo
发明人： Akitsugu Kagayama , Ryuichi Hirano , Atsuhiro Tsuchiya , Kenichi Koyama , Kazuhiro Hasegawa , Katsuyoshi Yamaguchi , Hideaki Endo
IPC分类号： C12M3/00 , C12M1/34
CPC分类号： C12M23/48 , C12M23/50 , C12M41/12
摘要： A culture observation apparatus, which is used for observing a cultured cell while culturing the cell, includes a culture device that forms a culture space which is controlled to be an environment suitable for culturing the cultured cell; a sample tray that holds a container housing the cultured cell and a culture medium; a microscope that serves for observing the cultured cell; a tray holding mechanism that holds the sample tray in the culture space in a detachable manner with good reproducibility; and a shifting mechanism that relatively shifts the sample tray held by the tray holding mechanism and a light axis of the microscope along a plane that is orthogonal to the light axis, wherein the sample tray has a heater used for heating the sample tray, and is electrically connectable to an energy supplying unit that supplies energy to the sample tray.
摘要翻译：用于在培养细胞时观察培养细胞的培养观察装置包括形成培养空间的培养装置，该培养装置被控制为适于培养培养细胞的环境; 容纳容纳培养细胞的容器和培养基的样品盘; 用于观察培养细胞的显微镜; 托盘保持机构，以可拆卸的方式将样品盘保持在培养空间中，具有良好的再现性; 以及移动机构，其使由所述托盘保持机构保持的所述检体托盘和所述显微镜的光轴沿着与所述光轴正交的平面相对移动，所述检体托盘具有用于加热所述检体托盘的加热器，电连接到向样品托盘供应能量的能量供应单元。

4. 发明授权

US07595188B2 Observation apparatus 有权
标题翻译：观察装置
公开(公告)号：US07595188B2
公开(公告)日：2009-09-29
申请号：US11302591
申请日：2005-12-12
申请人： Kazuhiro Hasegawa , Akitsugu Kagayama , Hideaki Endo , Ryuichi Hirano , Atsuhiro Tsuchiya , Kenichi Koyama , Katsuyoshi Yamaguchi
发明人： Kazuhiro Hasegawa , Akitsugu Kagayama , Hideaki Endo , Ryuichi Hirano , Atsuhiro Tsuchiya , Kenichi Koyama , Katsuyoshi Yamaguchi
IPC分类号： C12M3/00
CPC分类号： C12M23/50 , C12M23/10 , C12M23/48 , C12M41/36
摘要： An observation apparatus includes a sample tray that holds a container housing a cultured cell and a culture medium and covered with a lid; an observation unit that serves for observation of the cultured cell; and a shifting unit that relatively shifts the sample tray and a light axis of the observation unit along a plane that is orthogonal to the light axis of the observation unit, wherein the sample tray includes a container holding unit that holds the container by utilizing an elastic force, and at least one of the lid and one portion of the lid is made detachable, with the container being held in the sample tray by the container holding unit.
摘要翻译：观察装置包括：样本托盘，其容纳容纳培养细胞和培养基并容纳盖子的容器; 用于观察培养细胞的观察单元; 以及移动单元，其沿着与所述观察单元的光轴正交的平面相对地移动所述样本托盘和所述观察单元的光轴，其中，所述样本托盘包括容器保持单元，所述容器保持单元通过利用弹性并且盖子和盖子的一部分中的至少一个是可拆卸的，容器通过容器保持单元保持在样品盘中。

5. 发明授权

US07382531B2 Microscope and method of preventing dew condensation on objective lens 有权
标题翻译：显微镜及防止物镜结露的方法
公开(公告)号：US07382531B2
公开(公告)日：2008-06-03
申请号：US11259392
申请日：2005-10-26
申请人： Atsuhiro Tsuchiya , Katsuyoshi Yamaguchi , Akitsugu Kagayama , Kazuhiro Hasegawa , Hideaki Endo , Kenichi Koyama
发明人： Atsuhiro Tsuchiya , Katsuyoshi Yamaguchi , Akitsugu Kagayama , Kazuhiro Hasegawa , Hideaki Endo , Kenichi Koyama
IPC分类号： G02B21/26
CPC分类号： G02B27/0006 , C12M41/12 , C12M41/36 , G02B7/008 , G02B21/30
摘要： The problem to be solved by the present invention is to provide a microscope that prevents a dew condensation on an objective lens and allows obtaining a good microscopic image, and a method of preventing a dew condensation on an objective lens.A microscope includes an objective lens having a heater 22 in a cell incubator 3 having a high humidity space. The high humidity space is maintained at 90% to 100% of relative humidity and controlled to be at a predetermined gas temperature. A cultured cell 12 in the cell incubator 3 is observed via the objective lens 1, and the heater 22 is controlled to make the temperature of the objective lens 1 higher than the gas temperature of the high humidity space.
摘要翻译：本发明要解决的问题是提供一种防止物镜上的结露并且可以获得良好的显微图像的显微镜，以及防止物镜上的结露的方法。显微镜包括在具有高湿度空间的细胞培养器3中具有加热器22的物镜。高湿度空间保持在相对湿度的90％至100％，并被控制在预定的气体温度。通过物镜1观察细胞培养器3中的培养细胞12，控制加热器22使物镜1的温度高于高湿度空间的气体温度。

6. 发明申请

US20060176367A1 Photo-micrographing device and its control method 有权
标题翻译：照相显影装置及其控制方法
公开(公告)号：US20060176367A1
公开(公告)日：2006-08-10
申请号：US11330874
申请日：2006-01-11
申请人： Hideaki Endo , Akitsugu Kagayama , Katsuyoshi Yamaguchi
发明人： Hideaki Endo , Akitsugu Kagayama , Katsuyoshi Yamaguchi
IPC分类号： H04N7/18 , H04N9/47
CPC分类号： G02B21/367 , G02B21/26
摘要： By comprising a microscope, means for obtaining an inspection object image, means for setting the view field of the microscope, and storing the position information of a stage corresponding to the view field, means for storing setting information of the microscope and camera unit in the view field, means for storing shooting conditions in the case where time-lapse shooting is applied to the view field, means for obtaining a time-lapse shooting image for each view field, based on the stored position information, setting information stored and shooting conditions and means for collectively displaying the latest taken time-lapse shooting images for each view field, a Photo-micrographing device and its control method capable of shooting each inspection object without being restricted by a shooting waiting time and the like when applying multi-point time-lapse shooting to a plurality of shooting ranges can be provided.
摘要翻译：通过包括显微镜，用于获得检查对象图像的装置，用于设置显微镜的视野的装置，以及存储与视野相对应的台的位置信息的装置，用于将显微镜和相机单元的设置信息存储在视场，用于在对视场施加延时拍摄的情况下存储拍摄条件的装置，用于根据所存储的位置信息，存储的设置信息和拍摄条件获得每个视野的延时拍摄图像的装置以及用于集体显示针对每个视野的最新拍摄的延时拍摄图像的装置，能够在不受施加多点时间的拍摄等待时间等的限制的情况下拍摄每个检查对象的照相显影装置及其控制方法可以提供多次拍摄范围的拍摄。

7. 发明申请

US20060092506A1 Microscope and method of preventing dew condensation on objective lens 有权
标题翻译：显微镜及防止物镜结露的方法
公开(公告)号：US20060092506A1
公开(公告)日：2006-05-04
申请号：US11259392
申请日：2005-10-26
申请人： Atsuhiro Tsuchiya , Katsuyoshi Yamaguchi , Akitsugu Kagayama , Kazuhiro Hasegawa , Hideaki Endo , Kenichi Koyama
发明人： Atsuhiro Tsuchiya , Katsuyoshi Yamaguchi , Akitsugu Kagayama , Kazuhiro Hasegawa , Hideaki Endo , Kenichi Koyama
IPC分类号： G02B21/26
CPC分类号： G02B27/0006 , C12M41/12 , C12M41/36 , G02B7/008 , G02B21/30
摘要： A microscope includes a high humidity space that is maintained at a predetermined gas temperature and a predetermined relative humidity for culture of a living sample; an objective lens having a tip portion with a top lens which is disposed in the high humidity space, and serving to allow for an observation of the living cell incubated inside the high humidity space; a heater that heats the objective lens; and a temperature controller that controls the heater so that a surface temperature and an internal temperature of the objective lens are higher than a dew-point temperature and lower than a thermal death point of the living sample.
摘要翻译：显微镜包括保持在预定气体温度和预定相对湿度下的高湿度空间，用于培养活体样品; 物镜，其具有顶部部分，其具有设置在高湿度空间中的顶部透镜，并且用于允许观察在高湿度空间内孵育的活细胞; 加热物镜的加热器; 以及温度控制器，其控制加热器，使得物镜的表面温度和内部温度高于露点温度并且低于活体样品的热死亡点。

8. 发明授权

US07910355B2 Culture observation apparatus 有权
标题翻译：文化观察仪器
公开(公告)号：US07910355B2
公开(公告)日：2011-03-22
申请号：US11318416
申请日：2005-12-22
申请人： Atsuhiro Tsuchiya , Ryuichi Hirano , Hideaki Endo , Kenichi Koyama , Kazuhiro Hasegawa , Akitsugu Kagayama , Katsuyoshi Yamaguchi
发明人： Atsuhiro Tsuchiya , Ryuichi Hirano , Hideaki Endo , Kenichi Koyama , Kazuhiro Hasegawa , Akitsugu Kagayama , Katsuyoshi Yamaguchi
IPC分类号： C12M1/34 , C12M3/00 , G02B21/26 , G02B21/34 , G01N21/01
CPC分类号： C12M41/36 , C12M41/14 , G02B7/22 , G02B21/0088 , G02B21/02
摘要： A culture observation apparatus, which is used for observing a cultured cell while culturing the cell, includes a culture device that cultures the cultured cell, and a microscope used for observing the cultured cell. The culture device includes a first space that is controlled to an environment suitable for culture of the cell, and a second space that is controlled to a lower humidity condition in comparison with the first space. The microscope includes an objective optical unit including an objective lens; the objective optical unit having at least one portion located in the second space, and another portion extending into the first space through an opening formed in a partition wall that separates the first space and the second space, with a gap between the objective optical unit and the partition wall being sealed by a sealing member, and the objective lens includes a plurality of lens groups, with mutual spaces among the lens groups vented to the second space.
摘要翻译：用于在培养细胞时观察培养细胞的培养观察装置包括培养培养细胞的培养装置和用于观察培养细胞的显微镜。培养装置包括被控制到适于细胞培养的环境的第一空间和与第一空间相比被控制到较低湿度条件的第二空间。显微镜包括物镜光学单元，包括物镜; 所述物镜光学单元具有位于所述第二空间中的至少一个部分，以及另一部分，其通过形成在分隔所述第一空间和所述第二空间的分隔壁中的开口延伸到所述第一空间中，所述间隔物在所述物镜光学单元和所述分隔壁由密封构件密封，并且所述物镜包括多个透镜组，所述透镜组中的彼此间隔开放到所述第二空间。

9. 发明申请

US20060141613A1 Culture observation apparatus 有权
标题翻译：文化观察仪器
公开(公告)号：US20060141613A1
公开(公告)日：2006-06-29
申请号：US11318416
申请日：2005-12-22
申请人： Atsuhiro Tsuchiya , Ryuichi Hirano , Hideaki Endo , Kenichi Koyama , Kazuhiro Hasegawa , Akitsugu Kagayama , Katsuyoshi Yamaguchi
发明人： Atsuhiro Tsuchiya , Ryuichi Hirano , Hideaki Endo , Kenichi Koyama , Kazuhiro Hasegawa , Akitsugu Kagayama , Katsuyoshi Yamaguchi
IPC分类号： C12M1/34 , G02B21/26 , G02B21/34
CPC分类号： C12M41/36 , C12M41/14 , G02B7/22 , G02B21/0088 , G02B21/02
摘要： A culture observation apparatus, which is used for observing a cultured cell while culturing the cell, includes a culture device that cultures the cultured cell, and a microscope used for observing the cultured cell. The culture device includes a first space that is controlled to an environment suitable for culture of the cell, and a second space that is controlled to a lower humidity condition in comparison with the first space. The microscope includes an objective optical unit including an objective lens; the objective optical unit having at least one portion located in the second space, and another portion extending into the first space through an opening formed in a partition wall that separates the first space and the second space, with a gap between the objective optical unit and the partition wall being sealed by a sealing member, and the objective lens includes a plurality of lens groups, with mutual spaces among the lens groups vented to the second space.
摘要翻译：用于在培养细胞时观察培养细胞的培养观察装置包括培养培养细胞的培养装置和用于观察培养细胞的显微镜。培养装置包括被控制到适于细胞培养的环境的第一空间和与第一空间相比被控制到较低湿度条件的第二空间。显微镜包括物镜光学单元，包括物镜; 所述物镜光学单元具有位于所述第二空间中的至少一个部分，以及另一部分，其通过形成在分隔所述第一空间和所述第二空间的分隔壁中的开口延伸到所述第一空间中，所述间隔物在所述物镜光学单元和所述分隔壁由密封构件密封，并且所述物镜包括多个透镜组，所述透镜组中的彼此间隔开放到所述第二空间。

10. 发明申请

US20060128005A1 Observation apparatus 有权
公开(公告)号：US20060128005A1
公开(公告)日：2006-06-15
申请号：US11302591
申请日：2005-12-12
申请人： Kazuhiro Hasegawa , Akitsugu Kagayama , Hideaki Endo , Ryuichi Hirano , Atsuhiro Tsuchiya , Kenichi Koyama , Katsuyoshi Yamaguchi
发明人： Kazuhiro Hasegawa , Akitsugu Kagayama , Hideaki Endo , Ryuichi Hirano , Atsuhiro Tsuchiya , Kenichi Koyama , Katsuyoshi Yamaguchi
IPC分类号： C12M1/36 , C12M1/34
CPC分类号： C12M23/50 , C12M23/10 , C12M23/48 , C12M41/36
摘要： An observation apparatus includes a sample tray that holds a container housing a cultured cell and a culture medium and covered with a lid; an observation unit that serves for observation of the cultured cell; and a shifting unit that relatively shifts the sample tray and a light axis of the observation unit along a plane that is orthogonal to the light axis of the observation unit, wherein the sample tray includes a container holding unit that holds the container by utilizing an elastic force, and at least one of the lid and one portion of the lid is made detachable, with the container being held in the sample tray by the container holding unit.

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