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1. 发明授权

US5756344A DNA coding for a human vasoconstrictive peptide and use thereof 失效
标题翻译：编码人血管收缩肽的DNA及其用途
公开(公告)号：US5756344A
公开(公告)日：1998-05-26
申请号：US673269
申请日：1996-06-28
申请人： Haruo Onda , Shoichi Ohkubo , Takuo Kosaka
发明人： Haruo Onda , Shoichi Ohkubo , Takuo Kosaka
IPC分类号： A61K38/00 , C07K14/00 , C07K14/575 , C12N1/21 , C12N15/09 , C12N15/12 , C12N15/16 , C12P21/02
CPC分类号： C07K14/57536 , A61K38/00
摘要： Disclosed are (1) a DNA containing a cDNA segment coding for human endothelin-2 (SEQ ID NO:1), (2) a precursor of human endothelin-2 (SEQ ID NO:2), (3) a transformant carrying a DNA containing a cDNA segment coding for human endothelin-2, and (4) a method for preparing mature human endothelin-2 which comprises culturing the transformant described in (3), accumulating a protein in a culture medium, and collecting the same, whereby human endothelin-2 and the precursors thereof can be produced in large amounts.
摘要翻译：公开了（1）含有编码人内皮素-2（SEQ ID NO：1）的cDNA片段的DNA，（2）人内皮素-2（SEQ ID NO：2）的前体，（3）携带含有编码人内皮素-2的cDNA片段的DNA，（4）成熟人内皮素-2的制备方法，其包括培养（3）所述的转化体，在培养基中积聚蛋白质，收集培养基，由此人内皮素-2及其前体可以大量生产。

2. 发明授权

US5521069A Genomic DNA exons having exons encoding human pituitary adenylate cyclase activity peptide with 38 amino acids residues(PACAP38) and a promoter thereof 失效
标题翻译：具有编码具有38个氨基酸残基的人垂体腺苷酸环化酶活性肽（PACAP38）的外显子的基因组DNA外显子及其启动子
公开(公告)号：US5521069A
公开(公告)日：1996-05-28
申请号：US76011
申请日：1993-06-11
申请人： Haruo Onda , Chiharu Kimura , Shoichi Ohkubo
发明人： Haruo Onda , Chiharu Kimura , Shoichi Ohkubo
IPC分类号： C07K14/575 , C12N15/85 , C12N5/10
CPC分类号： C07K14/57563
摘要： Disclosed are (1) a genomic DNA of human PACAP38; (2) a DNA containing a DNA segment coding for human PACAP38; (3) a DNA of human PACAP 38 promotor; (4) a transformant carrying a vector which contains a DNA of (3) or further contains a DNA coding for a protein downstream from the promotor; and (5) a method for preparing a protein comprising cultivating the transformant described in the above (4), accumulating the protein in a culture product, and collecting the resulting protein such as mature PACAP 38. The DNA gives human PACAP 38 effectively and makes it possible to screen the chemical substance necessary for production of PACAP and is applied to experimental animals to understand their brain functions, which serves to elucidate human brain functions. Human PACAP38 can also be utilized as therapeutic agents about growth and maintenance of human brain nerves.
摘要翻译：公开的是（1）人PACAP38的基因组DNA; （2）含有编码人PACAP38的DNA片段的DNA; （3）人PACAP 38启动子的DNA; （4）携带载体的转化体，其含有（3）的DNA或进一步含有编码来自启动子下游的蛋白质的DNA; （5）一种制备蛋白质的方法，包括培养上述（4）中所述的转化体，将蛋白质积聚在培养产物中，并收集所得蛋白质如成熟PACAP 38.该DNA有效地给予人PACAP 38，并使可以筛选生产PACAP所需的化学物质，并将其应用于实验动物，以了解其大脑功能，从而阐明人脑功能。人类PACAP38也可以用作关于人脑神经生长和维持的治疗剂。

3. 发明授权

US06365725B1 DNA coding for a human vasoconstrictive peptide and use thereof 失效
标题翻译：编码人血管收缩肽的DNA及其用途
公开(公告)号：US06365725B1
公开(公告)日：2002-04-02
申请号：US07728169
申请日：1991-07-10
申请人： Haruo Onda , Shoichi Ohkubo
发明人： Haruo Onda , Shoichi Ohkubo
IPC分类号： C12N1512
CPC分类号： C07K14/57536 , A61K38/00
摘要： Disclosed are (1) a DNA containing a cDNA segment coding for human endothelin-2 (SEQ ID NO:1), (2) a precursor of human endothelin-2 (SEQ ID NO:2), (3) a transformant carrying a DNA containing a cDNA segment coding for human endothelin-2, and (4) a method for preparing mature human endothelin-2 which comprises culturing the transformant described in (3), accumulating a protein in a culture medium, and collecting the same, whereby human endothelin-2 and the precursors thereof can be produced in large amounts.
摘要翻译：公开了（1）含有编码人内皮素-2（SEQ ID NO：1）的cDNA片段的DNA，（2）人内皮素-2（SEQ ID NO：2）的前体，（3）携带含有编码人内皮素-2的cDNA片段的DNA，（4）成熟人内皮素-2的制备方法，其包括培养（3）所述的转化体，在培养基中积聚蛋白质，收集培养基，由此人内皮素-2及其前体可以大量生产。

4. 发明授权

US6117645A Human LH-RH receptor expression cells and use thereof 失效
标题翻译：人LH-RH受体表达细胞及其用途
公开(公告)号：US6117645A
公开(公告)日：2000-09-12
申请号：US867260
申请日：1997-06-02
申请人： Haruo Onda , Shoichi Ohkubo , Shuji Hinuma
发明人： Haruo Onda , Shoichi Ohkubo , Shuji Hinuma
IPC分类号： A61K38/00 , A61K39/00 , C07K7/23 , C07K14/72 , C12N15/12 , G01N33/53
CPC分类号： C07K7/23 , C07K14/723 , A61K38/00 , A61K39/00
摘要： Disclosed are CHO cells which are capable of continued production of human LH-RH receptor proteins, or cell membrane fractions thereof; recombinant human LH-RH receptor proteins or partial peptides thereof; methods for screening compounds which have affinity for an LH-RH receptor by contacting the compound with the CHO cells or the cell membrane fractions thereof, or the recombinant human LH-RH receptor proteins or the partial peptides thereof; kits for screening them; the compounds which have affinity for the LH-RH receptor obtained by methods for the screening or kits for the screening; and pharmaceutical compositions containing the compound, thereby being able to early provide prophylactic or therapeutic compositions, for example, for prostate cancer, uterine cancer, breast cancer, a pituitary tumor, endometriosis, hysteromyoma or precocious puberty. They are also useful as a pregnancy controlling composition such as contraceptive or a menstrual cycle controlling composition.
摘要翻译：公开了能够连续产生人LH-RH受体蛋白质或其细胞膜级分的CHO细胞; 重组人LH-RH受体蛋白或其部分肽; 通过使化合物与CHO细胞或其细胞膜级分或重组人LH-RH受体蛋白质或其部分肽接触来筛选对LH-RH受体具有亲和性的化合物的方法; 用于筛选它们的试剂盒通过筛选方法获得的对LH-RH受体具有亲和力的化合物或用于筛选的试剂盒; 以及含有该化合物的药物组合物，从而能够早期提供例如前列腺癌，子宫癌，乳腺癌，垂体瘤，子宫内膜异位症，子宫肌瘤或早熟青春期的预防或治疗组合物。它们也可用作妊娠控制组合物，例如避孕或月经周期控制组合物。

5. 发明授权

US5677184A CHO cells that express human LH-RH receptor 失效
标题翻译：表达人LH-RH受体的CHO细胞
公开(公告)号：US5677184A
公开(公告)日：1997-10-14
申请号：US423691
申请日：1995-04-18
申请人： Haruo Onda , Shoichi Ohkubo , Shuji Hinuma , Hidekazu Sawada
发明人： Haruo Onda , Shoichi Ohkubo , Shuji Hinuma , Hidekazu Sawada
IPC分类号： A61K38/00 , A61K39/00 , C07K7/23 , C07K14/72 , C12N15/12 , G01N33/53 , C12N15/85
CPC分类号： C07K7/23 , C07K14/723 , A61K38/00 , A61K39/00
摘要： Disclosed are CHO cells which are capable of continued production of human LH-RH receptor proteins, or cell membrane fractions thereof; recombinant human LH-RH receptor proteins or partial peptides thereof; methods for screening compounds which have affinity for an LH-RH receptor by contacting the compound with the CHO cells or the cell membrane fractions thereof, or the recombinant human LH-RH receptor proteins or the partial peptides thereof; kits for screening them; the compounds which have affinity for the LH-RH receptor obtained by methods for the screening or kits for the screening; and pharmaceutical compositions containing the compound, thereby being able to early provide prophylactic or therapeutic compositions, for example, for prostate cancer, uterine cancer, breast cancer, a pituitary tumor, endometriosis, hysteromyoma or precocious puberty. They are also useful as a pregnancy controlling composition such as contraceptive or a menstrual cycle controlling composition.
摘要翻译：公开了能够连续产生人LH-RH受体蛋白质或其细胞膜级分的CHO细胞; 重组人LH-RH受体蛋白或其部分肽; 通过使化合物与CHO细胞或其细胞膜级分或重组人LH-RH受体蛋白或其部分肽接触来筛选对LH-RH受体具有亲和力的化合物的方法; 用于筛选它们的试剂盒通过筛选方法获得的对LH-RH受体具有亲和力的化合物或用于筛选的试剂盒; 以及含有该化合物的药物组合物，从而能够早期提供例如前列腺癌，子宫癌，乳腺癌，垂体瘤，子宫内膜异位症，子宫肌瘤或早熟青春期的预防或治疗组合物。它们也可用作妊娠控制组合物，例如避孕或月经周期控制组合物。

6. 发明授权

US5858787A DNA encoding PACAP receptor protein and method for preparing said protein 失效
标题翻译：编码PACAP受体蛋白的DNA及其制备方法
公开(公告)号：US5858787A
公开(公告)日：1999-01-12
申请号：US811897
申请日：1997-03-05
申请人： Haruo Onda , Masaki Hosoya , Kazuhiro Ogi , Yasunori Miyamoto , Yugo Habata
发明人： Haruo Onda , Masaki Hosoya , Kazuhiro Ogi , Yasunori Miyamoto , Yugo Habata
IPC分类号： A61K38/00 , C07K14/575 , C07K14/72 , C12N1/21 , C12N15/12 , C12P21/08 , C12N5/10 , C12N15/63
CPC分类号： C07K14/57563 , C07K14/72 , A61K38/00
摘要： This invention discloses a PACAP receptor protein or a salt thereof, a DNA comprising a DNA fragment coding for the protein, a method for preparing the protein, antibody against the protein and use of the protein, DNA and antibodies. A PACAP receptor protein was purified from the bovine cerebrum. DNAs coding for PACAP receptor proteins were isolated from bovine, rat and human cDNA libraries, and their nucleotide sequences were determined. The PACAP receptor proteins and the DNAs coding for the proteins of the present invention can be used for (1) acquisition of antibodies and antisera, (2) construction of expression systems of recombinant receptor proteins, (3) development of receptor binding assay systems using said expression systems and screening of potential compounds for drugs, (4) execution of drug design based on the comparison of ligands and receptors which are structurally similar to each other, (5) preparation of probes and PCR primers in gene diagnosis, and the like.
摘要翻译：本发明公开了一种PACAP受体蛋白质或其盐，包含编码该蛋白质的DNA片段的DNA，该蛋白质的制备方法，针对该蛋白质的抗体及该蛋白质，DNA及抗体的用途。从牛大脑中纯化PACAP受体蛋白。从牛，大鼠和人类cDNA文库中分离编码PACAP受体蛋白的DNA，测定其核苷酸序列。 PACAP受体蛋白和编码本发明蛋白的DNA可用于（1）获得抗体和抗血清，（2）构建重组受体蛋白质的表达系统，（3）开发受体结合测定系统，使用表达系统和药物潜在化合物的筛选，（4）基于结构相似的配体和受体的比较进行药物设计，（5）基因诊断中探针和PCR引物的制备等。

7. 发明授权

US06399325B1 DNA encoding a galanin receptor 失效
标题翻译：编码甘丙肽受体的DNA
公开(公告)号：US06399325B1
公开(公告)日：2002-06-04
申请号：US08540650
申请日：1995-10-11
申请人： Shuji Hinuma , Ryo Fujii , Shoji Fukusumi , Tetsuya Ohtaki , Masaki Hosoya , Kazuhiro Ohgi , Haruo Onda
发明人： Shuji Hinuma , Ryo Fujii , Shoji Fukusumi , Tetsuya Ohtaki , Masaki Hosoya , Kazuhiro Ohgi , Haruo Onda
IPC分类号： C12N1512
CPC分类号： C07K14/723
摘要： Galanin receptor proteins, production and use thereof including screening of galanin receptor agonists and antagonists are provided. Galanin receptor proteins, etc. or salts thereof, partial peptides thereof, DNAs coding for the above galanin receptor protein, processes for producing the above receptor protein, methods of screening for a galanin receptor agonist and/or antagonist or screening kits therefor, agonist and/or antagonist compounds or salts thereof obtained by the above screening method or the screening kit, pharmaceutical compositions containing the above compound or its salt, and antibodies against the above receptor protein are provided. It is allowable to efficiently screen a galanin receptor agonist or antagonist by using the galanin receptor protein, the partial peptide thereof, the galanin receptor protein-encoding DNA, the receptor protein-containing cell or its membrane fraction. The pharmaceuticals thus screened or characterized permits various applications including prophylactic and/or therapeutic treatments against a variety of diseases, e.g., stomach ulcer, diabetes, Alzheimer's disease, dementia, etc. and a sedative.
摘要翻译：提供了Galanin受体蛋白质，其生产和使用，包括甘露糖素受体激动剂和拮抗剂的筛选。加兰林素受体蛋白等或其盐，其部分肽，编码上述甘丙肽受体蛋白质的DNA，产生上述受体蛋白质的方法，筛选甘丙肽受体激动剂和/或拮抗剂的方法或其筛选试剂盒，激动剂和 /或通过上述筛选方法获得的拮抗剂化合物或其盐或筛选试剂盒，含有上述化合物或其盐的药物组合物和抗上述受体蛋白的抗体。可以通过使用甘丙肽受体蛋白，其部分肽，编码甘露糖素受体蛋白的DNA，含受体蛋白的细胞或其膜部分来有效筛选甘丙肽受体激动剂或拮抗剂。如此筛选或表征的药物允许各种应用，包括针对各种疾病（例如胃溃疡，糖尿病，阿尔茨海默病，痴呆等）和镇静剂的预防和/或治疗性治疗。

8. 发明授权

US06399316B1 PACAP receptor protein, method for preparing said protein, and use thereof 失效
标题翻译： PACAP受体蛋白质，所述蛋白质的制备方法及其用途
公开(公告)号：US06399316B1
公开(公告)日：2002-06-04
申请号：US09201474
申请日：1998-11-30
申请人： Haruo Onda , Tetsuya Ohtaki , Yasushi Masuda , Chieko Kitada , Yoshihiro Ishibashi , Masaki Hosoya , Kazuhiro Ogi , Yasunori Miyamoto , Yugo Habata , Norio Shimamoto
发明人： Haruo Onda , Tetsuya Ohtaki , Yasushi Masuda , Chieko Kitada , Yoshihiro Ishibashi , Masaki Hosoya , Kazuhiro Ogi , Yasunori Miyamoto , Yugo Habata , Norio Shimamoto
IPC分类号： G01N3353
CPC分类号： C07K14/72 , A61K38/00 , A61K48/00
摘要： This invention discloses a PACAP receptor protein or a salt thereof, a DNA comprising a DNA fragment coding for the protein, a method for preparing the protein, antibody against the protein, and use of the protein, DNA, and antibodies. A PACAP receptor protein was purified from the bovine cerebrum. DNAs coding for PACAP receptor proteins were isolated from bovine, rat and human cDNA libraries, and their nucleotide sequences were determined. Further, amino acid sequences deduced from the nucleotide sequences were determined. The PACAP receptor proteins and the DNAs coding for the proteins of the present invention can be used for (1) acquisition of antibodies and antisera, (2) construction of expression systems of recombinant receptor proteins, (3) development of receptor binding assay systems using said expression systems and screening of potential compounds for drugs, (4) execution of drug design based on the comparison of ligands and receptors which are structurally similar to each other, (5) preparation of probes and PCR primers in gene diagnosis, and the like.
摘要翻译：本发明公开了一种PACAP受体蛋白或其盐，包含编码该蛋白质的DNA片段的DNA，该蛋白质的制备方法，针对该蛋白质的抗体以及该蛋白质，DNA及抗体的用途。从牛大脑中纯化PACAP受体蛋白。从牛，大鼠和人类cDNA文库中分离编码PACAP受体蛋白的DNA，测定其核苷酸序列。此外，确定从核苷酸序列推导的氨基酸序列。 PACAP受体蛋白和编码本发明蛋白的DNA可用于（1）获得抗体和抗血清，（2）构建重组受体蛋白质的表达系统，（3）开发受体结合测定系统，使用表达系统和药物潜在化合物的筛选，（4）基于结构相似的配体和受体的比较来执行药物设计，（5）基因诊断中探针和PCR引物的制备等。

9. 发明授权

US5879896A Method of screening for inhibitors of human thyrotropin releasing hormone (TRH) receptor 失效
标题翻译：筛选人类促甲状腺激素释放激素（TRH）受体抑制剂的方法
公开(公告)号：US5879896A
公开(公告)日：1999-03-09
申请号：US288663
申请日：1994-08-09
申请人： Shuji Hinuma , Masaki Hosoya , Haruo Onda
发明人： Shuji Hinuma , Masaki Hosoya , Haruo Onda
IPC分类号： A61K38/00 , C07K14/72 , C07K16/28 , C12N15/12 , G01N33/76 , G01N33/53 , G01N33/566
CPC分类号： G01N33/76 , C07K14/72 , C07K16/2869 , A61K38/00
摘要： A human receptor protein capable of binding TRH, a DNA coding for said protein, use of the proetein and DNA, a method for preparing said protein, and antibodies to the protein are described.The human TRH receptor protein and the DNA coding for the protein of the present invention are useful as (1) a diagnostic composition for neuropathy (particularly, dementia), (2) a pharmaceutical composition for neuropathy and (3) a material used for screening a TRH receptor agonist or antagonist.
摘要翻译：描述了能够结合TRH的人受体蛋白质，编码所述蛋白质的DNA，使用原蛋白和DNA，制备所述蛋白质的方法和对蛋白质的抗体。人TRH受体蛋白质和编码本发明蛋白质的DNA可用作（1）神经病（特别是痴呆）的诊断用组合物，（2）神经病的药物组合物和（3）用于筛选的材料 TRH受体激动剂或拮抗剂。

10. 发明授权

US5756295A DNA primer and a method for screening DNAS 失效
标题翻译： DNA引物和DNAS筛选方法
公开(公告)号：US5756295A
公开(公告)日：1998-05-26
申请号：US566037
申请日：1995-12-01
申请人： Haruo Onda , Masaki Hosoya
发明人： Haruo Onda , Masaki Hosoya
IPC分类号： C07K5/083 , C07K14/575 , C12Q1/68 , C07H21/02
CPC分类号： C12Q1/6876 , C07K14/57536 , C07K5/0806
摘要： DNA primers each having a nucleotide sequence substantially complementary to a nucleotide sequence which codes for Gly-Lys-Arg, Gly-Lys-Lys or Gly-Arg-Arg; methods for amplifying a polynucleotide which codes for a peptide or a precursor protein thereof using said DNA primer; screening methods for a polynucleotide which codes for a peptide comprising an amino acid sequence of Gly-Lys-Arg, Gly-Lys-Lys or Gly-Arg-Arg or a precursor protein thereof from a DNA library, etc. using said DNA; the polynucleotide obtained by said screening method; and the peptide or its precursor protein encoded by said polynucleotide, or a salt thereof. When the DNA primer of the present invention is used, the DNA which codes for a peptide having a useful physiological activity or a precursor protein thereof can be efficiently cloned.
摘要翻译： DNA引物各自具有与编码Gly-Lys-Arg，Gly-Lys-Lys或Gly-Arg-Arg的核苷酸序列基本上互补的核苷酸序列; 使用所述DNA引物扩增编码肽或其前体蛋白的多核苷酸的方法; 使用所述DNA从DNA文库等编码包含Gly-Lys-Arg，Gly-Lys-Lys或Gly-Arg-Arg或其前体蛋白质的氨基酸序列的肽的多核苷酸的筛选方法; 通过所述筛选方法获得的多核苷酸; 和由所述多核苷酸编码的肽或其前体蛋白质或其盐。当使用本发明的DNA引物时，可以有效地克隆编码具有有用生理活性的肽或其前体蛋白质的DNA。

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