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1. 发明授权

US06262336B1 Expression of a heterologous protein C in mammary tissue of transgenic animals using a long whey acidic protein promoter 失效
标题翻译：使用长乳清酸性蛋白启动子在转基因动物的乳腺组织中表达异源蛋白C
公开(公告)号：US06262336B1
公开(公告)日：2001-07-17
申请号：US09321831
申请日：1999-05-28
申请人： Henryk Lubon , William N. Drohan , Lothar Hennighausen , William H. Velander
发明人： Henryk Lubon , William N. Drohan , Lothar Hennighausen , William H. Velander
IPC分类号： A01K67027
CPC分类号： C12N15/8509 , A01K67/0275 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/10 , A01K2227/105 , A01K2227/108 , A01K2267/01 , A61K38/00 , C07K14/75 , C07K2319/00 , C07K2319/02 , C12N9/6464 , C12N2830/008 , C12Y304/21069
摘要： An isolated DNA sequence which regulates the expression of a heterologous gene composed of a mouse whey acidic protein promoter having a length of greater than about 2.4 kb extending upstream from the unique KpnI site in the mouse whey acidic protein gene is disclosed. Specifically a mouse whey acidic protein promoter of about 4.1-4.2 kb in length extending upstream from the unique KpnI site is preferred. This mouse whey acid protein promoter is operably linked to a DNA sequence encoding a heterologous polypeptide and used to prepare transgenic non-human mammals expressing the heterologous polypeptide in their milk. Particularly efficient expression of both cDNAs and genomic DNAs encoding heterologous polypeptides was obtained in transgenic non-human mammals using this promoter, known as the long whey acidic protein promoter.
摘要翻译：公开了一种分离的DNA序列，其调节由小鼠乳清酸性蛋白基因中唯一KpnI位点上游延伸的长度大于约2.4kb的小鼠乳清酸性蛋白启动子组成的异源基因的表达。具体地，优选从独特的KpnI位点上游延伸的长度为约4.1-4.2kb的小鼠乳清酸性蛋白启动子。该小鼠乳清蛋白启动子可操作地连接到编码异源多肽的DNA序列，并用于制备在其牛奶中表达异源多肽的转基因非人哺乳动物。使用称为长乳清酸性蛋白启动子的该启动子，在转基因非人哺乳动物中获得编码异源多肽的cDNA和基因组DNA的特别有效的表达。

2. 发明授权

US06730663B2 Targeting gene expression to living tissue using jet injection 有权
标题翻译：使用喷射靶向基因表达生物组织
公开(公告)号：US06730663B2
公开(公告)日：2004-05-04
申请号：US10037616
申请日：2002-01-02
申请人： Priscilla Anne Furth , Lothar Hennighausen
发明人： Priscilla Anne Furth , Lothar Hennighausen
IPC分类号： A61K31713
CPC分类号： C12N15/89 , C12M35/00
摘要： The present invention provides a method of targeting transient gene expression and stable gene expression from the exogenous administration of a DNA sequence, which sequence is less than a complete genome, wherein said DNA sequence encodes RNA and protein, or RNA only, to differentiate tissue of living organisms wherein said DNA sequence through a jet injector technique, and said DNA sequence of less than a complete genome is expressed in a living organism. The present invention further provides a flexible multi-nozzle injector device with a wide surface area to allow molding of the injector nozzle to the surface contours of the tissue. Another aspect of the present invention provides an injection device having a long nozzle for injection of DNA deep into the host tissue. Also, in a further aspect the present invention provides an injector device modified to be used with and/or inject through an endoscopic device.
摘要翻译：本发明提供了从外源性施用DNA序列靶向瞬时基因表达和稳定基因表达的方法，所述DNA序列小于完整基因组，其中所述DNA序列仅编码RNA和蛋白质或RNA，以区分组织生物体，其中通过喷射注射器技术的所述DNA序列和小于完整基因组的所述DNA序列在活生物体中表达。本发明还提供一种具有宽表面积的柔性多喷嘴注射器装置，以允许将喷射器喷嘴模制成组织的表面轮廓。本发明的另一方面提供一种具有用于将DNA深入宿主组织的长喷嘴的注射装置。此外，在另一方面，本发明提供了一种修改为与内窥镜装置一起使用和/或注射的注射器装置。

3. 发明授权

US5998382A Targeting gene expression to living tissue using jet injection 失效
公开(公告)号：US5998382A
公开(公告)日：1999-12-07
申请号：US433265
申请日：1995-05-02
申请人： Priscilla Anne Furth , Lothar Hennighausen
发明人： Priscilla Anne Furth , Lothar Hennighausen
IPC分类号： C12M3/00 , C12N15/89 , A01N43/04
CPC分类号： C12N15/89 , C12M35/00
摘要： The present invention provides a method of targeting transient gene expression and stable gene expression from the exogenous administration of a DNA sequence, which sequence is less than a complete genome, wherein said DNA sequence encodes RNA and protein, or RNA only, to differentiate tissue of living organisms wherein said DNA sequence through a jet injector technique, and said DNA sequence of less than a complete genome is expressed in a living organism. The present invention further provides a flexible multi-nozzle injector device with a wide surface area to allow molding of the injector nozzle to the surface contours of the tissue. Another aspect of the present invention provides an injection device having a long nozzle for injection of DNA deep into the host tissue. Also, in a further aspect the present invention provides an injector device modified to be used with and/or inject through an endoscopic device.

4. 发明授权

US06361991B1 Targeting gene expression to living tissue using jet injection 失效
公开(公告)号：US06361991B1
公开(公告)日：2002-03-26
申请号：US09433599
申请日：1999-11-02
申请人： Priscilla Anne Furth , Lothar Hennighausen
发明人： Priscilla Anne Furth , Lothar Hennighausen
IPC分类号： C12M300
CPC分类号： C12N15/89 , C12M35/00
摘要： The present invention provides a method of targeting transient gene expression and stable gene expression from the exogenous administration of a DNA sequence, which sequence is less than a complete genome, wherein said DNA sequence encodes RNA and protein, or RNA only, to differentiate tissue of living organisms wherein said DNA sequence through a jet injector technique, and said DNA sequence of less than a complete genome is expressed in a living organism. The present invention further provides a flexible multi-nozzle injector device with a wide surface area to allow molding of the injector nozzle to the surface contours of the tissue. Another aspect of the present invention provides an injection device having a long nozzle for injection of DNA deep into the host tissue. Also, in a further aspect the present invention provides an injector device modified to be used with and/or inject through an endoscopic device.

5. 发明授权

US06727405B1 Transgenic animals secreting desired proteins into milk 失效
标题翻译：将所需蛋白质分泌到牛奶中的转基因动物
公开(公告)号：US06727405B1
公开(公告)日：2004-04-27
申请号：US08246259
申请日：1994-05-19
申请人： Katherine Gordon , Suzanne Groet , Lothar Hennighausen , Heiner Westphal
发明人： Katherine Gordon , Suzanne Groet , Lothar Hennighausen , Heiner Westphal
IPC分类号： A01K67027
CPC分类号： C12N15/8509 , A01K2217/05 , A01K2227/10 , A01K2267/01 , C07K14/005 , C12N9/6459 , C12N15/85 , C12N15/89 , C12N2730/10122 , C12N2830/008 , C12N2830/85 , C12Y304/21069
摘要： A DNA sequence containing a gene encoding a protein, the gene being under the transcriptional control in the DNA sequence of a mammalian milk protein promoter which does not naturally control the transcription of the gene, such DNA sequence including DNA enabling secretion of the protein.
摘要翻译：包含编码蛋白质的基因的DNA序列，所述基因在哺乳动物乳蛋白质启动子的DNA序列中的转录控制下，该哺乳动物乳蛋白启动子不天然地控制基因的转录，所述DNA序列包括能够分泌蛋白质的DNA。

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